Rh1DG035000

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018203
Physical Location & Seq
Reverse (-)
5218435 .. 5219176
742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG035000.1

Sequence Viewer

Length: 330 bp
ATGTCTACCGGAATCGTGACCCGGTTCAAGGAAGCCGGCCAATCGACGATGGCGGCGCACCAGCTGTGGTCTGAATTCACTGACTCGACCGCCCTCAGCCTCCCTTCAAGCCTCTTCGACGCCTCGACCCGACCGACGAAGCCGGCGCCACTGGTGACGATACTCCTGAGCCCGCGCCTTGCCGAGGCAGGCAGGAACCTTGCAGGTGTTGTTGCGGTGGTCGGTGGAGAAGGAGCCTCCAAGGTTACGTCTGTTTTCTGGAAGGAGAATGAGCTAAACATGGTTGATACGCCGGGACATGCAGACTTTGGTGGTGAAGTATGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

109

Amino Acids

11.46

Weight (kDa)

5.48

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 80 - 107 9.1e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018203)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0275271
rosa_laevigata RLG00000024622
rosa_multiflora Rmu_sc0003465.1_g000049
rosa_roxburghii Rroxscaffold_4G00319090
rosa_rugosa Rorug02G0141600
rosa_samantha Rh1DG035000 Rh4BG150800 Rh4BG192300 Rh4CG279100 Rh5AG431300 Rh5DG460100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 175
AciI CCGC 4 cut(s) 53, 90, 173, 215
AcoI YGGCCR 1 cut(s) 37
AcsI RAATTY 1 cut(s) 74
AcyI GRCGYC 2 cut(s) 120, 146
AfiI CCNNNNNNNGG 2 cut(s) 28, 184
AgsI TTSAA 2 cut(s) 28, 108
AluBI AGCT 2 cut(s) 64, 274
AluI AGCT 2 cut(s) 64, 274
AoxI GGCC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 74
AspLEI GCGC 3 cut(s) 58, 148, 177
AsuC2I CCSGG 2 cut(s) 22, 294
AsuHPI GGTGA 2 cut(s) 166, 326
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 173
BbvCI CCTCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 43
BcnI CCSGG 2 cut(s) 22, 294
BfaI CTAG 1 cut(s) 328
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 22, 294
BmiI GGNNCC 3 cut(s) 147, 197, 235
BmrFI CCNGG 2 cut(s) 22, 294
Bpu10I CCTNAGC 2 cut(s) 95, 167
BpuMI CCSGG 2 cut(s) 22, 294
BsaHI GRCGYC 2 cut(s) 120, 146
BsaJI CCNNGG 2 cut(s) 183, 240
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 184
Bse118I RCCGGY 2 cut(s) 35, 142
Bse1I ACTGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 183, 240
BseLI CCNNNNNNNGG 2 cut(s) 28, 184
BseMII CTCAG 2 cut(s) 109, 158
BseNI ACTGG 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 2 cut(s) 90, 134
BshFI GGCC 1 cut(s) 39
BshNI GGYRCC 1 cut(s) 145
BsiEI CGRYCG 2 cut(s) 90, 134
BsiSI CCGG 5 cut(s) 9, 22, 36, 143, 293
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 28, 184
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 173
BspACI CCGC 4 cut(s) 53, 90, 173, 215
BspANI GGCC 1 cut(s) 39
BspCNI CTCAG 2 cut(s) 108, 159
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 3 cut(s) 147, 197, 235
BspMI ACCTGC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 145
BsrFI RCCGGY 2 cut(s) 35, 142
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 2 cut(s) 35, 142
BssECI CCNNGG 2 cut(s) 183, 240
BssNI GRCGYC 2 cut(s) 120, 146
BssT1I CCWWGG 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 2 cut(s) 120, 146
BstC8I GCNNGC 4 cut(s) 37, 144, 173, 190
BstDEI CTNAG 2 cut(s) 95, 167
BstENI CCTNNNNNAGG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 58, 148, 177
BstMCI CGRYCG 2 cut(s) 90, 134
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 20, 292
BstUI CGCG 1 cut(s) 175
BsuRI GGCC 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 78, 149
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 4 cut(s) 37, 144, 173, 190
CfoI GCGC 3 cut(s) 58, 148, 177
Cfr10I RCCGGY 2 cut(s) 35, 142
CseI GACGC 1 cut(s) 128
CviAII CATG 2 cut(s) 280, 299
CviJI RGCY 9 cut(s) 35, 39, 64, 99, 111, 142, 171, 236, 274
CviKI_1 RGCY 9 cut(s) 35, 39, 64, 99, 111, 142, 171, 236, 274
DdeI CTNAG 2 cut(s) 95, 167
DinI GGCGCC 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 147
EheI GGCGCC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 283, 302
FaiI YATR 3 cut(s) 281, 300, 322
FaqI GGGAC 1 cut(s) 309
FatI CATG 2 cut(s) 279, 298
FauI CCCGC 1 cut(s) 180
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 328
GlaI GCGC 3 cut(s) 57, 147, 176
GluI GCNGC 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 39
HapII CCGG 5 cut(s) 9, 22, 36, 143, 293
HgaI GACGC 1 cut(s) 128
HhaI GCGC 3 cut(s) 58, 148, 177
Hin1I GRCGYC 2 cut(s) 120, 146
Hin1II CATG 2 cut(s) 283, 302
Hin6I GCGC 3 cut(s) 56, 146, 175
HinP1I GCGC 3 cut(s) 56, 146, 175
HinfI GANTC 2 cut(s) 12, 83
HpaII CCGG 5 cut(s) 9, 22, 36, 143, 293
HphI GGTGA 2 cut(s) 166, 326
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 16, 166, 259
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 49, 122, 139
HpyAV CCTTC 3 cut(s) 114, 224, 256
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 2 cut(s) 203, 302
HpyF3I CTNAG 2 cut(s) 95, 167
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 2 cut(s) 120, 146
Hsp92II CATG 2 cut(s) 283, 302
HspAI GCGC 3 cut(s) 56, 146, 175
KasI GGCGCC 1 cut(s) 145
KroI GCCGGC 2 cut(s) 35, 142
KroNI GCCGGC 2 cut(s) 37, 144
LmnI GCTCC 1 cut(s) 233
MaeI CTAG 1 cut(s) 328
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 3 cut(s) 16, 154, 244
MboII GAAGA 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 1 cut(s) 74
Mly113I GGCGCC 1 cut(s) 146
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 6 cut(s) 104, 110, 122, 133, 178, 247
MroNI GCCGGC 2 cut(s) 35, 142
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 5 cut(s) 9, 22, 36, 143, 293
MspR9I CCNGG 2 cut(s) 22, 294
MvnI CGCG 1 cut(s) 175
NaeI GCCGGC 2 cut(s) 37, 144
NarI GGCGCC 1 cut(s) 146
NciI CCSGG 2 cut(s) 22, 294
NgoMIV GCCGGC 2 cut(s) 35, 142
NlaIII CATG 2 cut(s) 283, 302
NlaIV GGNNCC 3 cut(s) 147, 197, 235
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 16, 154
NspI RCATGY 1 cut(s) 302
PaqCI CACCTGC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PdiI GCCGGC 2 cut(s) 37, 144
PfeI GAWTC 1 cut(s) 12
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 77
PluTI GGCGCC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 77
PspN4I GGNNCC 3 cut(s) 147, 197, 235
PvuII CAGCTG 1 cut(s) 64
SatI GCNGC 1 cut(s) 54
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 22, 294
SduI GDGCHC 1 cut(s) 173
SetI ASST 6 cut(s) 66, 201, 208, 246, 251, 276
SfoI GGCGCC 1 cut(s) 147
Sse9I AATT 1 cut(s) 74
SsiI CCGC 4 cut(s) 53, 90, 173, 215
SspDI GGCGCC 1 cut(s) 145
SspMI CTAG 1 cut(s) 328
StyD4I CCNGG 2 cut(s) 20, 292
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 251
TaqI TCGA 4 cut(s) 44, 86, 117, 125
TaqII GACCGA 1 cut(s) 148
TasI AATT 1 cut(s) 74
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 2 cut(s) 85, 156
TseFI GTSAC 2 cut(s) 16, 154
Tsp45I GTSAC 2 cut(s) 16, 154
TspRI CASTG 2 cut(s) 85, 156
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 1 cut(s) 74
XceI RCATGY 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.