Rh4BG150800

Translation factor GUF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0018203
Physical Location & Seq
Forward (+)
26951623 .. 26952180
558 bp
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UTR
Exon/CDS
Intron
Rh4BG150800.1

Sequence Viewer

Length: 270 bp
ATGATGATGATCCTGAGCCTGCGCCTTGCCGAGGCTGGCAGGAACCTTGTCGGCGTTGTTGCGGAGGTCGGGGGAGAAGGAGCCTCCAAGGTTGCGTCGGTTTTCTGGAAGGAGAATGAGCTAAACATGGTTGATACGCCGGGACATGCAGACTTTGGTGGTGAAGTTGAGCAGGTTGTTGGTATGGTTGAAGGGGCAGTTTTAGTTGTTGATGCCGAGAAAGATCTGCTAGCACAGACCAAGTTTGTGCTTGCCAAAGCCTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

89

Amino Acids

9.39

Weight (kDa)

4.7

Isoelectric Point (pI)

3.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 29 - 88 1.7e-15 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018203)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0275271
rosa_laevigata RLG00000024622
rosa_multiflora Rmu_sc0003465.1_g000049
rosa_roxburghii Rroxscaffold_4G00319090
rosa_rugosa Rorug02G0141600
rosa_samantha Rh1DG035000 Rh4BG150800 Rh4BG192300 Rh4CG279100 Rh5AG431300 Rh5DG460100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 4
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 1 cut(s) 191
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
AlwI GGATC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 24
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 1 cut(s) 173
AsuNHI GCTAGC 1 cut(s) 229
BcnI CCSGG 1 cut(s) 141
BfaI CTAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 163
BglII AGATCT 1 cut(s) 223
Bme1390I CCNGG 1 cut(s) 141
BmiI GGNNCC 2 cut(s) 44, 82
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 202
BmtI GCTAGC 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 30, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 30, 87
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 5
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 9, 223
BspACI CCGC 1 cut(s) 62
BspCNI CTCAG 1 cut(s) 6
BspLI GGNNCC 2 cut(s) 44, 82
BspMI ACCTGC 1 cut(s) 163
BspOI GCTAGC 1 cut(s) 233
BspPI GGATC 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 30, 87
BssMI GATC 2 cut(s) 9, 223
BssT1I CCWWGG 1 cut(s) 87
BstC8I GCNNGC 4 cut(s) 20, 37, 231, 252
BstDEI CTNAG 1 cut(s) 14
BstENI CCTNNNNNAGG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 2 cut(s) 12, 226
BstMBI GATC 2 cut(s) 9, 223
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 4 cut(s) 20, 37, 231, 252
CfoI GCGC 1 cut(s) 24
CseI GACGC 1 cut(s) 84
CviAII CATG 2 cut(s) 127, 146
CviJI RGCY 5 cut(s) 18, 35, 83, 121, 260
CviKI_1 RGCY 5 cut(s) 18, 35, 83, 121, 260
DdeI CTNAG 1 cut(s) 14
DpnI GATC 2 cut(s) 11, 225
DpnII GATC 2 cut(s) 9, 223
Eco130I CCWWGG 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 130, 149
FaiI YATR 3 cut(s) 128, 147, 185
FaqI GGGAC 1 cut(s) 156
FatI CATG 2 cut(s) 126, 145
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 23
HapII CCGG 1 cut(s) 140
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 24
Hin1II CATG 2 cut(s) 130, 149
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 13, 106
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 71, 103, 185
HpyCH4V TGCA 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 14
Hsp92II CATG 2 cut(s) 130, 149
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 2 cut(s) 9, 223
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 7 cut(s) 21, 25, 26, 32, 91, 153, 158
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 230
MalI GATC 2 cut(s) 11, 225
MboI GATC 2 cut(s) 9, 223
MflI RGATCY 1 cut(s) 223
MnlI CCTC 3 cut(s) 25, 58, 94
MseI TTAA 1 cut(s) 263
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 141
NciI CCSGG 1 cut(s) 141
NdeII GATC 2 cut(s) 9, 223
NheI GCTAGC 1 cut(s) 229
NlaIII CATG 2 cut(s) 130, 149
NlaIV GGNNCC 2 cut(s) 44, 82
NmeAIII GCCGAG 2 cut(s) 55, 241
NspI RCATGY 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 44, 82
PsuI RGATCY 1 cut(s) 223
SaqAI TTAA 1 cut(s) 263
Sau3AI GATC 2 cut(s) 9, 223
ScrFI CCNGG 1 cut(s) 141
SetI ASST 5 cut(s) 48, 69, 93, 123, 177
SfaNI GCATC 1 cut(s) 202
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 87
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
XagI CCTNNNNNAGG 1 cut(s) 29
XceI RCATGY 1 cut(s) 149
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.