Rh1DG214900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0019032
Physical Location & Seq
Reverse (-)
42050746 .. 42075913
25168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG214900.1

Sequence Viewer

Length: 501 bp
ATGAAGAGGACGATATTTTTTGCTTTGGTGAGGCTGAGAGACGTTTCTGTTTGGACGTCGTCAGCGTCGAAGCTTTTCGCCACAAGAGATTGTGGTGGCAGAGATGAATTATTCCACCAAAATAAATTCCACTCTAGGTTTTTTTCACTATATGATGTTGGCATGCCAAGTTTCCGAAATGGGTGCAATTTCGCAGCTGGAGGGGCTGCTACACTTCCGGCAACTGCAACATCTGTCTGCCCCTTTTCATTTGGGATTCAGGTCTCCCAGTTTTTGCGATTCAAAGTCCAGGCTTTTCAATTGCTAGCTGCTAAAGGTAAGGAACTTGAGAATTATCTACCCTCAGAAGATTCCTTTCAGAAGGGGCTTTACGTGTTTGATATAGGCCAAAATGATCTTGCTGACAATGTCTATTGGAAGCATGTTGTGCGCCGCATCGAAGCATATAAGGTTGTACTGCATGGAGGACCTAAGTTTGAGGCAAGTAATGTTATCTTAAAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

167

Amino Acids

18.82

Weight (kDa)

9.48

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 52 - 137 1.9e-07 GDSL-like Lipase/Acylhydrolase
Hexapep_UGP3_C PF25441 134 - 167 2.2e-07 UGP3-like, C-terminal hexapeptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019032)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0231861 RchiOBHm_Chr7g0231981
rosa_multiflora Rmu_sc0003385.1_g000003
rosa_samantha Rh1BG185100 Rh1DG073400 Rh1DG214900 Rh5CG209300 Rh6CG200000 Rh7BG397200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 59
AciI CCGC 1 cut(s) 433
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 456
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 2 cut(s) 283, 299
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 3 cut(s) 73, 197, 308
AluI AGCT 3 cut(s) 73, 197, 308
Alw26I GTCTC 2 cut(s) 33, 268
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 194, 206, 308
ApoI RAATTY 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 40
AsuNHI GCTAGC 1 cut(s) 304
AvaII GGWCC 1 cut(s) 467
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbvI GCAGC 3 cut(s) 193, 206, 295
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BciT130I CCWGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 33, 268
BfaI CTAG 2 cut(s) 135, 305
BisI GCNGC 4 cut(s) 195, 207, 309, 433
BlsI GCNGC 4 cut(s) 196, 208, 310, 434
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 467
BmrFI CCNGG 1 cut(s) 290
BmrI ACTGGG 1 cut(s) 262
BmsI GCATC 1 cut(s) 444
BmtI GCTAGC 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 26, 357
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 3 cut(s) 193, 206, 295
BshFI GGCC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 33, 268
BsmBI CGTCTC 1 cut(s) 33
BsnI GGCC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 1 cut(s) 433
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 27, 356
BspOI GCTAGC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 268
BsrI ACTGG 1 cut(s) 268
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 427
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 164, 306
BstDEI CTNAG 3 cut(s) 35, 343, 471
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 2 cut(s) 33, 268
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 2 cut(s) 203, 427
BstNI CCWGG 1 cut(s) 290
BstNSI RCATGY 2 cut(s) 166, 425
BstSCI CCNGG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 193, 206, 295
BsuRI GGCC 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 164, 306
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 455
CviAII CATG 3 cut(s) 163, 422, 461
CviJI RGCY 8 cut(s) 34, 73, 197, 206, 293, 308, 367, 387
CviKI_1 RGCY 8 cut(s) 34, 73, 197, 206, 293, 308, 367, 387
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 3 cut(s) 35, 343, 471
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 3 cut(s) 166, 425, 464
FaiI YATR 8 cut(s) 151, 153, 164, 383, 423, 445, 447, 462
FatI CATG 3 cut(s) 162, 421, 460
Fnu4HI GCNGC 4 cut(s) 195, 207, 309, 433
Fsp4HI GCNGC 4 cut(s) 195, 207, 309, 433
FspBI CTAG 2 cut(s) 135, 305
GlaI GCGC 1 cut(s) 431
GluI GCNGC 4 cut(s) 195, 207, 309, 433
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 387
HapII CCGG 1 cut(s) 218
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 3 cut(s) 166, 425, 464
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 256, 279, 350
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 3 cut(s) 176, 346, 360
Hpy99I CGWCG 2 cut(s) 61, 70
HpyAV CCTTC 1 cut(s) 355
HpyCH4IV ACGT 3 cut(s) 42, 56, 372
HpyCH4V TGCA 3 cut(s) 186, 227, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 427
HpyF3I CTNAG 3 cut(s) 35, 343, 471
HpySE526I ACGT 3 cut(s) 42, 56, 372
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 3 cut(s) 166, 425, 464
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 1 cut(s) 394
LpnPI CCDG 6 cut(s) 183, 231, 245, 275, 281, 302
Lsp1109I GCAGC 3 cut(s) 193, 206, 295
LweI GCATC 1 cut(s) 444
MaeI CTAG 2 cut(s) 135, 305
MaeII ACGT 3 cut(s) 42, 56, 372
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 2 cut(s) 16, 359
MfeI CAATTG 1 cut(s) 299
MluCI AATT 5 cut(s) 107, 125, 187, 299, 331
MnlI CCTC 5 cut(s) 24, 194, 352, 458, 472
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 290
MunI CAATTG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 203, 427
NdeII GATC 1 cut(s) 394
NheI GCTAGC 1 cut(s) 304
NlaIII CATG 3 cut(s) 166, 425, 464
NspI RCATGY 2 cut(s) 166, 425
PaeI GCATGC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 65
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 3 cut(s) 256, 279, 350
PflFI GACNNNGTC 2 cut(s) 58, 407
PkrI GCNGC 4 cut(s) 196, 208, 310, 434
Ppu21I YACGTR 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspPI GGNCC 1 cut(s) 467
PspPPI RGGWCCY 1 cut(s) 467
PsyI GACNNNGTC 2 cut(s) 58, 407
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 4 cut(s) 195, 207, 309, 433
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 290
SfaNI GCATC 1 cut(s) 444
SinI GGWCC 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 166
Sse9I AATT 5 cut(s) 107, 125, 187, 299, 331
SsiI CCGC 1 cut(s) 433
SspMI CTAG 2 cut(s) 135, 305
StyD4I CCNGG 1 cut(s) 288
TaiI ACGT 3 cut(s) 45, 59, 375
TaqI TCGA 2 cut(s) 68, 438
TasI AATT 5 cut(s) 107, 125, 187, 299, 331
TatI WGTACW 1 cut(s) 454
TauI GCSGC 1 cut(s) 435
TfiI GAWTC 3 cut(s) 256, 279, 350
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TseI GCWGC 3 cut(s) 194, 206, 308
TspDTI ATGAA 3 cut(s) 17, 120, 237
Tth111I GACNNNGTC 2 cut(s) 58, 407
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 1 cut(s) 125
XceI RCATGY 2 cut(s) 166, 425
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 135, 305
ZraI GACGTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.