Rh2AG230600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018104
Physical Location & Seq
Reverse (-)
24714579 .. 24719086
4508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG230600.1

Sequence Viewer

Length: 960 bp
ATGAAATTCGCTGATAGATATCAGCAATTTATGAAAGAGCAAAAAGAAGAAGTACCAGTCATCCGACTAAGCAATCTCAAGGCTGTTTTGAAGAACTGTGAGATCAAGGACATGCACCCCAAAGATAGAAATACTGAAGAAGACTGTGATTGTCCAGTATGTGACAGGACATTTTTCCCTTCCATTCTCAAGGACATGTCTGTGGTAGTGAACTTCTTTAACAAGCGTGCACAGGAGTTACTATACTCCCATTTGGCTTCTGGCTTTAAGAAGTACATTTTACATCTTAAAAGGAAGTTAAAGTTGAAAGCAAGCCATGAAAATATGATAAAAGAAGCGCAAGACCTTGTAGGCTACGCTACAATTACTGCTTTTGCTGTACAGAAGATCCTGAAGAAATATGATAAGGTTCACTGCTCGAAGAAGGGACAAAGGTTTAAATATCAAGCTCAAACTATGAAACTTGAGCTCCTAAAATCCCCATGGCTTTTGGAACTAATGGCATTACACATTAACGTGAGTAAAAACAGAGATGAACGCAAGACGATTTGGTTTGTTGACTTCTCACTTCACAAAGATAATGAGCACAGACCACTGTTTTTGGTTAATCTTCCCCACTCATTAGAGCTGAATCTTGATCTAACATGCCCAATTTGCTTGGACACTGTCTTTGATCCAGTTTTCCTCACCTGCAGCCATATACTCTGCCATATGTGTGCTTGTTCGGTTGCATCAGTCACCATTGTTGAGGGACTACGGGCTGCAACTTCTGCAAAGAAATGCCCAATTTGCCGAAAGGGTGGGGTATTTGGAGGCGCAGTGCGGTTGGCTGAGCTAAGCATGCTACTGAGTCGAAGTATGCCAAAGTATTGGAAAGGACGCCTTCAGAGGGAACGGCAAGAGCGAATCAAACAAGCAAAGGAGTTCTGGGAGTCCGAGTGTCGTTCATTTCTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPX zf-C3HC4_3 zf-C3HC4_4 zf-RING_UBOX

Protein Analysis

319

Amino Acids

37.14

Weight (kDa)

9.28

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018104)

Species Orthologous Gene IDs
malus_domestica MD03G1286600.v1.1
rosa_chinensis RchiOBHm_Chr2g0112121
rosa_laevigata RLG00000017953
rosa_multiflora Rmu_sc0000360.1_g000017 Rmu_sc0001294.1_g000020
rosa_roxburghii Rroxscaffold_2G00131750
rosa_rugosa Rorug02G0178500
rosa_samantha Rh2AG230600 Rh2BG243800 Rh2CG235200 Rh2DG238500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 698
Acc36I ACCTGC 1 cut(s) 698
AciI CCGC 1 cut(s) 823
AclWI GGATC 2 cut(s) 382, 668
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 3 cut(s) 156, 413, 869
AcyI GRCGYC 1 cut(s) 880
AfaI GTAC 3 cut(s) 54, 275, 381
AfiI CCNNNNNNNGG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 2 cut(s) 91, 307
AluBI AGCT 4 cut(s) 449, 469, 628, 835
AluI AGCT 4 cut(s) 449, 469, 628, 835
Alw21I GWGCWC 3 cut(s) 232, 471, 588
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 2 cut(s) 382, 668
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 693, 761
ApoI RAATTY 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AspLEI GCGC 2 cut(s) 340, 818
AsuHPI GGTGA 2 cut(s) 679, 730
BaeGI GKGCMC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 3 cut(s) 232, 471, 588
BbvI GCAGC 2 cut(s) 705, 748
BceAI ACGGC 1 cut(s) 911
BfmI CTRYAG 1 cut(s) 691
BfuAI ACCTGC 1 cut(s) 698
BisI GCNGC 2 cut(s) 694, 762
BlpI GCTNAGC 2 cut(s) 831, 836
BlsI GCNGC 2 cut(s) 695, 763
BmsI GCATC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 147
Bpu1102I GCTNAGC 2 cut(s) 831, 836
BpuEI CTTGAG 3 cut(s) 62, 173, 485
BsaBI GATNNNNATC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 1 cut(s) 482
BsaXI ACNNNNNCTCC 6 cut(s) 453, 483, 804, 834, 923, 953
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse1I ACTGG 3 cut(s) 56, 155, 677
Bse8I GATNNNNATC 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 1 cut(s) 60
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMII CTCAG 2 cut(s) 822, 839
BseNI ACTGG 3 cut(s) 56, 155, 677
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 2 cut(s) 705, 748
BsiHKAI GWGCWC 3 cut(s) 232, 471, 588
BslFI GGGAC 2 cut(s) 441, 765
BslI CCNNNNNNNGG 1 cut(s) 889
BsmFI GGGAC 2 cut(s) 441, 765
Bsp1286I GDGCHC 3 cut(s) 232, 471, 588
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 4 cut(s) 102, 387, 637, 673
Bsp1720I GCTNAGC 2 cut(s) 831, 836
Bsp19I CCATGG 1 cut(s) 482
BspACI CCGC 1 cut(s) 823
BspCNI CTCAG 2 cut(s) 823, 840
BspMAI CTGCAG 1 cut(s) 695
BspMI ACCTGC 1 cut(s) 698
BspPI GGATC 2 cut(s) 382, 668
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 3 cut(s) 56, 155, 677
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 4 cut(s) 102, 387, 637, 673
BssNI GRCGYC 1 cut(s) 880
BssT1I CCWWGG 1 cut(s) 482
Bst4CI ACNGT 4 cut(s) 98, 146, 597, 667
BstACI GRCGYC 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 379
BstC8I GCNNGC 3 cut(s) 228, 313, 842
BstDEI CTNAG 4 cut(s) 68, 831, 836, 848
BstDSI CCRYGG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 340, 818
BstKTI GATC 4 cut(s) 105, 390, 640, 676
BstMBI GATC 4 cut(s) 102, 387, 637, 673
BstMWI GCNNNNNNNGC 4 cut(s) 654, 770, 789, 841
BstNSI RCATGY 4 cut(s) 115, 199, 648, 844
BstSFI CTRYAG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 705, 748
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 387
BstXI CCANNNNNNTGG 1 cut(s) 870
BstYI RGATCY 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 482
BtsCI GGATG 1 cut(s) 60
BtsI GCAGTG 2 cut(s) 412, 825
BtsIMutI CAGTG 4 cut(s) 412, 593, 663, 825
BveI ACCTGC 1 cut(s) 698
Cac8I GCNNGC 3 cut(s) 228, 313, 842
CfoI GCGC 2 cut(s) 340, 818
CseI GACGC 1 cut(s) 888
Csp6I GTAC 3 cut(s) 53, 274, 380
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 6 cut(s) 112, 196, 317, 483, 645, 841
CviQI GTAC 3 cut(s) 53, 274, 380
DdeI CTNAG 4 cut(s) 68, 831, 836, 848
DpnI GATC 4 cut(s) 104, 389, 639, 675
DpnII GATC 4 cut(s) 102, 387, 637, 673
DraI TTTAAA 1 cut(s) 439
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 482
Eco24I GRGCYC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 3 cut(s) 156, 413, 869
EcoICRI GAGCTC 1 cut(s) 469
EcoRV GATATC 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 6 cut(s) 115, 199, 320, 486, 648, 844
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 2 cut(s) 441, 765
FatI CATG 6 cut(s) 111, 195, 316, 482, 644, 840
FauNDI CATATG 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 694, 762
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 2 cut(s) 694, 762
GlaI GCGC 2 cut(s) 339, 817
GluI GCNGC 2 cut(s) 694, 762
HgaI GACGC 1 cut(s) 888
HhaI GCGC 2 cut(s) 340, 818
Hin1I GRCGYC 1 cut(s) 880
Hin1II CATG 6 cut(s) 115, 199, 320, 486, 648, 844
Hin6I GCGC 2 cut(s) 338, 816
HinP1I GCGC 2 cut(s) 338, 816
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 4 cut(s) 631, 850, 906, 932
HphI GGTGA 2 cut(s) 679, 730
Hpy166II GTNNAC 4 cut(s) 211, 230, 412, 559
Hpy188I TCNGA 3 cut(s) 65, 888, 937
Hpy188III TCNNGA 3 cut(s) 391, 635, 953
Hpy8I GTNNAC 4 cut(s) 211, 230, 412, 559
HpyAV CCTTC 3 cut(s) 189, 418, 893
HpyCH4III ACNGT 4 cut(s) 98, 146, 597, 667
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 6 cut(s) 115, 230, 693, 731, 764, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 654, 770, 789, 841
HpyF3I CTNAG 4 cut(s) 68, 831, 836, 848
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 880
Hsp92II CATG 6 cut(s) 115, 199, 320, 486, 648, 844
HspAI GCGC 2 cut(s) 338, 816
Kzo9I GATC 4 cut(s) 102, 387, 637, 673
LmnI GCTCC 1 cut(s) 474
Lsp1109I GCAGC 2 cut(s) 705, 748
LweI GCATC 1 cut(s) 740
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 161, 237, 736
MalI GATC 4 cut(s) 104, 389, 639, 675
MboI GATC 4 cut(s) 102, 387, 637, 673
MboII GAAGA 8 cut(s) 59, 103, 149, 152, 397, 406, 433, 602
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 3 cut(s) 232, 471, 588
MluCI AATT 5 cut(s) 5, 26, 363, 651, 786
MlyI GAGTC 2 cut(s) 859, 941
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 4 cut(s) 695, 742, 806, 882
MseI TTAA 7 cut(s) 219, 267, 288, 299, 438, 513, 606
MslI CAYNNNNRTG 3 cut(s) 200, 515, 714
MwoI GCNNNNNNNGC 4 cut(s) 654, 770, 789, 841
NcoI CCATGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 711
NdeII GATC 4 cut(s) 102, 387, 637, 673
NlaIII CATG 6 cut(s) 115, 199, 320, 486, 648, 844
NmuCI GTSAC 2 cut(s) 161, 736
NspI RCATGY 4 cut(s) 115, 199, 648, 844
PaeI GCATGC 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 698
PciI ACATGT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 901
PfeI GAWTC 2 cut(s) 631, 906
PflFI GACNNNGTC 1 cut(s) 665
PkrI GCNGC 2 cut(s) 695, 763
PleI GAGTC 2 cut(s) 858, 940
PpsI GAGTC 2 cut(s) 858, 940
PscI ACATGT 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 471
PstI CTGCAG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 387
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 3 cut(s) 54, 275, 381
RsaNI GTAC 3 cut(s) 53, 274, 380
RseI CAYNNNNRTG 3 cut(s) 200, 515, 714
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 7 cut(s) 219, 267, 288, 299, 438, 513, 606
SatI GCNGC 2 cut(s) 694, 762
Sau3AI GATC 4 cut(s) 102, 387, 637, 673
SchI GAGTC 2 cut(s) 859, 941
SduI GDGCHC 3 cut(s) 232, 471, 588
SetI ASST 9 cut(s) 348, 411, 437, 451, 471, 519, 630, 692, 837
SfaNI GCATC 1 cut(s) 740
SfcI CTRYAG 1 cut(s) 691
SmiMI CAYNNNNRTG 3 cut(s) 200, 515, 714
SmlI CTYRAG 3 cut(s) 77, 188, 464
SmoI CTYRAG 3 cut(s) 77, 188, 464
SphI GCATGC 1 cut(s) 844
Sse9I AATT 5 cut(s) 5, 26, 363, 651, 786
SsiI CCGC 1 cut(s) 823
SstI GAGCTC 1 cut(s) 471
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 4 cut(s) 98, 146, 597, 667
TaiI ACGT 1 cut(s) 519
TaqI TCGA 2 cut(s) 419, 853
TasI AATT 5 cut(s) 5, 26, 363, 651, 786
TatI WGTACW 2 cut(s) 273, 379
TfiI GAWTC 2 cut(s) 631, 906
Tru1I TTAA 7 cut(s) 219, 267, 288, 299, 438, 513, 606
Tru9I TTAA 7 cut(s) 219, 267, 288, 299, 438, 513, 606
TscAI CASTG 4 cut(s) 419, 600, 670, 825
TseFI GTSAC 2 cut(s) 161, 736
TseI GCWGC 2 cut(s) 693, 761
Tsp45I GTSAC 2 cut(s) 161, 736
TspDTI ATGAA 6 cut(s) 17, 47, 333, 473, 549, 936
TspRI CASTG 4 cut(s) 419, 600, 670, 825
Tth111I GACNNNGTC 1 cut(s) 665
VneI GTGCAC 1 cut(s) 228
XapI RAATTY 1 cut(s) 5
XceI RCATGY 4 cut(s) 115, 199, 648, 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.