Rh2CG235200

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018104
Physical Location & Seq
Reverse (-)
24192350 .. 24196721
4372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG235200.1

Sequence Viewer

Length: 765 bp
ATGTCTGTGGTAGTGAACTTCTTTAACAAGCGTGCACAGGAGTTACTATACTCCCATTTGGCTTCTGGCTTTAAGAAGTACATTTTACGTCTTAAAAGGAAGTTAAAGTTGAAAGCAAGCCATGAAAATATGATAAAAGAAGCGCAAGACCTTGTAGGCTACGCTACAATTACTGCTTTTGCTATACAGAAGATCCTAAAGAAATATGATAAGGTTCACTGCTCGAAGAAGGGACAAAGGTTTAAATATCAAGCTCAAACTATGAAACTTGAGCTCCTAAAATCCCCATGGCTTTTGGAACTAATGGCATTCCACATTAACGTGAGTAAAAACAGAGATGAACGCAAGGCGATTTGGTTTGTTGACTTCTCGCTTCACAAAGATGATGAGCACAGACCACTGTTTTTGGTTAACCTTCCCCACTCAATAGAGCTGAATCTTGATCTAACATGCCCAATTTGCTTGGACACAGTCTTTGATCCAGTTTTCCTCACCTGCAGCCATATACTCTGCCATATGTGTGCTTGTTCGGTTGCATCAGTCACCATTGTTGAGGGACTACGGGCTGCAACTTCTGCAAAGAAATGCCCAATTTGCCGAAAGGGTGGGGTATTTGGAGGCGCAGTGCAGTTGGCTGAGCTAAGCATGCTACTGAGCCGAAGTATGCCAAAGTATTGGAAAGGACGCCTTCAGAGGGAACGGCAAGAGCGAATCAAACAAGCAAAGGAGTTCTGGGAGTCTGAGTGTCGTTCATTTCTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

254

Amino Acids

29.4

Weight (kDa)

9.52

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_XB3-XBAT31 PF24921 150 - 202 8.6e-06 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018104)

Species Orthologous Gene IDs
malus_domestica MD03G1286600.v1.1
rosa_chinensis RchiOBHm_Chr2g0112121
rosa_laevigata RLG00000017953
rosa_multiflora Rmu_sc0000360.1_g000017 Rmu_sc0001294.1_g000020
rosa_roxburghii Rroxscaffold_2G00131750
rosa_rugosa Rorug02G0178500
rosa_samantha Rh2AG230600 Rh2BG243800 Rh2CG235200 Rh2DG238500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 503
AclWI GGATC 2 cut(s) 187, 473
AcuI CTGAAG 1 cut(s) 674
AcyI GRCGYC 1 cut(s) 685
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 694
AgsI TTSAA 1 cut(s) 112
AluBI AGCT 4 cut(s) 254, 274, 433, 640
AluI AGCT 4 cut(s) 254, 274, 433, 640
Alw21I GWGCWC 3 cut(s) 37, 276, 393
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 2 cut(s) 187, 473
ApaLI GTGCAC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 498, 566
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 2 cut(s) 145, 623
AsuHPI GGTGA 2 cut(s) 484, 535
BaeGI GKGCMC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 3 cut(s) 37, 276, 393
BbvI GCAGC 2 cut(s) 510, 553
BceAI ACGGC 1 cut(s) 716
BfmI CTRYAG 1 cut(s) 496
BfuAI ACCTGC 1 cut(s) 503
BisI GCNGC 2 cut(s) 499, 567
BlpI GCTNAGC 2 cut(s) 636, 641
BlsI GCNGC 2 cut(s) 500, 568
BmsI GCATC 1 cut(s) 545
Bpu1102I GCTNAGC 2 cut(s) 636, 641
BpuEI CTTGAG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 287
BsaXI ACNNNNNCTCC 6 cut(s) 258, 288, 609, 639, 728, 758
Bsc4I CCNNNNNNNGG 1 cut(s) 694
Bse1I ACTGG 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 694
BseMII CTCAG 3 cut(s) 627, 644, 732
BseNI ACTGG 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 510, 553
BsgI GTGCAG 1 cut(s) 647
BsiHKAI GWGCWC 3 cut(s) 37, 276, 393
BslFI GGGAC 2 cut(s) 246, 570
BslI CCNNNNNNNGG 1 cut(s) 694
BsmFI GGGAC 2 cut(s) 246, 570
BsmI GAATGC 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 37, 276, 393
Bsp143I GATC 3 cut(s) 192, 442, 478
Bsp1720I GCTNAGC 2 cut(s) 636, 641
Bsp19I CCATGG 1 cut(s) 287
BspCNI CTCAG 3 cut(s) 628, 645, 733
BspMAI CTGCAG 1 cut(s) 500
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 2 cut(s) 187, 473
BsrI ACTGG 1 cut(s) 482
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 3 cut(s) 192, 442, 478
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 402, 472
BstACI GRCGYC 1 cut(s) 685
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 33, 118, 647
BstDEI CTNAG 4 cut(s) 636, 641, 653, 741
BstDSI CCRYGG 1 cut(s) 287
BstHHI GCGC 2 cut(s) 145, 623
BstKTI GATC 3 cut(s) 195, 445, 481
BstMBI GATC 3 cut(s) 192, 442, 478
BstMWI GCNNNNNNNGC 4 cut(s) 459, 575, 594, 646
BstNSI RCATGY 2 cut(s) 453, 649
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 510, 553
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 675
BstYI RGATCY 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 287
BtsI GCAGTG 2 cut(s) 217, 630
BtsIMutI CAGTG 3 cut(s) 217, 398, 630
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 3 cut(s) 33, 118, 647
CfoI GCGC 2 cut(s) 145, 623
CseI GACGC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 79
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 4 cut(s) 122, 288, 450, 646
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 4 cut(s) 636, 641, 653, 741
DpnI GATC 3 cut(s) 194, 444, 480
DpnII GATC 3 cut(s) 192, 442, 478
DraI TTTAAA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 674
EcoICRI GAGCTC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 4 cut(s) 125, 291, 453, 649
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 2 cut(s) 246, 570
FatI CATG 4 cut(s) 121, 287, 449, 645
FauNDI CATATG 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 499, 567
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 499, 567
GlaI GCGC 2 cut(s) 144, 622
GluI GCNGC 2 cut(s) 499, 567
HgaI GACGC 1 cut(s) 693
HhaI GCGC 2 cut(s) 145, 623
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 4 cut(s) 125, 291, 453, 649
Hin6I GCGC 2 cut(s) 143, 621
HinP1I GCGC 2 cut(s) 143, 621
HincII GTYRAC 2 cut(s) 364, 412
HindII GTYRAC 2 cut(s) 364, 412
HinfI GANTC 3 cut(s) 436, 711, 737
HpaI GTTAAC 1 cut(s) 412
HphI GGTGA 2 cut(s) 484, 535
Hpy166II GTNNAC 5 cut(s) 16, 35, 217, 364, 412
Hpy188I TCNGA 2 cut(s) 693, 742
Hpy188III TCNNGA 2 cut(s) 440, 758
Hpy8I GTNNAC 5 cut(s) 16, 35, 217, 364, 412
HpyAV CCTTC 3 cut(s) 223, 425, 698
HpyCH4III ACNGT 2 cut(s) 402, 472
HpyCH4IV ACGT 2 cut(s) 88, 321
HpyCH4V TGCA 6 cut(s) 35, 498, 536, 569, 578, 628
HpyF10VI GCNNNNNNNGC 4 cut(s) 459, 575, 594, 646
HpyF3I CTNAG 4 cut(s) 636, 641, 653, 741
HpySE526I ACGT 2 cut(s) 88, 321
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 4 cut(s) 125, 291, 453, 649
HspAI GCGC 2 cut(s) 143, 621
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 3 cut(s) 192, 442, 478
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 6 cut(s) 23, 51, 495, 508, 718, 743
Lsp1109I GCAGC 2 cut(s) 510, 553
LweI GCATC 1 cut(s) 545
MaeII ACGT 2 cut(s) 88, 321
MaeIII GTNAC 2 cut(s) 42, 541
MalI GATC 3 cut(s) 194, 444, 480
MboI GATC 3 cut(s) 192, 442, 478
MboII GAAGA 2 cut(s) 202, 238
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 3 cut(s) 37, 276, 393
MluCI AATT 3 cut(s) 168, 456, 591
MlyI GAGTC 1 cut(s) 746
MnlI CCTC 4 cut(s) 500, 547, 611, 687
MseI TTAA 7 cut(s) 24, 72, 93, 104, 243, 318, 411
MslI CAYNNNNRTG 3 cut(s) 320, 381, 519
Mva1269I GAATGC 1 cut(s) 308
MwoI GCNNNNNNNGC 4 cut(s) 459, 575, 594, 646
NcoI CCATGG 1 cut(s) 287
NdeI CATATG 1 cut(s) 516
NdeII GATC 3 cut(s) 192, 442, 478
NlaIII CATG 4 cut(s) 125, 291, 453, 649
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 2 cut(s) 453, 649
PaeI GCATGC 1 cut(s) 649
PaqCI CACCTGC 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 2 cut(s) 436, 711
PflFI GACNNNGTC 1 cut(s) 470
PkrI GCNGC 2 cut(s) 500, 568
PleI GAGTC 1 cut(s) 745
PpsI GAGTC 1 cut(s) 745
Psp124BI GAGCTC 1 cut(s) 276
PstI CTGCAG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 192
PsyI GACNNNGTC 1 cut(s) 470
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 3 cut(s) 320, 381, 519
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 7 cut(s) 24, 72, 93, 104, 243, 318, 411
SatI GCNGC 2 cut(s) 499, 567
Sau3AI GATC 3 cut(s) 192, 442, 478
SchI GAGTC 1 cut(s) 746
SduI GDGCHC 3 cut(s) 37, 276, 393
SfaNI GCATC 1 cut(s) 545
SfcI CTRYAG 1 cut(s) 496
SmiMI CAYNNNNRTG 3 cut(s) 320, 381, 519
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SphI GCATGC 1 cut(s) 649
Sse9I AATT 3 cut(s) 168, 456, 591
SstI GAGCTC 1 cut(s) 276
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 402, 472
TaiI ACGT 2 cut(s) 91, 324
TaqI TCGA 1 cut(s) 224
TasI AATT 3 cut(s) 168, 456, 591
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 2 cut(s) 436, 711
Tru1I TTAA 7 cut(s) 24, 72, 93, 104, 243, 318, 411
Tru9I TTAA 7 cut(s) 24, 72, 93, 104, 243, 318, 411
TscAI CASTG 3 cut(s) 224, 405, 630
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 2 cut(s) 498, 566
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 4 cut(s) 138, 278, 354, 741
TspRI CASTG 3 cut(s) 224, 405, 630
Tth111I GACNNNGTC 1 cut(s) 470
VneI GTGCAC 1 cut(s) 33
XceI RCATGY 2 cut(s) 453, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.