Rh2AG559100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013453
Physical Location & Seq
Reverse (-)
79005444 .. 79006373
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG559100.1

Sequence Viewer

Length: 930 bp
ATGATTTCGGTTTGTACCCGCAATGCTCATGTTGCTGAAGCTTTGAGGTTGTTTGAGGCTATGGATGTTGAGCCTGATGTGTCGACTTATAACACCATCATAGCCGGAACGTCGGGGATGGATGAGGGGTTGTTTAGGGCTGTGGAGGTGTATAGGAAAATGTGCGAAAGAGGGTGGAAGCCGGATTTGATCACACTTCTTGCTCTGTTACGTGCTTGTGTTGGTGTAGCAGCTTTTAGGTGTATTAAGGAAATTCATGGTTATGATGTAAGGAATGGGATTGACTCGCATTCGCAGTTGAGTAGCGGTTTAGTAGAGGCCTATGGGCGTTGTGGTTGTCTCGAAAATGCGCATAGTGTTTTCCGGGGCTTGAAGGAGAAGGATGTGGTTGCGTGGAGCAGTTTGATATCAGTGTATGCGGTTCATGGGGAGGCAAGAGCTGCATTTGATGTTTTTAGAGGAATGGAATTGGAGAAAGTGGAGCCTGATGAGATTACTTTTCTTGCGGTACTGAAAGCGTGTAGCCATGCCGAAGGATTAGCTGATGAAGCACTGAGTTATTTTGATTGTATGCGTAATTATGGCGTGCAACCAAACAGTGATCACTATTCTTGTTTAGTAGATGTTTTGAGCAGAGCAGGGAGGTTATGCGAGGCGTATGAGGTCATAAGGAAGATGCCAGTGAAGGTGACTGCTAGAGCTTGGGGTGCGCTTCTTTCGGGGTGTAGAACTTATGGAAACCTGGAGCTCGCGGAGATAGCAGGGAAAGCTTTGTCCGAACTTGAGCCCGATAATCCTTCCCATTATTTATTGTTGTCAACGATTTATGTTAGCTTAGGAAGGCATGATGAAGCTCAGAGAATGAGAAGGGAAATGAGGCAGAAAGGTGTGAAGGCACCGGTTGGAAGTAGTTGGATGGTTCAAAAGTGA

Protein Analysis

309

Amino Acids

34.26

Weight (kDa)

6.16

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 25 - 74 3.1e-07 PPR repeat family
PPR_2 PF13041 127 - 175 1.7e-09 PPR repeat family
PPR_3 PF13812 127 - 174 1e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 137 - 248 1.3e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 143 - 240 3.3e-08 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 245 - 306 5e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013453)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03510
fragaria_vesca FvH4_6g44770
malus_domestica MD09G1093700.v1.1 MD17G1083400.v1.1
prunus_persica Prupe.3G231700_v2.0.a1
pyrus_communis pycom09g01890 pycom17g08120
rosa_chinensis RchiOBHm_Chr2g0161921
rosa_laevigata RLG00000021307
rosa_multiflora Rmu_sc0008565.1_g000004 Rmu_sc0018381.1_g000006
rosa_roxburghii Rroxscaffold_2G00088460
rosa_rugosa Rorug02G0493200
rosa_samantha Rh2AG559100 Rh2BG572500 Rh2CG542800 Rh2DG582300
rosa_wichuraiana Rw2G046280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 90
Acc16I TGCGCA 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 895
AccI GTMKAC 1 cut(s) 83
AccII CGCG 1 cut(s) 752
AciI CCGC 5 cut(s) 19, 306, 419, 506, 752
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 2 cut(s) 16, 510
AgeI ACCGGT 1 cut(s) 898
AgsI TTSAA 2 cut(s) 373, 923
AjnI CCWGG 1 cut(s) 741
AluBI AGCT 9 cut(s) 41, 233, 440, 542, 701, 748, 770, 834, 854
AluI AGCT 9 cut(s) 41, 233, 440, 542, 701, 748, 770, 834, 854
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 1 cut(s) 344
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 2 cut(s) 230, 440
ApoI RAATTY 1 cut(s) 252
AsiGI ACCGGT 1 cut(s) 898
AspLEI GCGC 2 cut(s) 352, 712
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 700
BanI GGYRCC 1 cut(s) 895
BanII GRGCYC 2 cut(s) 750, 789
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 2 cut(s) 242, 427
BccI CCATC 3 cut(s) 104, 112, 910
BciT130I CCWGG 1 cut(s) 743
BclI TGATCA 2 cut(s) 189, 601
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 1 cut(s) 696
BisI GCNGC 2 cut(s) 231, 441
BlsI GCNGC 2 cut(s) 232, 442
Bme1390I CCNGG 2 cut(s) 365, 743
BmiI GGNNCC 2 cut(s) 483, 897
BmrFI CCNGG 2 cut(s) 365, 743
BmsI GCATC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 835
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 364
BsaWI WCCGGW 1 cut(s) 898
Bse118I RCCGGY 1 cut(s) 898
Bse1I ACTGG 1 cut(s) 680
Bse3DI GCAATG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 743
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 5 cut(s) 70, 123, 127, 388, 921
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 545, 869
BseNI ACTGG 1 cut(s) 680
BseXI GCAGC 2 cut(s) 242, 427
Bsh1236I CGCG 1 cut(s) 752
BshFI GGCC 1 cut(s) 320
BshNI GGYRCC 1 cut(s) 895
BshTI ACCGGT 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 750
BsiSI CCGG 4 cut(s) 105, 182, 364, 899
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 750, 789
Bsp143I GATC 2 cut(s) 189, 601
BspACI CCGC 5 cut(s) 19, 306, 419, 506, 752
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 546, 868
BspFNI CGCG 1 cut(s) 752
BspLI GGNNCC 2 cut(s) 483, 897
BspT107I GGYRCC 1 cut(s) 895
BsrDI GCAATG 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 898
BsrI ACTGG 1 cut(s) 680
BssAI RCCGGY 1 cut(s) 898
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 2 cut(s) 189, 601
Bst2UI CCWGG 1 cut(s) 743
Bst4CI ACNGT 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 587, 750
BstDEI CTNAG 3 cut(s) 554, 835, 855
BstF5I GGATG 5 cut(s) 70, 123, 127, 388, 921
BstFNI CGCG 1 cut(s) 752
BstHHI GCGC 2 cut(s) 352, 712
BstKTI GATC 2 cut(s) 192, 604
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 189, 601
BstMWI GCNNNNNNNGC 6 cut(s) 32, 440, 548, 707, 758, 767
BstNI CCWGG 1 cut(s) 743
BstSCI CCNGG 2 cut(s) 363, 741
BstUI CGCG 1 cut(s) 752
BstV1I GCAGC 2 cut(s) 242, 427
BsuRI GGCC 1 cut(s) 320
BtsCI GGATG 5 cut(s) 70, 123, 127, 388, 921
BtsIMutI CAGTG 4 cut(s) 417, 551, 604, 687
Cac8I GCNNGC 2 cut(s) 587, 750
CfoI GCGC 2 cut(s) 352, 712
Cfr10I RCCGGY 1 cut(s) 898
Csp6I GTAC 2 cut(s) 15, 509
CspAI ACCGGT 1 cut(s) 898
CviAII CATG 5 cut(s) 29, 257, 425, 527, 845
CviQI GTAC 2 cut(s) 15, 509
DdeI CTNAG 3 cut(s) 554, 835, 855
DpnI GATC 2 cut(s) 191, 603
DpnII GATC 2 cut(s) 189, 601
Ecl136II GAGCTC 1 cut(s) 748
Eco147I AGGCCT 1 cut(s) 320
Eco24I GRGCYC 2 cut(s) 750, 789
Eco32I GATATC 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 748
EcoRII CCWGG 1 cut(s) 741
EcoRV GATATC 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 750, 789
FaeI CATG 5 cut(s) 32, 260, 428, 530, 848
FatI CATG 5 cut(s) 28, 256, 424, 526, 844
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 2 cut(s) 189, 601
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 2 cut(s) 231, 441
FokI GGATG 4 cut(s) 77, 130, 134, 395
FriOI GRGCYC 2 cut(s) 750, 789
Fsp4HI GCNGC 2 cut(s) 231, 441
FspAI RTGCGCAY 1 cut(s) 351
FspBI CTAG 1 cut(s) 696
FspI TGCGCA 1 cut(s) 351
GlaI GCGC 2 cut(s) 351, 711
GluI GCNGC 2 cut(s) 231, 441
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 1 cut(s) 320
HapII CCGG 4 cut(s) 105, 182, 364, 899
HhaI GCGC 2 cut(s) 352, 712
Hin1II CATG 5 cut(s) 32, 260, 428, 530, 848
Hin6I GCGC 2 cut(s) 350, 710
HinP1I GCGC 2 cut(s) 350, 710
HincII GTYRAC 2 cut(s) 84, 819
HindII GTYRAC 2 cut(s) 84, 819
HindIII AAGCTT 2 cut(s) 39, 768
HinfI GANTC 1 cut(s) 284
HpaII CCGG 4 cut(s) 105, 182, 364, 899
HphI GGTGA 1 cut(s) 700
Hpy166II GTNNAC 2 cut(s) 84, 819
Hpy188I TCNGA 2 cut(s) 778, 858
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 2 cut(s) 84, 819
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 8 cut(s) 367, 373, 527, 679, 807, 834, 861, 886
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 2 cut(s) 110, 211
HpyCH4V TGCA 2 cut(s) 443, 589
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 440, 548, 707, 758, 767
HpyF3I CTNAG 3 cut(s) 554, 835, 855
HpySE526I ACGT 2 cut(s) 110, 211
Hsp92II CATG 5 cut(s) 32, 260, 428, 530, 848
HspAI GCGC 2 cut(s) 350, 710
Ksp22I TGATCA 2 cut(s) 189, 601
Kzo9I GATC 2 cut(s) 189, 601
LmnI GCTCC 3 cut(s) 396, 481, 745
Lsp1109I GCAGC 2 cut(s) 242, 427
LweI GCATC 1 cut(s) 666
MaeI CTAG 1 cut(s) 696
MaeII ACGT 2 cut(s) 110, 211
MaeIII GTNAC 2 cut(s) 207, 688
MalI GATC 2 cut(s) 191, 603
MboI GATC 2 cut(s) 189, 601
MboII GAAGA 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 750, 789
MluCI AATT 3 cut(s) 252, 467, 577
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 2 cut(s) 883, 893
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 261
MspI CCGG 4 cut(s) 105, 182, 364, 899
MspR9I CCNGG 2 cut(s) 365, 743
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 752
MwoI GCNNNNNNNGC 6 cut(s) 32, 440, 548, 707, 758, 767
NciI CCSGG 1 cut(s) 365
NdeII GATC 2 cut(s) 189, 601
NlaIII CATG 5 cut(s) 32, 260, 428, 530, 848
NlaIV GGNNCC 2 cut(s) 483, 897
NmuCI GTSAC 1 cut(s) 688
NsbI TGCGCA 1 cut(s) 351
PceI AGGCCT 1 cut(s) 320
PctI GAATGC 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 898
PkrI GCNGC 2 cut(s) 232, 442
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 212
PsiI TTATAA 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 741
PspGI CCWGG 1 cut(s) 741
PspN4I GGNNCC 2 cut(s) 483, 897
RsaI GTAC 2 cut(s) 16, 510
RsaNI GTAC 2 cut(s) 15, 509
RseI CAYNNNNRTG 1 cut(s) 261
SacI GAGCTC 1 cut(s) 750
SalI GTCGAC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 2 cut(s) 231, 441
Sau3AI GATC 2 cut(s) 189, 601
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 365, 743
SduI GDGCHC 2 cut(s) 750, 789
SfaNI GCATC 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 3 cut(s) 252, 467, 577
SseBI AGGCCT 1 cut(s) 320
SsiI CCGC 5 cut(s) 19, 306, 419, 506, 752
SspMI CTAG 1 cut(s) 696
SstI GAGCTC 1 cut(s) 750
StuI AGGCCT 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 363, 741
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 2 cut(s) 113, 214
TaqI TCGA 2 cut(s) 83, 342
TasI AATT 3 cut(s) 252, 467, 577
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 4 cut(s) 417, 558, 604, 687
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 2 cut(s) 230, 440
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 245, 413, 561, 864
TspRI CASTG 4 cut(s) 417, 558, 604, 687
XapI RAATTY 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 83
XspI CTAG 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.