Rh2BG076600

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017423
Physical Location & Seq
Reverse (-)
5881503 .. 5892430
10928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG076600.1

Sequence Viewer

Length: 333 bp
ATGCTTCACAGGAGCTTCAAGCCCGCCAAATGCAAAACGGCGTTGAAGCTTGCTGTTTCGAGAATTAAGCTGTTGAAGAATAAGAAAGATGCCCAGGTGAAGCAGATAAAGAGGGAATTGGCCCAATTGCTGGAGAGTGGTCAGTACAGGACTGCAAGGATTCGGTTTCCTGATGCGGAAATTCGGAACATCATGCAAGAATTCGATCAACCTGGGACGCACAGGGTGTTGTCCTGTGGAGGGACTGACCATGTGGTAGTCCAATCCATTCCTCGTATTTTAATTCGCGCACAGCTGCCGCTTAGGCATCGGAAAGGAGTCGGAGGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

110

Amino Acids

12.52

Weight (kDa)

11.0

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 56 3.1e-12 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 130
AccII CGCG 1 cut(s) 288
AciI CCGC 3 cut(s) 24, 176, 299
AcsI RAATTY 2 cut(s) 180, 200
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 2 cut(s) 130, 240
AgsI TTSAA 3 cut(s) 19, 46, 76
AjnI CCWGG 2 cut(s) 93, 211
AluBI AGCT 4 cut(s) 15, 49, 70, 295
AluI AGCT 4 cut(s) 15, 49, 70, 295
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 2 cut(s) 180, 200
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 109
BbvI GCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 2 cut(s) 95, 213
BglI GCCNNNNNGGC 1 cut(s) 304
BisI GCNGC 2 cut(s) 296, 299
BlsI GCNGC 2 cut(s) 297, 300
Bme1390I CCNGG 2 cut(s) 95, 213
BmgT120I GGNCC 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 95, 213
BmsI GCATC 3 cut(s) 79, 163, 316
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 93, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 240
BseBI CCWGG 2 cut(s) 95, 213
BseDI CCNNGG 2 cut(s) 93, 212
BseLI CCNNNNNNNGG 2 cut(s) 130, 240
BseXI GCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 122
BslFI GGGAC 2 cut(s) 229, 256
BslI CCNNNNNNNGG 2 cut(s) 130, 240
BsmFI GGGAC 2 cut(s) 229, 256
BsnI GGCC 1 cut(s) 122
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 3 cut(s) 24, 176, 299
BspANI GGCC 1 cut(s) 122
BspFNI CGCG 1 cut(s) 288
BssECI CCNNGG 2 cut(s) 93, 212
BssMI GATC 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 95, 213
BstC8I GCNNGC 2 cut(s) 24, 51
BstDEI CTNAG 1 cut(s) 302
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNI CCWGG 2 cut(s) 95, 213
BstSCI CCNGG 2 cut(s) 93, 211
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 24, 51
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 145
CviAII CATG 2 cut(s) 193, 251
CviJI RGCY 6 cut(s) 15, 22, 49, 70, 122, 295
CviKI_1 RGCY 6 cut(s) 15, 22, 49, 70, 122, 295
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 302
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DraIII CACNNNGTG 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 93, 211
FaeI CATG 2 cut(s) 196, 254
FaiI YATR 2 cut(s) 194, 252
FaqI GGGAC 2 cut(s) 229, 256
FatI CATG 2 cut(s) 192, 250
FauI CCCGC 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 296, 299
Fsp4HI GCNGC 2 cut(s) 296, 299
GlaI GCGC 1 cut(s) 289
GluI GCNGC 2 cut(s) 296, 299
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 122
HgaI GACGC 1 cut(s) 226
HhaI GCGC 1 cut(s) 290
Hin1II CATG 2 cut(s) 196, 254
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 160, 318
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 186, 312, 323
Hpy188III TCNNGA 2 cut(s) 60, 170
HpyCH4V TGCA 3 cut(s) 33, 155, 196
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 302
Hsp92II CATG 2 cut(s) 196, 254
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 1 cut(s) 12
LpnPI CCDG 9 cut(s) 80, 107, 116, 133, 183, 198, 208, 225, 247
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 3 cut(s) 79, 163, 316
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 88
MfeI CAATTG 1 cut(s) 125
MluCI AATT 6 cut(s) 63, 116, 125, 180, 200, 282
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 4 cut(s) 105, 233, 282, 317
MseI TTAA 2 cut(s) 66, 281
MspA1I CMGCKG 1 cut(s) 295
MspR9I CCNGG 2 cut(s) 95, 213
MunI CAATTG 1 cut(s) 125
MvaI CCWGG 2 cut(s) 95, 213
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 304
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 196, 254
PfeI GAWTC 1 cut(s) 160
PflMI CCANNNNNTGG 1 cut(s) 130
PkrI GCNGC 2 cut(s) 297, 300
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 93, 211
PspGI CCWGG 2 cut(s) 93, 211
PspPI GGNCC 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 66, 281
SatI GCNGC 2 cut(s) 296, 299
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 2 cut(s) 95, 213
SetI ASST 6 cut(s) 17, 51, 72, 99, 214, 297
SfaNI GCATC 3 cut(s) 79, 163, 316
Sse9I AATT 6 cut(s) 63, 116, 125, 180, 200, 282
SsiI CCGC 3 cut(s) 24, 176, 299
StyD4I CCNGG 2 cut(s) 93, 211
TaqI TCGA 2 cut(s) 59, 204
TasI AATT 6 cut(s) 63, 116, 125, 180, 200, 282
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 66, 281
Tru9I TTAA 2 cut(s) 66, 281
TseI GCWGC 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 130
XapI RAATTY 2 cut(s) 180, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.