Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0010328

Physical Location & Seq

Genomic Coordinates Forward (+)

76168767 .. 76170125

Gene Structure 1359 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG553400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGTTATTAATCTCTCATCTTCTCCACCCTCATCTTCTTTGTCTTTCATCAAGTTAACCAATTCAAGGTCCCCCATCTGTGAGTTTCCATCAAAACCCAAAACCCACCTCCCAAAGCCCCTATCTTGCTGCTCATTTTCTTCATCAGACCTTAACTTTCATTGCAAGGATCCATTTTTATCTGTAAAGGGTAGGAGCCCAAATGGGAGAAGGTTAAAGTTCAGGTCTTGTGCACTTCCTGGTTTTGATTATGGCAACTTTGAGAGTGTTCAGTCAGTTTTGGAGGCAGCTGGAGTGTTAACAGCCATCATTGTTGTTCATGAAAGTGGTCATTTCCTTGCTGCTTATCTTCAGGGTATCCATGTAAGTAAATTTGCAGTTGGGTTTGGTCCAATTTTAGCTAAATTCAATGCCAACAATGTAGAGTATTCCATCAGAGCTTTCCCTCTAGGAGGGTTTGTGGGGTTTCCTGATAATGATCCAAATAGTGATATCCCAGTTGATGATGTAAATTTGCTCAAGAATAGGCCAATATTGGATAGAGTAATAGTGGTTTCAGCTGGTGTGATTGCCAACGTAATTTTTGCCTATGCTATAATCTTTACTCAAGTCTTGTCAGTTGGTTTGCCTGTACAAGAGGGCTATCCTGGGGTTCTTGTGCCTGAGGTTCGACCCTTTTCGGCTGCTTCCCGAGACGGGTTGCTTGCAGGTGATGTAATCCTTGAGGTTAATGGAATTCAATTGCCGAAAGGAGGGTCTAATGCTGTTACAGGGGTTGTTGATGTGATTAAGAAAAATCCAAAAAGAAATGTGGTTCTTAAGGTTGAGAGGGGAAAGCAGGATTTCGAGATTGGAGTCACCCCCGATGAGAATTATGATGGAACAGGCAAAATTGGAGTGCAGTTATCACCAAATGTTAAGCTTTCGAAAGCTAAACCCAAAAATATATCGGAGGCTTTCAATTATACAGGGAGAGAGTTCTGGGGCTTGTCGTTTAATGTATTGGATAGCTTAAAACAGACTGTTCTAAACTTCTCGCAGACAGCTAGTAAAGTTTCGGGTCCAGTTGCCATCATTGCTGTTGGTGCAGAAGTTGCAAGGTCGAATCTTGATGGACTGTATCAATTTGCTGCTTTGCTTAATCTTAACCTTGCAGTGATCAACCTTCTTCCCTTACCTGCTCTAGATGGAGGTACCTTGGCTTTCATACTTATAGAGGCTGCTAGGGGTGGTAGAAAACTCCCTCTAGAAGTGGAACAGACGATTATGTCGTCAGGGATAACGGTAGTTGTTTTCCTAGGATTATTCCTTATTGTCCGGGACACACTTAATCTTGATTTTATCAGAGACATGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0006950 GO:0006996 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009628 GO:0009657 GO:0009658 GO:0009706 GO:0009941 GO:0009987 GO:0016020 GO:0016043 GO:0016787 GO:0019538 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0042170 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Peptidase_M50

Protein Analysis

452

Amino Acids

48.52

Weight (kDa)

8.67

Isoelectric Point (pI)

36.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50	PF02163	97 - 432	5.2e-57	Peptidase family M50
PDZ	PF00595	207 - 274	4.7e-06	PDZ domain
PDZ_2	PF13180	208 - 284	3e-07	PDZ domain
PDZ_6	PF17820	220 - 277	8.2e-08	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05140 AT2G32480 AT2G32480
fragaria_vesca	FvH4_6g43160 FvH4_6g43160
malus_domestica	MD09G1110000.v1.1
prunus_persica	Prupe.3G216500_v2.0.a1 Prupe.3G216500_v2.0.a1 Prupe.3G216500_v2.0.a1
pyrus_communis	pycom09g03250 pycom17g09350
rosa_chinensis	RchiOBHm_Chr2g0159081
rosa_laevigata	RLG00000021136
rosa_multiflora	Rmu_sc0003185.1_g000006
rosa_roxburghii	Rroxscaffold_2G00090800
rosa_rugosa	Rorug02G0473900
rosa_samantha	Rh2AG540700 Rh2BG553400 Rh2CG523800 Rh2DG563300
rosa_wichuraiana	Rw2G044740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	698
AasI	GACNNNNNNGTC	1 cut(s)	1267
Acc36I	ACCTGC	2 cut(s)	698, 1186
Acc65I	GGTACC	1 cut(s)	1193
AccB1I	GGYRCC	1 cut(s)	1193
AclWI	GGATC	3 cut(s)	164, 177, 473
AcsI	RAATTY	4 cut(s)	371, 404, 511, 735
AcuI	CTGAAG	1 cut(s)	335
AfaI	GTAC	2 cut(s)	633, 1195
AfiI	CCNNNNNNNGG	4 cut(s)	66, 452, 696, 752
AflII	CTTAAG	1 cut(s)	818
AflIII	ACRYGT	1 cut(s)	1350
AgsI	TTSAA	4 cut(s)	66, 409, 740, 961
AjnI	CCWGG	2 cut(s)	238, 646
AluBI	AGCT	8 cut(s)	290, 401, 440, 560, 922, 932, 1011, 1046
AluI	AGCT	8 cut(s)	290, 401, 440, 560, 922, 932, 1011, 1046
Alw21I	GWGCWC	1 cut(s)	235
Alw26I	GTCTC	2 cut(s)	687, 1341
Alw44I	GTGCAC	1 cut(s)	231
AlwI	GGATC	3 cut(s)	164, 177, 473
Ama87I	CYCGRG	1 cut(s)	690
AoxI	GGCC	1 cut(s)	527
ApaLI	GTGCAC	1 cut(s)	231
ApeKI	GCWGC	6 cut(s)	129, 287, 341, 683, 1130, 1220
ApoI	RAATTY	4 cut(s)	371, 404, 511, 735
ArsI	GACNNNNNNTTYG	2 cut(s)	740, 772
AseI	ATTAAT	1 cut(s)	9
Asp718I	GGTACC	1 cut(s)	1193
AspA2I	CCTAGG	1 cut(s)	1297
AspS9I	GGNCC	3 cut(s)	69, 389, 1061
AsuC2I	CCSGG	1 cut(s)	1319
AsuHPI	GGTGA	3 cut(s)	722, 850, 900
AsuII	TTCGAA	1 cut(s)	926
AvaI	CYCGRG	1 cut(s)	690
AvaII	GGWCC	3 cut(s)	69, 389, 1061
AvrII	CCTAGG	1 cut(s)	1297
AxyI	CCTNAGG	1 cut(s)	663
BaeGI	GKGCMC	1 cut(s)	235
BamHI	GGATCC	1 cut(s)	169
BanI	GGYRCC	1 cut(s)	1193
BanII	GRGCYC	1 cut(s)	200
Bbv12I	GWGCWC	1 cut(s)	235
BbvI	GCAGC	6 cut(s)	116, 299, 328, 670, 1117, 1207
BccI	CCATC	8 cut(s)	83, 97, 314, 440, 872, 1079, 1106, 1181
BciT130I	CCWGG	2 cut(s)	240, 648
BciVI	GTATCC	1 cut(s)	368
BclI	TGATCA	1 cut(s)	1158
BcnI	CCSGG	1 cut(s)	1319
BcoDI	GTCTC	2 cut(s)	687, 1341
BfaI	CTAG	6 cut(s)	449, 1047, 1184, 1224, 1247, 1298
BfrI	CTTAAG	1 cut(s)	818
BfuAI	ACCTGC	2 cut(s)	698, 1186
BfuI	GTATCC	1 cut(s)	368
BisI	GCNGC	6 cut(s)	130, 288, 342, 684, 1131, 1221
BlnI	CCTAGG	1 cut(s)	1297
BlsI	GCNGC	6 cut(s)	131, 289, 343, 685, 1132, 1222
Bme1390I	CCNGG	3 cut(s)	240, 648, 1319
Bme18I	GGWCC	3 cut(s)	69, 389, 1061
BmeT110I	CYCGRG	1 cut(s)	690
BmgT120I	GGNCC	3 cut(s)	69, 389, 1061
BmiI	GGNNCC	5 cut(s)	71, 171, 197, 1062, 1195
BmrFI	CCNGG	3 cut(s)	240, 648, 1319
BmrI	ACTGGG	1 cut(s)	491
BmuI	ACTGGG	1 cut(s)	491
BpmI	CTGGAG	1 cut(s)	312
Bpu14I	TTCGAA	1 cut(s)	926
BpuEI	CTTGAG	3 cut(s)	503, 591, 743
BpuMI	CCSGG	1 cut(s)	1319
BsaBI	GATNNNNATC	1 cut(s)	477
BsaJI	CCNNGG	3 cut(s)	647, 1197, 1297
BsaXI	ACNNNNNCTCC	2 cut(s)	444, 474
Bsc4I	CCNNNNNNNGG	4 cut(s)	66, 452, 696, 752
Bse1I	ACTGG	2 cut(s)	497, 1064
Bse21I	CCTNAGG	1 cut(s)	663
Bse3DI	GCAATG	2 cut(s)	160, 1074
Bse8I	GATNNNNATC	1 cut(s)	477
BseBI	CCWGG	2 cut(s)	240, 648
BseDI	CCNNGG	3 cut(s)	647, 1197, 1297
BseJI	GATNNNNATC	1 cut(s)	477
BseLI	CCNNNNNNNGG	4 cut(s)	66, 452, 696, 752
BseMI	GCAATG	2 cut(s)	160, 1074
BseMII	CTCAG	1 cut(s)	654
BseNI	ACTGG	2 cut(s)	497, 1064
BseSI	GKGCMC	1 cut(s)	235
BseXI	GCAGC	6 cut(s)	116, 299, 328, 670, 1117, 1207
BsgI	GTGCAG	2 cut(s)	920, 1107
BshFI	GGCC	1 cut(s)	529
BshNI	GGYRCC	1 cut(s)	1193
BsiHKAI	GWGCWC	1 cut(s)	235
BsiHKCI	CYCGRG	1 cut(s)	690
BsiSI	CCGG	1 cut(s)	1318
BslFI	GGGAC	2 cut(s)	55, 1334
BslI	CCNNNNNNNGG	4 cut(s)	66, 452, 696, 752
BsmAI	GTCTC	2 cut(s)	687, 1341
BsmBI	CGTCTC	1 cut(s)	687
BsmFI	GGGAC	2 cut(s)	55, 1334
BsnI	GGCC	1 cut(s)	529
BsoBI	CYCGRG	1 cut(s)	690
Bsp119I	TTCGAA	1 cut(s)	926
Bsp1286I	GDGCHC	2 cut(s)	200, 235
Bsp1407I	TGTACA	1 cut(s)	631
Bsp143I	GATC	3 cut(s)	169, 478, 1158
BspANI	GGCC	1 cut(s)	529
BspCNI	CTCAG	1 cut(s)	655
BspHI	TCATGA	1 cut(s)	319
BspLI	GGNNCC	5 cut(s)	71, 171, 197, 1062, 1195
BspMI	ACCTGC	2 cut(s)	698, 1186
BspPI	GGATC	3 cut(s)	164, 177, 473
BspT104I	TTCGAA	1 cut(s)	926
BspT107I	GGYRCC	1 cut(s)	1193
BspTI	CTTAAG	1 cut(s)	818
BsrDI	GCAATG	2 cut(s)	160, 1074
BsrGI	TGTACA	1 cut(s)	631
BsrI	ACTGG	2 cut(s)	497, 1064
BssECI	CCNNGG	3 cut(s)	647, 1197, 1297
BssMI	GATC	3 cut(s)	169, 478, 1158
BssT1I	CCWWGG	2 cut(s)	1197, 1297
Bst2UI	CCWGG	2 cut(s)	240, 648
Bst4CI	ACNGT	3 cut(s)	1024, 1119, 1285
BstAFI	CTTAAG	1 cut(s)	818
BstAPI	GCANNNNNTGC	1 cut(s)	1094
BstAUI	TGTACA	1 cut(s)	631
BstBI	TTCGAA	1 cut(s)	926
BstC8I	GCNNGC	1 cut(s)	705
BstDEI	CTNAG	1 cut(s)	663
BstENI	CCTNNNNNAGG	1 cut(s)	450
BstKTI	GATC	3 cut(s)	172, 481, 1161
BstMAI	GTCTC	2 cut(s)	687, 1341
BstMBI	GATC	3 cut(s)	169, 478, 1158
BstMWI	GCNNNNNNNGC	3 cut(s)	1076, 1085, 1094
BstNI	CCWGG	2 cut(s)	240, 648
BstNSI	RCATGY	1 cut(s)	1354
BstSCI	CCNGG	3 cut(s)	238, 646, 1317
BstSLI	GKGCMC	1 cut(s)	235
BstV1I	GCAGC	6 cut(s)	116, 299, 328, 670, 1117, 1207
BstX2I	RGATCY	1 cut(s)	169
BstYI	RGATCY	1 cut(s)	169
Bsu36I	CCTNAGG	1 cut(s)	663
BsuI	GTATCC	1 cut(s)	368
BsuRI	GGCC	1 cut(s)	529
BtsI	GCAGTG	1 cut(s)	1161
BtsIMutI	CAGTG	1 cut(s)	1161
BveI	ACCTGC	2 cut(s)	698, 1186
Cac8I	GCNNGC	1 cut(s)	705
CciI	TCATGA	1 cut(s)	319
Cfr13I	GGNCC	3 cut(s)	69, 389, 1061
Csp6I	GTAC	2 cut(s)	632, 1194
CviAII	CATG	3 cut(s)	320, 362, 1351
CviQI	GTAC	2 cut(s)	632, 1194
DdeI	CTNAG	1 cut(s)	663
DpnI	GATC	3 cut(s)	171, 480, 1160
DpnII	GATC	3 cut(s)	169, 478, 1158
DrdI	GACNNNNNNGTC	1 cut(s)	1267
DseDI	GACNNNNNNGTC	1 cut(s)	1267
Eco130I	CCWWGG	2 cut(s)	1197, 1297
Eco24I	GRGCYC	1 cut(s)	200
Eco32I	GATATC	1 cut(s)	493
Eco47I	GGWCC	3 cut(s)	69, 389, 1061
Eco57I	CTGAAG	1 cut(s)	335
Eco81I	CCTNAGG	1 cut(s)	663
Eco88I	CYCGRG	1 cut(s)	690
EcoNI	CCTNNNNNAGG	1 cut(s)	450
EcoO109I	RGGNCCY	1 cut(s)	69
EcoRI	GAATTC	1 cut(s)	735
EcoRII	CCWGG	2 cut(s)	238, 646
EcoRV	GATATC	1 cut(s)	493
EcoT14I	CCWWGG	2 cut(s)	1197, 1297
EcoT38I	GRGCYC	1 cut(s)	200
ErhI	CCWWGG	2 cut(s)	1197, 1297
Esp3I	CGTCTC	1 cut(s)	687
FaeI	CATG	3 cut(s)	323, 365, 1354
FaqI	GGGAC	2 cut(s)	55, 1334
FatI	CATG	3 cut(s)	319, 361, 1350
FbaI	TGATCA	1 cut(s)	1158
Fnu4HI	GCNGC	6 cut(s)	130, 288, 342, 684, 1131, 1221
FriOI	GRGCYC	1 cut(s)	200
Fsp4HI	GCNGC	6 cut(s)	130, 288, 342, 684, 1131, 1221
FspBI	CTAG	6 cut(s)	449, 1047, 1184, 1224, 1247, 1298
GluI	GCNGC	6 cut(s)	130, 288, 342, 684, 1131, 1221
GsuI	CTGGAG	1 cut(s)	312
HaeIII	GGCC	1 cut(s)	529
HapII	CCGG	1 cut(s)	1318
Hin1II	CATG	3 cut(s)	323, 365, 1354
HincII	GTYRAC	2 cut(s)	57, 300
HindII	GTYRAC	2 cut(s)	57, 300
HindIII	AAGCTT	1 cut(s)	920
HinfI	GANTC	2 cut(s)	855, 1105
HpaI	GTTAAC	2 cut(s)	57, 300
HpaII	CCGG	1 cut(s)	1318
HphI	GGTGA	3 cut(s)	722, 850, 900
Hpy166II	GTNNAC	3 cut(s)	57, 233, 300
Hpy188I	TCNGA	4 cut(s)	148, 437, 952, 1346
Hpy188III	TCNNGA	9 cut(s)	320, 470, 520, 690, 847, 1109, 1184, 1247, 1334
Hpy8I	GTNNAC	3 cut(s)	57, 233, 300
HpyAV	CCTTC	2 cut(s)	204, 1175
HpyCH4III	ACNGT	3 cut(s)	1024, 1119, 1285
HpyCH4IV	ACGT	1 cut(s)	576
HpyCH4V	TGCA	8 cut(s)	165, 233, 377, 707, 901, 1088, 1097, 1154
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1076, 1085, 1094
HpyF3I	CTNAG	1 cut(s)	663
HpySE526I	ACGT	1 cut(s)	576
Hsp92II	CATG	3 cut(s)	323, 365, 1354
KpnI	GGTACC	1 cut(s)	1197
Ksp22I	TGATCA	1 cut(s)	1158
KspAI	GTTAAC	2 cut(s)	57, 300
Kzo9I	GATC	3 cut(s)	169, 478, 1158
LmnI	GCTCC	1 cut(s)	195
Lsp1109I	GCAGC	6 cut(s)	116, 299, 328, 670, 1117, 1207
MaeI	CTAG	6 cut(s)	449, 1047, 1184, 1224, 1247, 1298
MaeII	ACGT	1 cut(s)	576
MaeIII	GTNAC	2 cut(s)	766, 856
MalI	GATC	3 cut(s)	171, 480, 1160
MboI	GATC	3 cut(s)	169, 478, 1158
MboII	GAAGA	5 cut(s)	12, 27, 132, 341, 1160
MfeI	CAATTG	1 cut(s)	740
MflI	RGATCY	1 cut(s)	169
MhlI	GDGCHC	2 cut(s)	200, 235
MlyI	GAGTC	1 cut(s)	864
MslI	CAYNNNNRTG	1 cut(s)	324
MspA1I	CMGCKG	2 cut(s)	290, 560
MspCI	CTTAAG	1 cut(s)	818
MspI	CCGG	1 cut(s)	1318
MspR9I	CCNGG	3 cut(s)	240, 648, 1319
MunI	CAATTG	1 cut(s)	740
MvaI	CCWGG	2 cut(s)	240, 648
MwoI	GCNNNNNNNGC	3 cut(s)	1076, 1085, 1094
NciI	CCSGG	1 cut(s)	1319
NdeII	GATC	3 cut(s)	169, 478, 1158
NlaIII	CATG	3 cut(s)	323, 365, 1354
NlaIV	GGNNCC	5 cut(s)	71, 171, 197, 1062, 1195
NmuCI	GTSAC	1 cut(s)	856
NspI	RCATGY	1 cut(s)	1354
NspV	TTCGAA	1 cut(s)	926
PagI	TCATGA	1 cut(s)	319
PaqCI	CACCTGC	1 cut(s)	698
PciI	ACATGT	1 cut(s)	1350
PcsI	WCGNNNNNNNCGW	1 cut(s)	1268
PfeI	GAWTC	1 cut(s)	1105
PfoI	TCCNGGA	1 cut(s)	1317
PkrI	GCNGC	6 cut(s)	131, 289, 343, 685, 1132, 1222
PleI	GAGTC	1 cut(s)	863
PpsI	GAGTC	1 cut(s)	863
PpuMI	RGGWCCY	1 cut(s)	69
PscI	ACATGT	1 cut(s)	1350
PshBI	ATTAAT	1 cut(s)	9
Psp5II	RGGWCCY	1 cut(s)	69
Psp6I	CCWGG	2 cut(s)	238, 646
PspGI	CCWGG	2 cut(s)	238, 646
PspN4I	GGNNCC	5 cut(s)	71, 171, 197, 1062, 1195
PspPI	GGNCC	3 cut(s)	69, 389, 1061
PspPPI	RGGWCCY	1 cut(s)	69
PsuI	RGATCY	1 cut(s)	169
PvuII	CAGCTG	2 cut(s)	290, 560
RsaI	GTAC	2 cut(s)	633, 1195
RsaNI	GTAC	2 cut(s)	632, 1194
RseI	CAYNNNNRTG	1 cut(s)	324
SatI	GCNGC	6 cut(s)	130, 288, 342, 684, 1131, 1221
Sau3AI	GATC	3 cut(s)	169, 478, 1158
Sau96I	GGNCC	3 cut(s)	69, 389, 1061
SchI	GAGTC	1 cut(s)	864
ScrFI	CCNGG	3 cut(s)	240, 648, 1319
SduI	GDGCHC	2 cut(s)	200, 235
SfuI	TTCGAA	1 cut(s)	926
SinI	GGWCC	3 cut(s)	69, 389, 1061
SmiMI	CAYNNNNRTG	1 cut(s)	324
SmlI	CTYRAG	4 cut(s)	518, 606, 722, 818
SmoI	CTYRAG	4 cut(s)	518, 606, 722, 818
SspI	AATATT	1 cut(s)	534
SspMI	CTAG	6 cut(s)	449, 1047, 1184, 1224, 1247, 1298
StyD4I	CCNGG	3 cut(s)	238, 646, 1317
StyI	CCWWGG	2 cut(s)	1197, 1297
TaaI	ACNGT	3 cut(s)	1024, 1119, 1285
TaiI	ACGT	1 cut(s)	579
TaqI	TCGA	4 cut(s)	670, 846, 926, 1103
TatI	WGTACW	1 cut(s)	631
TfiI	GAWTC	1 cut(s)	1105
TscAI	CASTG	1 cut(s)	1161
TseFI	GTSAC	1 cut(s)	856
TseI	GCWGC	6 cut(s)	129, 287, 341, 683, 1130, 1220
Tsp45I	GTSAC	1 cut(s)	856
TspDTI	ATGAA	6 cut(s)	37, 132, 149, 308, 336, 1195
TspRI	CASTG	1 cut(s)	1161
Vha464I	CTTAAG	1 cut(s)	818
VneI	GTGCAC	1 cut(s)	231
VpaK11BI	GGWCC	3 cut(s)	69, 389, 1061
VspI	ATTAAT	1 cut(s)	9
XagI	CCTNNNNNAGG	1 cut(s)	450
XapI	RAATTY	4 cut(s)	371, 404, 511, 735
XbaI	TCTAGA	2 cut(s)	1183, 1246
XceI	RCATGY	1 cut(s)	1354
XmaJI	CCTAGG	1 cut(s)	1297
XspI	CTAG	6 cut(s)	449, 1047, 1184, 1224, 1247, 1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.