Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0011954

Physical Location & Seq

Genomic Coordinates Reverse (-)

59027002 .. 59032505

Gene Structure 5504 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG406900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGAACTTCAGGTTCCAGAACCTTCTCGGAGCGCCGTACCGGGGAGGGAACGCCGTCATCGTCAACAACACGGAGCTAATCTCACCGGTGGGGAACAGAGTCTCCGTCACCAACCTCCGTGACTCCGAGACCATAACCCTACCCATCCAATCCTCCTCCAACATAACCCGCCTCGCCGCCTCCCCCGACGGCGTCTTCCTCCTCACCGTCGACGAGAAGCACCGCTGCCTCTTCATCAACCTCCGCCGCCGCGTGGTTCTCCACCGCATTTCCTTCAAGAGCGCCGTCGCCGCCGTCAAGTTCAGCCCCGACGGCGGTTACATTGCGGTGGCCACCGGTAAGTTGGTCCAGATTTGGCGCTCGCCTGGGTTTAAGAAAGAGTTCTTTCCGTTCGAATTGGTTAAGACTTTTGCTGACTGTGATGATAAGGTCACGGCGTTGGAGTGGAGCCCCGACTCGGTTTATGTGCTGGCCGGGTCGAAAGACTTGACTGCTAGGATTTTCTGTATGAAAAAGTTTAAATTGGGAGGGGTAAAGATTAAGCCTTTTATGTTTTTGGGGCATAGGGATACAGTAGTGGGTGTGTTTTTCGGTGTTGATAAGAAAGCCGGTAAGGTAAGCCGGGTTTATACAGTTGCGCGTGACGGTTATATGTTTGTGTGGGGTTTGAGTGGAGATGATGAGGAGGTGCCGTCTCCCGGGACACCGGAGAGGGAGAGGGATGGGGAAGGCAATGTGGAAGGTGATGGTAGTGTTGTCATGAAGAAAAGGAAGGGTTTCGAAGGAAATGGCGGTGAGTTGGATAAAGAAGGTGGGTATTTGATAAATAGGAAATGGGAGTTGTTGAAGAAAGAAAGTTTTGGGCAGGCGTCAATGAAGGTGACGGCATGTGATTATCATAGGGGGCTGGATATGCTGGTTGTGGGGTTCTCAAAGGGTGTATTTGCGCTGTATCAAATGCCGGATTTCGTGTGCATTCACAGATTGTCGATATCTAGAGAGAAAATTACAACAGCAGTTTTTAATGAGCTTGGGAATTGGCTGACATTTGGGTGTGCGAAACTTGGACAGTTGCTTGTATGGGAGTGGCGGTCTGAGAGTTATATTTTGAAGCAGCAGGGGCATTACTTTGATGTGAATTGTGTTGCTTATTCGCCTGATTCACAACTCTTGGCCACTGGGGCAGATGATAACAAAGTGAAGGTCTGGAATGTTTCTTCAGGATTCTGTTTTGTAACATTCTCTGAGCATGAAAATGCAATAACTGCACTCCACTTTTTGGCTAACAAAAATACGCTCTTAAGTGCCTCTTTGGATGGGACTGTTCGTGCATGGGATTTTTTCCGTTATCGAAATTTTAAGACATATACCACCGCAGAACCAAGACAATTTGTTTCTTTGACAGCAACTCAGAGTGGTGAAGTGATTTGTGCTGGAACTCTTGATACCTTTGAGATTTTTGTTTGGGATATGAGGACAGGCAACGTGAAGGACATTCTTAGTGGTCATGAAGGTCCTGTACATGGTTTGGCGTTTTCTCCTACAGATGCAAATGTTTTAGCTTCATCGTCGTGGGACAGAACAGTTCGGTTGTGGGATGTTTTTGAATCAAAAGGTGGTGTTGAAACATTTCCACACACACATGATGTTCTTACAGTAGTTTACCGCCCAGATGGAAAGCAATTGGCTTGCAGCACATTAGAAGGTCATATTCATTTCTGGGACCCAATTGATGGCGTATTAATGTATACCATTGAGGGTCGTAGGGATATTTCTGGTGGTCGTCTTATGACTGATCGTAGAACAGCTGCCAACTCAAGTTCAGGAAAGTTCTTTACAACCTTGTGTTATTCTGCTGATGGGAGTTACATATTAGCTGGGGGAAGTAGTAAATACATCTGCATGTATGATGTTGCTGATCAGGTATTACTGCGGAAGTTTCAAATAACTCACAATTTATCTTTGGATGGAGTTCTTGACTTCTTAAACTCAAAGAATATGACAGATGCTGGCCCCCTGGATCTAATTGATAATGATGACAGTGATATAGAAGAAGGTATTGACAAACAAACCCGGGGAAAGTTGGGTTATGATTTACCAGGGTCCATGCCTAATCGTGGAAGGCCTGTTGTTAGAACTAAATGCCTGAAAATTTCACCCACTGGTAGGAGCTTCTCAGCAGCAACAACAGAGGGAGTTCTAGTTTATTCTATTGATGATTCCTTTATCTTTGACCCAACTGATCTCGACATGGATGTCACGCCTGAGGTACTTGGTGAAACATTAATATTACTACTTAACAATGCCATTGCTTATGATAGAGTTGCAGTTGTGAAAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000028 GO:0000151 GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009553 GO:0009743 GO:0009744 GO:0009987 GO:0010033 GO:0010119 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0022607 GO:0022613 GO:0022618 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034285 GO:0034388 GO:0034470 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042255 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065003 GO:0065007 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901700 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp12 WD40

Protein Analysis

780

Amino Acids

86.56

Weight (kDa)

6.16

Isoelectric Point (pI)

34.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	38 - 172	1.3e-11	THOC3 beta-propeller domain
WD40_Prp19	PF24814	39 - 162	2.4e-11	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	58 - 176	1.2e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	93 - 200	1.1e-09	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	282 - 489	6.5e-14	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	287 - 404	9.7e-07	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	287 - 481	3.1e-21	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	288 - 458	1.5e-18	CDC20/Fizzy WD40 domain
WDR55	PF24796	293 - 575	2.4e-22	WDR55
Beta-prop_THOC3	PF25174	294 - 426	6.7e-15	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	294 - 482	6.1e-25	WDR5 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	311 - 428	5.7e-06	MABP1/WDR62 first WD40 domain
Beta-prop_EML	PF23409	313 - 481	7e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	317 - 482	1.2e-24	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	338 - 475	3e-12	Echinoderm microtubule-associated protein second beta-propeller
WDR90_beta-prop_4th	PF23342	350 - 442	4.8e-06	WDR90, 4th beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	354 - 574	3e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	354 - 466	3.7e-09	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	362 - 533	4.9e-21	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	368 - 489	1.7e-15	WDR3 second beta-propeller domain
WD40	PF00400	371 - 404	1.2e-09	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	372 - 492	4.9e-10	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	373 - 451	1.1e-06	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	378 - 458	1.4e-14	WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	381 - 447	8.6e-06	WDR90/POC16, second beta-propeller
EIF3I	PF24805	381 - 484	3.7e-07	EIF3I
Beta-prop_WDR75_1st	PF23869	385 - 463	1.5e-06	WD repeat-containing protein 75 first beta-propeller
WD40	PF00400	410 - 446	2.1e-06	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	419 - 592	4.1e-15	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	423 - 579	1.2e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	447 - 567	5.4e-10	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	466 - 654	8.4e-18	WDHD1 first WD40 domain
EIF3I	PF24805	472 - 649	1.4e-06	EIF3I
Beta-prop_WDR5	PF25175	473 - 648	9.4e-18	WDR5 beta-propeller domain
WD40_Prp19	PF24814	474 - 534	2.3e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	485 - 607	6.1e-15	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	485 - 652	7e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	488 - 576	1.1e-16	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	497 - 588	5.1e-06	TEP-1 second beta-propeller
WD40	PF00400	498 - 533	1.2e-08	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	507 - 643	1.6e-08	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

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Orthologous Genes (Group: OG0011954)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15440 AT1G15440
fragaria_vesca	FvH4_6g30400 FvH4_6g30400
malus_domestica	MD09G1221400.v1.1 MD17G1203900.v1.1
prunus_persica	Prupe.3G078900_v2.0.a1
pyrus_communis	pycom1094g00080 pycom17g20750
rosa_chinensis	RchiOBHm_Chr2g0136751
rosa_laevigata	RLG00000019569
rosa_multiflora	Rmu_sc0001299.1_g000021
rosa_roxburghii	Rroxscaffold_2G00108100
rosa_rugosa	Rorug02G0332800
rosa_samantha	Rh2AG383600 Rh2BG390300 Rh2CG370100 Rh2DG406900
rosa_wichuraiana	Rw2G031370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2255
AccB1I	GGYRCC	1 cut(s)	688
AccB7I	CCANNNNNTGG	2 cut(s)	1279, 1733
AccI	GTMKAC	2 cut(s)	210, 1748
AccII	CGCG	2 cut(s)	252, 640
AclWI	GGATC	1 cut(s)	2028
AcoI	YGGCCR	3 cut(s)	330, 471, 1173
AcsI	RAATTY	2 cut(s)	1354, 2151
AcuI	CTGAAG	1 cut(s)	1203
AcyI	GRCGYC	2 cut(s)	192, 869
AfaI	GTAC	3 cut(s)	38, 1521, 2271
AflII	CTTAAG	1 cut(s)	1300
AgeI	ACCGGT	2 cut(s)	85, 335
AgsI	TTSAA	6 cut(s)	277, 847, 1111, 1607, 1625, 1943
AjnI	CCWGG	3 cut(s)	364, 2016, 2098
AloI	GAACNNNNNNTCC	2 cut(s)	86, 118
AluBI	AGCT	6 cut(s)	76, 1030, 1562, 1808, 1877, 2172
AluI	AGCT	6 cut(s)	76, 1030, 1562, 1808, 1877, 2172
Alw26I	GTCTC	3 cut(s)	106, 122, 699
AlwI	GGATC	1 cut(s)	2028
AlwNI	CAGNNNCTG	1 cut(s)	2009
Ama87I	CYCGRG	2 cut(s)	698, 2073
AoxI	GGCC	5 cut(s)	330, 471, 1173, 2011, 2123
ApeKI	GCWGC	5 cut(s)	225, 1114, 1692, 1808, 2180
ApoI	RAATTY	2 cut(s)	1354, 2151
ArsI	GACNNNNNNTTYG	2 cut(s)	1188, 1220
AseI	ATTAAT	2 cut(s)	1742, 2285
AsiGI	ACCGGT	2 cut(s)	85, 335
Asp700I	GAANNNNTTC	3 cut(s)	380, 776, 1629
AspLEI	GCGC	5 cut(s)	34, 284, 360, 640, 949
AspS9I	GGNCC	5 cut(s)	346, 1514, 1723, 2012, 2103
AsuC2I	CCSGG	7 cut(s)	41, 475, 623, 699, 700, 2074, 2075
AsuHPI	GGTGA	9 cut(s)	75, 100, 196, 755, 806, 892, 1430, 2148, 2288
AsuII	TTCGAA	2 cut(s)	393, 780
AvaI	CYCGRG	2 cut(s)	698, 2073
AvaII	GGWCC	4 cut(s)	346, 1514, 1723, 2103
AxyI	CCTNAGG	1 cut(s)	2265
BaeI	ACNNNNGTAYC	2 cut(s)	561, 594
BalI	TGGCCA	2 cut(s)	332, 1175
BanI	GGYRCC	1 cut(s)	688
BanII	GRGCYC	1 cut(s)	452
BarI	GAAGNNNNNNTAC	2 cut(s)	1503, 1535
BbsI	GAAGAC	1 cut(s)	187
BbvI	GCAGC	5 cut(s)	212, 1126, 1704, 1795, 2192
BccI	CCATC	8 cut(s)	152, 716, 740, 1310, 1667, 1727, 1853, 1961
BceAI	ACGGC	9 cut(s)	19, 38, 205, 269, 278, 328, 450, 676, 900
BciT130I	CCWGG	3 cut(s)	366, 2018, 2100
BciVI	GTATCC	1 cut(s)	562
BclI	TGATCA	1 cut(s)	1918
BcnI	CCSGG	7 cut(s)	41, 475, 623, 699, 700, 2074, 2075
BcoDI	GTCTC	3 cut(s)	106, 122, 699
BfaI	CTAG	3 cut(s)	495, 996, 2201
BfmI	CTRYAG	1 cut(s)	1542
BfoI	RGCGCY	3 cut(s)	35, 285, 361
BfrI	CTTAAG	1 cut(s)	1300
BfuI	GTATCC	1 cut(s)	562
BglI	GCCNNNNNGGC	2 cut(s)	312, 1181
BisI	GCNGC	9 cut(s)	177, 226, 247, 250, 291, 1115, 1693, 1809, 2181
BlsI	GCNGC	9 cut(s)	178, 227, 248, 251, 292, 1116, 1694, 1810, 2182
Bme18I	GGWCC	4 cut(s)	346, 1514, 1723, 2103
BmeT110I	CYCGRG	2 cut(s)	698, 2073
BmgT120I	GGNCC	5 cut(s)	346, 1514, 1723, 2012, 2103
BmiI	GGNNCC	7 cut(s)	14, 449, 690, 1724, 1725, 2014, 2104
BmrI	ACTGGG	1 cut(s)	1188
BmsI	GCATC	2 cut(s)	1537, 1996
BmuI	ACTGGG	1 cut(s)	1188
BpiI	GAAGAC	1 cut(s)	187
BplI	GAGNNNNNCTC	2 cut(s)	65, 97
Bpu14I	TTCGAA	2 cut(s)	393, 780
BpuEI	CTTGAG	1 cut(s)	1801
BpuMI	CCSGG	7 cut(s)	41, 475, 623, 699, 700, 2074, 2075
BsaHI	GRCGYC	2 cut(s)	192, 869
BsaI	GGTCTC	1 cut(s)	122
BsaJI	CCNNGG	7 cut(s)	40, 365, 698, 2016, 2073, 2074, 2099
BsaWI	WCCGGW	3 cut(s)	85, 335, 706
BsaXI	ACNNNNNCTCC	8 cut(s)	86, 116, 677, 707, 1076, 1106, 2187, 2217
Bse118I	RCCGGY	3 cut(s)	85, 335, 608
Bse1I	ACTGG	2 cut(s)	1183, 2167
Bse21I	CCTNAGG	1 cut(s)	2265
Bse3DI	GCAATG	3 cut(s)	321, 739, 2307
BseBI	CCWGG	3 cut(s)	366, 2018, 2100
BseDI	CCNNGG	7 cut(s)	40, 365, 698, 2016, 2073, 2074, 2099
BseGI	GGATG	6 cut(s)	144, 727, 1321, 1603, 1972, 2260
BseMI	GCAATG	3 cut(s)	321, 739, 2307
BseMII	CTCAG	5 cut(s)	1086, 1236, 1424, 2190, 2256
BseNI	ACTGG	2 cut(s)	1183, 2167
BseRI	GAGGAG	3 cut(s)	145, 191, 698
BseXI	GCAGC	5 cut(s)	212, 1126, 1704, 1795, 2192
BseYI	CCCAGC	1 cut(s)	1877
BsgI	GTGCAG	1 cut(s)	1251
Bsh1236I	CGCG	2 cut(s)	252, 640
BshFI	GGCC	5 cut(s)	332, 473, 1175, 2013, 2125
BshNI	GGYRCC	1 cut(s)	688
BshTI	ACCGGT	2 cut(s)	85, 335
BsiHKCI	CYCGRG	2 cut(s)	698, 2073
BslFI	GGGAC	4 cut(s)	715, 1333, 1589, 1736
BsmAI	GTCTC	3 cut(s)	106, 122, 699
BsmBI	CGTCTC	1 cut(s)	699
BsmFI	GGGAC	4 cut(s)	715, 1333, 1589, 1736
BsmI	GAATGC	1 cut(s)	975
BsnI	GGCC	5 cut(s)	332, 473, 1175, 2013, 2125
Bso31I	GGTCTC	1 cut(s)	122
BsoBI	CYCGRG	2 cut(s)	698, 2073
Bsp119I	TTCGAA	2 cut(s)	393, 780
Bsp1286I	GDGCHC	1 cut(s)	452
Bsp1407I	TGTACA	1 cut(s)	1519
Bsp143I	GATC	4 cut(s)	1795, 1918, 2020, 2242
BspANI	GGCC	5 cut(s)	332, 473, 1175, 2013, 2125
BspCNI	CTCAG	5 cut(s)	1087, 1237, 1423, 2189, 2257
BspFNI	CGCG	2 cut(s)	252, 640
BspHI	TCATGA	2 cut(s)	759, 1507
BspLI	GGNNCC	7 cut(s)	14, 449, 690, 1724, 1725, 2014, 2104
BspPI	GGATC	1 cut(s)	2028
BspT104I	TTCGAA	2 cut(s)	393, 780
BspT107I	GGYRCC	1 cut(s)	688
BspTI	CTTAAG	1 cut(s)	1300
BspTNI	GGTCTC	1 cut(s)	122
BsrDI	GCAATG	3 cut(s)	321, 739, 2307
BsrFI	RCCGGY	3 cut(s)	85, 335, 608
BsrGI	TGTACA	1 cut(s)	1519
BsrI	ACTGG	2 cut(s)	1183, 2167
BssAI	RCCGGY	3 cut(s)	85, 335, 608
BssECI	CCNNGG	7 cut(s)	40, 365, 698, 2016, 2073, 2074, 2099
BssMI	GATC	4 cut(s)	1795, 1918, 2020, 2242
BssNAI	GTATAC	1 cut(s)	1749
BssNI	GRCGYC	2 cut(s)	192, 869
Bst1107I	GTATAC	1 cut(s)	1749
Bst2UI	CCWGG	3 cut(s)	366, 2018, 2100
Bst6I	CTCTTC	1 cut(s)	236
BstACI	GRCGYC	2 cut(s)	192, 869
BstAFI	CTTAAG	1 cut(s)	1300
BstAPI	GCANNNNNTGC	1 cut(s)	1265
BstAUI	TGTACA	1 cut(s)	1519
BstBI	TTCGAA	2 cut(s)	393, 780
BstC8I	GCNNGC	5 cut(s)	362, 471, 867, 1690, 2011
BstDEI	CTNAG	6 cut(s)	1095, 1245, 1410, 1499, 2176, 2265
BstF5I	GGATG	6 cut(s)	144, 727, 1321, 1603, 1972, 2260
BstFNI	CGCG	2 cut(s)	252, 640
BstH2I	RGCGCY	3 cut(s)	35, 285, 361
BstHHI	GCGC	5 cut(s)	34, 284, 360, 640, 949
BstKTI	GATC	4 cut(s)	1798, 1921, 2023, 2245
BstMAI	GTCTC	3 cut(s)	106, 122, 699
BstMBI	GATC	4 cut(s)	1795, 1918, 2020, 2242
BstMWI	GCNNNNNNNGC	6 cut(s)	290, 312, 913, 1120, 1181, 1265
BstNI	CCWGG	3 cut(s)	366, 2018, 2100
BstNSI	RCATGY	2 cut(s)	891, 1906
BstSFI	CTRYAG	1 cut(s)	1542
BstUI	CGCG	2 cut(s)	252, 640
BstV1I	GCAGC	5 cut(s)	212, 1126, 1704, 1795, 2192
BstV2I	GAAGAC	1 cut(s)	187
BstX2I	RGATCY	1 cut(s)	2020
BstYI	RGATCY	1 cut(s)	2020
BstZ17I	GTATAC	1 cut(s)	1749
Bsu36I	CCTNAGG	1 cut(s)	2265
BsuI	GTATCC	1 cut(s)	562
BsuRI	GGCC	5 cut(s)	332, 473, 1175, 2013, 2125
BtsCI	GGATG	6 cut(s)	144, 727, 1321, 1603, 1972, 2260
BtsIMutI	CAGTG	3 cut(s)	1176, 2047, 2160
Cac8I	GCNNGC	5 cut(s)	362, 471, 867, 1690, 2011
CaiI	CAGNNNCTG	1 cut(s)	2009
CciI	TCATGA	2 cut(s)	759, 1507
CfoI	GCGC	5 cut(s)	34, 284, 360, 640, 949
Cfr10I	RCCGGY	3 cut(s)	85, 335, 608
Cfr13I	GGNCC	5 cut(s)	346, 1514, 1723, 2012, 2103
Cfr9I	CCCGGG	2 cut(s)	698, 2073
CseI	GACGC	2 cut(s)	181, 858
Csp6I	GTAC	3 cut(s)	37, 1520, 2270
CspAI	ACCGGT	2 cut(s)	85, 335
CviQI	GTAC	3 cut(s)	37, 1520, 2270
DdeI	CTNAG	6 cut(s)	1095, 1245, 1410, 1499, 2176, 2265
DpnI	GATC	4 cut(s)	1797, 1920, 2022, 2244
DpnII	GATC	4 cut(s)	1795, 1918, 2020, 2242
DraI	TTTAAA	1 cut(s)	520
DrdI	GACNNNNNNGTC	1 cut(s)	2255
DseDI	GACNNNNNNGTC	1 cut(s)	2255
EaeI	YGGCCR	3 cut(s)	330, 471, 1173
Eam1104I	CTCTTC	1 cut(s)	236
EarI	CTCTTC	1 cut(s)	236
EciI	GGCGGA	1 cut(s)	233
Eco147I	AGGCCT	1 cut(s)	2125
Eco24I	GRGCYC	1 cut(s)	452
Eco31I	GGTCTC	1 cut(s)	122
Eco32I	GATATC	1 cut(s)	993
Eco47I	GGWCC	4 cut(s)	346, 1514, 1723, 2103
Eco57I	CTGAAG	1 cut(s)	1203
Eco81I	CCTNAGG	1 cut(s)	2265
Eco88I	CYCGRG	2 cut(s)	698, 2073
EcoO109I	RGGNCCY	2 cut(s)	1514, 1723
EcoRII	CCWGG	3 cut(s)	364, 2016, 2098
EcoRV	GATATC	1 cut(s)	993
EcoT38I	GRGCYC	1 cut(s)	452
Esp3I	CGTCTC	1 cut(s)	699
FalI	AAGNNNNNCTT	2 cut(s)	1294, 1326
FaqI	GGGAC	4 cut(s)	715, 1333, 1589, 1736
FauI	CCCGC	1 cut(s)	176
FbaI	TGATCA	1 cut(s)	1918
FblI	GTMKAC	2 cut(s)	210, 1748
Fnu4HI	GCNGC	9 cut(s)	177, 226, 247, 250, 291, 1115, 1693, 1809, 2181
FokI	GGATG	6 cut(s)	131, 734, 1328, 1610, 1979, 2267
FriOI	GRGCYC	1 cut(s)	452
Fsp4HI	GCNGC	9 cut(s)	177, 226, 247, 250, 291, 1115, 1693, 1809, 2181
FspBI	CTAG	3 cut(s)	495, 996, 2201
GlaI	GCGC	5 cut(s)	33, 283, 359, 639, 948
GluI	GCNGC	9 cut(s)	177, 226, 247, 250, 291, 1115, 1693, 1809, 2181
GsaI	CCCAGC	1 cut(s)	1881
HaeII	RGCGCY	3 cut(s)	35, 285, 361
HaeIII	GGCC	5 cut(s)	332, 473, 1175, 2013, 2125
HgaI	GACGC	2 cut(s)	181, 858
HhaI	GCGC	5 cut(s)	34, 284, 360, 640, 949
Hin1I	GRCGYC	2 cut(s)	192, 869
Hin6I	GCGC	5 cut(s)	32, 282, 358, 638, 947
HinP1I	GCGC	5 cut(s)	32, 282, 358, 638, 947
HincII	GTYRAC	2 cut(s)	64, 211
HindII	GTYRAC	2 cut(s)	64, 211
HinfI	GANTC	7 cut(s)	99, 122, 455, 1160, 1224, 1607, 2219
HphI	GGTGA	9 cut(s)	75, 100, 196, 755, 806, 892, 1430, 2148, 2288
Hpy166II	GTNNAC	4 cut(s)	64, 211, 1663, 1749
Hpy188I	TCNGA	5 cut(s)	29, 127, 1096, 1246, 1413
Hpy8I	GTNNAC	4 cut(s)	64, 211, 1663, 1749
Hpy99I	CGWCG	6 cut(s)	191, 212, 215, 290, 314, 1573
HpyCH4IV	ACGT	1 cut(s)	1485
HpyCH4V	TGCA	8 cut(s)	975, 1259, 1268, 1331, 1550, 1692, 1902, 2327
HpyF10VI	GCNNNNNNNGC	6 cut(s)	290, 312, 913, 1120, 1181, 1265
HpyF3I	CTNAG	6 cut(s)	1095, 1245, 1410, 1499, 2176, 2265
HpySE526I	ACGT	1 cut(s)	1485
Hsp92I	GRCGYC	2 cut(s)	192, 869
HspAI	GCGC	5 cut(s)	32, 282, 358, 638, 947
KflI	GGGWCCC	1 cut(s)	1723
Ksp22I	TGATCA	1 cut(s)	1918
Kzo9I	GATC	4 cut(s)	1795, 1918, 2020, 2242
LmnI	GCTCC	4 cut(s)	29, 73, 447, 2169
Lsp1109I	GCAGC	5 cut(s)	212, 1126, 1704, 1795, 2192
LweI	GCATC	2 cut(s)	1537, 1996
MaeI	CTAG	3 cut(s)	495, 996, 2201
MaeII	ACGT	1 cut(s)	1485
MaeIII	GTNAC	9 cut(s)	106, 119, 317, 430, 641, 880, 1234, 1865, 2257
MalI	GATC	4 cut(s)	1797, 1920, 2022, 2244
MboI	GATC	4 cut(s)	1795, 1918, 2020, 2242
MboII	GAAGA	6 cut(s)	187, 223, 775, 859, 1209, 2063
MfeI	CAATTG	2 cut(s)	1682, 1728
MflI	RGATCY	1 cut(s)	2020
MhlI	GDGCHC	1 cut(s)	452
MlsI	TGGCCA	2 cut(s)	332, 1175
MluNI	TGGCCA	2 cut(s)	332, 1175
MlyI	GAGTC	3 cut(s)	108, 116, 449
MmeI	TCCRAC	3 cut(s)	183, 420, 780
Mox20I	TGGCCA	2 cut(s)	332, 1175
MroXI	GAANNNNTTC	3 cut(s)	380, 776, 1629
MscI	TGGCCA	2 cut(s)	332, 1175
MslI	CAYNNNNRTG	6 cut(s)	326, 1051, 1254, 1570, 1641, 1901
Msp20I	TGGCCA	2 cut(s)	332, 1175
MspA1I	CMGCKG	2 cut(s)	225, 1808
MspCI	CTTAAG	1 cut(s)	1300
MunI	CAATTG	2 cut(s)	1682, 1728
Mva1269I	GAATGC	1 cut(s)	975
MvaI	CCWGG	3 cut(s)	366, 2018, 2100
MvnI	CGCG	2 cut(s)	252, 640
MwoI	GCNNNNNNNGC	6 cut(s)	290, 312, 913, 1120, 1181, 1265
NciI	CCSGG	7 cut(s)	41, 475, 623, 699, 700, 2074, 2075
NdeII	GATC	4 cut(s)	1795, 1918, 2020, 2242
NlaIV	GGNNCC	7 cut(s)	14, 449, 690, 1724, 1725, 2014, 2104
NmuCI	GTSAC	6 cut(s)	106, 119, 430, 641, 880, 2257
NspI	RCATGY	2 cut(s)	891, 1906
NspV	TTCGAA	2 cut(s)	393, 780
PagI	TCATGA	2 cut(s)	759, 1507
PceI	AGGCCT	1 cut(s)	2125
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PctI	GAATGC	1 cut(s)	975
PdmI	GAANNNNTTC	3 cut(s)	380, 776, 1629
PfeI	GAWTC	4 cut(s)	1160, 1224, 1607, 2219
PflFI	GACNNNGTC	1 cut(s)	191
PflMI	CCANNNNNTGG	2 cut(s)	1279, 1733
PinAI	ACCGGT	2 cut(s)	85, 335
PkrI	GCNGC	9 cut(s)	178, 227, 248, 251, 292, 1116, 1694, 1810, 2182
PleI	GAGTC	3 cut(s)	107, 116, 449
PpsI	GAGTC	3 cut(s)	107, 116, 449
PpuMI	RGGWCCY	2 cut(s)	1514, 1723
PshBI	ATTAAT	2 cut(s)	1742, 2285
Psp5II	RGGWCCY	2 cut(s)	1514, 1723
Psp6I	CCWGG	3 cut(s)	364, 2016, 2098
PspFI	CCCAGC	1 cut(s)	1877
PspGI	CCWGG	3 cut(s)	364, 2016, 2098
PspN4I	GGNNCC	7 cut(s)	14, 449, 690, 1724, 1725, 2014, 2104
PspPI	GGNCC	5 cut(s)	346, 1514, 1723, 2012, 2103
PspPPI	RGGWCCY	2 cut(s)	1514, 1723
PsrI	GAACNNNNNNTAC	2 cut(s)	1429, 1461
PstNI	CAGNNNCTG	1 cut(s)	2009
PsuI	RGATCY	1 cut(s)	2020
PsyI	GACNNNGTC	1 cut(s)	191
PvuII	CAGCTG	1 cut(s)	1808
RsaI	GTAC	3 cut(s)	38, 1521, 2271
RsaNI	GTAC	3 cut(s)	37, 1520, 2270
RseI	CAYNNNNRTG	6 cut(s)	326, 1051, 1254, 1570, 1641, 1901
SalI	GTCGAC	1 cut(s)	209
SatI	GCNGC	9 cut(s)	177, 226, 247, 250, 291, 1115, 1693, 1809, 2181
Sau3AI	GATC	4 cut(s)	1795, 1918, 2020, 2242
Sau96I	GGNCC	5 cut(s)	346, 1514, 1723, 2012, 2103
SchI	GAGTC	3 cut(s)	108, 116, 449
SduI	GDGCHC	1 cut(s)	452
SfaNI	GCATC	2 cut(s)	1537, 1996
SfcI	CTRYAG	1 cut(s)	1542
SfuI	TTCGAA	2 cut(s)	393, 780
SgrAI	CRCCGGYG	1 cut(s)	85
SgrDI	CGTCGACG	1 cut(s)	209
SinI	GGWCC	4 cut(s)	346, 1514, 1723, 2103
SmaI	CCCGGG	2 cut(s)	700, 2075
SmiMI	CAYNNNNRTG	6 cut(s)	326, 1051, 1254, 1570, 1641, 1901
SmlI	CTYRAG	2 cut(s)	1300, 1816
SmoI	CTYRAG	2 cut(s)	1300, 1816
SseBI	AGGCCT	1 cut(s)	2125
SspI	AATATT	1 cut(s)	2289
SspMI	CTAG	3 cut(s)	495, 996, 2201
StuI	AGGCCT	1 cut(s)	2125
TaiI	ACGT	1 cut(s)	1488
TaqI	TCGA	7 cut(s)	210, 393, 479, 780, 989, 1351, 2247
TatI	WGTACW	1 cut(s)	1519
TauI	GCSGC	4 cut(s)	179, 249, 252, 293
TfiI	GAWTC	4 cut(s)	1160, 1224, 1607, 2219
TscAI	CASTG	3 cut(s)	1183, 2047, 2167
TseFI	GTSAC	6 cut(s)	106, 119, 430, 641, 880, 2257
TseI	GCWGC	5 cut(s)	225, 1114, 1692, 1808, 2180
Tsp45I	GTSAC	6 cut(s)	106, 119, 430, 641, 880, 2257
TspDTI	ATGAA	9 cut(s)	17, 223, 524, 776, 890, 1266, 1524, 1554, 1703
TspGWI	ACGGA	5 cut(s)	86, 94, 107, 378, 1334
TspMI	CCCGGG	2 cut(s)	698, 2073
TspRI	CASTG	3 cut(s)	1183, 2047, 2167
Tth111I	GACNNNGTC	1 cut(s)	191
Van91I	CCANNNNNTGG	2 cut(s)	1279, 1733
Vha464I	CTTAAG	1 cut(s)	1300
VpaK11BI	GGWCC	4 cut(s)	346, 1514, 1723, 2103
VspI	ATTAAT	2 cut(s)	1742, 2285
XapI	RAATTY	2 cut(s)	1354, 2151
XbaI	TCTAGA	1 cut(s)	995
XceI	RCATGY	2 cut(s)	891, 1906
XcmI	CCANNNNNNNNNTGG	1 cut(s)	340
XmaI	CCCGGG	2 cut(s)	698, 2073
XmiI	GTMKAC	2 cut(s)	210, 1748
XmnI	GAANNNNTTC	3 cut(s)	380, 776, 1629
XspI	CTAG	3 cut(s)	495, 996, 2201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.