Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0007870

Physical Location & Seq

Genomic Coordinates Reverse (-)

88075246 .. 88077683

Gene Structure 2438 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG656000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGGCAACGAAGGGTGCGATTGTCTCTGCTGCTAAGGCTCTTGCCTCACCACGCAGCTCTTCCGCTTCCACTTTTTTCTCCAGGCAGCTCCACGCAACTCCGGGGAGCAAGAAGATTGTTGGGGTGTTTTATAAGGCTAATGAGTATGCCAAACTCAACCCCAATTTTTTGGGTTGTGAGGAAAACGCTTTGGGTATAAGGGAGTGGCTTGAATCACAGGGACACAAGTATATTGTTACTGATGACAAGGAGGGACCAAATTGTGAGCTTGAGAAACACATTGAGGATATGCATGTTCTGATCACAACCCCTTTCCACCCTGCCTATGTCACGGCGGAGAGGATTAAAAAGGCCAAGAATTTGCAGCTTCTTTTGACTGCCGGAATTGGCTCTGATCACATTGACCTCAAGGCTGCCGCGGCGGCTGGACTGACCGTTGCTGAGGTCACCGGAAGCAATACTGTTTCGGTTGCGGAAGATGAGCTGATGAGGATCCTCATTCTTGTGAGGAACTTTGTTCCGGGGTATCAGCAGGTTGTTAATGGGGAGTGGAAGGTTGCAGCCATTTCCCACAGGGCTTATGATTTGGAAGGAAAGACAGTTGGTACTGTTGGAGCTGGAAGAATTGGCAAGCTTTTGATGCAAAGATTGAGGCCTTTCAACTGTAATATCCTCTATCATGATAGAATTAAGGCTGATGCTGAATTTGAAAAAGAGACGGGGGCTAAGTTTGAGGAGGATCTTGATAAGATGCTTCCAAAATGTGATGTTGTTGTTGTCAACACCCCACTAACAGAAAAGACAAGGGGGATGTTTGATAAAGAGAGAATTTCCAAGCTCAAGAAGGGAGTCCTGATCGTGAACAATGCCCGTGGGGCAATCATGGACACGCAAGCAGTTGTTGATGCTTGCAACAGTGGACACATAGCAGGATACAGTGGTGATGTTTGGAACCCCCAACCAGCTCCCAAAGACCATCCATGGCGTTACATGCCAAACCATGCCATGACCCCTCATACATCTGGCACCACAATTGATGGACAGTTGCGTTATGCTGCTGGTACCAAGGACATGCTCGACAGGTACTTCAAAGGCGAAGACTTTCCTGCTGAAAACTACATTGTCAAGGAGGGCAAGCTAGCAAGCCAGTACCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0009507 GO:0009536 GO:0009579 GO:0010035 GO:0010038 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko00630 ko00680 ko01100 ko01120 ko01200 map00630 map00680 map01100 map01120 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

385

Amino Acids

42.41

Weight (kDa)

8.12

Isoelectric Point (pI)

30.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	70 - 300	2.2e-15	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	163 - 341	1.2e-52	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007870)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14780 AT5G14780 AT5G14780
fragaria_vesca	FvH4_6g50630
malus_domestica	MD09G1034400.v1.1 MD17G1034800.v1.1
prunus_persica	Prupe.3G284000_v2.0.a1 Prupe.3G284000_v2.0.a1 Prupe.3G284000_v2.0.a1 Prupe.3G284000_v2.0.a1
pyrus_communis	pycom111g02730 pycom17g03210
rosa_chinensis	RchiOBHm_Chr2g0171431
rosa_laevigata	RLG00000022013
rosa_multiflora	Rmu_sc0007646.1_g000006 Rmu_sc0027265.1_g000001 Rmu_sc0030144.1_g000002
rosa_roxburghii	Rroxscaffold_2G00080660
rosa_rugosa	Rorug02G0554200
rosa_samantha	Rh2AG628500 Rh2CG607600 Rh2DG650000 Rh2DG656000
rosa_wichuraiana	Rw2G052060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	132
Acc36I	ACCTGC	1 cut(s)	523
Acc65I	GGTACC	1 cut(s)	1061
AccB1I	GGYRCC	2 cut(s)	1025, 1061
AccII	CGCG	1 cut(s)	419
AciI	CCGC	6 cut(s)	63, 335, 417, 419, 422, 473
AclWI	GGATC	3 cut(s)	487, 500, 747
AcsI	RAATTY	3 cut(s)	358, 704, 828
AfaI	GTAC	4 cut(s)	607, 1063, 1085, 1151
AgsI	TTSAA	4 cut(s)	212, 661, 710, 1090
AjnI	CCWGG	1 cut(s)	80
Alw26I	GTCTC	2 cut(s)	28, 710
AlwI	GGATC	3 cut(s)	487, 500, 747
AoxI	GGCC	2 cut(s)	351, 653
ApeKI	GCWGC	7 cut(s)	29, 54, 85, 364, 413, 560, 1055
ApoI	RAATTY	3 cut(s)	358, 704, 828
Asp700I	GAANNNNTTC	1 cut(s)	1101
Asp718I	GGTACC	1 cut(s)	1061
AspS9I	GGNCC	1 cut(s)	254
AsuC2I	CCSGG	2 cut(s)	102, 522
AsuHPI	GGTGA	3 cut(s)	39, 439, 953
AsuNHI	GCTAGC	1 cut(s)	1138
AvaII	GGWCC	1 cut(s)	254
BaeI	ACNNNNGTAYC	2 cut(s)	925, 958
BamHI	GGATCC	1 cut(s)	492
BanI	GGYRCC	2 cut(s)	1025, 1061
BbsI	GAAGAC	1 cut(s)	1104
BbvCI	CCTCAGC	1 cut(s)	441
BbvI	GCAGC	7 cut(s)	16, 66, 97, 376, 400, 572, 1042
BccI	CCATC	2 cut(s)	984, 1031
BceAI	ACGGC	1 cut(s)	348
BciT130I	CCWGG	1 cut(s)	82
BciVI	GTATCC	1 cut(s)	926
BclI	TGATCA	2 cut(s)	300, 394
BcnI	CCSGG	2 cut(s)	102, 522
BcoDI	GTCTC	2 cut(s)	28, 710
BfaI	CTAG	1 cut(s)	1139
BfuAI	ACCTGC	1 cut(s)	523
BfuI	GTATCC	1 cut(s)	926
BglI	GCCNNNNNGGC	2 cut(s)	422, 875
Bme1390I	CCNGG	3 cut(s)	82, 102, 522
Bme18I	GGWCC	1 cut(s)	254
BmgT120I	GGNCC	1 cut(s)	254
BmiI	GGNNCC	5 cut(s)	255, 494, 953, 1027, 1063
BmrFI	CCNGG	3 cut(s)	82, 102, 522
BmsI	GCATC	4 cut(s)	630, 688, 741, 895
BmtI	GCTAGC	1 cut(s)	1142
BpiI	GAAGAC	1 cut(s)	1104
BpmI	CTGGAG	1 cut(s)	64
Bpu10I	CCTNAGC	2 cut(s)	33, 441
BpuEI	CTTGAG	3 cut(s)	290, 392, 824
BpuMI	CCSGG	2 cut(s)	102, 522
BsaBI	GATNNNNATC	1 cut(s)	491
BsaJI	CCNNGG	6 cut(s)	101, 417, 521, 871, 980, 1065
BsaWI	WCCGGW	1 cut(s)	449
Bse1I	ACTGG	2 cut(s)	1147, 1153
Bse8I	GATNNNNATC	1 cut(s)	491
BseBI	CCWGG	1 cut(s)	82
BseDI	CCNNGG	6 cut(s)	101, 417, 521, 871, 980, 1065
BseGI	GGATG	2 cut(s)	816, 976
BseJI	GATNNNNATC	1 cut(s)	491
BseMII	CTCAG	1 cut(s)	432
BseNI	ACTGG	2 cut(s)	1147, 1153
BseRI	GAGGAG	1 cut(s)	749
BseXI	GCAGC	7 cut(s)	16, 66, 97, 376, 400, 572, 1042
Bsh1236I	CGCG	1 cut(s)	419
BshFI	GGCC	2 cut(s)	353, 655
BshNI	GGYRCC	2 cut(s)	1025, 1061
BsiSI	CCGG	4 cut(s)	101, 381, 450, 521
BslFI	GGGAC	2 cut(s)	234, 267
BsmAI	GTCTC	2 cut(s)	28, 710
BsmBI	CGTCTC	1 cut(s)	710
BsmFI	GGGAC	2 cut(s)	234, 267
BsnI	GGCC	2 cut(s)	353, 655
Bsp143I	GATC	5 cut(s)	300, 394, 492, 739, 855
Bsp19I	CCATGG	1 cut(s)	980
BspACI	CCGC	6 cut(s)	63, 335, 417, 419, 422, 473
BspANI	GGCC	2 cut(s)	353, 655
BspCNI	CTCAG	1 cut(s)	433
BspFNI	CGCG	1 cut(s)	419
BspHI	TCATGA	1 cut(s)	679
BspLI	GGNNCC	5 cut(s)	255, 494, 953, 1027, 1063
BspMI	ACCTGC	1 cut(s)	523
BspOI	GCTAGC	1 cut(s)	1142
BspPI	GGATC	3 cut(s)	487, 500, 747
BspQI	GCTCTTC	1 cut(s)	64
BspT107I	GGYRCC	2 cut(s)	1025, 1061
BsrI	ACTGG	2 cut(s)	1147, 1153
BssECI	CCNNGG	6 cut(s)	101, 417, 521, 871, 980, 1065
BssMI	GATC	5 cut(s)	300, 394, 492, 739, 855
BssT1I	CCWWGG	2 cut(s)	980, 1065
Bst2UI	CCWGG	1 cut(s)	82
Bst4CI	ACNGT	8 cut(s)	436, 463, 601, 610, 665, 917, 938, 1044
Bst6I	CTCTTC	1 cut(s)	64
BstC8I	GCNNGC	6 cut(s)	632, 894, 910, 1136, 1140, 1144
BstDEI	CTNAG	3 cut(s)	33, 441, 726
BstDSI	CCRYGG	3 cut(s)	417, 871, 980
BstEII	GGTNACC	1 cut(s)	445
BstF5I	GGATG	2 cut(s)	816, 976
BstFNI	CGCG	1 cut(s)	419
BstKTI	GATC	5 cut(s)	303, 397, 495, 742, 858
BstMAI	GTCTC	2 cut(s)	28, 710
BstMBI	GATC	5 cut(s)	300, 394, 492, 739, 855
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 419, 422, 640, 875, 991
BstNI	CCWGG	1 cut(s)	82
BstNSI	RCATGY	3 cut(s)	296, 994, 1075
BstPI	GGTNACC	1 cut(s)	445
BstSCI	CCNGG	3 cut(s)	80, 100, 520
BstUI	CGCG	1 cut(s)	419
BstV1I	GCAGC	7 cut(s)	16, 66, 97, 376, 400, 572, 1042
BstV2I	GAAGAC	1 cut(s)	1104
BstX2I	RGATCY	2 cut(s)	492, 739
BstXI	CCANNNNNNTGG	1 cut(s)	169
BstYI	RGATCY	2 cut(s)	492, 739
BsuI	GTATCC	1 cut(s)	926
BsuRI	GGCC	2 cut(s)	353, 655
BtgI	CCRYGG	3 cut(s)	417, 871, 980
BtsCI	GGATG	2 cut(s)	816, 976
BtsIMutI	CAGTG	2 cut(s)	922, 943
BveI	ACCTGC	1 cut(s)	523
Cac8I	GCNNGC	6 cut(s)	632, 894, 910, 1136, 1140, 1144
CciI	TCATGA	1 cut(s)	679
Cfr13I	GGNCC	1 cut(s)	254
Cfr42I	CCGCGG	1 cut(s)	420
Csp6I	GTAC	4 cut(s)	606, 1062, 1084, 1150
CspCI	CAANNNNNGTGG	2 cut(s)	853, 888
CviAII	CATG	8 cut(s)	293, 680, 883, 981, 991, 1001, 1006, 1072
CviQI	GTAC	4 cut(s)	606, 1062, 1084, 1150
DdeI	CTNAG	3 cut(s)	33, 441, 726
DpnI	GATC	5 cut(s)	302, 396, 494, 741, 857
DpnII	GATC	5 cut(s)	300, 394, 492, 739, 855
Eam1104I	CTCTTC	1 cut(s)	64
EarI	CTCTTC	1 cut(s)	64
EciI	GGCGGA	1 cut(s)	350
Eco130I	CCWWGG	2 cut(s)	980, 1065
Eco147I	AGGCCT	1 cut(s)	655
Eco47I	GGWCC	1 cut(s)	254
Eco91I	GGTNACC	1 cut(s)	445
EcoO65I	GGTNACC	1 cut(s)	445
EcoRII	CCWGG	1 cut(s)	80
EcoT14I	CCWWGG	2 cut(s)	980, 1065
EcoT22I	ATGCAT	1 cut(s)	294
ErhI	CCWWGG	2 cut(s)	980, 1065
Esp3I	CGTCTC	1 cut(s)	710
FaeI	CATG	8 cut(s)	296, 683, 886, 984, 994, 1004, 1009, 1075
FaqI	GGGAC	2 cut(s)	234, 267
FatI	CATG	8 cut(s)	292, 679, 882, 980, 990, 1000, 1005, 1071
FbaI	TGATCA	2 cut(s)	300, 394
FokI	GGATG	2 cut(s)	823, 963
FspBI	CTAG	1 cut(s)	1139
GsuI	CTGGAG	1 cut(s)	64
HaeIII	GGCC	2 cut(s)	353, 655
HapII	CCGG	4 cut(s)	101, 381, 450, 521
Hin1II	CATG	8 cut(s)	296, 683, 886, 984, 994, 1004, 1009, 1075
HincII	GTYRAC	1 cut(s)	781
HindII	GTYRAC	1 cut(s)	781
HindIII	AAGCTT	1 cut(s)	632
HinfI	GANTC	2 cut(s)	212, 849
HpaII	CCGG	4 cut(s)	101, 381, 450, 521
HphI	GGTGA	3 cut(s)	39, 439, 953
Hpy166II	GTNNAC	3 cut(s)	781, 862, 920
Hpy188I	TCNGA	2 cut(s)	300, 394
Hpy188III	TCNNGA	5 cut(s)	680, 743, 841, 853, 859
Hpy8I	GTNNAC	3 cut(s)	781, 862, 920
HpyAV	CCTTC	4 cut(s)	4, 547, 584, 838
HpyCH4III	ACNGT	8 cut(s)	436, 463, 601, 610, 665, 917, 938, 1044
HpyCH4V	TGCA	5 cut(s)	292, 364, 560, 643, 912
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 419, 422, 640, 875, 991
HpyF3I	CTNAG	3 cut(s)	33, 441, 726
Hsp92II	CATG	8 cut(s)	296, 683, 886, 984, 994, 1004, 1009, 1075
KpnI	GGTACC	1 cut(s)	1065
Ksp22I	TGATCA	2 cut(s)	300, 394
KspI	CCGCGG	1 cut(s)	420
Kzo9I	GATC	5 cut(s)	300, 394, 492, 739, 855
LguI	GCTCTTC	1 cut(s)	64
LmnI	GCTCC	4 cut(s)	93, 105, 614, 970
Lsp1109I	GCAGC	7 cut(s)	16, 66, 97, 376, 400, 572, 1042
LweI	GCATC	4 cut(s)	630, 688, 741, 895
MaeI	CTAG	1 cut(s)	1139
MaeIII	GTNAC	4 cut(s)	235, 328, 445, 986
MalI	GATC	5 cut(s)	302, 396, 494, 741, 857
MboI	GATC	5 cut(s)	300, 394, 492, 739, 855
MboII	GAAGA	5 cut(s)	51, 124, 488, 633, 1109
MfeI	CAATTG	1 cut(s)	1032
MflI	RGATCY	2 cut(s)	492, 739
MluCI	AATT	9 cut(s)	163, 259, 358, 384, 624, 687, 704, 828, 1032
MlyI	GAGTC	1 cut(s)	858
MmeI	TCCRAC	1 cut(s)	592
Mph1103I	ATGCAT	1 cut(s)	294
MroXI	GAANNNNTTC	1 cut(s)	1101
MseI	TTAA	3 cut(s)	345, 540, 690
MslI	CAYNNNNRTG	1 cut(s)	503
MspA1I	CMGCKG	1 cut(s)	419
MspI	CCGG	4 cut(s)	101, 381, 450, 521
MspR9I	CCNGG	3 cut(s)	82, 102, 522
MunI	CAATTG	1 cut(s)	1032
MvaI	CCWGG	1 cut(s)	82
MvnI	CGCG	1 cut(s)	419
MwoI	GCNNNNNNNGC	6 cut(s)	35, 419, 422, 640, 875, 991
NciI	CCSGG	2 cut(s)	102, 522
NcoI	CCATGG	1 cut(s)	980
NdeII	GATC	5 cut(s)	300, 394, 492, 739, 855
NheI	GCTAGC	1 cut(s)	1138
NlaIII	CATG	8 cut(s)	296, 683, 886, 984, 994, 1004, 1009, 1075
NlaIV	GGNNCC	5 cut(s)	255, 494, 953, 1027, 1063
NmuCI	GTSAC	2 cut(s)	328, 445
NsiI	ATGCAT	1 cut(s)	294
NspI	RCATGY	3 cut(s)	296, 994, 1075
PagI	TCATGA	1 cut(s)	679
PceI	AGGCCT	1 cut(s)	655
PciSI	GCTCTTC	1 cut(s)	64
PcsI	WCGNNNNNNNCGW	1 cut(s)	14
PdmI	GAANNNNTTC	1 cut(s)	1101
PfeI	GAWTC	1 cut(s)	212
PleI	GAGTC	1 cut(s)	857
PpsI	GAGTC	1 cut(s)	857
PsiI	TTATAA	1 cut(s)	132
Psp6I	CCWGG	1 cut(s)	80
PspEI	GGTNACC	1 cut(s)	445
PspGI	CCWGG	1 cut(s)	80
PspN4I	GGNNCC	5 cut(s)	255, 494, 953, 1027, 1063
PspPI	GGNCC	1 cut(s)	254
PsuI	RGATCY	2 cut(s)	492, 739
RsaI	GTAC	4 cut(s)	607, 1063, 1085, 1151
RsaNI	GTAC	4 cut(s)	606, 1062, 1084, 1150
RseI	CAYNNNNRTG	1 cut(s)	503
SacII	CCGCGG	1 cut(s)	420
SapI	GCTCTTC	1 cut(s)	64
SaqAI	TTAA	3 cut(s)	345, 540, 690
Sau3AI	GATC	5 cut(s)	300, 394, 492, 739, 855
Sau96I	GGNCC	1 cut(s)	254
SchI	GAGTC	1 cut(s)	858
ScrFI	CCNGG	3 cut(s)	82, 102, 522
SfaNI	GCATC	4 cut(s)	630, 688, 741, 895
Sfr303I	CCGCGG	1 cut(s)	420
SgrBI	CCGCGG	1 cut(s)	420
SinI	GGWCC	1 cut(s)	254
SmiMI	CAYNNNNRTG	1 cut(s)	503
SmlI	CTYRAG	3 cut(s)	269, 407, 839
SmoI	CTYRAG	3 cut(s)	269, 407, 839
Sse9I	AATT	9 cut(s)	163, 259, 358, 384, 624, 687, 704, 828, 1032
SseBI	AGGCCT	1 cut(s)	655
SsiI	CCGC	6 cut(s)	63, 335, 417, 419, 422, 473
SspMI	CTAG	1 cut(s)	1139
StuI	AGGCCT	1 cut(s)	655
StyD4I	CCNGG	3 cut(s)	80, 100, 520
StyI	CCWWGG	2 cut(s)	980, 1065
TaaI	ACNGT	8 cut(s)	436, 463, 601, 610, 665, 917, 938, 1044
TaqI	TCGA	1 cut(s)	1077
TasI	AATT	9 cut(s)	163, 259, 358, 384, 624, 687, 704, 828, 1032
TauI	GCSGC	3 cut(s)	419, 422, 425
TfiI	GAWTC	1 cut(s)	212
Tru1I	TTAA	3 cut(s)	345, 540, 690
Tru9I	TTAA	3 cut(s)	345, 540, 690
TscAI	CASTG	2 cut(s)	922, 943
TseFI	GTSAC	2 cut(s)	328, 445
TseI	GCWGC	7 cut(s)	29, 54, 85, 364, 413, 560, 1055
Tsp45I	GTSAC	2 cut(s)	328, 445
TspRI	CASTG	2 cut(s)	922, 943
VpaK11BI	GGWCC	1 cut(s)	254
XapI	RAATTY	3 cut(s)	358, 704, 828
XceI	RCATGY	3 cut(s)	296, 994, 1075
XmnI	GAANNNNTTC	1 cut(s)	1101
XspI	CTAG	1 cut(s)	1139
Zsp2I	ATGCAT	1 cut(s)	294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.