Rh3DG333800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016997
Physical Location & Seq
Forward (+)
37142220 .. 37143042
823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG333800.1

Sequence Viewer

Length: 555 bp
ATGGAGGTCCAAACAGTTGACCGGCCACCAGCACAGCACCCTGTTGAACAATGTAGCATTATGCAGGTGTCGAGAGTGGAAGAAACAGGAGGACATCAAGAAATAATTAGAGAAGAAGAGGAGGTGCAAGCTATCGAAGAGGAGGTGCAAGCTATTGAAGAGCAGCCTTCACCACCGGCTGCTGAACGATGTAGCATTGGACATGCATGGAGTGAGGAAGAAGTTGGAGCACTCAACACAGGATCGGGTAGTGAGGATGACTTCCAGATCATTGAGGTGAAGCAATCACAATCCCCAGTGGCTGCAGGCGCTCTATCCAAGCTTTGGGGTTTCTGGAAAGTTATCTCGGCCATCACGAAGAAAGCTTGGCCAGCACCAATGAAGCTTCAATCCCCAATCTGGATGAGGTTGCTCGAGCTAAGGCCAACATCCAAAAGATTTCCACTCTCTCTATTGATGAAGTCATGGAATCTGAGTGTGTTGGACGCTTCAAATCATCGTTGGAATTTTTGCTCAAAGCTGATGACAGAGCTGGAGCTCAGTCGGCAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.79

Weight (kDa)

5.01

Isoelectric Point (pI)

76.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 55
Acc36I ACCTGC 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 324
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 3 cut(s) 23, 348, 368
AcsI RAATTY 1 cut(s) 505
AfiI CCNNNNNNNGG 2 cut(s) 324, 399
AgsI TTSAA 4 cut(s) 47, 158, 389, 492
AluBI AGCT 9 cut(s) 131, 152, 322, 365, 385, 418, 520, 532, 538
AluI AGCT 9 cut(s) 131, 152, 322, 365, 385, 418, 520, 532, 538
Alw21I GWGCWC 2 cut(s) 232, 540
AlwI GGATC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 4 cut(s) 23, 348, 368, 422
ApeKI GCWGC 3 cut(s) 163, 179, 302
ApoI RAATTY 1 cut(s) 505
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 162, 289
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 370
BanII GRGCYC 1 cut(s) 540
Bbv12I GWGCWC 2 cut(s) 232, 540
BbvI GCAGC 3 cut(s) 166, 175, 289
BccI CCATC 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 55
BisI GCNGC 3 cut(s) 164, 180, 303
BlsI GCNGC 3 cut(s) 165, 181, 304
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 290
BmuI ACTGGG 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 554
Bpu10I CCTNAGC 1 cut(s) 419
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 399
Bse118I RCCGGY 2 cut(s) 21, 175
Bse1I ACTGG 1 cut(s) 296
BseGI GGATG 3 cut(s) 262, 408, 428
BseLI CCNNNNNNNGG 2 cut(s) 324, 399
BseMII CTCAG 2 cut(s) 464, 553
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 2 cut(s) 134, 155
BseXI GCAGC 3 cut(s) 166, 175, 289
BshFI GGCC 4 cut(s) 25, 350, 370, 424
BsiHKAI GWGCWC 2 cut(s) 232, 540
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 2 cut(s) 22, 176
BslI CCNNNNNNNGG 2 cut(s) 324, 399
BsnI GGCC 4 cut(s) 25, 350, 370, 424
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 2 cut(s) 232, 540
Bsp143I GATC 2 cut(s) 242, 267
BspANI GGCC 4 cut(s) 25, 350, 370, 424
BspCNI CTCAG 2 cut(s) 465, 552
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 55
BspPI GGATC 1 cut(s) 250
BspQI GCTCTTC 1 cut(s) 153
BsrFI RCCGGY 2 cut(s) 21, 175
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 2 cut(s) 21, 175
BssMI GATC 2 cut(s) 242, 267
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 111, 132, 153
BstC8I GCNNGC 4 cut(s) 129, 150, 307, 372
BstDEI CTNAG 3 cut(s) 419, 473, 539
BstF5I GGATG 3 cut(s) 262, 408, 428
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 2 cut(s) 245, 270
BstMBI GATC 2 cut(s) 242, 267
BstMWI GCNNNNNNNGC 3 cut(s) 308, 371, 544
BstNSI RCATGY 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 3 cut(s) 166, 175, 289
BsuRI GGCC 4 cut(s) 25, 350, 370, 424
BtsCI GGATG 3 cut(s) 262, 408, 428
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 55
Cac8I GCNNGC 4 cut(s) 129, 150, 307, 372
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 2 cut(s) 21, 175
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 494
CviAII CATG 3 cut(s) 203, 207, 465
DdeI CTNAG 3 cut(s) 419, 473, 539
DpnI GATC 2 cut(s) 244, 269
DpnII GATC 2 cut(s) 242, 267
EaeI YGGCCR 3 cut(s) 23, 348, 368
Eam1104I CTCTTC 3 cut(s) 111, 132, 153
EarI CTCTTC 3 cut(s) 111, 132, 153
Ecl136II GAGCTC 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 538
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 538
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 540
FaeI CATG 3 cut(s) 206, 210, 468
FaiI YATR 5 cut(s) 62, 204, 208, 466, 553
FatI CATG 3 cut(s) 202, 206, 464
Fnu4HI GCNGC 3 cut(s) 164, 180, 303
FokI GGATG 3 cut(s) 269, 415, 415
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 3 cut(s) 164, 180, 303
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 164, 180, 303
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 4 cut(s) 25, 350, 370, 424
HapII CCGG 2 cut(s) 22, 176
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 206, 210, 468
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 3 cut(s) 320, 363, 383
HinfI GANTC 1 cut(s) 469
HpaII CCGG 2 cut(s) 22, 176
HphI GGTGA 2 cut(s) 162, 289
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 6 cut(s) 72, 98, 265, 334, 355, 400
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 5 cut(s) 64, 127, 148, 206, 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 371, 544
HpyF3I CTNAG 3 cut(s) 419, 473, 539
Hsp92II CATG 3 cut(s) 206, 210, 468
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 2 cut(s) 242, 267
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 227, 535
Lsp1109I GCAGC 3 cut(s) 166, 175, 289
MalI GATC 2 cut(s) 244, 269
MboI GATC 2 cut(s) 242, 267
MboII GAAGA 7 cut(s) 92, 125, 128, 149, 170, 230, 370
MhlI GDGCHC 2 cut(s) 232, 540
MlsI TGGCCA 1 cut(s) 370
MluCI AATT 2 cut(s) 105, 505
MluNI TGGCCA 1 cut(s) 370
MmeI TCCRAC 3 cut(s) 205, 462, 482
MnlI CCTC 9 cut(s) 83, 112, 115, 133, 136, 208, 247, 268, 399
Mox20I TGGCCA 1 cut(s) 370
Mph1103I ATGCAT 1 cut(s) 208
MscI TGGCCA 1 cut(s) 370
MslI CAYNNNNRTG 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 370
MspI CCGG 2 cut(s) 22, 176
MwoI GCNNNNNNNGC 3 cut(s) 308, 371, 544
NdeII GATC 2 cut(s) 242, 267
NlaIII CATG 3 cut(s) 206, 210, 468
NmeAIII GCCGAG 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 206
PaeR7I CTCGAG 1 cut(s) 413
PaqCI CACCTGC 1 cut(s) 55
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PfeI GAWTC 1 cut(s) 469
PflMI CCANNNNNTGG 1 cut(s) 324
PkrI GCNGC 3 cut(s) 165, 181, 304
Psp124BI GAGCTC 1 cut(s) 540
PspPI GGNCC 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 413
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 540
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 3 cut(s) 164, 180, 303
Sau3AI GATC 2 cut(s) 242, 267
Sau96I GGNCC 1 cut(s) 7
SduI GDGCHC 2 cut(s) 232, 540
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 2 cut(s) 105, 505
SstI GAGCTC 1 cut(s) 540
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 3 cut(s) 71, 135, 414
TasI AATT 2 cut(s) 105, 505
TfiI GAWTC 1 cut(s) 469
TscAI CASTG 1 cut(s) 303
TseI GCWGC 3 cut(s) 163, 179, 302
TspDTI ATGAA 2 cut(s) 395, 473
TspRI CASTG 1 cut(s) 303
Van91I CCANNNNNTGG 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 505
XceI RCATGY 1 cut(s) 206
XhoI CTCGAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.