Rh4AG373200

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016837
Physical Location & Seq
Reverse (-)
68100560 .. 68102755
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG373200.1

Sequence Viewer

Length: 1374 bp
ATGGATCAGGAAAGAAGTAGTAGCTGTGATATGAGTGCAGCAGATCAAGACAAAATTGATGAAGTGCTGCTGCCAGGGTTTCGTTTTCACCCAACAGATGAGGAGCTTGTTGGGTTTTATCTCAAAAGGAAGATCCAGCAACGCCCTCTCTCGATCGAGCTCATCAAGCAACTCGACATCTATAAATTCGATCCATGGGATCTTCCAAAGTTGGCAGCTAGTGGGGAGAAGGAGTGGTATTTCTACTGCCCGAGGGACAGAAAATACAGAAACAGCACGAGGCCTAACAGGGTCACCGGAGCCGGGTTCTGGAAAGCCACGGGTACCGACCGGCCCATTTACTCGTCGGAAGGTAACTCCAACAAGTGTATTGGGTTGAAGAAGTCCCTTGTTTTTTACAGAGGTAGAGCTGCCAAAGGGGTCAAAACCGACTGGATGATGCATGAGTTTCGCCTACCTTCTCTCACCGACTCCTCAGTACTCCCACCAAAGAGATCGTTCATGGACAAAATTGCCATTCCCGCAAATGACTCATGGGCAATATGCAGGATATTCAAAAAAACAAATTCTAGTGCCCATCAAAGAGCCATTTCTCATCATTCTTGGGTGTCTCAGACCTTGTCTGAACCAAACACATTTCAAGATCATGTGTTAGGCAGCTCCAGATTAGGTGCACAAACCACTAGCACTACTCAGTACGGTGGTAACAATATTGACACACAACAGATCTCAACTTCCACCTCAACCAGTACTCTTTCCCCAGGAGATTATTATGCTAACAATTCCTACAAAACCTTACACCCATCAATCGCTTGCAATCTTAAACCCTATCATCAGTACTTCCCCATTTCCAATGCCGGAGAGTACTGTAACCCTAATTTCACATTTTCACCTCTGGAGAGTCCGGCAGCCAAATGTTCAGTGGATGTTTCGTCCTTGTTGTTAAACATGTCGTCATCGGTCCTTGGAGATTTCGGAACCAAGCTGGCCTCGGCTGAGAGTAGTACTACTTTCGACTTTAGCAGTACTACTGGTTCTCAGGAGCAACAGTGTAATAATGGCTTCTCCTCCTTGACGACATTACCACACCATGACGTGATGCAAGGGAACCAGCTTGGCGTTGTTGGGAGTCATGATCACAGCATCGATGCGTTGATGAAGAATATAACGAATCATGCGAACTCAAACGCGATAGAAACAGATGATCATGGACACTGGGAGAGTAGTCTGCGATCATCGATTGGATTTCCTTTCATGAATAGTTTACCAGTAATACCTTTGAATATAGGAGGTGGGGATGCATGGAAGTCCAACTTGACGTGGGACTCTTCACCATGTCCGAGTGAAATGTCCACAACCAGATGCTATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

457

Amino Acids

50.76

Weight (kDa)

6.73

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 25 - 152 1.1e-47 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016837)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31420
malus_domestica MD13G1072500.v1.1 MD16G1073300.v1.1
prunus_persica Prupe.1G274300_v2.0.a1
pyrus_communis pycom13g06500
rosa_chinensis RchiOBHm_Chr4g0439901
rosa_laevigata RLG00000006228
rosa_multiflora Rmu_sc0000497.1_g000002
rosa_roxburghii Rroxscaffold_5G00380850
rosa_rugosa Rorug04G0322200
rosa_samantha Rh4AG373200 Rh4CG400000 Rh4DG379800
rosa_wichuraiana Rw4G031910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 323
AccII CGCG 1 cut(s) 1190
AciI CCGC 1 cut(s) 522
AclWI GGATC 4 cut(s) 12, 127, 185, 207
AcsI RAATTY 2 cut(s) 185, 565
AfaI GTAC 8 cut(s) 325, 480, 698, 751, 839, 866, 1006, 1027
AfiI CCNNNNNNNGG 2 cut(s) 303, 309
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 4 cut(s) 379, 556, 641, 1282
AjiI CACGTC 2 cut(s) 1096, 1320
AjnI CCWGG 2 cut(s) 73, 760
AluBI AGCT 8 cut(s) 24, 106, 160, 218, 410, 660, 985, 1114
AluI AGCT 8 cut(s) 24, 106, 160, 218, 410, 660, 985, 1114
Alw21I GWGCWC 2 cut(s) 162, 676
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 672
AlwI GGATC 4 cut(s) 12, 127, 185, 207
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 3 cut(s) 281, 332, 987
ApaLI GTGCAC 1 cut(s) 672
ApeKI GCWGC 7 cut(s) 38, 67, 70, 215, 410, 657, 908
ApoI RAATTY 2 cut(s) 185, 565
Asp718I GGTACC 1 cut(s) 323
AspS9I GGNCC 2 cut(s) 333, 961
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 5 cut(s) 80, 286, 457, 882, 1323
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 1 cut(s) 961
BaeGI GKGCMC 2 cut(s) 577, 676
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 162
BauI CACGAG 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 162, 676
BbvI GCAGC 7 cut(s) 50, 54, 57, 227, 397, 669, 920
BccI CCATC 2 cut(s) 585, 811
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 75, 762
BclI TGATCA 2 cut(s) 1135, 1204
BcnI CCSGG 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 3 cut(s) 219, 570, 684
BglII AGATCT 1 cut(s) 726
BisI GCNGC 7 cut(s) 39, 68, 71, 216, 411, 658, 909
BlsI GCNGC 7 cut(s) 40, 69, 72, 217, 412, 659, 910
BmcAI AGTACT 6 cut(s) 480, 751, 839, 866, 1006, 1027
Bme1390I CCNGG 3 cut(s) 75, 304, 762
Bme18I GGWCC 1 cut(s) 961
BmeT110I CYCGRG 1 cut(s) 250
BmgBI CACGTC 2 cut(s) 1096, 1320
BmgT120I GGNCC 2 cut(s) 333, 961
BmiI GGNNCC 4 cut(s) 301, 325, 979, 1109
BmrFI CCNGG 3 cut(s) 75, 304, 762
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 6 cut(s) 429, 1089, 1138, 1152, 1288, 1352
BmuI ACTGGG 1 cut(s) 1225
BpmI CTGGAG 2 cut(s) 646, 917
BpuMI CCSGG 1 cut(s) 304
Bsa29I ATCGAT 2 cut(s) 1146, 1238
BsaJI CCNNGG 7 cut(s) 74, 194, 251, 318, 760, 964, 990
BsaWI WCCGGW 1 cut(s) 296
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 1034, 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 309
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 5 cut(s) 437, 747, 1036, 1220, 1268
BseBI CCWGG 2 cut(s) 75, 762
BseCI ATCGAT 2 cut(s) 1146, 1238
BseDI CCNNGG 7 cut(s) 74, 194, 251, 318, 760, 964, 990
BseGI GGATG 3 cut(s) 441, 931, 1303
BseLI CCNNNNNNNGG 2 cut(s) 303, 309
BseMII CTCAG 5 cut(s) 489, 626, 707, 987, 1052
BseNI ACTGG 5 cut(s) 437, 747, 1036, 1220, 1268
BseRI GAGGAG 3 cut(s) 116, 463, 1057
BseSI GKGCMC 2 cut(s) 577, 676
BseXI GCAGC 7 cut(s) 50, 54, 57, 227, 397, 669, 920
BsgI GTGCAG 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 1190
Bsh1285I CGRYCG 2 cut(s) 156, 331
BshFI GGCC 3 cut(s) 283, 334, 989
BshNI GGYRCC 1 cut(s) 323
BshVI ATCGAT 2 cut(s) 1146, 1238
BsiEI CGRYCG 2 cut(s) 156, 331
BsiHKAI GWGCWC 2 cut(s) 162, 676
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 5 cut(s) 297, 303, 331, 858, 905
BslFI GGGAC 3 cut(s) 269, 370, 1337
BslI CCNNNNNNNGG 2 cut(s) 303, 309
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 3 cut(s) 269, 370, 1337
BsnI GGCC 3 cut(s) 283, 334, 989
BsoBI CYCGRG 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 162, 577, 676
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 1 cut(s) 522
BspANI GGCC 3 cut(s) 283, 334, 989
BspCNI CTCAG 5 cut(s) 488, 625, 706, 988, 1051
BspDI ATCGAT 2 cut(s) 1146, 1238
BspFNI CGCG 1 cut(s) 1190
BspHI TCATGA 2 cut(s) 1132, 1254
BspLI GGNNCC 4 cut(s) 301, 325, 979, 1109
BspPI GGATC 4 cut(s) 12, 127, 185, 207
BspT107I GGYRCC 1 cut(s) 323
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 5 cut(s) 437, 747, 1036, 1220, 1268
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 7 cut(s) 74, 194, 251, 318, 760, 964, 990
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 2 cut(s) 194, 964
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 75, 762
Bst4CI ACNGT 3 cut(s) 701, 869, 1050
Bst6I CTCTTC 1 cut(s) 1333
BstC8I GCNNGC 2 cut(s) 814, 987
BstDEI CTNAG 6 cut(s) 475, 612, 693, 996, 1038, 1371
BstDSI CCRYGG 2 cut(s) 194, 318
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 3 cut(s) 441, 931, 1303
BstFNI CGCG 1 cut(s) 1190
BstMAI GTCTC 1 cut(s) 615
BstMCI CGRYCG 2 cut(s) 156, 331
BstMWI GCNNNNNNNGC 2 cut(s) 166, 521
BstNI CCWGG 2 cut(s) 75, 762
BstNSI RCATGY 1 cut(s) 952
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 73, 302, 760
BstSLI GKGCMC 2 cut(s) 577, 676
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 7 cut(s) 50, 54, 57, 227, 397, 669, 920
BstX2I RGATCY 3 cut(s) 132, 199, 726
BstYI RGATCY 3 cut(s) 132, 199, 726
Bsu15I ATCGAT 2 cut(s) 1146, 1238
BsuRI GGCC 3 cut(s) 283, 334, 989
BsuTUI ATCGAT 2 cut(s) 1146, 1238
BtgI CCRYGG 2 cut(s) 194, 318
BtrI CACGTC 2 cut(s) 1096, 1320
BtsCI GGATG 3 cut(s) 441, 931, 1303
BtsIMutI CAGTG 3 cut(s) 927, 1055, 1213
Cac8I GCNNGC 2 cut(s) 814, 987
CciI TCATGA 2 cut(s) 1132, 1254
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 333, 961
ClaI ATCGAT 2 cut(s) 1146, 1238
Csp6I GTAC 8 cut(s) 324, 479, 697, 750, 838, 865, 1005, 1026
CviQI GTAC 8 cut(s) 324, 479, 697, 750, 838, 865, 1005, 1026
DdeI CTNAG 6 cut(s) 475, 612, 693, 996, 1038, 1371
Eam1104I CTCTTC 1 cut(s) 1333
EarI CTCTTC 1 cut(s) 1333
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 194, 964
Eco147I AGGCCT 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 961
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 250
Eco91I GGTNACC 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 73, 760
EcoT14I CCWWGG 2 cut(s) 194, 964
EcoT22I ATGCAT 2 cut(s) 444, 1303
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 2 cut(s) 194, 964
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 3 cut(s) 269, 370, 1337
FauI CCCGC 1 cut(s) 529
FbaI TGATCA 2 cut(s) 1135, 1204
Fnu4HI GCNGC 7 cut(s) 39, 68, 71, 216, 411, 658, 909
FokI GGATG 3 cut(s) 448, 938, 1310
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 7 cut(s) 39, 68, 71, 216, 411, 658, 909
FspBI CTAG 3 cut(s) 219, 570, 684
GluI GCNGC 7 cut(s) 39, 68, 71, 216, 411, 658, 909
GsuI CTGGAG 2 cut(s) 646, 917
HaeIII GGCC 3 cut(s) 283, 334, 989
HapII CCGG 5 cut(s) 297, 303, 331, 858, 905
HinfI GANTC 6 cut(s) 470, 530, 901, 1129, 1171, 1325
HpaII CCGG 5 cut(s) 297, 303, 331, 858, 905
HphI GGTGA 5 cut(s) 80, 286, 457, 882, 1323
Hpy166II GTNNAC 3 cut(s) 674, 1265, 1353
Hpy188I TCNGA 5 cut(s) 349, 615, 625, 977, 1341
Hpy8I GTNNAC 3 cut(s) 674, 1265, 1353
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 3 cut(s) 223, 344, 468
HpyCH4III ACNGT 3 cut(s) 701, 869, 1050
HpyCH4IV ACGT 2 cut(s) 1095, 1319
HpyCH4V TGCA 7 cut(s) 38, 442, 546, 674, 816, 1102, 1301
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 521
HpyF3I CTNAG 6 cut(s) 475, 612, 693, 996, 1038, 1371
HpySE526I ACGT 2 cut(s) 1095, 1319
KpnI GGTACC 1 cut(s) 327
Ksp22I TGATCA 2 cut(s) 1135, 1204
LmnI GCTCC 4 cut(s) 103, 299, 665, 1042
Lsp1109I GCAGC 7 cut(s) 50, 54, 57, 227, 397, 669, 920
LweI GCATC 6 cut(s) 429, 1089, 1138, 1152, 1288, 1352
MaeI CTAG 3 cut(s) 219, 570, 684
MaeII ACGT 2 cut(s) 1095, 1319
MaeIII GTNAC 4 cut(s) 292, 353, 704, 869
MboII GAAGA 5 cut(s) 142, 194, 391, 1171, 1320
MflI RGATCY 3 cut(s) 132, 199, 726
MhlI GDGCHC 3 cut(s) 162, 577, 676
MluCI AATT 6 cut(s) 54, 185, 510, 565, 781, 877
MlyI GAGTC 5 cut(s) 464, 524, 910, 1138, 1319
MmeI TCCRAC 3 cut(s) 327, 384, 1335
Mph1103I ATGCAT 2 cut(s) 444, 1303
MseI TTAA 2 cut(s) 822, 944
MspI CCGG 5 cut(s) 297, 303, 331, 858, 905
MspR9I CCNGG 3 cut(s) 75, 304, 762
MvaI CCWGG 2 cut(s) 75, 762
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 2 cut(s) 166, 521
NciI CCSGG 1 cut(s) 304
NcoI CCATGG 1 cut(s) 194
NlaIV GGNNCC 4 cut(s) 301, 325, 979, 1109
NmeAIII GCCGAG 1 cut(s) 971
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 2 cut(s) 444, 1303
NspI RCATGY 1 cut(s) 952
PagI TCATGA 2 cut(s) 1132, 1254
PceI AGGCCT 1 cut(s) 283
PciI ACATGT 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 1175
PfeI GAWTC 1 cut(s) 1171
PflFI GACNNNGTC 1 cut(s) 619
PkrI GCNGC 7 cut(s) 40, 69, 72, 217, 412, 659, 910
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 5 cut(s) 464, 524, 909, 1137, 1319
PpsI GAGTC 5 cut(s) 464, 524, 909, 1137, 1319
PscI ACATGT 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 73, 760
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 73, 760
PspN4I GGNNCC 4 cut(s) 301, 325, 979, 1109
PspPI GGNCC 2 cut(s) 333, 961
PsrI GAACNNNNNNTAC 2 cut(s) 1018, 1050
PsuI RGATCY 3 cut(s) 132, 199, 726
PsyI GACNNNGTC 1 cut(s) 619
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 8 cut(s) 325, 480, 698, 751, 839, 866, 1006, 1027
RsaNI GTAC 8 cut(s) 324, 479, 697, 750, 838, 865, 1005, 1026
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 822, 944
SatI GCNGC 7 cut(s) 39, 68, 71, 216, 411, 658, 909
Sau96I GGNCC 2 cut(s) 333, 961
ScaI AGTACT 6 cut(s) 480, 751, 839, 866, 1006, 1027
SchI GAGTC 5 cut(s) 464, 524, 910, 1138, 1319
ScrFI CCNGG 3 cut(s) 75, 304, 762
SduI GDGCHC 3 cut(s) 162, 577, 676
SfaNI GCATC 6 cut(s) 429, 1089, 1138, 1152, 1288, 1352
SinI GGWCC 1 cut(s) 961
Sse9I AATT 6 cut(s) 54, 185, 510, 565, 781, 877
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 1 cut(s) 522
SspI AATATT 1 cut(s) 712
SspMI CTAG 3 cut(s) 219, 570, 684
SstI GAGCTC 1 cut(s) 162
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 3 cut(s) 73, 302, 760
StyI CCWWGG 2 cut(s) 194, 964
TaaI ACNGT 3 cut(s) 701, 869, 1050
TaiI ACGT 2 cut(s) 1098, 1322
TaqI TCGA 7 cut(s) 152, 156, 174, 189, 1014, 1146, 1238
TaqII GACCGA 1 cut(s) 949
TasI AATT 6 cut(s) 54, 185, 510, 565, 781, 877
TatI WGTACW 6 cut(s) 478, 749, 837, 864, 1004, 1025
TfiI GAWTC 1 cut(s) 1171
Tru1I TTAA 2 cut(s) 822, 944
Tru9I TTAA 2 cut(s) 822, 944
TscAI CASTG 3 cut(s) 927, 1055, 1220
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 7 cut(s) 38, 67, 70, 215, 410, 657, 908
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 5 cut(s) 75, 490, 1172, 1243, 1271
TspRI CASTG 3 cut(s) 927, 1055, 1220
Tth111I GACNNNGTC 1 cut(s) 619
VneI GTGCAC 1 cut(s) 672
VpaK11BI GGWCC 1 cut(s) 961
XapI RAATTY 2 cut(s) 185, 565
XceI RCATGY 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XspI CTAG 3 cut(s) 219, 570, 684
ZrmI AGTACT 6 cut(s) 480, 751, 839, 866, 1006, 1027
Zsp2I ATGCAT 2 cut(s) 444, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.