Rh4BG083400

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015593
Physical Location & Seq
Forward (+)
14467915 .. 14469587
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG083400.1

Sequence Viewer

Length: 687 bp
ATGGGTGATTATGATCCTGAAGCATGCAACACAAGCCTCCAACTTGGACTTGGTTTGGGTGGATATGCTCCAAGGCAAGAGATGAGGACCAAGGATAGGCCTCTGGTCTGCTTAGACCTATCATTTGCTCTTCGCCCAAAACCGGAGAAGATTGCTAACTTGGATGATCACATGGCAAATGGATCGAGTTTGAAGACGAATATTGTCTATGAAGACGAAGAAGATCGGAGAAGCACTGATCATACCGATAGCGAGATCACCAACAGCAAAAATGGTGCAAGAAAGAAACTGAGGCTGACAAAAGAGCAATCCACTTTGCTAGAAGACAGCTTCAAGCGCCATAGCACCCTTAATCCGACCCAGAAACATGCTCTTGCTGGGCTATTGAATCTGAAGCCAAGGCAGGTTGAAGTTTGGTTCCAGAACAGAAGAGCAAGGACAAAGCTGAAACAAACAGAAGTGGACTGTGAGTTCTTGAAGAAATGGTGCGAAAGTCTGAGCGACGAGAATCGAAAACTGAAGAAGGAACTGCAGGAGCTAAAATCGTTGAGTGTGAATGGGGCATCATCACCATTATTGACTCATCAAATCCCAAAGACCACGATGCTCACAATGTGTTCATCTTGTGAGAGAAAGGCAAAAGCTAATGATCAATATCCTAATAACGAAGCAGCAGCAAAGAATTAA

Protein Analysis

228

Amino Acids

25.88

Weight (kDa)

8.95

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 94 - 148 4.7e-15 Homeodomain
HALZ PF02183 150 - 183 4.5e-10 Homeobox associated leucine zipper
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06590
malus_domestica MD13G1236500.v1.1 MD16G1241700.v1.1
prunus_persica Prupe.1G068400_v2.0.a1
pyrus_communis pycom13g20920 pycom16g20240
rosa_chinensis RchiOBHm_Chr4g0398491
rosa_laevigata RLG00000009359
rosa_multiflora Rmu_ssc0000240.1_g000024
rosa_roxburghii Rroxscaffold_5G00343460
rosa_rugosa Rorug04G0008800
rosa_samantha Rh4AG086800 Rh4BG083400 Rh4CG094800 Rh4DG078200
rosa_wichuraiana Rw4G007230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
AclWI GGATC 2 cut(s) 8, 190
AcuI CTGAAG 3 cut(s) 39, 413, 539
AdeI CACNNNGTG 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 96, 142
AgsI TTSAA 5 cut(s) 193, 334, 388, 410, 478
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 4 cut(s) 330, 445, 538, 644
AluI AGCT 4 cut(s) 330, 445, 538, 644
AlwI GGATC 2 cut(s) 8, 190
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 671, 674
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 17, 250, 561
AvaII GGWCC 1 cut(s) 87
BbsI GAAGAC 3 cut(s) 200, 219, 330
BbvI GCAGC 1 cut(s) 683
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BclI TGATCA 3 cut(s) 166, 238, 649
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 340
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 2 cut(s) 672, 675
BlsI GCNGC 2 cut(s) 673, 676
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 419
BmsI GCATC 2 cut(s) 572, 594
BpiI GAAGAC 3 cut(s) 200, 219, 330
BsaBI GATNNNNATC 2 cut(s) 12, 654
BsaJI CCNNGG 3 cut(s) 71, 90, 398
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 142
Bse8I GATNNNNATC 2 cut(s) 12, 654
BseDI CCNNGG 3 cut(s) 71, 90, 398
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 2 cut(s) 12, 654
BseLI CCNNNNNNNGG 2 cut(s) 96, 142
BseMII CTCAG 2 cut(s) 281, 488
BseXI GCAGC 1 cut(s) 683
BseYI CCCAGC 1 cut(s) 377
BshFI GGCC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 96, 142
BsnI GGCC 1 cut(s) 100
Bsp143I GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 2 cut(s) 282, 489
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 534
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 2 cut(s) 8, 190
BspQI GCTCTTC 2 cut(s) 135, 424
BssECI CCNNGG 3 cut(s) 71, 90, 398
BssMI GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
BssT1I CCWWGG 3 cut(s) 71, 90, 398
Bst4CI ACNGT 1 cut(s) 467
Bst6I CTCTTC 2 cut(s) 135, 424
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 3 cut(s) 112, 290, 497
BstF5I GGATG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 7 cut(s) 16, 169, 185, 226, 241, 258, 652
BstMBI GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
BstMWI GCNNNNNNNGC 2 cut(s) 33, 336
BstNSI RCATGY 2 cut(s) 27, 371
BstSFI CTRYAG 1 cut(s) 530
BstV1I GCAGC 1 cut(s) 683
BstV2I GAAGAC 3 cut(s) 200, 219, 330
BsuRI GGCC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 87
CviAII CATG 3 cut(s) 24, 172, 368
CviJI RGCY 9 cut(s) 36, 100, 295, 330, 382, 397, 445, 538, 644
CviKI_1 RGCY 9 cut(s) 36, 100, 295, 330, 382, 397, 445, 538, 644
DdeI CTNAG 3 cut(s) 112, 290, 497
DpnI GATC 7 cut(s) 15, 168, 184, 225, 240, 257, 651
DpnII GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
DraIII CACNNNGTG 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 135, 424
EarI CTCTTC 2 cut(s) 135, 424
Eco130I CCWWGG 3 cut(s) 71, 90, 398
Eco147I AGGCCT 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 3 cut(s) 39, 413, 539
EcoT14I CCWWGG 3 cut(s) 71, 90, 398
ErhI CCWWGG 3 cut(s) 71, 90, 398
FaeI CATG 3 cut(s) 27, 175, 371
FaiI YATR 8 cut(s) 12, 25, 66, 173, 210, 243, 342, 369
FatI CATG 3 cut(s) 23, 171, 367
FbaI TGATCA 3 cut(s) 166, 238, 649
Fnu4HI GCNGC 2 cut(s) 672, 675
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 672, 675
FspBI CTAG 1 cut(s) 320
GlaI GCGC 1 cut(s) 338
GluI GCNGC 2 cut(s) 672, 675
GsaI CCCAGC 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 1 cut(s) 100
HapII CCGG 1 cut(s) 143
HhaI GCGC 1 cut(s) 339
Hin1II CATG 3 cut(s) 27, 175, 371
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HinfI GANTC 3 cut(s) 388, 508, 580
HpaII CCGG 1 cut(s) 143
HphI GGTGA 3 cut(s) 17, 250, 561
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 4 cut(s) 228, 357, 393, 498
Hpy188III TCNNGA 3 cut(s) 17, 421, 475
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4V TGCA 3 cut(s) 27, 278, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 336
HpyF3I CTNAG 3 cut(s) 112, 290, 497
Hsp92II CATG 3 cut(s) 27, 175, 371
HspAI GCGC 1 cut(s) 337
Ksp22I TGATCA 3 cut(s) 166, 238, 649
Kzo9I GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
LguI GCTCTTC 2 cut(s) 135, 424
LmnI GCTCC 2 cut(s) 73, 535
LpnPI CCDG 8 cut(s) 30, 89, 156, 363, 374, 389, 434, 518
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 2 cut(s) 572, 594
MaeI CTAG 1 cut(s) 320
MalI GATC 7 cut(s) 15, 168, 184, 225, 240, 257, 651
MboI GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
MluCI AATT 1 cut(s) 682
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 2 cut(s) 64, 380
MnlI CCTC 4 cut(s) 47, 78, 111, 285
MseI TTAA 2 cut(s) 351, 685
MspI CCGG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 33, 336
NdeII GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
NlaIII CATG 3 cut(s) 27, 175, 371
NlaIV GGNNCC 1 cut(s) 419
NspI RCATGY 2 cut(s) 27, 371
PaeI GCATGC 1 cut(s) 27
PceI AGGCCT 1 cut(s) 100
PciSI GCTCTTC 2 cut(s) 135, 424
PfeI GAWTC 2 cut(s) 388, 508
PkrI GCNGC 2 cut(s) 673, 676
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PspFI CCCAGC 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 87
PstI CTGCAG 1 cut(s) 534
SapI GCTCTTC 2 cut(s) 135, 424
SaqAI TTAA 2 cut(s) 351, 685
SatI GCNGC 2 cut(s) 672, 675
Sau3AI GATC 7 cut(s) 13, 166, 182, 223, 238, 255, 649
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 574
SetI ASST 6 cut(s) 120, 332, 408, 447, 540, 646
SfaNI GCATC 2 cut(s) 572, 594
SfcI CTRYAG 1 cut(s) 530
SinI GGWCC 1 cut(s) 87
SphI GCATGC 1 cut(s) 27
Sse9I AATT 1 cut(s) 682
SseBI AGGCCT 1 cut(s) 100
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 320
StuI AGGCCT 1 cut(s) 100
StyI CCWWGG 3 cut(s) 71, 90, 398
TaaI ACNGT 1 cut(s) 467
TaqI TCGA 2 cut(s) 185, 511
TasI AATT 1 cut(s) 682
TfiI GAWTC 2 cut(s) 388, 508
Tru1I TTAA 2 cut(s) 351, 685
Tru9I TTAA 2 cut(s) 351, 685
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 671, 674
TspDTI ATGAA 2 cut(s) 225, 609
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 87
XceI RCATGY 2 cut(s) 27, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.