Rh4BG102300

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014712
Physical Location & Seq
Reverse (-)
17676270 .. 17678246
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG102300.1

Sequence Viewer

Length: 726 bp
ATGGGATCAATGATCAAATACCTTTCAGGAAATTGCGCCCACTGTCTTCTAATACCGCTCTTAACAAACTGTGCATCACCCTTTCAAACACAGAGACTGGTTTGTTTGTTTCTCCAACCAATTCTACATTTCTACCACCTTTACATAAACCTTTTGCTCTGTGTTTCTTTACAAACCACACTGTTCTTTGTTAAACAGAAGATGTCTGCTGCTATCTTCAATTTTCCGGTTGCTGCATCTGGTTATGTTGGCTTGGCAACCAACCCTTCAAAAATTTTCCCAGCTAAAGACTCTATCGGATGGACCAAGAAAACCGTCTCCAATGGTTCAAGAACTTGCTGCATGAAGACGTGGAACCCGATCAACAACAAGAAATTTGAGACTCTGTCTTACCTCCCACCACTCTCTGATGATTCAATTGCAAAGGAGATTGACTACATGCTCAAGAAGGGATGGATCCCTTGCCTTGAATTTGATGAGGTGGGTTATGTTCGTAGGGAGAATAGCAGGATGCCAGGGTACTATGATGGAAGATACTGGACATTGTGGAAGCTACCCATGTTTGGTTGTACCGACCCTTCTCTGGTGCTCAATGAAATCCAAGAGTGCAAAAAAACGTACCCAAATGCTTATATACGCTGTTTAGCATTTGACAACCAGAAGCAGGGTCAGTGCATGGCCTTTATCATTCAGAAGCCAACTGTCGCGACCACCACCAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko00710 ko01100 ko01120 ko01200 map00630 map00710 map01100 map01120 map01200
Pfam Domains
Protein Families
RuBisCO_small

Protein Analysis

241

Amino Acids

27.39

Weight (kDa)

9.02

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_small PF00101 126 - 233 1.5e-38 Ribulose bisphosphate carboxylase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08000 FvH4_4g08000
malus_domestica MD04G1188500.v1.1 MD13G1252300.v1.1
prunus_persica Prupe.1G084500_v2.0.a1
pyrus_communis pycom13g22570
rosa_chinensis RchiOBHm_Chr4g0401741
rosa_laevigata RLG00000009138
rosa_multiflora Rmu_sc0001405.1_g000012 Rmu_sc0012591.1_g000003
rosa_roxburghii Rroxscaffold_5G00346210
rosa_rugosa Rorug04G0031400
rosa_samantha Rh4AG108600 Rh4BG102300 Rh4CG116800 Rh4DG100300
rosa_wichuraiana Rw4G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 58
AccII CGCG 1 cut(s) 707
AciI CCGC 1 cut(s) 56
AclWI GGATC 3 cut(s) 13, 451, 464
AcsI RAATTY 3 cut(s) 273, 374, 470
AfaI GTAC 3 cut(s) 521, 571, 620
AfiI CCNNNNNNNGG 3 cut(s) 563, 583, 664
AgsI TTSAA 6 cut(s) 86, 220, 270, 330, 417, 470
AjiI CACGTC 1 cut(s) 351
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 2 cut(s) 284, 553
AluI AGCT 2 cut(s) 284, 553
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 3 cut(s) 88, 322, 374
AlwI GGATC 3 cut(s) 13, 451, 464
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 3 cut(s) 209, 233, 339
ApoI RAATTY 3 cut(s) 273, 374, 470
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 69
AvaII GGWCC 1 cut(s) 303
BamHI GGATCC 1 cut(s) 456
BarI GAAGNNNNNNTAC 2 cut(s) 562, 594
BbsI GAAGAC 2 cut(s) 38, 353
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 196, 220, 326
BccI CCATC 3 cut(s) 294, 447, 521
BciT130I CCWGG 1 cut(s) 516
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 3 cut(s) 88, 322, 374
BisI GCNGC 3 cut(s) 210, 234, 340
BlsI GCNGC 3 cut(s) 211, 235, 341
Bme1390I CCNGG 1 cut(s) 516
Bme18I GGWCC 1 cut(s) 303
BmgBI CACGTC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 2 cut(s) 356, 458
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 3 cut(s) 83, 245, 501
BpiI GAAGAC 2 cut(s) 38, 353
BpuEI CTTGAG 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 515
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 3 cut(s) 563, 583, 664
Bse1I ACTGG 3 cut(s) 102, 542, 717
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 3 cut(s) 305, 458, 516
BseLI CCNNNNNNNGG 3 cut(s) 563, 583, 664
BseNI ACTGG 3 cut(s) 102, 542, 717
BseXI GCAGC 3 cut(s) 196, 220, 326
BseYI CCCAGC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 563, 583, 664
BsmAI GTCTC 3 cut(s) 88, 322, 374
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 4 cut(s) 5, 12, 360, 456
Bsp68I TCGCGA 1 cut(s) 707
BspACI CCGC 1 cut(s) 56
BspANI GGCC 1 cut(s) 680
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 2 cut(s) 356, 458
BspPI GGATC 3 cut(s) 13, 451, 464
BsrBI CCGCTC 1 cut(s) 58
BsrI ACTGG 3 cut(s) 102, 542, 717
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 4 cut(s) 5, 12, 360, 456
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 5 cut(s) 44, 71, 183, 316, 703
BstF5I GGATG 3 cut(s) 305, 458, 516
BstFNI CGCG 1 cut(s) 707
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 4 cut(s) 8, 15, 363, 459
BstMAI GTCTC 3 cut(s) 88, 322, 374
BstMBI GATC 4 cut(s) 5, 12, 360, 456
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 514
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 3 cut(s) 196, 220, 326
BstV2I GAAGAC 2 cut(s) 38, 353
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BsuRI GGCC 1 cut(s) 680
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 3 cut(s) 305, 458, 516
BtsIMutI CAGTG 4 cut(s) 40, 179, 677, 724
BtuMI TCGCGA 1 cut(s) 707
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 303
Csp6I GTAC 3 cut(s) 520, 570, 619
CviAII CATG 4 cut(s) 343, 439, 559, 676
CviJI RGCY 5 cut(s) 252, 284, 553, 680, 697
CviKI_1 RGCY 5 cut(s) 252, 284, 553, 680, 697
CviQI GTAC 3 cut(s) 520, 570, 619
DpnI GATC 4 cut(s) 7, 14, 362, 458
DpnII GATC 4 cut(s) 5, 12, 360, 456
Eco47I GGWCC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 4 cut(s) 346, 442, 562, 679
FatI CATG 4 cut(s) 342, 438, 558, 675
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 210, 234, 340
FokI GGATG 3 cut(s) 312, 465, 523
Fsp4HI GCNGC 3 cut(s) 210, 234, 340
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 210, 234, 340
GsaI CCCAGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 680
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 38
Hin1II CATG 4 cut(s) 346, 442, 562, 679
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HinfI GANTC 3 cut(s) 290, 382, 413
HpaII CCGG 1 cut(s) 227
HphI GGTGA 1 cut(s) 69
Hpy188I TCNGA 3 cut(s) 299, 409, 693
Hpy188III TCNNGA 4 cut(s) 27, 330, 445, 706
HpyAV CCTTC 3 cut(s) 276, 442, 588
HpyCH4III ACNGT 5 cut(s) 44, 71, 183, 316, 703
HpyCH4IV ACGT 2 cut(s) 350, 617
HpyCH4V TGCA 6 cut(s) 74, 236, 342, 422, 609, 675
HpySE526I ACGT 2 cut(s) 350, 617
Hsp92II CATG 4 cut(s) 346, 442, 562, 679
HspAI GCGC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 4 cut(s) 5, 12, 360, 456
Lsp1109I GCAGC 3 cut(s) 196, 220, 326
LweI GCATC 3 cut(s) 83, 245, 501
MaeII ACGT 2 cut(s) 350, 617
MalI GATC 4 cut(s) 7, 14, 362, 458
MbiI CCGCTC 1 cut(s) 58
MboI GATC 4 cut(s) 5, 12, 360, 456
MboII GAAGA 5 cut(s) 38, 208, 211, 358, 543
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 7 cut(s) 31, 120, 220, 273, 374, 417, 470
MlyI GAGTC 2 cut(s) 284, 376
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 2 cut(s) 404, 472
MseI TTAA 2 cut(s) 62, 192
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 516
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 516
MvnI CGCG 1 cut(s) 707
NdeII GATC 4 cut(s) 5, 12, 360, 456
NlaIII CATG 4 cut(s) 346, 442, 562, 679
NlaIV GGNNCC 2 cut(s) 356, 458
NruI TCGCGA 1 cut(s) 707
NspI RCATGY 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 385
PkrI GCNGC 3 cut(s) 211, 235, 341
PleI GAGTC 2 cut(s) 284, 376
PpsI GAGTC 2 cut(s) 284, 376
Psp6I CCWGG 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 2 cut(s) 356, 458
PspPI GGNCC 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 385
RruI TCGCGA 1 cut(s) 707
RsaI GTAC 3 cut(s) 521, 571, 620
RsaNI GTAC 3 cut(s) 520, 570, 619
SaqAI TTAA 2 cut(s) 62, 192
SatI GCNGC 3 cut(s) 210, 234, 340
Sau3AI GATC 4 cut(s) 5, 12, 360, 456
Sau96I GGNCC 1 cut(s) 303
SchI GAGTC 2 cut(s) 284, 376
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 1 cut(s) 591
SetI ASST 9 cut(s) 24, 141, 153, 286, 353, 396, 483, 555, 620
SfaNI GCATC 3 cut(s) 83, 245, 501
SinI GGWCC 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 7 cut(s) 31, 120, 220, 273, 374, 417, 470
SsiI CCGC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 514
TaaI ACNGT 5 cut(s) 44, 71, 183, 316, 703
TaiI ACGT 2 cut(s) 353, 620
TasI AATT 7 cut(s) 31, 120, 220, 273, 374, 417, 470
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 62, 192
Tru9I TTAA 2 cut(s) 62, 192
TscAI CASTG 4 cut(s) 47, 186, 677, 724
TseI GCWGC 3 cut(s) 209, 233, 339
TspDTI ATGAA 2 cut(s) 359, 609
TspRI CASTG 4 cut(s) 47, 186, 677, 724
Tth111I GACNNNGTC 1 cut(s) 385
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 3 cut(s) 273, 374, 470
XceI RCATGY 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.