Rw4G008830

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014712
Physical Location & Seq
Reverse (-)
19512439 .. 19513878
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008830.1

Sequence Viewer

Length: 384 bp
ATGAAGACGTGGAACCCGATCAACAACAAGAAATTTGAGACTCTGTCTTACCTCCCACCACTCTCTGATGATTCAATTGCAAAGGAGATTGACTACATGCTCAAGAAGGGATGGATCCCTTGCCTTGAATTTGATGAGGTGGGTTATGTTCATAGGGAGAATAGCAGGATGCCAGGGTACTATGATGGAAGATACTGGACATTGTGGAAGCTACCCATGTTTGGTTGTACCGACCCTTCTCTGGTGCTCAATGAAATCCAAGAGTGCAAAGAAACGTACCCAAATGCTTATATACGCTGTTTAGCATTTGACAACCAGAACCAGGGTCAGTGCATGTCCTTTATCATTCAGAAGCCAACCATCGCGGCCACCAACAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko00710 ko01100 ko01120 ko01200 map00630 map00710 map01100 map01120 map01200
Pfam Domains
Protein Families
RuBisCO_small

Protein Analysis

127

Amino Acids

14.8

Weight (kDa)

5.36

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_small PF00101 12 - 119 2.7e-39 Ribulose bisphosphate carboxylase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08000 FvH4_4g08000
malus_domestica MD04G1188500.v1.1 MD13G1252300.v1.1
prunus_persica Prupe.1G084500_v2.0.a1
pyrus_communis pycom13g22570
rosa_chinensis RchiOBHm_Chr4g0401741
rosa_laevigata RLG00000009138
rosa_multiflora Rmu_sc0001405.1_g000012 Rmu_sc0012591.1_g000003
rosa_roxburghii Rroxscaffold_5G00346210
rosa_rugosa Rorug04G0031400
rosa_samantha Rh4AG108600 Rh4BG102300 Rh4CG116800 Rh4DG100300
rosa_wichuraiana Rw4G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 365
AciI CCGC 1 cut(s) 365
AclWI GGATC 2 cut(s) 109, 122
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 2 cut(s) 32, 128
AfaI GTAC 3 cut(s) 179, 229, 278
AfiI CCNNNNNNNGG 3 cut(s) 221, 241, 322
AgsI TTSAA 2 cut(s) 75, 128
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 172, 321
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 109, 122
AoxI GGCC 1 cut(s) 366
ApoI RAATTY 2 cut(s) 32, 128
BamHI GGATCC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 220, 252
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 249
BccI CCATC 3 cut(s) 105, 179, 368
BciT130I CCWGG 2 cut(s) 174, 323
BcoDI GTCTC 1 cut(s) 32
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 174, 323
BmgBI CACGTC 1 cut(s) 9
BmiI GGNNCC 2 cut(s) 14, 116
BmrFI CCNGG 2 cut(s) 174, 323
BmsI GCATC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 173, 322
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 241, 322
Bse1I ACTGG 1 cut(s) 200
BseBI CCWGG 2 cut(s) 174, 323
BseDI CCNNGG 2 cut(s) 173, 322
BseGI GGATG 2 cut(s) 116, 174
BseLI CCNNNNNNNGG 3 cut(s) 221, 241, 322
BseNI ACTGG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 249
BslI CCNNNNNNNGG 3 cut(s) 221, 241, 322
BsmAI GTCTC 1 cut(s) 32
BsnI GGCC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 18, 114
BspACI CCGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 368
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 14, 116
BspPI GGATC 2 cut(s) 109, 122
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 173, 322
BssMI GATC 2 cut(s) 18, 114
Bst2UI CCWGG 2 cut(s) 174, 323
Bst4CI ACNGT 1 cut(s) 377
BstF5I GGATG 2 cut(s) 116, 174
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 2 cut(s) 21, 117
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 18, 114
BstNI CCWGG 2 cut(s) 174, 323
BstNSI RCATGY 2 cut(s) 100, 337
BstSCI CCNGG 2 cut(s) 172, 321
BstUI CGCG 1 cut(s) 365
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuRI GGCC 1 cut(s) 368
BtgZI GCGATG 1 cut(s) 346
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 2 cut(s) 116, 174
BtsIMutI CAGTG 2 cut(s) 335, 382
Csp6I GTAC 3 cut(s) 178, 228, 277
CviAII CATG 3 cut(s) 97, 217, 334
CviJI RGCY 3 cut(s) 211, 355, 368
CviKI_1 RGCY 3 cut(s) 211, 355, 368
CviQI GTAC 3 cut(s) 178, 228, 277
DpnI GATC 2 cut(s) 20, 116
DpnII GATC 2 cut(s) 18, 114
EaeI YGGCCR 1 cut(s) 366
EcoRII CCWGG 2 cut(s) 172, 321
FaeI CATG 3 cut(s) 100, 220, 337
FaiI YATR 8 cut(s) 98, 147, 153, 183, 218, 291, 293, 335
FatI CATG 3 cut(s) 96, 216, 333
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 2 cut(s) 123, 181
Fsp4HI GCNGC 1 cut(s) 366
GluI GCNGC 1 cut(s) 366
HaeIII GGCC 1 cut(s) 368
Hin1II CATG 3 cut(s) 100, 220, 337
HinfI GANTC 2 cut(s) 40, 71
Hpy188I TCNGA 2 cut(s) 67, 351
Hpy188III TCNNGA 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 100, 246
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 2 cut(s) 8, 275
HpyCH4V TGCA 3 cut(s) 80, 267, 333
HpySE526I ACGT 2 cut(s) 8, 275
Hsp92II CATG 3 cut(s) 100, 220, 337
Kzo9I GATC 2 cut(s) 18, 114
LpnPI CCDG 8 cut(s) 151, 159, 181, 186, 227, 308, 329, 335
LweI GCATC 1 cut(s) 159
MaeII ACGT 2 cut(s) 8, 275
MalI GATC 2 cut(s) 20, 116
MboI GATC 2 cut(s) 18, 114
MboII GAAGA 2 cut(s) 16, 201
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 32, 75, 128
MlyI GAGTC 1 cut(s) 34
MnlI CCTC 2 cut(s) 62, 130
MspR9I CCNGG 2 cut(s) 174, 323
MunI CAATTG 1 cut(s) 75
MvaI CCWGG 2 cut(s) 174, 323
MvnI CGCG 1 cut(s) 365
NdeII GATC 2 cut(s) 18, 114
NlaIII CATG 3 cut(s) 100, 220, 337
NlaIV GGNNCC 2 cut(s) 14, 116
NspI RCATGY 2 cut(s) 100, 337
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 71
PflFI GACNNNGTC 1 cut(s) 43
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 172, 321
PspGI CCWGG 2 cut(s) 172, 321
PspN4I GGNNCC 2 cut(s) 14, 116
PsuI RGATCY 1 cut(s) 114
PsyI GACNNNGTC 1 cut(s) 43
RsaI GTAC 3 cut(s) 179, 229, 278
RsaNI GTAC 3 cut(s) 178, 228, 277
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 2 cut(s) 18, 114
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 174, 323
SduI GDGCHC 1 cut(s) 249
SetI ASST 5 cut(s) 11, 54, 141, 213, 278
SfaNI GCATC 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 3 cut(s) 32, 75, 128
SsiI CCGC 1 cut(s) 365
StyD4I CCNGG 2 cut(s) 172, 321
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 2 cut(s) 11, 278
TasI AATT 3 cut(s) 32, 75, 128
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 1 cut(s) 71
TscAI CASTG 2 cut(s) 335, 382
TspDTI ATGAA 3 cut(s) 17, 140, 267
TspRI CASTG 2 cut(s) 335, 382
Tth111I GACNNNGTC 1 cut(s) 43
XapI RAATTY 2 cut(s) 32, 128
XceI RCATGY 2 cut(s) 100, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.