Rh4BG154600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017825
Physical Location & Seq
Forward (+)
27807751 .. 27847837
40087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG154600.1

Sequence Viewer

Length: 468 bp
ATGTTTGTTTCAGAGCTAGGGTTTATTGAATTTCAAAGAGAAAAATGGGAAGAGAGGCTCCATAGCAAACGTCGCAGCAGCAAGTGTCGTTCACGGTGGAGCAATTGGTCGCGGCCAATCACTAAAACCCTGATATCCTCCCCGATTTCGAAAGCTACGTCAACGAGAATTGAAGTGATATGGTTTTACTGCGATCTCATCTTGCATGAATGTACTCAGATTACCCTCGAAAGACTAGCAATGCCTCTGCTGTGCAATCTGTTGTGGCGGTTCAGCAGCATGAATGTATCAGAGTTGCCTTATGCATTTGCTCAGCACCCCGATACTCATTCAAGAGGTCGACTCGACTCACTCACTATCATTCAACAAGCAAATGGCACTAATACAATTTATGATCTGTTGAGCAATGAGGAGAGCCATGTCAAATGCATCCTAAGCCACACATCATGCTTTCCAGGAGGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

18.05

Weight (kDa)

8.6

Isoelectric Point (pI)

69.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 340
AccII CGCG 1 cut(s) 112
AciI CCGC 2 cut(s) 112, 268
AcoI YGGCCR 1 cut(s) 113
AcsI RAATTY 1 cut(s) 29
AfaI GTAC 1 cut(s) 214
AgsI TTSAA 5 cut(s) 29, 35, 173, 333, 365
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 2 cut(s) 16, 155
AluI AGCT 2 cut(s) 16, 155
AoxI GGCC 2 cut(s) 113, 460
ApeKI GCWGC 3 cut(s) 75, 78, 276
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AsuII TTCGAA 1 cut(s) 149
BbvI GCAGC 3 cut(s) 87, 90, 288
BciT130I CCWGG 1 cut(s) 456
BfaI CTAG 2 cut(s) 17, 236
BisI GCNGC 4 cut(s) 76, 79, 113, 277
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 4 cut(s) 77, 80, 114, 278
Bme1390I CCNGG 1 cut(s) 456
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 327, 359
Bpu10I CCTNAGC 1 cut(s) 434
Bpu1102I GCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 246, 412
BseBI CCWGG 1 cut(s) 456
BseGI GGATG 1 cut(s) 429
BseMI GCAATG 2 cut(s) 246, 412
BseMII CTCAG 2 cut(s) 230, 326
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 87, 90, 288
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 115, 462
BsnI GGCC 2 cut(s) 115, 462
Bsp119I TTCGAA 1 cut(s) 149
Bsp143I GATC 2 cut(s) 193, 394
Bsp1720I GCTNAGC 1 cut(s) 312
BspACI CCGC 2 cut(s) 112, 268
BspANI GGCC 2 cut(s) 115, 462
BspCNI CTCAG 2 cut(s) 229, 325
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 59
BspT104I TTCGAA 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 246, 412
BssMI GATC 2 cut(s) 193, 394
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 1 cut(s) 96
Bst6I CTCTTC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 149
BstDEI CTNAG 3 cut(s) 216, 312, 434
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 1 cut(s) 112
BstKTI GATC 2 cut(s) 196, 397
BstMBI GATC 2 cut(s) 193, 394
BstMWI GCNNNNNNNGC 2 cut(s) 72, 435
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 454
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 87, 90, 288
BsuRI GGCC 2 cut(s) 115, 462
BtsCI GGATG 1 cut(s) 429
Csp6I GTAC 1 cut(s) 213
CviAII CATG 4 cut(s) 206, 280, 419, 447
CviJI RGCY 7 cut(s) 16, 58, 115, 155, 417, 438, 462
CviKI_1 RGCY 7 cut(s) 16, 58, 115, 155, 417, 438, 462
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 3 cut(s) 216, 312, 434
DpnI GATC 2 cut(s) 195, 396
DpnII GATC 2 cut(s) 193, 394
EaeI YGGCCR 1 cut(s) 113
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 454
EcoRV GATATC 1 cut(s) 135
EcoT22I ATGCAT 2 cut(s) 307, 431
FaeI CATG 4 cut(s) 209, 283, 422, 450
FaiI YATR 8 cut(s) 63, 181, 207, 281, 303, 393, 420, 448
FatI CATG 4 cut(s) 205, 279, 418, 446
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 76, 79, 113, 277
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 4 cut(s) 76, 79, 113, 277
FspBI CTAG 2 cut(s) 17, 236
GluI GCNGC 4 cut(s) 76, 79, 113, 277
HaeIII GGCC 2 cut(s) 115, 462
Hin1II CATG 4 cut(s) 209, 283, 422, 450
HincII GTYRAC 2 cut(s) 162, 341
HindII GTYRAC 2 cut(s) 162, 341
HinfI GANTC 2 cut(s) 342, 347
Hpy166II GTNNAC 3 cut(s) 92, 162, 341
Hpy188I TCNGA 3 cut(s) 13, 219, 292
Hpy188III TCNNGA 1 cut(s) 333
Hpy8I GTNNAC 3 cut(s) 92, 162, 341
Hpy99I CGWCG 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4IV ACGT 2 cut(s) 70, 158
HpyCH4V TGCA 4 cut(s) 205, 255, 305, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 435
HpyF3I CTNAG 3 cut(s) 216, 312, 434
HpySE526I ACGT 2 cut(s) 70, 158
Hsp92II CATG 4 cut(s) 209, 283, 422, 450
Kzo9I GATC 2 cut(s) 193, 394
LmnI GCTCC 2 cut(s) 63, 99
LpnPI CCDG 2 cut(s) 143, 441
Lsp1109I GCAGC 3 cut(s) 87, 90, 288
LweI GCATC 1 cut(s) 438
MaeI CTAG 2 cut(s) 17, 236
MaeII ACGT 2 cut(s) 70, 158
MalI GATC 2 cut(s) 195, 396
MboI GATC 2 cut(s) 193, 394
MboII GAAGA 1 cut(s) 62
MfeI CAATTG 1 cut(s) 103
MluCI AATT 4 cut(s) 29, 103, 168, 387
MlyI GAGTC 2 cut(s) 336, 341
MnlI CCTC 7 cut(s) 48, 148, 236, 255, 329, 403, 452
Mph1103I ATGCAT 2 cut(s) 307, 431
MspR9I CCNGG 1 cut(s) 456
MunI CAATTG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 72, 435
NdeII GATC 2 cut(s) 193, 394
NlaIII CATG 4 cut(s) 209, 283, 422, 450
NlaIV GGNNCC 1 cut(s) 59
NsiI ATGCAT 2 cut(s) 307, 431
NspV TTCGAA 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 454
PkrI GCNGC 4 cut(s) 77, 80, 114, 278
PleI GAGTC 2 cut(s) 336, 341
PpsI GAGTC 2 cut(s) 336, 341
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 59
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SalI GTCGAC 1 cut(s) 339
SatI GCNGC 4 cut(s) 76, 79, 113, 277
Sau3AI GATC 2 cut(s) 193, 394
SchI GAGTC 2 cut(s) 336, 341
ScrFI CCNGG 1 cut(s) 456
SetI ASST 5 cut(s) 18, 73, 157, 161, 340
SfaNI GCATC 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 149
Sse9I AATT 4 cut(s) 29, 103, 168, 387
SsiI CCGC 2 cut(s) 112, 268
SspMI CTAG 2 cut(s) 17, 236
StyD4I CCNGG 1 cut(s) 454
TaaI ACNGT 1 cut(s) 96
TaiI ACGT 2 cut(s) 73, 161
TaqI TCGA 4 cut(s) 149, 228, 340, 345
TasI AATT 4 cut(s) 29, 103, 168, 387
TatI WGTACW 1 cut(s) 212
TauI GCSGC 1 cut(s) 115
TseI GCWGC 3 cut(s) 75, 78, 276
TspDTI ATGAA 2 cut(s) 222, 296
XapI RAATTY 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 2 cut(s) 17, 236
Zsp2I ATGCAT 2 cut(s) 307, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.