Rh4BG213200

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014907
Physical Location & Seq
Reverse (-)
37100550 .. 37104960
4411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG213200.1

Sequence Viewer

Length: 1092 bp
ATGCAGCCTGATCAAAGAAAAAAGTATACACCTGCAATAGATATCTGGAGCATTGGGTGCATCTTTGCAGAGCTTTTAACTGGAAAGCCTCTTTTTCCGGGCAAAAATGTTGTCCATCAGTTGGACCTCATGACTGATTTATTGGGAACACCATCTGCAGAAGCAATTGCTAGGGTACGCAATGAGAAGGCTCGCAGATACTTAAGCAGTATGAGAAAGAAGAGGCAGATTCCTTTTTCCCAGAAATTCCCAAATGCAGACCCACTTGCTCTACGTTTATTAGAAAGAATGTTAGCTTTTGAACCGAAGGATAGACCTACTGCTGAAGAGGCTCTTGCTGATCCCTATTTCAAGGGTTTGGCAAAAGTGGAGAGAGAACCTTCGGCTCAACCAGTTACCAAGATGGAATTTGAGTTTGAGAGGCGAAGGATAACCAAAGAAGATGTTAGAGAGCTTATTTATCGTGAGACTCTCGAGTATCATCCTAAGATGTTGAAGGAGTACTTAGACGGATCAGAGCCAACAGGATTCATGTATCCAAGTGCTGTTGACCATTTCAAGAAGCAATTTGCATATCTTGAGGAGCACTATGGAAATGGCACAGTTGGTGCTGCACCTGAGAGGCAACATGCATCACTACCCAGGGCATGTGTTTCGTATACCAATAACTCAGTGCAGCAACCAACAGAAGTCACAGAAGACCTTGCAAAATGCTGCATCAAAGAAATTGAGAAGCCAGTGGACAGGAACTGCGGAATCCCTACAACAAAGCCTCCAATTCAAGCTCCTCAGACCATCCAAGGAGCTGCAAGGCCTGGCAAAGTTGTTGGCTCAGTGTTACGTTTTAATAATTGCGGGGCAGCAGCTGCAGCAGAGACTCTTGAGCAGCGAAGGATGGTTAGGAATCCGGTTGTTCCAACTCAATATACGGCTGCCGCAGGCTCTTCATATCCTAGAAGAAATGCATCCTGCAAAAATGAGAGGGAAGACAATGAAGTAGAAGGTTCAAATGGGTTGCTGCCAAAGCCCCCTCAATACATTCCAAGGAAAGTTGCTGCTGCACAAGGTGGATCTGGAAGTCAATGGTATTGA

Protein Analysis

363

Amino Acids

40.74

Weight (kDa)

9.08

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 117 3.1e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014907)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19010
fragaria_vesca FvH4_4g14280
malus_domestica MD13G1181100.v1.1 MD16G1181800.v1.1
prunus_persica Prupe.1G149300_v2.0.a1 Prupe.1G149300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0418931
rosa_laevigata RLG00000007841
rosa_multiflora Rmu_sc0000935.1_g000048
rosa_roxburghii Rroxscaffold_5G00362010
rosa_rugosa Rorug04G0155100
rosa_samantha Rh4AG217000 Rh4BG213200 Rh4CG228800 Rh4DG215900
rosa_wichuraiana Rw4G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 121
AccI GTMKAC 2 cut(s) 26, 659
AciI CCGC 3 cut(s) 753, 855, 936
AclWI GGATC 3 cut(s) 335, 520, 1078
AcsI RAATTY 2 cut(s) 245, 407
AcuI CTGAAG 1 cut(s) 345
AdeI CACNNNGTG 1 cut(s) 1067
AfaI GTAC 2 cut(s) 177, 503
AfiI CCNNNNNNNGG 1 cut(s) 121
AflII CTTAAG 1 cut(s) 202
AgsI TTSAA 6 cut(s) 302, 352, 496, 559, 782, 1008
AjnI CCWGG 2 cut(s) 641, 814
AluBI AGCT 6 cut(s) 73, 296, 454, 785, 806, 866
AluI AGCT 6 cut(s) 73, 296, 454, 785, 806, 866
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 461, 869
AlwI GGATC 3 cut(s) 335, 520, 1078
AlwNI CAGNNNCTG 2 cut(s) 750, 866
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 1 cut(s) 812
ApoI RAATTY 2 cut(s) 245, 407
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspS9I GGNCC 1 cut(s) 124
AsuC2I CCSGG 1 cut(s) 99
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 124
BbsI GAAGAC 2 cut(s) 705, 993
Bbv12I GWGCWC 1 cut(s) 588
BccI CCATC 5 cut(s) 123, 160, 397, 803, 889
BceAI ACGGC 1 cut(s) 945
BcgI CGANNNNNNTGC 2 cut(s) 636, 670
BciT130I CCWGG 2 cut(s) 643, 816
BciVI GTATCC 1 cut(s) 546
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 2 cut(s) 461, 869
BfaI CTAG 2 cut(s) 171, 954
BfmI CTRYAG 2 cut(s) 156, 867
BfrI CTTAAG 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 40
BfuI GTATCC 1 cut(s) 546
BmcAI AGTACT 1 cut(s) 503
Bme1390I CCNGG 3 cut(s) 99, 643, 816
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 3 cut(s) 99, 643, 816
BmsI GCATC 4 cut(s) 69, 641, 726, 974
BpiI GAAGAC 2 cut(s) 705, 993
BpmI CTGGAG 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 599, 902
BpuMI CCSGG 1 cut(s) 99
BsaJI CCNNGG 4 cut(s) 641, 642, 799, 1043
BsaWI WCCGGW 1 cut(s) 907
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse1I ACTGG 3 cut(s) 85, 392, 737
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 2 cut(s) 643, 816
BseDI CCNNGG 4 cut(s) 641, 642, 799, 1043
BseGI GGATG 4 cut(s) 481, 795, 900, 965
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 4 cut(s) 609, 684, 803, 846
BseNI ACTGG 3 cut(s) 85, 392, 737
BseRI GAGGAG 2 cut(s) 596, 777
BsgI GTGCAG 3 cut(s) 597, 695, 1044
BshFI GGCC 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 2 cut(s) 98, 908
BslI CCNNNNNNNGG 1 cut(s) 121
BsmAI GTCTC 2 cut(s) 461, 869
BsnI GGCC 1 cut(s) 814
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 4 cut(s) 10, 340, 512, 1070
BspACI CCGC 3 cut(s) 753, 855, 936
BspANI GGCC 1 cut(s) 814
BspCNI CTCAG 4 cut(s) 610, 683, 802, 845
BspHI TCATGA 1 cut(s) 129
BspMAI CTGCAG 2 cut(s) 160, 871
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 335, 520, 1078
BspQI GCTCTTC 1 cut(s) 949
BspTI CTTAAG 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 187
BsrI ACTGG 3 cut(s) 85, 392, 737
BssECI CCNNGG 4 cut(s) 641, 642, 799, 1043
BssMI GATC 4 cut(s) 10, 340, 512, 1070
BssNAI GTATAC 2 cut(s) 27, 660
BssT1I CCWWGG 2 cut(s) 799, 1043
Bst1107I GTATAC 2 cut(s) 27, 660
Bst2UI CCWGG 2 cut(s) 643, 816
Bst4CI ACNGT 1 cut(s) 604
Bst6I CTCTTC 3 cut(s) 215, 321, 949
BstAFI CTTAAG 1 cut(s) 202
BstAPI GCANNNNNTGC 2 cut(s) 57, 866
BstC8I GCNNGC 2 cut(s) 193, 940
BstDEI CTNAG 6 cut(s) 486, 505, 618, 670, 789, 832
BstF5I GGATG 4 cut(s) 481, 795, 900, 965
BstKTI GATC 4 cut(s) 13, 343, 515, 1073
BstMAI GTCTC 2 cut(s) 461, 869
BstMBI GATC 4 cut(s) 10, 340, 512, 1070
BstMWI GCNNNNNNNGC 5 cut(s) 57, 329, 866, 869, 1024
BstNI CCWGG 2 cut(s) 643, 816
BstNSI RCATGY 2 cut(s) 632, 651
BstSCI CCNGG 3 cut(s) 97, 641, 814
BstSFI CTRYAG 2 cut(s) 156, 867
BstV2I GAAGAC 2 cut(s) 705, 993
BstX2I RGATCY 1 cut(s) 1070
BstYI RGATCY 1 cut(s) 1070
BstZ17I GTATAC 2 cut(s) 27, 660
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 1 cut(s) 814
BtsCI GGATG 4 cut(s) 481, 795, 900, 965
BtsIMutI CAGTG 3 cut(s) 678, 744, 840
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 193, 940
CaiI CAGNNNCTG 2 cut(s) 750, 866
CciI TCATGA 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 176, 502
CviAII CATG 4 cut(s) 130, 532, 629, 648
CviQI GTAC 2 cut(s) 176, 502
DdeI CTNAG 6 cut(s) 486, 505, 618, 670, 789, 832
DpnI GATC 4 cut(s) 12, 342, 514, 1072
DpnII GATC 4 cut(s) 10, 340, 512, 1070
DraIII CACNNNGTG 1 cut(s) 1067
Eam1104I CTCTTC 3 cut(s) 215, 321, 949
EarI CTCTTC 3 cut(s) 215, 321, 949
Eco130I CCWWGG 2 cut(s) 799, 1043
Eco147I AGGCCT 1 cut(s) 814
Eco32I GATATC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 641, 814
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 799, 1043
EcoT22I ATGCAT 2 cut(s) 634, 967
ErhI CCWWGG 2 cut(s) 799, 1043
FaeI CATG 4 cut(s) 133, 535, 632, 651
FalI AAGNNNNNCTT 4 cut(s) 318, 350, 488, 520
FatI CATG 4 cut(s) 129, 531, 628, 647
FauI CCCGC 1 cut(s) 848
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 2 cut(s) 26, 659
FokI GGATG 4 cut(s) 468, 782, 907, 952
FspBI CTAG 2 cut(s) 171, 954
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 1 cut(s) 814
HapII CCGG 2 cut(s) 98, 908
Hin1II CATG 4 cut(s) 133, 535, 632, 651
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 6 cut(s) 229, 469, 528, 756, 877, 904
HpaII CCGG 2 cut(s) 98, 908
Hpy166II GTNNAC 4 cut(s) 27, 550, 660, 742
Hpy188I TCNGA 2 cut(s) 517, 792
Hpy188III TCNNGA 8 cut(s) 46, 130, 464, 473, 559, 578, 881, 1074
Hpy8I GTNNAC 4 cut(s) 27, 550, 660, 742
HpyAV CCTTC 7 cut(s) 181, 301, 390, 420, 490, 885, 995
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4IV ACGT 2 cut(s) 274, 841
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 329, 866, 869, 1024
HpyF3I CTNAG 6 cut(s) 486, 505, 618, 670, 789, 832
HpySE526I ACGT 2 cut(s) 274, 841
Hsp92II CATG 4 cut(s) 133, 535, 632, 651
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 4 cut(s) 10, 340, 512, 1070
LguI GCTCTTC 1 cut(s) 949
LmnI GCTCC 4 cut(s) 48, 583, 790, 803
LweI GCATC 4 cut(s) 69, 641, 726, 974
MaeI CTAG 2 cut(s) 171, 954
MaeII ACGT 2 cut(s) 274, 841
MaeIII GTNAC 3 cut(s) 394, 691, 837
MalI GATC 4 cut(s) 12, 342, 514, 1072
MboI GATC 4 cut(s) 10, 340, 512, 1070
MboII GAAGA 7 cut(s) 232, 338, 452, 710, 936, 969, 998
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 1070
MhlI GDGCHC 1 cut(s) 588
MluCI AATT 7 cut(s) 165, 245, 407, 566, 726, 777, 850
MlyI GAGTC 2 cut(s) 463, 871
MmeI TCCRAC 2 cut(s) 102, 941
Mph1103I ATGCAT 2 cut(s) 634, 967
MseI TTAA 3 cut(s) 77, 203, 846
MspA1I CMGCKG 1 cut(s) 866
MspCI CTTAAG 1 cut(s) 202
MspI CCGG 2 cut(s) 98, 908
MspR9I CCNGG 3 cut(s) 99, 643, 816
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 2 cut(s) 643, 816
MwoI GCNNNNNNNGC 5 cut(s) 57, 329, 866, 869, 1024
NciI CCSGG 1 cut(s) 99
NdeII GATC 4 cut(s) 10, 340, 512, 1070
NlaIII CATG 4 cut(s) 133, 535, 632, 651
NmuCI GTSAC 1 cut(s) 691
NsiI ATGCAT 2 cut(s) 634, 967
NspI RCATGY 2 cut(s) 632, 651
PaeR7I CTCGAG 1 cut(s) 473
PagI TCATGA 1 cut(s) 129
PaqCI CACCTGC 1 cut(s) 40
PasI CCCWGGG 1 cut(s) 642
PceI AGGCCT 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 949
PfeI GAWTC 4 cut(s) 229, 528, 756, 904
PflMI CCANNNNNTGG 1 cut(s) 121
PleI GAGTC 2 cut(s) 463, 871
PpsI GAGTC 2 cut(s) 463, 871
Psp6I CCWGG 2 cut(s) 641, 814
PspGI CCWGG 2 cut(s) 641, 814
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 2 cut(s) 160, 871
PstNI CAGNNNCTG 2 cut(s) 750, 866
PsuI RGATCY 1 cut(s) 1070
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 2 cut(s) 177, 503
RsaNI GTAC 2 cut(s) 176, 502
SapI GCTCTTC 1 cut(s) 949
SaqAI TTAA 3 cut(s) 77, 203, 846
Sau3AI GATC 4 cut(s) 10, 340, 512, 1070
Sau96I GGNCC 1 cut(s) 124
ScaI AGTACT 1 cut(s) 503
SchI GAGTC 2 cut(s) 463, 871
ScrFI CCNGG 3 cut(s) 99, 643, 816
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 4 cut(s) 69, 641, 726, 974
SfcI CTRYAG 2 cut(s) 156, 867
Sfr274I CTCGAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 124
SlaI CTCGAG 1 cut(s) 473
SmlI CTYRAG 4 cut(s) 202, 473, 578, 881
SmoI CTYRAG 4 cut(s) 202, 473, 578, 881
Sse9I AATT 7 cut(s) 165, 245, 407, 566, 726, 777, 850
SseBI AGGCCT 1 cut(s) 814
SsiI CCGC 3 cut(s) 753, 855, 936
SspMI CTAG 2 cut(s) 171, 954
StuI AGGCCT 1 cut(s) 814
StyD4I CCNGG 3 cut(s) 97, 641, 814
StyI CCWWGG 2 cut(s) 799, 1043
TaaI ACNGT 1 cut(s) 604
TaiI ACGT 2 cut(s) 277, 844
TaqI TCGA 1 cut(s) 474
TasI AATT 7 cut(s) 165, 245, 407, 566, 726, 777, 850
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 4 cut(s) 229, 528, 756, 904
Tru1I TTAA 3 cut(s) 77, 203, 846
Tru9I TTAA 3 cut(s) 77, 203, 846
TscAI CASTG 3 cut(s) 678, 744, 840
TseFI GTSAC 1 cut(s) 691
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 3 cut(s) 520, 936, 1008
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 3 cut(s) 678, 744, 840
Van91I CCANNNNNTGG 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 2 cut(s) 245, 407
XceI RCATGY 2 cut(s) 632, 651
XhoI CTCGAG 1 cut(s) 473
XmiI GTMKAC 2 cut(s) 26, 659
XspI CTAG 2 cut(s) 171, 954
ZrmI AGTACT 1 cut(s) 503
Zsp2I ATGCAT 2 cut(s) 634, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.