Rh4BG323900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007590
Physical Location & Seq
Reverse (-)
48980832 .. 48985030
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG323900.1

Sequence Viewer

Length: 1245 bp
ATGGGAAGTAGAAGCTCCAAAGAGTCGAGTTCTAGGTTTTCTCATAGTGGTTCAACTGCTTCTTCTTCCTCATGGAATCAGTACCCGGAATCAGTACCATATGCTCAGCAAGGCCCGTATATTGCTACACCTCAGCATCACTATGCACCCTCCTCACAACTGCCAAGGAGGAGACTGGACAGGAAATACTCAAAGATATCTGACGATTATCGCTCCTTGGATGAGGTTACTAATGCTCTTGCACAAGCTGGCCTAGAGTCTTCTAATCTGATTGTTGGCATTGACTTCACAAAGAGCAATGAGTGGACAGGTTCGAGGTCATTTGGTCGGAAAAGCTTGCATCACATTGGAACTGGTCAAAATCCCTATGAACAAGCAATATCGATTATCGGGAAGACATTGTCCGCTTTTGATGAAGATAATCTAATTCCATGCTATGGGTTTGGAGATGCATCTACACATGATCAAGATGTCTTCAGCTTCTATCCAGATGACTGCGTTTGTACTGGATTCGAGGAAGTGCTGACACGATATAGAGAAATTGTTCCAACTCTTCGACTTGCAGGACCAACATCTTTTGCACCCATTATTGAGATGGCAATGACTATTGTTGAGCAAAGTGGAGGCCAATACCATGTTTTGCTGATCATTGCCGATGGTCAGGTGACAAGAAGTATTGATACACAACGTGGTCAACTAAGCCCACAAGAACAAAGGACTATTAATGCAATTGTAAGAGCAAGCAATTACCCCTTGTCTATAGTATTAGTCGGGGTTGGAGATGGGCCCTGGGACCTGATGAAGGAATTCGATGACAACATCCCTTCTCGGGCATTTGATAACTTGCAGTTTGTGAACTTTACCGAAATCATGTCAAAGAATGTGGATCTATCGAGAAAGCAGACAGAATTTGCTCTTTCAGCCTTGATGGAAATACCTTCCCAATATAAGGCCACCATAGCGCTAGACCTGTTGGGAGTACGAAATGGGAATTCCCCAGACAGAATTCCTCTTCCCCCACCTGCTGCAGTCTCTTCCATTAGAAACAACACTAGACCTTATACTGGATACGGCTCAAGCAGTTTTCAGCAGCGGACAGCTCCTCCTCATACTGGATATGATGCAGGAGTCAGCGCACCTCCTCATTCAACTTATGATAATCAGGTTTGCCCCATCTGTCTTACTAATCCGAAAGATATGGCCTTCGGTTGTGGGCATCAGGTAGACTATTTTCTCTATGCATAG

Protein Analysis

414

Amino Acids

45.64

Weight (kDa)

5.58

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 112 - 325 8.4e-72 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1030
Acc36I ACCTGC 1 cut(s) 1030
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 1 cut(s) 1224
AciI CCGC 2 cut(s) 405, 1093
AclWI GGATC 1 cut(s) 894
AcsI RAATTY 4 cut(s) 806, 908, 991, 1005
AcuI CTGAAG 1 cut(s) 460
AdeI CACNNNGTG 1 cut(s) 689
AfaI GTAC 4 cut(s) 83, 96, 505, 981
AfeI AGCGCT 1 cut(s) 963
AfiI CCNNNNNNNGG 6 cut(s) 437, 802, 829, 949, 1064, 1112
AgsI TTSAA 2 cut(s) 54, 1149
AjnI CCWGG 1 cut(s) 788
AluBI AGCT 5 cut(s) 15, 248, 336, 480, 1100
AluI AGCT 5 cut(s) 15, 248, 336, 480, 1100
Alw26I GTCTC 2 cut(s) 166, 1036
AlwI GGATC 1 cut(s) 894
Ama87I CYCGRG 1 cut(s) 828
Aor51HI AGCGCT 1 cut(s) 963
AoxI GGCC 6 cut(s) 112, 250, 625, 785, 951, 1200
ApaI GGGCCC 1 cut(s) 789
ApeKI GCWGC 2 cut(s) 1025, 1090
ApoI RAATTY 4 cut(s) 806, 908, 991, 1005
AseI ATTAAT 1 cut(s) 723
Asp700I GAANNNNTTC 2 cut(s) 543, 806
AspLEI GCGC 2 cut(s) 964, 1136
AspS9I GGNCC 5 cut(s) 113, 566, 785, 786, 793
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 676
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 2 cut(s) 566, 793
BaeGI GKGCMC 1 cut(s) 789
BanII GRGCYC 1 cut(s) 789
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbsI GAAGAC 3 cut(s) 252, 401, 466
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 1012, 1102
BccI CCATC 5 cut(s) 589, 650, 776, 922, 1181
BceAI ACGGC 1 cut(s) 1087
BciT130I CCWGG 1 cut(s) 790
BciVI GTATCC 1 cut(s) 1061
BclI TGATCA 2 cut(s) 463, 645
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 166, 1036
BfaI CTAG 4 cut(s) 33, 254, 965, 1053
BfmI CTRYAG 2 cut(s) 759, 1026
BfoI RGCGCY 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 1030
BfuI GTATCC 1 cut(s) 1061
BisI GCNGC 2 cut(s) 1026, 1091
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 2 cut(s) 1027, 1092
Bme1390I CCNGG 2 cut(s) 86, 790
Bme18I GGWCC 2 cut(s) 566, 793
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 5 cut(s) 113, 566, 785, 786, 793
BmiI GGNNCC 2 cut(s) 787, 794
BmrFI CCNGG 2 cut(s) 86, 790
BmsI GCATC 6 cut(s) 145, 349, 439, 461, 1111, 1225
BpiI GAAGAC 3 cut(s) 252, 401, 466
Bpu10I CCTNAGC 1 cut(s) 132
Bpu1102I GCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 1060
BpuMI CCSGG 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 164, 216, 788, 789
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 6 cut(s) 437, 802, 829, 949, 1064, 1112
Bse1I ACTGG 5 cut(s) 180, 358, 511, 1069, 1117
Bse3DI GCAATG 3 cut(s) 304, 606, 648
BseBI CCWGG 1 cut(s) 790
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 4 cut(s) 164, 216, 788, 789
BseGI GGATG 2 cut(s) 226, 819
BseLI CCNNNNNNNGG 6 cut(s) 437, 802, 829, 949, 1064, 1112
BseMI GCAATG 3 cut(s) 304, 606, 648
BseMII CTCAG 2 cut(s) 119, 146
BseNI ACTGG 5 cut(s) 180, 358, 511, 1069, 1117
BseRI GAGGAG 5 cut(s) 142, 184, 1092, 1095, 1131
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 2 cut(s) 1012, 1102
BshFI GGCC 6 cut(s) 114, 252, 627, 787, 953, 1202
BshVI ATCGAT 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 6 cut(s) 437, 802, 829, 949, 1064, 1112
BsmAI GTCTC 2 cut(s) 166, 1036
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 6 cut(s) 114, 252, 627, 787, 953, 1202
BsoBI CYCGRG 1 cut(s) 828
Bsp120I GGGCCC 1 cut(s) 785
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 3 cut(s) 463, 645, 886
Bsp1720I GCTNAGC 1 cut(s) 105
BspACI CCGC 2 cut(s) 405, 1093
BspANI GGCC 6 cut(s) 114, 252, 627, 787, 953, 1202
BspCNI CTCAG 2 cut(s) 118, 145
BspDI ATCGAT 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 787, 794
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 1 cut(s) 894
BsrDI GCAATG 3 cut(s) 304, 606, 648
BsrI ACTGG 5 cut(s) 180, 358, 511, 1069, 1117
BssECI CCNNGG 4 cut(s) 164, 216, 788, 789
BssMI GATC 3 cut(s) 463, 645, 886
BssT1I CCWWGG 2 cut(s) 164, 216
Bst2UI CCWGG 1 cut(s) 790
Bst6I CTCTTC 3 cut(s) 558, 1017, 1039
BstC8I GCNNGC 3 cut(s) 250, 338, 742
BstDEI CTNAG 3 cut(s) 105, 132, 698
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 2 cut(s) 226, 819
BstH2I RGCGCY 1 cut(s) 965
BstHHI GCGC 2 cut(s) 964, 1136
BstKTI GATC 3 cut(s) 466, 648, 889
BstMAI GTCTC 2 cut(s) 166, 1036
BstMBI GATC 3 cut(s) 463, 645, 886
BstMWI GCNNNNNNNGC 2 cut(s) 920, 959
BstNI CCWGG 1 cut(s) 790
BstSCI CCNGG 2 cut(s) 84, 788
BstSFI CTRYAG 2 cut(s) 759, 1026
BstSLI GKGCMC 1 cut(s) 789
BstV1I GCAGC 2 cut(s) 1012, 1102
BstV2I GAAGAC 3 cut(s) 252, 401, 466
BstX2I RGATCY 1 cut(s) 886
BstYI RGATCY 1 cut(s) 886
Bsu15I ATCGAT 1 cut(s) 383
BsuI GTATCC 1 cut(s) 1061
BsuRI GGCC 6 cut(s) 114, 252, 627, 787, 953, 1202
BsuTUI ATCGAT 1 cut(s) 383
BtsCI GGATG 2 cut(s) 226, 819
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 3 cut(s) 250, 338, 742
CfoI GCGC 2 cut(s) 964, 1136
Cfr13I GGNCC 5 cut(s) 113, 566, 785, 786, 793
ClaI ATCGAT 1 cut(s) 383
Csp6I GTAC 4 cut(s) 82, 95, 504, 980
CspCI CAANNNNNGTGG 2 cut(s) 864, 899
CviAII CATG 5 cut(s) 72, 432, 461, 635, 871
CviQI GTAC 4 cut(s) 82, 95, 504, 980
DdeI CTNAG 3 cut(s) 105, 132, 698
DpnI GATC 3 cut(s) 465, 647, 888
DpnII GATC 3 cut(s) 463, 645, 886
DraIII CACNNNGTG 1 cut(s) 689
Eam1104I CTCTTC 3 cut(s) 558, 1017, 1039
EarI CTCTTC 3 cut(s) 558, 1017, 1039
Eco130I CCWWGG 2 cut(s) 164, 216
Eco24I GRGCYC 1 cut(s) 789
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 2 cut(s) 566, 793
Eco47III AGCGCT 1 cut(s) 963
Eco57I CTGAAG 1 cut(s) 460
Eco88I CYCGRG 1 cut(s) 828
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoO109I RGGNCCY 2 cut(s) 786, 793
EcoRI GAATTC 3 cut(s) 806, 991, 1005
EcoRII CCWGG 1 cut(s) 788
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 164, 216
EcoT22I ATGCAT 2 cut(s) 454, 1243
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 2 cut(s) 164, 216
FaeI CATG 5 cut(s) 75, 435, 464, 638, 874
FaqI GGGAC 1 cut(s) 806
FatI CATG 5 cut(s) 71, 431, 460, 634, 870
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 2 cut(s) 463, 645
FblI GTMKAC 1 cut(s) 1224
Fnu4HI GCNGC 2 cut(s) 1026, 1091
FokI GGATG 2 cut(s) 233, 806
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 2 cut(s) 1026, 1091
FspBI CTAG 4 cut(s) 33, 254, 965, 1053
GlaI GCGC 2 cut(s) 963, 1135
GluI GCNGC 2 cut(s) 1026, 1091
HaeII RGCGCY 1 cut(s) 965
HaeIII GGCC 6 cut(s) 114, 252, 627, 787, 953, 1202
HapII CCGG 1 cut(s) 86
HhaI GCGC 2 cut(s) 964, 1136
Hin1II CATG 5 cut(s) 75, 435, 464, 638, 874
Hin6I GCGC 2 cut(s) 962, 1134
HinP1I GCGC 2 cut(s) 962, 1134
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 6 cut(s) 23, 76, 89, 257, 510, 1128
HpaII CCGG 1 cut(s) 86
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 4 cut(s) 306, 695, 856, 1225
Hpy188I TCNGA 4 cut(s) 202, 270, 330, 1191
Hpy188III TCNNGA 4 cut(s) 391, 467, 488, 894
Hpy8I GTNNAC 4 cut(s) 306, 695, 856, 1225
HpyAV CCTTC 4 cut(s) 796, 834, 948, 1213
HpyCH4IV ACGT 1 cut(s) 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 920, 959
HpyF3I CTNAG 3 cut(s) 105, 132, 698
HpySE526I ACGT 1 cut(s) 688
Hsp92II CATG 5 cut(s) 75, 435, 464, 638, 874
HspAI GCGC 2 cut(s) 962, 1134
Ksp22I TGATCA 2 cut(s) 463, 645
Kzo9I GATC 3 cut(s) 463, 645, 886
LmnI GCTCC 3 cut(s) 20, 218, 1105
Lsp1109I GCAGC 2 cut(s) 1012, 1102
LweI GCATC 6 cut(s) 145, 349, 439, 461, 1111, 1225
MaeI CTAG 4 cut(s) 33, 254, 965, 1053
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 2 cut(s) 226, 664
MalI GATC 3 cut(s) 465, 647, 888
MboI GATC 3 cut(s) 463, 645, 886
MboII GAAGA 9 cut(s) 54, 57, 252, 406, 428, 466, 545, 1004, 1026
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 8 cut(s) 426, 540, 729, 745, 806, 908, 991, 1005
MlyI GAGTC 3 cut(s) 32, 266, 1137
MmeI TCCRAC 3 cut(s) 308, 572, 757
Mph1103I ATGCAT 2 cut(s) 454, 1243
MroXI GAANNNNTTC 2 cut(s) 543, 806
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 1093
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 86, 790
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 1 cut(s) 790
MwoI GCNNNNNNNGC 2 cut(s) 920, 959
NciI CCSGG 1 cut(s) 86
NdeI CATATG 1 cut(s) 100
NdeII GATC 3 cut(s) 463, 645, 886
NlaIII CATG 5 cut(s) 75, 435, 464, 638, 874
NlaIV GGNNCC 2 cut(s) 787, 794
NmuCI GTSAC 1 cut(s) 664
NsiI ATGCAT 2 cut(s) 454, 1243
PaqCI CACCTGC 1 cut(s) 1030
PasI CCCWGGG 1 cut(s) 789
PdmI GAANNNNTTC 2 cut(s) 543, 806
PfeI GAWTC 3 cut(s) 76, 89, 510
PflFI GACNNNGTC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 2 cut(s) 1027, 1092
PleI GAGTC 3 cut(s) 31, 265, 1136
PpsI GAGTC 3 cut(s) 31, 265, 1136
PpuMI RGGWCCY 1 cut(s) 793
PshBI ATTAAT 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 793
Psp6I CCWGG 1 cut(s) 788
PspGI CCWGG 1 cut(s) 788
PspN4I GGNNCC 2 cut(s) 787, 794
PspOMI GGGCCC 1 cut(s) 785
PspPI GGNCC 5 cut(s) 113, 566, 785, 786, 793
PspPPI RGGWCCY 1 cut(s) 793
PstI CTGCAG 1 cut(s) 1030
PsuI RGATCY 1 cut(s) 886
PsyI GACNNNGTC 1 cut(s) 400
RsaI GTAC 4 cut(s) 83, 96, 505, 981
RsaNI GTAC 4 cut(s) 82, 95, 504, 980
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 2 cut(s) 1026, 1091
Sau3AI GATC 3 cut(s) 463, 645, 886
Sau96I GGNCC 5 cut(s) 113, 566, 785, 786, 793
SchI GAGTC 3 cut(s) 32, 266, 1137
ScrFI CCNGG 2 cut(s) 86, 790
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 6 cut(s) 145, 349, 439, 461, 1111, 1225
SfcI CTRYAG 2 cut(s) 759, 1026
SinI GGWCC 2 cut(s) 566, 793
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 1075
SmoI CTYRAG 1 cut(s) 1075
Sse9I AATT 8 cut(s) 426, 540, 729, 745, 806, 908, 991, 1005
SsiI CCGC 2 cut(s) 405, 1093
SspMI CTAG 4 cut(s) 33, 254, 965, 1053
StyD4I CCNGG 2 cut(s) 84, 788
StyI CCWWGG 2 cut(s) 164, 216
TaiI ACGT 1 cut(s) 691
TaqI TCGA 7 cut(s) 26, 314, 383, 513, 556, 810, 893
TasI AATT 8 cut(s) 426, 540, 729, 745, 806, 908, 991, 1005
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 3 cut(s) 76, 89, 510
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 2 cut(s) 1025, 1090
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 3 cut(s) 384, 429, 815
Tth111I GACNNNGTC 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 566, 793
VspI ATTAAT 1 cut(s) 723
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 4 cut(s) 806, 908, 991, 1005
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 1224
XmnI GAANNNNTTC 2 cut(s) 543, 806
XspI CTAG 4 cut(s) 33, 254, 965, 1053
Zsp2I ATGCAT 2 cut(s) 454, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.