Rh4BG373900

C2H2-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012139
Physical Location & Seq
Forward (+)
53023356 .. 53026187
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG373900.1

Sequence Viewer

Length: 1653 bp
ATGGAAGAGGATCAGAACCAGCACAAGAAATTCGTGTGCAAGTTTTGCAACAGGAGATTCCAATGTGGGAAGTCACTTGGTGGTCACATCAGGACCCATTTGAACAAGAAACCAACTCGGGAAAAAGAACAGGAGATGGAAGAAGACGAGTTTGCTAGAGTCACTAGGACTGCAAAGCTTTCGTCTTTGGATTCCGGGCCGGAAGGGCAATCCGGTTATCGTCTCAGGGAGAATCCCAAGAAGACTTGGAGGTTTGCCGATCACAAAGTCCCGAGTTTTCAATTCCAGCAGGAGAAAGTGTGCAAGGAATGTGGGAAAGGTTTTCCATCGCTGAAAGCTTTGTGTGGTCACATGGCTTGTCACTCGGACAAGGAGAGAAAGCTTGAAGAGATTGAAGAGTTTTCGGAGACTAGTGAGAAGCAGAAGCTGGTGATGGATAGTCAGTCTGATACTGAGACCTCATCAGCTCCAACAAAGCAGAGAAGGGTGCTGAAGAGAATGAGGGAGAACAATTTTGAAGTTCATGGTGGTTGTTCATCTGGTTCTGGAGTTGAGCAAGAACAGCAGGAAGTGGCTATTAGTTTGATGATGTTGTCTCGTGATTCGGGTTATAAAGGGGGTTTGAATTCGTTTGGTGAGTCTTCGGATAACAACTCGGTGATTCTAGAGGGCAAGTCATCTTCTATTGATATGAGAATTAGTACTAAGAGAGGTCTGAGTTATGCTGCTAAAGTGAATGATGTTATGGACTTGAAGAGAGCAAGAGAGAAAAAGTTGAAGTATGAGGAGGTTACTGTTTCTGACAATTCTGATTCTGGGTACTATATACATGAACCCAAGAAGGTCGAATCAGATGTTTCTGTAGATGGGTTTGTGAGGAATGGTGAGTTTAAGAAGTATAGAGCTGAATTCGGATCCGGGTTTGGGCGCTATGAGTCCTATGATGTTAAATCAAGAAGGGCGTTTAGCAGCAAGTCTGAAATGGTGAAGGAATTGCCACGAGAAGAAGCTTATGAACAAGTAGATGGGGGTTCTTTAAAGTATGCCTTGAGAAAATCCACCAAGAATGGCTTTCATAGACCATTTACTAGCGGCTCTTCCAGTGTAGAAGTTTACAAGAATGTTAGAAAGAGCAGCAAATATGAGTGCTTGACTTGTAACAAGACCTTCCATTCTCATAGAGCTCTTGGGGGTCACAGAGCAAGTCATACAAAGAGCAATGGCTACTCCGAGTCTATGTATGAAAGTGGAGAAAACAGCGTAGACAGTGATGAGTCTCCTATTCCAACACCTGAGAGTAAGGTTGTTGAGGACAATAAGCCTTACAAAGGCAAAACCCCAATTAACCAGGACTTGGGTGCAAAGAGAAATTCAGGGTCTAAGAGAAGCAAGGGGCATGAGTGCCCATTCTGCTTCAAGGTTTTCAAGTCAGGCCAAGCTTTAGGTGGTCACAAAAGGTCCCATTTTGTTGGAGGCTGTGAAGTCAAAACTGTGGTACTTGGACAAGAGCACCAGCATCCGGAAGAAGAAGAAGAAGAACATGAACTTGAAGTCCCTGAAAATGAGGCTGAAATCCCTACTCTATTTGATCTTAACCTTCCAGCTCCTATGGAGGAAGAAGCAAATGGGAATTTTGAGTACATGTCATGGTAG

Protein Analysis

550

Amino Acids

62.27

Weight (kDa)

7.03

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 100 - 123 1.7e-07 C2H2-type zinc finger
zf-C2H2_6 PF13912 381 - 405 3.9e-06 C2H2-type zinc finger
zf-C2H2_6 PF13912 466 - 488 3.7e-08 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26610
fragaria_vesca FvH4_4g30350
malus_domestica MD13G1064600.v1.1 MD16G1063100.v1.1
prunus_persica Prupe.1G283600_v2.0.a1
pyrus_communis pycom13g05700 pycom16g05530
rosa_chinensis RchiOBHm_Chr4g0438641
rosa_laevigata RLG00000006345
rosa_multiflora Rmu_co8449659.1_g000001 Rmu_sc0006646.1_g000008
rosa_roxburghii Rroxscaffold_5G00379630
rosa_rugosa Rorug04G0312100
rosa_samantha Rh4AG362500 Rh4BG373900 Rh4CG387800 Rh4DG368600
rosa_wichuraiana Rw0G007120 Rw0G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccB7I CCANNNNNTGG 1 cut(s) 1354
AccI GTMKAC 1 cut(s) 1263
AccIII TCCGGA 1 cut(s) 1519
AciI CCGC 1 cut(s) 1092
AclWI GGATC 3 cut(s) 18, 909, 922
AcsI RAATTY 5 cut(s) 29, 625, 908, 1369, 1630
AcuI CTGAAG 1 cut(s) 512
AdeI CACNNNGTG 1 cut(s) 80
AfaI GTAC 4 cut(s) 703, 821, 1497, 1640
AfiI CCNNNNNNNGG 4 cut(s) 924, 1328, 1354, 1519
AflIII ACRYGT 1 cut(s) 1641
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 1 cut(s) 1347
AloI GAACNNNNNNTCC 2 cut(s) 1536, 1568
Alw21I GWGCWC 2 cut(s) 1186, 1512
Alw26I GTCTC 5 cut(s) 227, 401, 449, 600, 1281
AlwI GGATC 3 cut(s) 18, 909, 922
AlwNI CAGNNNCTG 1 cut(s) 427
Ama87I CYCGRG 2 cut(s) 117, 271
Aor13HI TCCGGA 1 cut(s) 1519
AoxI GGCC 2 cut(s) 197, 1432
ApeKI GCWGC 3 cut(s) 725, 969, 1134
ApoI RAATTY 5 cut(s) 29, 625, 908, 1369, 1630
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 1 cut(s) 930
AspS9I GGNCC 3 cut(s) 93, 197, 1458
AsuC2I CCSGG 2 cut(s) 196, 919
AsuHPI GGTGA 5 cut(s) 442, 647, 670, 896, 997
AvaI CYCGRG 2 cut(s) 117, 271
AvaII GGWCC 2 cut(s) 93, 1458
BaeGI GKGCMC 1 cut(s) 1406
BamHI GGATCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 1186
BauI CACGAG 2 cut(s) 597, 999
BbsI GAAGAC 3 cut(s) 150, 248, 633
Bbv12I GWGCWC 2 cut(s) 1186, 1512
BbvI GCAGC 3 cut(s) 712, 981, 1146
BccI CCATC 5 cut(s) 130, 334, 427, 860, 1019
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 1 cut(s) 1349
BcnI CCSGG 2 cut(s) 196, 919
BcoDI GTCTC 5 cut(s) 227, 401, 449, 600, 1281
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 5 cut(s) 156, 165, 411, 665, 1089
BfmI CTRYAG 1 cut(s) 861
BfoI RGCGCY 1 cut(s) 931
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 4 cut(s) 726, 970, 1093, 1135
BlsI GCNGC 4 cut(s) 727, 971, 1094, 1136
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 3 cut(s) 196, 919, 1349
Bme18I GGWCC 2 cut(s) 93, 1458
BmeT110I CYCGRG 2 cut(s) 117, 271
BmgT120I GGNCC 3 cut(s) 93, 197, 1458
BmiI GGNNCC 3 cut(s) 95, 916, 1460
BmrFI CCNGG 3 cut(s) 196, 919, 1349
BmsI GCATC 1 cut(s) 1525
BpiI GAAGAC 3 cut(s) 150, 248, 633
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 1069
BpuMI CCSGG 2 cut(s) 196, 919
BsaI GGTCTC 1 cut(s) 449
BsaWI WCCGGW 2 cut(s) 212, 1519
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 4 cut(s) 924, 1328, 1354, 1519
Bse1I ACTGG 1 cut(s) 1101
Bse3DI GCAATG 1 cut(s) 1225
BseAI TCCGGA 1 cut(s) 1519
BseBI CCWGG 1 cut(s) 1349
BseGI GGATG 1 cut(s) 1516
BseLI CCNNNNNNNGG 4 cut(s) 924, 1328, 1354, 1519
BseMI GCAATG 1 cut(s) 1225
BseMII CTCAG 4 cut(s) 238, 444, 707, 1284
BseNI ACTGG 1 cut(s) 1101
BseRI GAGGAG 1 cut(s) 800
BseSI GKGCMC 1 cut(s) 1406
BseXI GCAGC 3 cut(s) 712, 981, 1146
BshFI GGCC 2 cut(s) 199, 1434
BsiHKAI GWGCWC 2 cut(s) 1186, 1512
BsiHKCI CYCGRG 2 cut(s) 117, 271
BsiSI CCGG 5 cut(s) 195, 200, 213, 918, 1520
BslFI GGGAC 3 cut(s) 254, 1444, 1538
BslI CCNNNNNNNGG 4 cut(s) 924, 1328, 1354, 1519
BsmAI GTCTC 5 cut(s) 227, 401, 449, 600, 1281
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 3 cut(s) 254, 1444, 1538
BsnI GGCC 2 cut(s) 199, 1434
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 2 cut(s) 117, 271
Bsp1286I GDGCHC 3 cut(s) 1186, 1406, 1512
Bsp13I TCCGGA 1 cut(s) 1519
Bsp143I GATC 4 cut(s) 10, 259, 914, 1588
BspACI CCGC 1 cut(s) 1092
BspANI GGCC 2 cut(s) 199, 1434
BspCNI CTCAG 4 cut(s) 237, 445, 708, 1285
BspEI TCCGGA 1 cut(s) 1519
BspLI GGNNCC 3 cut(s) 95, 916, 1460
BspPI GGATC 3 cut(s) 18, 909, 922
BspQI GCTCTTC 1 cut(s) 1102
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 1225
BsrI ACTGG 1 cut(s) 1101
BssMI GATC 4 cut(s) 10, 259, 914, 1588
BssSI CACGAG 2 cut(s) 597, 999
Bst2BI CACGAG 2 cut(s) 597, 999
Bst2UI CCWGG 1 cut(s) 1349
Bst4CI ACNGT 3 cut(s) 796, 1268, 1492
Bst6I CTCTTC 5 cut(s) 381, 390, 488, 749, 1102
BstAPI GCANNNNNTGC 1 cut(s) 45
BstDEI CTNAG 6 cut(s) 224, 453, 705, 716, 1293, 1380
BstENI CCTNNNNNAGG 1 cut(s) 1326
BstF5I GGATG 1 cut(s) 1516
BstH2I RGCGCY 1 cut(s) 931
BstHHI GCGC 1 cut(s) 930
BstKTI GATC 4 cut(s) 13, 262, 917, 1591
BstMAI GTCTC 5 cut(s) 227, 401, 449, 600, 1281
BstMBI GATC 4 cut(s) 10, 259, 914, 1588
BstMWI GCNNNNNNNGC 4 cut(s) 45, 205, 562, 1410
BstNI CCWGG 1 cut(s) 1349
BstNSI RCATGY 1 cut(s) 1645
BstSCI CCNGG 3 cut(s) 194, 917, 1347
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 1406
BstV1I GCAGC 3 cut(s) 712, 981, 1146
BstV2I GAAGAC 3 cut(s) 150, 248, 633
BstX2I RGATCY 1 cut(s) 914
BstXI CCANNNNNNTGG 1 cut(s) 1469
BstYI RGATCY 1 cut(s) 914
BsuRI GGCC 2 cut(s) 199, 1434
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 1516
BtsIMutI CAGTG 2 cut(s) 1108, 1273
CaiI CAGNNNCTG 1 cut(s) 427
CfoI GCGC 1 cut(s) 930
Cfr13I GGNCC 3 cut(s) 93, 197, 1458
Csp6I GTAC 4 cut(s) 702, 820, 1496, 1639
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 7 cut(s) 352, 524, 830, 1397, 1541, 1642, 1647
CviQI GTAC 4 cut(s) 702, 820, 1496, 1639
DdeI CTNAG 6 cut(s) 224, 453, 705, 716, 1293, 1380
DpnI GATC 4 cut(s) 12, 261, 916, 1590
DpnII GATC 4 cut(s) 10, 259, 914, 1588
DraI TTTAAA 1 cut(s) 1038
DraIII CACNNNGTG 1 cut(s) 80
Eam1104I CTCTTC 5 cut(s) 381, 390, 488, 749, 1102
EarI CTCTTC 5 cut(s) 381, 390, 488, 749, 1102
Ecl136II GAGCTC 1 cut(s) 1184
Eco24I GRGCYC 1 cut(s) 1186
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 93, 1458
Eco53kI GAGCTC 1 cut(s) 1184
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 2 cut(s) 117, 271
EcoICRI GAGCTC 1 cut(s) 1184
EcoNI CCTNNNNNAGG 1 cut(s) 1326
EcoO109I RGGNCCY 2 cut(s) 93, 1458
EcoRI GAATTC 2 cut(s) 625, 908
EcoRII CCWGG 1 cut(s) 1347
EcoT38I GRGCYC 1 cut(s) 1186
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 7 cut(s) 355, 527, 833, 1400, 1544, 1645, 1650
FalI AAGNNNNNCTT 4 cut(s) 1031, 1063, 1055, 1087
FaqI GGGAC 3 cut(s) 254, 1444, 1538
FatI CATG 7 cut(s) 351, 523, 829, 1396, 1540, 1641, 1646
FblI GTMKAC 1 cut(s) 1263
Fnu4HI GCNGC 4 cut(s) 726, 970, 1093, 1135
FokI GGATG 1 cut(s) 1503
FriOI GRGCYC 1 cut(s) 1186
Fsp4HI GCNGC 4 cut(s) 726, 970, 1093, 1135
FspBI CTAG 5 cut(s) 156, 165, 411, 665, 1089
GlaI GCGC 1 cut(s) 929
GluI GCNGC 4 cut(s) 726, 970, 1093, 1135
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 931
HaeIII GGCC 2 cut(s) 199, 1434
HapII CCGG 5 cut(s) 195, 200, 213, 918, 1520
HhaI GCGC 1 cut(s) 930
Hin1II CATG 7 cut(s) 355, 527, 833, 1400, 1544, 1645, 1650
Hin6I GCGC 1 cut(s) 928
HinP1I GCGC 1 cut(s) 928
HindIII AAGCTT 5 cut(s) 176, 336, 380, 1008, 1437
HpaII CCGG 5 cut(s) 195, 200, 213, 918, 1520
HphI GGTGA 5 cut(s) 442, 647, 670, 896, 997
Hpy166II GTNNAC 2 cut(s) 1114, 1264
Hpy188III TCNNGA 8 cut(s) 91, 119, 271, 546, 599, 665, 954, 1520
Hpy8I GTNNAC 2 cut(s) 1114, 1264
HpyAV CCTTC 7 cut(s) 197, 477, 835, 951, 982, 1177, 1607
HpyCH4III ACNGT 3 cut(s) 796, 1268, 1492
HpyCH4V TGCA 5 cut(s) 39, 48, 173, 303, 1361
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 205, 562, 1410
HpyF3I CTNAG 6 cut(s) 224, 453, 705, 716, 1293, 1380
Hsp92II CATG 7 cut(s) 355, 527, 833, 1400, 1544, 1645, 1650
HspAI GCGC 1 cut(s) 928
Kpn2I TCCGGA 1 cut(s) 1519
Kzo9I GATC 4 cut(s) 10, 259, 914, 1588
LguI GCTCTTC 1 cut(s) 1102
LmnI GCTCC 2 cut(s) 472, 1609
Lsp1109I GCAGC 3 cut(s) 712, 981, 1146
LweI GCATC 1 cut(s) 1525
MaeI CTAG 5 cut(s) 156, 165, 411, 665, 1089
MaeIII GTNAC 9 cut(s) 72, 83, 160, 347, 359, 790, 1157, 1193, 1448
MalI GATC 4 cut(s) 12, 261, 916, 1590
MboI GATC 4 cut(s) 10, 259, 914, 1588
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 3 cut(s) 1186, 1406, 1512
MlyI GAGTC 5 cut(s) 168, 647, 944, 1241, 1283
MmeI TCCRAC 3 cut(s) 494, 1310, 1450
MroI TCCGGA 1 cut(s) 1519
MseI TTAA 5 cut(s) 891, 948, 1037, 1344, 1593
MspI CCGG 5 cut(s) 195, 200, 213, 918, 1520
MspR9I CCNGG 3 cut(s) 196, 919, 1349
MvaI CCWGG 1 cut(s) 1349
MwoI GCNNNNNNNGC 4 cut(s) 45, 205, 562, 1410
NciI CCSGG 2 cut(s) 196, 919
NdeII GATC 4 cut(s) 10, 259, 914, 1588
NlaIII CATG 7 cut(s) 355, 527, 833, 1400, 1544, 1645, 1650
NlaIV GGNNCC 3 cut(s) 95, 916, 1460
NmuCI GTSAC 7 cut(s) 72, 83, 160, 347, 359, 1193, 1448
NspI RCATGY 1 cut(s) 1645
PciI ACATGT 1 cut(s) 1641
PciSI GCTCTTC 1 cut(s) 1102
PfeI GAWTC 7 cut(s) 57, 191, 232, 602, 661, 812, 848
PflMI CCANNNNNTGG 1 cut(s) 1354
PkrI GCNGC 4 cut(s) 727, 971, 1094, 1136
PleI GAGTC 5 cut(s) 167, 646, 943, 1240, 1282
PpsI GAGTC 5 cut(s) 167, 646, 943, 1240, 1282
PpuMI RGGWCCY 2 cut(s) 93, 1458
PscI ACATGT 1 cut(s) 1641
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 1186
Psp5II RGGWCCY 2 cut(s) 93, 1458
Psp6I CCWGG 1 cut(s) 1347
PspGI CCWGG 1 cut(s) 1347
PspN4I GGNNCC 3 cut(s) 95, 916, 1460
PspPI GGNCC 3 cut(s) 93, 197, 1458
PspPPI RGGWCCY 2 cut(s) 93, 1458
PstNI CAGNNNCTG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 4 cut(s) 703, 821, 1497, 1640
RsaNI GTAC 4 cut(s) 702, 820, 1496, 1639
SacI GAGCTC 1 cut(s) 1186
SapI GCTCTTC 1 cut(s) 1102
SaqAI TTAA 5 cut(s) 891, 948, 1037, 1344, 1593
SatI GCNGC 4 cut(s) 726, 970, 1093, 1135
Sau3AI GATC 4 cut(s) 10, 259, 914, 1588
Sau96I GGNCC 3 cut(s) 93, 197, 1458
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 5 cut(s) 168, 647, 944, 1241, 1283
ScrFI CCNGG 3 cut(s) 196, 919, 1349
SduI GDGCHC 3 cut(s) 1186, 1406, 1512
SfaNI GCATC 1 cut(s) 1525
SfcI CTRYAG 1 cut(s) 861
SinI GGWCC 2 cut(s) 93, 1458
SmlI CTYRAG 1 cut(s) 1048
SmoI CTYRAG 1 cut(s) 1048
SpeI ACTAGT 1 cut(s) 410
SsiI CCGC 1 cut(s) 1092
SspMI CTAG 5 cut(s) 156, 165, 411, 665, 1089
SstI GAGCTC 1 cut(s) 1186
StyD4I CCNGG 3 cut(s) 194, 917, 1347
TaaI ACNGT 3 cut(s) 796, 1268, 1492
TaqI TCGA 1 cut(s) 846
TatI WGTACW 2 cut(s) 701, 1638
TauI GCSGC 1 cut(s) 1095
TfiI GAWTC 7 cut(s) 57, 191, 232, 602, 661, 812, 848
Tru1I TTAA 5 cut(s) 891, 948, 1037, 1344, 1593
Tru9I TTAA 5 cut(s) 891, 948, 1037, 1344, 1593
TscAI CASTG 2 cut(s) 1108, 1273
TseFI GTSAC 7 cut(s) 72, 83, 160, 347, 359, 1193, 1448
TseI GCWGC 3 cut(s) 725, 969, 1134
Tsp45I GTSAC 7 cut(s) 72, 83, 160, 347, 359, 1193, 1448
TspDTI ATGAA 7 cut(s) 512, 525, 846, 1029, 1064, 1257, 1557
TspRI CASTG 2 cut(s) 1108, 1273
Van91I CCANNNNNTGG 1 cut(s) 1354
VpaK11BI GGWCC 2 cut(s) 93, 1458
XagI CCTNNNNNAGG 1 cut(s) 1326
XapI RAATTY 5 cut(s) 29, 625, 908, 1369, 1630
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 1645
XcmI CCANNNNNNNNNTGG 1 cut(s) 1442
XmiI GTMKAC 1 cut(s) 1263
XspI CTAG 5 cut(s) 156, 165, 411, 665, 1089
ZrmI AGTACT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.