Rh4CG404500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012745
Physical Location & Seq
Reverse (-)
65212970 .. 65215760
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG404500.1

Sequence Viewer

Length: 459 bp
ATGTCGACTCCTGCAAGGAAGAGGCTGATGAGGGATTTCAAGAGGTTGCAGCAGGACCCTCCTGCTGGAATAAGCGGTGCTCCTCAAGACAATAATATAATGCTTTGGAATGCTGTTATATTCGGGCCCGATGACACCCCGTGGGATGGAGGTACGTTTAAGTTGACACTTCAATTTTCCGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCTCGAATGTTTCATCCAAACATTTATGCGGATGGAAGCATTTGTTTGGACATCCTACAGAATCAGTGGAGCCCTATATATGATGTAGCAGCTATACTTACTTCCATTCAGTCATTGCTGTGTGATCCAAACCCGAACTCTCCAGCAAATTCAGAAGCTGCACGTATGTTCAGCGAGAACAAGCGTGAATACAACAGAAGAATCCGTGAGATTGTTGAGCAGAGTTGGACTGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

152

Amino Acids

17.35

Weight (kDa)

5.4

Isoelectric Point (pI)

73.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 144 1.1e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0012745)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14400 AT1G14400 AT2G02760 AT2G02760 AT2G02760 AT2G02760
fragaria_vesca FvH4_4g31740
rosa_chinensis RchiOBHm_Chr4g0440341
rosa_laevigata RLG00000006190
rosa_multiflora Rmu_sc0001685.1_g000043 Rmu_sc0033827.1_g000001
rosa_roxburghii Rroxscaffold_5G00381220
rosa_rugosa Rorug04G0325700
rosa_samantha Rh4AG376700 Rh4BG389700 Rh4CG404500 Rh4DG383400
rosa_wichuraiana Rw4G032250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 75, 248
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 367
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 2 cut(s) 65, 146
AgsI TTSAA 2 cut(s) 40, 173
AluBI AGCT 2 cut(s) 311, 377
AluI AGCT 2 cut(s) 311, 377
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 1 cut(s) 125
ApaI GGGCCC 1 cut(s) 129
ApeKI GCWGC 3 cut(s) 49, 308, 377
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 3 cut(s) 55, 125, 126
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 129, 293
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 3 cut(s) 61, 320, 364
BccI CCATC 2 cut(s) 140, 245
BfmI CTRYAG 1 cut(s) 275
BisI GCNGC 3 cut(s) 50, 309, 378
BlsI GCNGC 3 cut(s) 51, 310, 379
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 55, 125, 126
BmiI GGNNCC 3 cut(s) 57, 127, 290
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 140, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 146
Bse3DI GCAATG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 140, 180
BseGI GGATG 4 cut(s) 151, 232, 256, 270
BseLI CCNNNNNNNGG 2 cut(s) 65, 146
BseMI GCAATG 1 cut(s) 332
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 3 cut(s) 61, 320, 364
BsgI GTGCAG 1 cut(s) 363
BshFI GGCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 65, 146
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 127
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 82, 129, 293
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 2 cut(s) 75, 248
BspANI GGCC 1 cut(s) 127
BspLI GGNNCC 3 cut(s) 57, 127, 290
BspPI GGATC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 140, 180
BssMI GATC 1 cut(s) 343
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 383
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 4 cut(s) 151, 232, 256, 270
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 1 cut(s) 346
BstMBI GATC 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 275
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 61, 320, 364
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 140
BtsCI GGATG 4 cut(s) 151, 232, 256, 270
BtsIMutI CAGTG 2 cut(s) 213, 290
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 3 cut(s) 55, 125, 126
Csp6I GTAC 1 cut(s) 153
CviJI RGCY 6 cut(s) 25, 127, 199, 291, 311, 377
CviKI_1 RGCY 6 cut(s) 25, 127, 199, 291, 311, 377
CviQI GTAC 1 cut(s) 153
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
DraIII CACNNNGTG 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco24I GRGCYC 2 cut(s) 129, 293
Eco47I GGWCC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 129, 293
FaiI YATR 8 cut(s) 98, 119, 246, 296, 298, 300, 314, 386
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 50, 309, 378
FokI GGATG 4 cut(s) 158, 219, 257, 263
FriOI GRGCYC 2 cut(s) 129, 293
Fsp4HI GCNGC 3 cut(s) 50, 309, 378
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 50, 309, 378
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 1 cut(s) 127
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HincII GTYRAC 2 cut(s) 6, 165
HindII GTYRAC 2 cut(s) 6, 165
HinfI GANTC 3 cut(s) 7, 280, 420
Hpy166II GTNNAC 2 cut(s) 6, 165
Hpy188I TCNGA 2 cut(s) 181, 373
Hpy188III TCNNGA 3 cut(s) 40, 86, 222
Hpy8I GTNNAC 2 cut(s) 6, 165
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 2 cut(s) 155, 382
HpyCH4V TGCA 3 cut(s) 14, 49, 380
HpySE526I ACGT 2 cut(s) 155, 382
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 1 cut(s) 343
LmnI GCTCC 2 cut(s) 85, 288
LpnPI CCDG 5 cut(s) 24, 38, 51, 75, 375
Lsp1109I GCAGC 3 cut(s) 61, 320, 364
MaeII ACGT 2 cut(s) 155, 382
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 2 cut(s) 31, 429
MhlI GDGCHC 3 cut(s) 82, 129, 293
MluCI AATT 2 cut(s) 173, 367
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 7 cut(s) 15, 24, 36, 69, 93, 143, 175
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 115
NdeII GATC 1 cut(s) 343
NlaIV GGNNCC 3 cut(s) 57, 127, 290
PctI GAATGC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 280, 420
PkrI GCNGC 3 cut(s) 51, 310, 379
Ppu21I YACGTR 1 cut(s) 383
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 3 cut(s) 57, 127, 290
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 3 cut(s) 55, 125, 126
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 337
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 3 cut(s) 50, 309, 378
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 3 cut(s) 55, 125, 126
SduI GDGCHC 3 cut(s) 82, 129, 293
SetI ASST 6 cut(s) 47, 154, 158, 313, 379, 385
SfcI CTRYAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 2 cut(s) 173, 367
SsiI CCGC 2 cut(s) 75, 248
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 2 cut(s) 158, 385
TaqI TCGA 2 cut(s) 5, 223
TasI AATT 2 cut(s) 173, 367
TfiI GAWTC 2 cut(s) 280, 420
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 213, 290
TseI GCWGC 3 cut(s) 49, 308, 377
TspDTI ATGAA 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 213, 290
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.