Rh4DG383400

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012745
Physical Location & Seq
Reverse (-)
60073550 .. 60076336
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG383400.1

Sequence Viewer

Length: 360 bp
ATGTCGACTCCTGCAAGGAAGAGACTGATGAGGGATTTCAAGAGGTTGCAGCAGGACCCTCCTGCTGGAATAAGCGGTGCTCCTCAAGACAATAATATAATGCTTTGGAATGCTGTTATATTTGGGCCCGATGACACCCCATGGGATGGAGGTACGTTCAAGTTGACACTTCAATTTTCCGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCTCGAATGTTTCATCCAAACATTTATGCGGATGGAAGCATTTGTTTGGACATCCTACAGAATCAGTGGAGCCCTATATATGATGTAGCAGCTATACTTACTTCCATTCAGGTATTCGATGTTGTTTCCATTGCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

119

Amino Acids

13.49

Weight (kDa)

5.09

Isoelectric Point (pI)

63.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 111 8.5e-41 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012745)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14400 AT1G14400 AT2G02760 AT2G02760 AT2G02760 AT2G02760
fragaria_vesca FvH4_4g31740
rosa_chinensis RchiOBHm_Chr4g0440341
rosa_laevigata RLG00000006190
rosa_multiflora Rmu_sc0001685.1_g000043 Rmu_sc0033827.1_g000001
rosa_roxburghii Rroxscaffold_5G00381220
rosa_rugosa Rorug04G0325700
rosa_samantha Rh4AG376700 Rh4BG389700 Rh4CG404500 Rh4DG383400
rosa_wichuraiana Rw4G032250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 146, 354
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 75, 248
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 3 cut(s) 65, 146, 354
AgsI TTSAA 3 cut(s) 40, 160, 173
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 16
AoxI GGCC 1 cut(s) 125
ApaI GGGCCC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 49, 308
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 3 cut(s) 55, 125, 126
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 129, 293
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 61, 320
BccI CCATC 2 cut(s) 140, 245
BcoDI GTCTC 1 cut(s) 16
BfmI CTRYAG 1 cut(s) 275
BisI GCNGC 2 cut(s) 50, 309
BlsI GCNGC 2 cut(s) 51, 310
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 55, 125, 126
BmiI GGNNCC 3 cut(s) 57, 127, 290
BpuEI CTTGAG 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 140, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 146, 354
Bse3DI GCAATG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 140, 180
BseGI GGATG 4 cut(s) 151, 232, 256, 270
BseLI CCNNNNNNNGG 3 cut(s) 65, 146, 354
BseMI GCAATG 1 cut(s) 348
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 2 cut(s) 61, 320
BshFI GGCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 65, 146, 354
BsmAI GTCTC 1 cut(s) 16
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 127
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 82, 129, 293
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 2 cut(s) 75, 248
BspANI GGCC 1 cut(s) 127
BspLI GGNNCC 3 cut(s) 57, 127, 290
BsrDI GCAATG 1 cut(s) 348
BssECI CCNNGG 2 cut(s) 140, 180
BssT1I CCWWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 14
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 4 cut(s) 151, 232, 256, 270
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 275
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 61, 320
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 140
BtsCI GGATG 4 cut(s) 151, 232, 256, 270
BtsIMutI CAGTG 2 cut(s) 213, 290
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 3 cut(s) 55, 125, 126
Csp6I GTAC 1 cut(s) 153
CviAII CATG 1 cut(s) 141
CviJI RGCY 4 cut(s) 127, 199, 291, 311
CviKI_1 RGCY 4 cut(s) 127, 199, 291, 311
CviQI GTAC 1 cut(s) 153
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 129, 293
Eco47I GGWCC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 2 cut(s) 129, 293
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 1 cut(s) 144
FaiI YATR 8 cut(s) 98, 119, 142, 246, 296, 298, 300, 314
FatI CATG 1 cut(s) 140
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 50, 309
FokI GGATG 4 cut(s) 158, 219, 257, 263
FriOI GRGCYC 2 cut(s) 129, 293
Fsp4HI GCNGC 2 cut(s) 50, 309
GlaI GCGC 1 cut(s) 212
GluI GCNGC 2 cut(s) 50, 309
HaeIII GGCC 1 cut(s) 127
HhaI GCGC 1 cut(s) 213
Hin1II CATG 1 cut(s) 144
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HincII GTYRAC 2 cut(s) 6, 165
HindII GTYRAC 2 cut(s) 6, 165
HinfI GANTC 2 cut(s) 7, 280
Hpy166II GTNNAC 2 cut(s) 6, 165
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 3 cut(s) 40, 86, 222
Hpy8I GTNNAC 2 cut(s) 6, 165
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 2 cut(s) 14, 49
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 1 cut(s) 144
HspAI GCGC 1 cut(s) 211
LmnI GCTCC 2 cut(s) 85, 288
LpnPI CCDG 5 cut(s) 24, 38, 51, 75, 314
Lsp1109I GCAGC 2 cut(s) 61, 320
MaeII ACGT 1 cut(s) 155
MboII GAAGA 1 cut(s) 31
MhlI GDGCHC 3 cut(s) 82, 129, 293
MluCI AATT 1 cut(s) 173
MnlI CCTC 6 cut(s) 24, 36, 69, 93, 143, 175
Mva1269I GAATGC 1 cut(s) 115
NcoI CCATGG 1 cut(s) 140
NlaIII CATG 1 cut(s) 144
NlaIV GGNNCC 3 cut(s) 57, 127, 290
PctI GAATGC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 280
PflMI CCANNNNNTGG 2 cut(s) 146, 354
PkrI GCNGC 2 cut(s) 51, 310
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 3 cut(s) 57, 127, 290
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 3 cut(s) 55, 125, 126
PspPPI RGGWCCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 2 cut(s) 50, 309
Sau96I GGNCC 3 cut(s) 55, 125, 126
SduI GDGCHC 3 cut(s) 82, 129, 293
SetI ASST 5 cut(s) 47, 154, 158, 313, 333
SfcI CTRYAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 1 cut(s) 173
SsiI CCGC 2 cut(s) 75, 248
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 5, 223, 336
TasI AATT 1 cut(s) 173
TfiI GAWTC 1 cut(s) 280
TscAI CASTG 2 cut(s) 213, 290
TseI GCWGC 2 cut(s) 49, 308
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 2 cut(s) 213, 290
Van91I CCANNNNNTGG 2 cut(s) 146, 354
VpaK11BI GGWCC 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.