Rh4CG406500

Domain of unknown function (DUF1929)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008580
Physical Location & Seq
Forward (+)
65340908 .. 65341582
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG406500.1

Sequence Viewer

Length: 675 bp
ATGGCTTTATCATTATCCCCCATCTTCTTCATTCTTCTCTCCCTCTACGTCTCCCCCTCCTTTTACGCACATCCGAGCAAGGAAACTGCGGCTGAAACTCTTTCAGCCGTCGCTTCCAACGGAGGAGGAGAGTGGGTTCTTCTTCAAAAAAGCATCGGAGTCTCGGCAATGCACATGCAGCTTCTCAAGAACAACAGAGTCATCATCTTCGACCGCACCAACATCGGCCCTTCCAACCTCACACTCCCCGAGGACAAAAAGTACTGCCTCAACTACACCATCCACAACAAACCCGAGCTCCTTATAGACTGCACCGCGCACTCCGTCTCCTACGACGTCGCTTCCAACACTTTCCGCCCCCTCGTCGTCCGCACCGATCCGTGGTGTTCCTCCGGCTCCGTGGACCCTACAGGAACCCTAATCCAGACCGGAGGCTACGGCACCGGCAGCCGCGCCGTCCGCACGGTCCCGCCCTGCGACGACGACACTTGTGACTGGACGGAACATCAGCAAGACAGCCTCGCGGAGAACAGGTGGTACGCCTCGTCGCAAATCCTCCCCGACGGACGCGTCATCGTCGTCGGAGGCCGAAAAGCTTTCACGTACGAGTTCTATCCCAAAACAAACGACAGGTCGTCGGATAAATATTACCTAAAATTTTTGGTAGAGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

224

Amino Acids

24.87

Weight (kDa)

5.94

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 57 - 224 1.1e-64 Glyoxal oxidase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 569
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 440
AccII CGCG 4 cut(s) 317, 453, 524, 570
AciI CCGC 9 cut(s) 89, 214, 315, 355, 370, 451, 460, 470, 524
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 656
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 3 cut(s) 263, 539, 605
AfiI CCNNNNNNNGG 1 cut(s) 381
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 1 cut(s) 146
AhdI GACNNNNNGTC 1 cut(s) 634
AloI GAACNNNNNNTCC 2 cut(s) 120, 152
AluBI AGCT 3 cut(s) 181, 298, 596
AluI AGCT 3 cut(s) 181, 298, 596
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 4 cut(s) 55, 166, 331, 662
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 2 cut(s) 248, 293
AoxI GGCC 2 cut(s) 226, 586
ApeKI GCWGC 2 cut(s) 178, 447
ApoI RAATTY 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AspLEI GCGC 2 cut(s) 319, 455
AspS9I GGNCC 3 cut(s) 227, 403, 466
AvaI CYCGRG 2 cut(s) 248, 293
AvaII GGWCC 2 cut(s) 403, 466
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 190, 459
BccI CCATC 2 cut(s) 29, 287
BceAI ACGGC 3 cut(s) 92, 440, 454
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BcoDI GTCTC 4 cut(s) 55, 166, 331, 662
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 4 cut(s) 90, 179, 448, 451
BlsI GCNGC 4 cut(s) 91, 180, 449, 452
BmcAI AGTACT 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 403, 466
BmeRI GACNNNNNGTC 1 cut(s) 634
BmeT110I CYCGRG 2 cut(s) 248, 293
BmgT120I GGNCC 3 cut(s) 227, 403, 466
BmiI GGNNCC 5 cut(s) 397, 405, 415, 442, 468
BmsI GCATC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 3 cut(s) 249, 380, 399
BsaWI WCCGGW 1 cut(s) 428
BsaXI ACNNNNNCTCC 8 cut(s) 120, 150, 282, 311, 312, 341, 518, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 381
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 249, 380, 399
BseGI GGATG 2 cut(s) 70, 279
BseLI CCNNNNNNNGG 1 cut(s) 381
BseMI GCAATG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 500
BseRI GAGGAG 2 cut(s) 138, 141
BseXI GCAGC 2 cut(s) 190, 459
BsgI GTGCAG 1 cut(s) 295
Bsh1236I CGCG 4 cut(s) 317, 453, 524, 570
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 228, 588
BshNI GGYRCC 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 2 cut(s) 248, 293
BsiSI CCGG 3 cut(s) 393, 429, 444
BsiWI CGTACG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 381
BsmAI GTCTC 4 cut(s) 55, 166, 331, 662
BsmBI CGTCTC 3 cut(s) 55, 331, 662
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 2 cut(s) 228, 588
BsoBI CYCGRG 2 cut(s) 248, 293
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 9 cut(s) 89, 214, 315, 355, 370, 451, 460, 470, 524
BspANI GGCC 2 cut(s) 228, 588
BspFNI CGCG 4 cut(s) 317, 453, 524, 570
BspLI GGNNCC 5 cut(s) 397, 405, 415, 442, 468
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 249, 380, 399
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 466
BstACI GRCGYC 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 603
BstDSI CCRYGG 2 cut(s) 380, 399
BstF5I GGATG 2 cut(s) 70, 279
BstFNI CGCG 4 cut(s) 317, 453, 524, 570
BstHHI GCGC 2 cut(s) 319, 455
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 4 cut(s) 55, 166, 331, 662
BstMBI GATC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 178, 447, 459
BstNSI RCATGY 1 cut(s) 178
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 4 cut(s) 317, 453, 524, 570
BstV1I GCAGC 2 cut(s) 190, 459
BsuRI GGCC 2 cut(s) 228, 588
BtgI CCRYGG 2 cut(s) 380, 399
BtsCI GGATG 2 cut(s) 70, 279
CfoI GCGC 2 cut(s) 319, 455
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 3 cut(s) 227, 403, 466
CseI GACGC 2 cut(s) 559, 576
Csp6I GTAC 3 cut(s) 262, 538, 604
CviAII CATG 1 cut(s) 175
CviQI GTAC 3 cut(s) 262, 538, 604
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DrdI GACNNNNNNGTC 1 cut(s) 569
DriI GACNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 569
Eam1105I GACNNNNNGTC 1 cut(s) 634
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 403, 466
Eco53kI GAGCTC 1 cut(s) 298
Eco88I CYCGRG 2 cut(s) 248, 293
EcoICRI GAGCTC 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 300
Esp3I CGTCTC 3 cut(s) 55, 331, 662
FaeI CATG 1 cut(s) 178
FaiI YATR 2 cut(s) 176, 305
FaqI GGGAC 1 cut(s) 452
FatI CATG 1 cut(s) 174
FauI CCCGC 1 cut(s) 477
Fnu4HI GCNGC 4 cut(s) 90, 179, 448, 451
FokI GGATG 2 cut(s) 57, 266
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 4 cut(s) 90, 179, 448, 451
GlaI GCGC 2 cut(s) 318, 454
GluI GCNGC 4 cut(s) 90, 179, 448, 451
HaeIII GGCC 2 cut(s) 228, 588
HapII CCGG 3 cut(s) 393, 429, 444
HgaI GACGC 2 cut(s) 559, 576
HhaI GCGC 2 cut(s) 319, 455
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 2 cut(s) 317, 453
HinP1I GCGC 2 cut(s) 317, 453
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 2 cut(s) 159, 198
HpaII CCGG 3 cut(s) 393, 429, 444
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 4 cut(s) 75, 158, 584, 640
Hpy188III TCNNGA 2 cut(s) 187, 424
Hpy8I GTNNAC 1 cut(s) 403
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 4 cut(s) 48, 336, 602, 671
HpyCH4V TGCA 3 cut(s) 172, 178, 312
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 447, 459
HpySE526I ACGT 4 cut(s) 48, 336, 602, 671
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 2 cut(s) 317, 453
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 2 cut(s) 303, 401
LpnPI CCDG 9 cut(s) 396, 406, 437, 442, 457, 481, 487, 517, 616
Lsp1109I GCAGC 2 cut(s) 190, 459
LweI GCATC 1 cut(s) 162
MaeII ACGT 4 cut(s) 48, 336, 602, 671
MaeIII GTNAC 1 cut(s) 491
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 6 cut(s) 16, 19, 26, 131, 134, 199
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 1 cut(s) 656
MluI ACGCGT 1 cut(s) 568
MlyI GAGTC 2 cut(s) 168, 207
MmeI TCCRAC 5 cut(s) 141, 258, 369, 562, 618
MspI CCGG 3 cut(s) 393, 429, 444
MvnI CGCG 4 cut(s) 317, 453, 524, 570
MwoI GCNNNNNNNGC 3 cut(s) 178, 447, 459
NdeII GATC 1 cut(s) 376
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 5 cut(s) 397, 405, 415, 442, 468
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 372
Pfl23II CGTACG 1 cut(s) 603
PkrI GCNGC 4 cut(s) 91, 180, 449, 452
PleI GAGTC 2 cut(s) 167, 206
PpsI GAGTC 2 cut(s) 167, 206
Ppu21I YACGTR 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 300
PspLI CGTACG 1 cut(s) 603
PspN4I GGNNCC 5 cut(s) 397, 405, 415, 442, 468
PspPI GGNCC 3 cut(s) 227, 403, 466
PsrI GAACNNNNNNTAC 2 cut(s) 521, 553
RsaI GTAC 3 cut(s) 263, 539, 605
RsaNI GTAC 3 cut(s) 262, 538, 604
SacI GAGCTC 1 cut(s) 300
SatI GCNGC 4 cut(s) 90, 179, 448, 451
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 3 cut(s) 227, 403, 466
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 2 cut(s) 168, 207
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 2 cut(s) 403, 466
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 1 cut(s) 656
SsiI CCGC 9 cut(s) 89, 214, 315, 355, 370, 451, 460, 470, 524
SspI AATATT 1 cut(s) 647
SstI GAGCTC 1 cut(s) 300
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 4 cut(s) 51, 339, 605, 674
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 656
TatI WGTACW 1 cut(s) 261
TauI GCSGC 2 cut(s) 92, 453
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 2 cut(s) 178, 447
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 19
TspGWI ACGGA 6 cut(s) 135, 313, 369, 388, 515, 579
VpaK11BI GGWCC 2 cut(s) 403, 466
XapI RAATTY 1 cut(s) 656
XceI RCATGY 1 cut(s) 178
ZraI GACGTC 1 cut(s) 337
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.