Rh4DG072800

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018871
Physical Location & Seq
Forward (+)
10750197 .. 10750640
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG072800.1

Sequence Viewer

Length: 444 bp
ATGACTCAATATCATATGACATCTGCCGCCGGAACCTTCTGTTATATTGATCCAGAGTATCAGCAAACTGGCATGCTTGGGATAAAATCTGATATATACTCACTTGGGGTCATGTTCCTACAACTAATAACAGCCAAACCACCTATGGGTTTGACTCATCACGTTGAGCGGGCTATTGAGAAAGGGACTTTCTCTGAGATCCTTGACCCTGCCGTTCCTGACTGGCCAGTTGAGGAAGCATTGTGTTTTGCAAAGCTAGCTCTACAGTGTGCTGAAATGAGGCAAAAAGATAGACCGGATCTTGGGAAGATTGTTCTTCCTGAGCTTAACAGATTAAGAAAATTAGGCGAGGATAGCATGAATGGCGTTACGGTTGGTAATGGTGAAGTATCTTCAGCTAGACAAGTGAGTACCATAGCAGACCTAGCATTGCACTTGAAGTAA

Protein Analysis

147

Amino Acids

16.22

Weight (kDa)

5.52

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 101 8.4e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018871)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05410 FvH4_7g05450
rosa_chinensis RchiOBHm_Chr4g0397661
rosa_samantha Rh2AG371200 Rh2CG359500 Rh2DG382200 Rh4AG079600 Rh4CG085600 Rh4DG072800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 2 cut(s) 27, 169
AclWI GGATC 3 cut(s) 44, 193, 306
AcoI YGGCCR 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 146, 302
AgsI TTSAA 1 cut(s) 439
AluBI AGCT 4 cut(s) 256, 260, 325, 398
AluI AGCT 4 cut(s) 256, 260, 325, 398
AlwI GGATC 3 cut(s) 44, 193, 306
AoxI GGCC 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 226
BceAI ACGGC 1 cut(s) 197
BfaI CTAG 3 cut(s) 257, 399, 425
BfmI CTRYAG 1 cut(s) 263
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 34
BmtI GCTAGC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 302
Bse1I ACTGG 3 cut(s) 73, 227, 227
Bse3DI GCAATG 1 cut(s) 428
BseLI CCNNNNNNNGG 2 cut(s) 146, 302
BseMI GCAATG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 186, 312
BseNI ACTGG 3 cut(s) 73, 227, 227
BshFI GGCC 1 cut(s) 226
BsiSI CCGG 2 cut(s) 30, 296
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 146, 302
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 1 cut(s) 226
Bsp143I GATC 3 cut(s) 49, 198, 298
BspACI CCGC 2 cut(s) 27, 169
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 2 cut(s) 187, 313
BspLI GGNNCC 1 cut(s) 34
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 3 cut(s) 44, 193, 306
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 428
BsrI ACTGG 3 cut(s) 73, 227, 227
BssMI GATC 3 cut(s) 49, 198, 298
Bst4CI ACNGT 2 cut(s) 267, 373
BstC8I GCNNGC 3 cut(s) 74, 171, 258
BstDEI CTNAG 2 cut(s) 195, 321
BstKTI GATC 3 cut(s) 52, 201, 301
BstMBI GATC 3 cut(s) 49, 198, 298
BstMWI GCNNNNNNNGC 4 cut(s) 257, 354, 363, 425
BstNSI RCATGY 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 198, 298
BstYI RGATCY 2 cut(s) 198, 298
BsuRI GGCC 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 74, 171, 258
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 73, 112, 358
CviJI RGCY 7 cut(s) 134, 173, 226, 256, 260, 325, 398
CviKI_1 RGCY 7 cut(s) 134, 173, 226, 256, 260, 325, 398
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 195, 321
DpnI GATC 3 cut(s) 51, 200, 300
DpnII GATC 3 cut(s) 49, 198, 298
EaeI YGGCCR 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 378
FaeI CATG 3 cut(s) 76, 115, 361
FaqI GGGAC 1 cut(s) 199
FatI CATG 3 cut(s) 72, 111, 357
FauI CCCGC 1 cut(s) 162
FauNDI CATATG 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 3 cut(s) 257, 399, 425
GluI GCNGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 226
HapII CCGG 2 cut(s) 30, 296
Hin1II CATG 3 cut(s) 76, 115, 361
HinfI GANTC 2 cut(s) 4, 154
HpaII CCGG 2 cut(s) 30, 296
HphI GGTGA 1 cut(s) 395
Hpy188I TCNGA 2 cut(s) 91, 196
Hpy188III TCNNGA 3 cut(s) 53, 218, 320
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 267, 373
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 2 cut(s) 251, 433
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 354, 363, 425
HpyF3I CTNAG 2 cut(s) 195, 321
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 3 cut(s) 76, 115, 361
Kzo9I GATC 3 cut(s) 49, 198, 298
LpnPI CCDG 9 cut(s) 43, 54, 66, 208, 222, 231, 240, 309, 333
MaeI CTAG 3 cut(s) 257, 399, 425
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 367
MalI GATC 3 cut(s) 51, 200, 300
MbiI CCGCTC 1 cut(s) 169
MboI GATC 3 cut(s) 49, 198, 298
MboII GAAGA 3 cut(s) 308, 319, 384
MflI RGATCY 2 cut(s) 198, 298
MlsI TGGCCA 1 cut(s) 226
MluCI AATT 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 226
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 3 cut(s) 226, 273, 343
Mox20I TGGCCA 1 cut(s) 226
MscI TGGCCA 1 cut(s) 226
MseI TTAA 2 cut(s) 327, 335
Msp20I TGGCCA 1 cut(s) 226
MspI CCGG 2 cut(s) 30, 296
MwoI GCNNNNNNNGC 4 cut(s) 257, 354, 363, 425
NdeI CATATG 1 cut(s) 15
NdeII GATC 3 cut(s) 49, 198, 298
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 3 cut(s) 76, 115, 361
NlaIV GGNNCC 1 cut(s) 34
NspI RCATGY 1 cut(s) 76
PaeI GCATGC 1 cut(s) 76
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 198, 298
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SaqAI TTAA 2 cut(s) 327, 335
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 3 cut(s) 49, 198, 298
SchI GAGTC 1 cut(s) 148
SetI ASST 8 cut(s) 38, 145, 165, 258, 262, 327, 400, 426
SfcI CTRYAG 1 cut(s) 263
SphI GCATGC 1 cut(s) 76
Sse9I AATT 1 cut(s) 341
SsiI CCGC 2 cut(s) 27, 169
SspMI CTAG 3 cut(s) 257, 399, 425
TaaI ACNGT 2 cut(s) 267, 373
TaiI ACGT 1 cut(s) 165
TasI AATT 1 cut(s) 341
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 2 cut(s) 327, 335
Tru9I TTAA 2 cut(s) 327, 335
TscAI CASTG 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 1 cut(s) 272
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XspI CTAG 3 cut(s) 257, 399, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.