Rh5AG103300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012664
Physical Location & Seq
Forward (+)
9520782 .. 9521599
818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG103300.1

Sequence Viewer

Length: 723 bp
ATGGGAGATAGGCGACCCCTGCACTTCAATTTCTTCTCTTCCCTGAAGCAGGTTGAGAAGAGATTGAAACTAGAACACCCATCTGAACAATCTACTGTATTACCATCTCCATTGCTAGAAAGCAACAAGTTATTAACAGAATCACTAAGCTCACCCATATACCTTGATTTGGATCCTTCCAACAACAACAACCATTACAGTTTTAGTACACTCCAAGACAGTAGTGAAGCCCCTGAAGCATTCCTCTCATGCTCTCCACGGTTTATACCAACCCAAGAGAACCCAACTCAGCCAAATGCCCTGCATGACCCCAAAACTATCAATGACACTGGAGGTGAAGCCATCGTCGATGATATTGAACGGTTGATTCAACTCTTGGGTTTGTCAAGTTGCCAGGAAGGAGATGAAGAAAAGGCTGGTTTGGAGTTGAAGTGTGGAGTTAGTGAAAATGGGGGTAATTCTTGTCATTGTGAAGGCGGGTTTTATGAGAAGATTGTTGGGGTGAAGGGGCCAAAGTGTGGGAGAGAAGTGGAGAGGTTGGAGGGTTGGATTAACTACTTTTTGAATGGTAATGGGGAGGGAAAGATTGAGCCTTTCAGGTTGGCACATTTGCTTTTGAGTAAAGCTGCTTTTGTCTCTGATTGTGCTGATCATGGTTTTGGAGACTTGGACTTCCCTTCAACGATTGGGGATTTTCTGCGCACTGATCCTCCAACAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.41

Weight (kDa)

4.75

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 701
Acc36I ACCTGC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 518
AciI CCGC 1 cut(s) 477
AclWI GGATC 3 cut(s) 167, 180, 701
AcuI CTGAAG 2 cut(s) 65, 255
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 3 cut(s) 49, 169, 518
AgsI TTSAA 7 cut(s) 28, 67, 359, 371, 430, 565, 681
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 2 cut(s) 150, 626
AluI AGCT 2 cut(s) 150, 626
Alw26I GTCTC 2 cut(s) 640, 657
AlwI GGATC 3 cut(s) 167, 180, 701
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 626
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 144, 347, 514
BamHI GGATCC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 613
BccI CCATC 3 cut(s) 88, 112, 350
BciT130I CCWGG 1 cut(s) 395
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 2 cut(s) 640, 657
BfaI CTAG 2 cut(s) 71, 116
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
Bme1390I CCNGG 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 174, 510
BmrFI CCNGG 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 257
BsaXI ACNNNNNCTCC 3 cut(s) 416, 446, 694
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 169, 518
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 257
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 49, 169, 518
BseMI GCAATG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 334
BseXI GCAGC 1 cut(s) 613
BsgI GTGCAG 1 cut(s) 5
BshFI GGCC 1 cut(s) 511
BslI CCNNNNNNNGG 3 cut(s) 49, 169, 518
BsmAI GTCTC 2 cut(s) 640, 657
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 1 cut(s) 511
Bsp143I GATC 3 cut(s) 172, 649, 706
BspACI CCGC 1 cut(s) 477
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 174, 510
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 167, 180, 701
BsrDI GCAATG 1 cut(s) 110
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 3 cut(s) 172, 649, 706
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 5 cut(s) 97, 200, 221, 261, 363
Bst6I CTCTTC 2 cut(s) 43, 53
BstDEI CTNAG 2 cut(s) 146, 288
BstDSI CCRYGG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 3 cut(s) 175, 652, 709
BstMAI GTCTC 2 cut(s) 640, 657
BstMBI GATC 3 cut(s) 172, 649, 706
BstMWI GCNNNNNNNGC 2 cut(s) 19, 236
BstNI CCWGG 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuRI GGCC 1 cut(s) 511
BtgI CCRYGG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 327, 702
BveI ACCTGC 1 cut(s) 40
CfoI GCGC 1 cut(s) 702
Cfr13I GGNCC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 207
CviAII CATG 3 cut(s) 249, 305, 653
CviJI RGCY 8 cut(s) 150, 230, 292, 341, 416, 511, 592, 626
CviKI_1 RGCY 8 cut(s) 150, 230, 292, 341, 416, 511, 592, 626
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 2 cut(s) 146, 288
DpnI GATC 3 cut(s) 174, 651, 708
DpnII GATC 3 cut(s) 172, 649, 706
Eam1104I CTCTTC 2 cut(s) 43, 53
EarI CTCTTC 2 cut(s) 43, 53
Eco57I CTGAAG 2 cut(s) 65, 255
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 393
FaeI CATG 3 cut(s) 252, 308, 656
FaiI YATR 7 cut(s) 158, 160, 250, 266, 306, 486, 654
FatI CATG 3 cut(s) 248, 304, 652
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 1 cut(s) 627
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 2 cut(s) 71, 116
FspI TGCGCA 1 cut(s) 701
GlaI GCGC 1 cut(s) 701
GluI GCNGC 1 cut(s) 627
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 1 cut(s) 511
HhaI GCGC 1 cut(s) 702
Hin1II CATG 3 cut(s) 252, 308, 656
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HinfI GANTC 2 cut(s) 140, 367
HphI GGTGA 3 cut(s) 144, 347, 514
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 2 cut(s) 85, 640
Hpy8I GTNNAC 1 cut(s) 209
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 5 cut(s) 186, 392, 467, 499, 687
HpyCH4III ACNGT 5 cut(s) 97, 200, 221, 261, 363
HpyCH4V TGCA 2 cut(s) 22, 304
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 236
HpyF3I CTNAG 2 cut(s) 146, 288
Hsp92II CATG 3 cut(s) 252, 308, 656
HspAI GCGC 1 cut(s) 700
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 3 cut(s) 172, 649, 706
Lsp1109I GCAGC 1 cut(s) 613
MaeI CTAG 2 cut(s) 71, 116
MalI GATC 3 cut(s) 174, 651, 708
MboI GATC 3 cut(s) 172, 649, 706
MboII GAAGA 5 cut(s) 25, 30, 70, 419, 502
MflI RGATCY 1 cut(s) 172
MluCI AATT 2 cut(s) 28, 457
MmeI TCCRAC 3 cut(s) 204, 519, 527
MnlI CCTC 6 cut(s) 254, 326, 528, 535, 571, 720
MseI TTAA 3 cut(s) 134, 552, 721
MspR9I CCNGG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 19, 236
NdeII GATC 3 cut(s) 172, 649, 706
NlaIII CATG 3 cut(s) 252, 308, 656
NlaIV GGNNCC 2 cut(s) 174, 510
NsbI TGCGCA 1 cut(s) 701
PctI GAATGC 1 cut(s) 239
PfeI GAWTC 2 cut(s) 140, 367
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 1 cut(s) 628
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 174, 510
PspPI GGNCC 1 cut(s) 509
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
SaqAI TTAA 3 cut(s) 134, 552, 721
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 3 cut(s) 172, 649, 706
Sau96I GGNCC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 395
SetI ASST 7 cut(s) 54, 152, 165, 337, 539, 602, 628
Sse9I AATT 2 cut(s) 28, 457
SsiI CCGC 1 cut(s) 477
SspMI CTAG 2 cut(s) 71, 116
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 5 cut(s) 97, 200, 221, 261, 363
TaqI TCGA 1 cut(s) 348
TasI AATT 2 cut(s) 28, 457
TatI WGTACW 1 cut(s) 206
TfiI GAWTC 2 cut(s) 140, 367
Tru1I TTAA 3 cut(s) 134, 552, 721
Tru9I TTAA 3 cut(s) 134, 552, 721
TscAI CASTG 2 cut(s) 334, 709
TseI GCWGC 1 cut(s) 626
TspDTI ATGAA 1 cut(s) 420
TspRI CASTG 2 cut(s) 334, 709
Van91I CCANNNNNTGG 1 cut(s) 518
XagI CCTNNNNNAGG 1 cut(s) 47
XspI CTAG 2 cut(s) 71, 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.