Rh5AG146900

At4g19900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009629
Physical Location & Seq
Reverse (-)
15463832 .. 15470975
7144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG146900.1

Sequence Viewer

Length: 1494 bp
ATGCTGAGAAGCATCCGTTCCCGGCGGCCCCACCGCCCCCGTTACGGCGTGTACATCTGTGCCGTGATCTCCGCCCTCCTCCTCCTCCTCTCCGTCTCTCTCCTCTACACCCGCCTCTCCCACTCCCAATCCCACCACTTCAACTACCGCCACCCTTCCCCCATCTCCCAAAACGACGACGTTTCCCTCTCCAACCCCCTCATCTCCGACGACGCCACCGCCGCCGTCACCACCATCCCCGCCGACGACAAGATCGACGAGCTCGACGAGGTCGTCGTCGACGACGCCCCCAGAGACGACGAATTGGACGACGAAGACGATCCCCAGTCGGAAAAATCTCAGGGATCCTCCGGATTCTTCTTCGATCATGTCGGCGGGGTTATCAGAAGAGGCTTGAATAAGCGAAAGATTGAAGATTGGGACGAAGATTACAGTGGGTTTAGTGTAGGGTTGGGCGCGGTGGATAAAAGCATCGTGGCGTTTGGATCGGACGACGTGCCGGTGGATATGGAGGTGAGGAGGAGGATGACTGAGGTGGCGGGTGTGGAGGACGCGCTGATGGTGAAGGTGGGGAAGAGGGTTTCGCCGCTGAGGGAGGGGTGGGGGGAGTGGTTTGATAAGAAGAGTGATTTTCTGAGGAGGGATAAGATGTTCAAGTCCAATTTGGAGCTGTTGAACCCTCTGCATAACCCAATGCTGCAAGACCCCGATGCCGTCGGGGTTTCCGGGTTAACTAAGGGAGACAAGGCTGTGCAGAAATGGTGGCTGAGCCACTTTAAGAAGGTGCCGTTTCGGAGGAGGAAGGAGAGTGGGAAGAAGGAGAGTGGCGGCGGAGATGGTGGTGTGAATGTGAGTGGGAGAGGTGTGGGAGTGGAGTTGAGTGAGGTTGAGAGAGCTGAGAGGAAGACTTTGGATGAGAATGGAGGTAAAGTTTTGATTCCTGGTGGGAGTGGGAATGCTAGTGGTGATAGGAATTCATCGAATGTTGGTAATGTGAGTGAGGTAGCAGTTGGGAGAACTGTTGAACAGATTAGTGAATCGGTTCAGGTTGGTGGAATTAAGAATGAGTTTTCGGGTCTTATTTATGCGGATGGCAAGAGATGGGGTTTCTATTCCGATTTGGATCCCTATTTGTCATTTACGGATTTTATGGATGCGTTTTTTAGCAAGGGGTGTGATTTGAGGGTGTTTATGGTGTGGAATTCTCCAGCTTGGATGTTTAGTGTCCGGCACCAGCGGGGGCTTGAGAGTTTACTCTCCCATCATCGACATGCCTGTGTTGTGGTGTTTTCTGAGACAATTGAGCTTGATTTCTTTAAAAATAGCTTTGTAAAAGACGGTTACAAAGTTGCTGTTGCTATGCCAAATCTTGACGAGTTACTAAAGGGTACACCTACTCATATATTTGCTTCTGCCTGGTTCGAATGGAGAAAGACAAAGCATTATGCTACTCATTACAGTGAGCTTATCCGCCTTGCTGCTCTTTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

497

Amino Acids

55.63

Weight (kDa)

6.05

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 409 - 497 7.3e-07 Glycosyltransferase sugar-binding region containing DXD motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AccB1I GGYRCC 2 cut(s) 784, 1230
AccI GTMKAC 1 cut(s) 279
AccII CGCG 2 cut(s) 458, 554
AccIII TCCGGA 1 cut(s) 350
AclWI GGATC 6 cut(s) 314, 339, 352, 493, 1118, 1131
AcsI RAATTY 2 cut(s) 973, 1201
AcyI GRCGYC 2 cut(s) 213, 285
AfaI GTAC 2 cut(s) 53, 1390
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 6 cut(s) 142, 397, 413, 655, 676, 1025
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 2 cut(s) 940, 1415
AloI GAACNNNNNNTCC 2 cut(s) 635, 667
AluBI AGCT 7 cut(s) 262, 670, 896, 1211, 1306, 1326, 1465
AluI AGCT 7 cut(s) 262, 670, 896, 1211, 1306, 1326, 1465
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 4 cut(s) 100, 288, 735, 1289
AlwI GGATC 6 cut(s) 314, 339, 352, 493, 1118, 1131
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 697, 1478
ApoI RAATTY 2 cut(s) 973, 1201
Asp700I GAANNNNTTC 1 cut(s) 1041
AspLEI GCGC 2 cut(s) 458, 556
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 2 cut(s) 22, 727
AsuHPI GGTGA 4 cut(s) 220, 526, 574, 977
AsuII TTCGAA 1 cut(s) 1422
BamHI GGATCC 2 cut(s) 344, 1123
BanI GGYRCC 2 cut(s) 784, 1230
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 321, 911
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 590
BbvI GCAGC 2 cut(s) 684, 1465
BccI CCATC 7 cut(s) 170, 242, 553, 830, 1085, 1095, 1269
BceAI ACGGC 5 cut(s) 47, 61, 209, 698, 772
BciT130I CCWGG 2 cut(s) 942, 1417
BcnI CCSGG 2 cut(s) 22, 727
BcoDI GTCTC 4 cut(s) 100, 288, 735, 1289
BfaI CTAG 1 cut(s) 960
BisI GCNGC 6 cut(s) 26, 222, 587, 698, 829, 1479
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 6 cut(s) 27, 223, 588, 699, 830, 1480
Bme1390I CCNGG 4 cut(s) 22, 727, 942, 1417
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 5 cut(s) 29, 346, 786, 1125, 1232
BmrFI CCNGG 4 cut(s) 22, 727, 942, 1417
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 4 cut(s) 21, 480, 700, 1144
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 2 cut(s) 321, 911
BpmI CTGGAG 1 cut(s) 1191
Bpu10I CCTNAGC 1 cut(s) 590
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 1422
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 2 cut(s) 22, 727
BsaBI GATNNNNATC 1 cut(s) 1122
BsaHI GRCGYC 2 cut(s) 213, 285
BsaWI WCCGGW 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 915, 945
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 1122
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 2 cut(s) 942, 1417
BseGI GGATG 7 cut(s) 12, 234, 531, 919, 1096, 1159, 1221
BseJI GATNNNNATC 1 cut(s) 1122
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 7 cut(s) 353, 522, 581, 626, 758, 888, 1284
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 9 cut(s) 68, 71, 74, 77, 92, 532, 535, 652, 811
BseXI GCAGC 2 cut(s) 684, 1465
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 2 cut(s) 458, 554
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 2 cut(s) 784, 1230
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 5 cut(s) 22, 351, 500, 726, 1228
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 4 cut(s) 100, 288, 735, 1289
BsmBI CGTCTC 2 cut(s) 100, 288
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 1 cut(s) 28
Bsp119I TTCGAA 1 cut(s) 1422
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
Bsp1720I GCTNAGC 1 cut(s) 767
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 7 cut(s) 352, 523, 582, 627, 759, 889, 1285
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 2 cut(s) 458, 554
BspLI GGNNCC 5 cut(s) 29, 346, 786, 1125, 1232
BspPI GGATC 6 cut(s) 314, 339, 352, 493, 1118, 1131
BspT104I TTCGAA 1 cut(s) 1422
BspT107I GGYRCC 2 cut(s) 784, 1230
BsrFI RCCGGY 1 cut(s) 499
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 499
BssMI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
BssNI GRCGYC 2 cut(s) 213, 285
Bst2UI CCWGG 2 cut(s) 942, 1417
Bst4CI ACNGT 4 cut(s) 434, 1021, 1340, 1460
Bst6I CTCTTC 3 cut(s) 382, 569, 617
BstACI GRCGYC 2 cut(s) 213, 285
BstAUI TGTACA 1 cut(s) 51
BstBI TTCGAA 1 cut(s) 1422
BstDEI CTNAG 9 cut(s) 5, 339, 531, 590, 635, 735, 767, 897, 1293
BstF5I GGATG 7 cut(s) 12, 234, 531, 919, 1096, 1159, 1221
BstFNI CGCG 2 cut(s) 458, 554
BstHHI GCGC 2 cut(s) 458, 556
BstKTI GATC 7 cut(s) 69, 255, 322, 347, 367, 488, 1126
BstMAI GTCTC 4 cut(s) 100, 288, 735, 1289
BstMBI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNI CCWGG 2 cut(s) 942, 1417
BstNSI RCATGY 1 cut(s) 1274
BstSCI CCNGG 4 cut(s) 20, 725, 940, 1415
BstUI CGCG 2 cut(s) 458, 554
BstV1I GCAGC 2 cut(s) 684, 1465
BstV2I GAAGAC 2 cut(s) 321, 911
BstX2I RGATCY 2 cut(s) 344, 1123
BstYI RGATCY 2 cut(s) 344, 1123
BsuRI GGCC 1 cut(s) 28
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 7 cut(s) 12, 234, 531, 919, 1096, 1159, 1221
BtsIMutI CAGTG 2 cut(s) 439, 1465
CfoI GCGC 2 cut(s) 458, 556
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 3 cut(s) 221, 293, 560
Csp6I GTAC 2 cut(s) 52, 1389
CviAII CATG 2 cut(s) 368, 1271
CviQI GTAC 2 cut(s) 52, 1389
DdeI CTNAG 9 cut(s) 5, 339, 531, 590, 635, 735, 767, 897, 1293
DpnI GATC 7 cut(s) 68, 254, 321, 346, 366, 487, 1125
DpnII GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
DraI TTTAAA 1 cut(s) 1318
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
Eam1104I CTCTTC 3 cut(s) 382, 569, 617
EarI CTCTTC 3 cut(s) 382, 569, 617
EciI GGCGGA 3 cut(s) 61, 846, 1460
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRI GAATTC 2 cut(s) 973, 1201
EcoRII CCWGG 2 cut(s) 940, 1415
EcoT38I GRGCYC 1 cut(s) 264
Esp3I CGTCTC 2 cut(s) 100, 288
FaeI CATG 2 cut(s) 371, 1274
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 367, 1270
FauI CCCGC 5 cut(s) 119, 247, 368, 532, 1230
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 26, 222, 587, 698, 829, 1479
FokI GGATG 6 cut(s) 221, 538, 926, 1103, 1166, 1228
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 6 cut(s) 26, 222, 587, 698, 829, 1479
FspBI CTAG 1 cut(s) 960
GlaI GCGC 2 cut(s) 457, 555
GluI GCNGC 6 cut(s) 26, 222, 587, 698, 829, 1479
GsuI CTGGAG 1 cut(s) 1191
HaeIII GGCC 1 cut(s) 28
HapII CCGG 5 cut(s) 22, 351, 500, 726, 1228
HgaI GACGC 3 cut(s) 221, 293, 560
HhaI GCGC 2 cut(s) 458, 556
Hin1I GRCGYC 2 cut(s) 213, 285
Hin1II CATG 2 cut(s) 371, 1274
Hin6I GCGC 2 cut(s) 456, 554
HinP1I GCGC 2 cut(s) 456, 554
HincII GTYRAC 2 cut(s) 280, 732
HindII GTYRAC 2 cut(s) 280, 732
HinfI GANTC 3 cut(s) 354, 937, 1037
HpaI GTTAAC 1 cut(s) 732
HpaII CCGG 5 cut(s) 22, 351, 500, 726, 1228
HphI GGTGA 4 cut(s) 220, 526, 574, 977
Hpy166II GTNNAC 5 cut(s) 52, 280, 732, 1253, 1391
Hpy188I TCNGA 8 cut(s) 208, 331, 386, 490, 636, 795, 1117, 1294
Hpy188III TCNNGA 2 cut(s) 351, 1370
Hpy8I GTNNAC 5 cut(s) 52, 280, 732, 1253, 1391
HpyAV CCTTC 5 cut(s) 165, 559, 775, 796, 811
HpyCH4III ACNGT 4 cut(s) 434, 1021, 1340, 1460
HpyCH4IV ACGT 2 cut(s) 180, 495
HpyCH4V TGCA 3 cut(s) 685, 700, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 9 cut(s) 5, 339, 531, 590, 635, 735, 767, 897, 1293
HpySE526I ACGT 2 cut(s) 180, 495
Hsp92I GRCGYC 2 cut(s) 213, 285
Hsp92II CATG 2 cut(s) 371, 1274
HspAI GCGC 2 cut(s) 456, 554
Kpn2I TCCGGA 1 cut(s) 350
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 684, 1465
LweI GCATC 4 cut(s) 21, 480, 700, 1144
MaeI CTAG 1 cut(s) 960
MaeII ACGT 2 cut(s) 180, 495
MaeIII GTNAC 4 cut(s) 41, 226, 1340, 1377
MalI GATC 7 cut(s) 68, 254, 321, 346, 366, 487, 1125
MboI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
MfeI CAATTG 1 cut(s) 1299
MflI RGATCY 2 cut(s) 344, 1123
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 6 cut(s) 302, 661, 973, 1056, 1201, 1299
MmeI TCCRAC 3 cut(s) 216, 231, 309
MroI TCCGGA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 1041
MseI TTAA 4 cut(s) 731, 777, 1059, 1317
MslI CAYNNNNRTG 3 cut(s) 1269, 1275, 1458
MspA1I CMGCKG 2 cut(s) 589, 1237
MspI CCGG 5 cut(s) 22, 351, 500, 726, 1228
MspR9I CCNGG 4 cut(s) 22, 727, 942, 1417
MunI CAATTG 1 cut(s) 1299
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 2 cut(s) 942, 1417
MvnI CGCG 2 cut(s) 458, 554
MwoI GCNNNNNNNGC 1 cut(s) 221
NciI CCSGG 2 cut(s) 22, 727
NdeII GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
NlaIII CATG 2 cut(s) 371, 1274
NlaIV GGNNCC 5 cut(s) 29, 346, 786, 1125, 1232
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 1274
NspV TTCGAA 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 8 cut(s) 252, 261, 264, 270, 273, 279, 306, 315
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 1 cut(s) 1041
PfeI GAWTC 3 cut(s) 354, 937, 1037
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 6 cut(s) 27, 223, 588, 699, 830, 1480
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 2 cut(s) 940, 1415
PspGI CCWGG 2 cut(s) 940, 1415
PspN4I GGNNCC 5 cut(s) 29, 346, 786, 1125, 1232
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 2 cut(s) 344, 1123
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 2 cut(s) 53, 1390
RsaNI GTAC 2 cut(s) 52, 1389
RseI CAYNNNNRTG 3 cut(s) 1269, 1275, 1458
SacI GAGCTC 1 cut(s) 264
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 4 cut(s) 731, 777, 1059, 1317
SatI GCNGC 6 cut(s) 26, 222, 587, 698, 829, 1479
Sau3AI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1123
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 4 cut(s) 22, 727, 942, 1417
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 4 cut(s) 21, 480, 700, 1144
SfuI TTCGAA 1 cut(s) 1422
SgrDI CGTCGACG 1 cut(s) 278
SmiMI CAYNNNNRTG 3 cut(s) 1269, 1275, 1458
SmlI CTYRAG 1 cut(s) 1244
SmoI CTYRAG 1 cut(s) 1244
Sse9I AATT 6 cut(s) 302, 661, 973, 1056, 1201, 1299
SspMI CTAG 1 cut(s) 960
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 4 cut(s) 20, 725, 940, 1415
TaaI ACNGT 4 cut(s) 434, 1021, 1340, 1460
TaiI ACGT 2 cut(s) 183, 498
TaqI TCGA 7 cut(s) 255, 264, 279, 363, 980, 1267, 1422
TasI AATT 6 cut(s) 302, 661, 973, 1056, 1201, 1299
TatI WGTACW 1 cut(s) 51
TauI GCSGC 4 cut(s) 28, 224, 589, 831
TfiI GAWTC 3 cut(s) 354, 937, 1037
Tru1I TTAA 4 cut(s) 731, 777, 1059, 1317
Tru9I TTAA 4 cut(s) 731, 777, 1059, 1317
TscAI CASTG 2 cut(s) 439, 1465
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 697, 1478
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 966
TspGWI ACGGA 3 cut(s) 5, 82, 1157
TspRI CASTG 2 cut(s) 439, 1465
Tth111I GACNNNGTC 1 cut(s) 269
XapI RAATTY 2 cut(s) 973, 1201
XceI RCATGY 1 cut(s) 1274
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 1041
XspI CTAG 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.