Rh5DG145900

At4g19900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009629
Physical Location & Seq
Reverse (-)
14903964 .. 14911183
7220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG145900.1

Sequence Viewer

Length: 1644 bp
ATGCTGAGAAGCATCCGTTCCCGGCGGCCCCACCGCCCCCGTTACGGCGTGTACATCTGTGCCGTGATCTCCGCCCTCCTCCTCCTCCTCTCCGTCTCTCTCCTCTACACCCGCCTCTCCCACTCCCAATCCCACCACTTCAACTACCGCCACCCTTCCCCCATCTCCCAAAACGACGACGTTTCCCTCTCCAACCCCCTCATCTCCGACGACGCCACCGCCGCCGTCACCACCATCCCCTCCGACGACAAGATCGACGAGCTCGATGAGGTCGTCGTCGACGACGCCCCCAGAGACGACGAATTGGACGACGACGACGATCCCCAGTCCGAAAAATCTCAGGGATCCTCTGGATTCTTCTTCGATCATGTCGGCGGGGTTATCAGAAGAGGCTTGAATAAGCGAAAGATTGAAGATTGGGACGAAGATTACAGTGGGTTTAGTGTAGGGTTGGGCGCGGTGGATAAAAGCATCGTGGCGTTTGGATCGGACGACGTGCCGGTGGATATGGAGGTGAGGAGGAGGATGACTGAGGTGGCGGGTGTGGAGGACGCGCTGATGGTGAAGGTGGGGAAGAGGGTTTCGCCGCTGAGGGAGGGGTGGGGGGAGTGGTTTGATAAGAAGAGTGATTTTCTGAGGAGGGATAAGATGTTCAAGTCCAATTTGGAGCTGTTGAACCCTCTGCATAACCCAATGCTGCAAGACCCCGATGCCGTCGGGGTTTCCGGGTTAACTAAGGGAGACAAGGCTGTGCAGAAATGGTGGCTGAGCCACTTTAAGAAGGTGCCGTTTCGGAGGAGGAAGGAGAGTGGTGGTGGTGAGAGTGGGAAGAAGGAGAGTGGCGGCAGAGATGGTGGTGTGAATGTGAGTGGGAGAGGTGTGGGAGTGGAGTTGAGTGAGGTTGAGAGAGCTGAGAGGAAGACTTTGGATGAGAATGGAGGTAAACTTTTGATTCCCGGTGGGAGTGGGAATGCTAGTGGTGATAGGAATTCATCGAATGTTGGTAATGTGAGTGAGGTAGCAGTTGGGAGAACTGTTGAACAGATTAGTGAATCGGTTCAGGTTGGTGGAATTAAGAATGAGTTTTCGGGTCTTATTTATGCGGATGGCAAGAGATGGGGTTTCTATTCCGATTTGGATCCCTATTTGTCATTTACGGATTTTATGGATGCGTTTTTTAGCAAGGGGTGTGATTTGAGGGTGTTTATGGTGTGGAATTCTCCAGCTTGGATGTTTAGTGTCCGGCACCAGCGGGGGCTTGAGAGTTTACTCTCCCATCATCGACATGCCTGTGTTGTGGTGTTTTCTGAGACAATTGAGCTTGATTTCTTTAAAAATAGCTTTGTAAAAGACGGTTACAAAGTTGCTGTTGCTATGCCAAATCTTGACGAGTTACTAAAGGGTACACCTACTCATATATTTGCTTCTGCCTGGTTCGAATGGAGAAAGACAAAGCATTATGCTACTCATTACAGTGAGCTTATCCGCCTTGCTGCTCTTTACAAATACGGCGGAATATATCTTGATTCTGATATCATAGTTCTGAAATCGCTATCTTCACTCAGCAATTGTGTTGGTAAAGAAGATAGACTAGCAGGAGGTTCTTTGAATGGTGCTGTAATGGCATTTAAAAGGAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

547

Amino Acids

60.92

Weight (kDa)

6.34

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 414 - 523 2.8e-19 Glycosyltransferase sugar-binding region containing DXD motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 784, 1245
AccI GTMKAC 1 cut(s) 279
AccII CGCG 2 cut(s) 458, 554
AclWI GGATC 6 cut(s) 314, 339, 352, 493, 1133, 1146
AcsI RAATTY 2 cut(s) 988, 1216
AcyI GRCGYC 2 cut(s) 213, 285
AfaI GTAC 2 cut(s) 53, 1405
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 7 cut(s) 142, 397, 413, 655, 676, 1040, 1609
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 1 cut(s) 1430
AloI GAACNNNNNNTCC 2 cut(s) 635, 667
AluBI AGCT 7 cut(s) 262, 670, 911, 1226, 1321, 1341, 1480
AluI AGCT 7 cut(s) 262, 670, 911, 1226, 1321, 1341, 1480
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 4 cut(s) 100, 288, 735, 1304
AlwI GGATC 6 cut(s) 314, 339, 352, 493, 1133, 1146
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 697, 1493
ApoI RAATTY 2 cut(s) 988, 1216
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 458, 556
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 3 cut(s) 22, 727, 957
AsuHPI GGTGA 5 cut(s) 220, 526, 574, 830, 992
AsuII TTCGAA 1 cut(s) 1437
BamHI GGATCC 2 cut(s) 344, 1138
BanI GGYRCC 2 cut(s) 784, 1245
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 926
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 590
BbvI GCAGC 2 cut(s) 684, 1480
BccI CCATC 7 cut(s) 170, 242, 553, 845, 1100, 1110, 1284
BceAI ACGGC 6 cut(s) 47, 61, 209, 698, 772, 1525
BciT130I CCWGG 1 cut(s) 1432
BcnI CCSGG 3 cut(s) 22, 727, 957
BcoDI GTCTC 4 cut(s) 100, 288, 735, 1304
BfaI CTAG 2 cut(s) 975, 1592
BisI GCNGC 6 cut(s) 26, 222, 587, 698, 844, 1494
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 6 cut(s) 27, 223, 588, 699, 845, 1495
Bme1390I CCNGG 4 cut(s) 22, 727, 957, 1432
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 5 cut(s) 29, 346, 786, 1140, 1247
BmrFI CCNGG 4 cut(s) 22, 727, 957, 1432
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 4 cut(s) 21, 480, 700, 1159
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 926
BpmI CTGGAG 1 cut(s) 1206
Bpu10I CCTNAGC 1 cut(s) 590
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 1437
BpuEI CTTGAG 1 cut(s) 1280
BpuMI CCSGG 3 cut(s) 22, 727, 957
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 2 cut(s) 213, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 1 cut(s) 1432
BseGI GGATG 7 cut(s) 12, 234, 531, 934, 1111, 1174, 1236
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 8 cut(s) 353, 522, 581, 626, 758, 903, 1299, 1576
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 9 cut(s) 68, 71, 74, 77, 92, 532, 535, 652, 811
BseXI GCAGC 2 cut(s) 684, 1480
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 2 cut(s) 458, 554
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 2 cut(s) 784, 1245
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 5 cut(s) 22, 500, 726, 957, 1243
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 4 cut(s) 100, 288, 735, 1304
BsmBI CGTCTC 2 cut(s) 100, 288
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 1 cut(s) 28
Bsp119I TTCGAA 1 cut(s) 1437
Bsp1286I GDGCHC 1 cut(s) 264
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
Bsp1720I GCTNAGC 1 cut(s) 767
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 8 cut(s) 352, 523, 582, 627, 759, 904, 1300, 1575
BspFNI CGCG 2 cut(s) 458, 554
BspLI GGNNCC 5 cut(s) 29, 346, 786, 1140, 1247
BspPI GGATC 6 cut(s) 314, 339, 352, 493, 1133, 1146
BspT104I TTCGAA 1 cut(s) 1437
BspT107I GGYRCC 2 cut(s) 784, 1245
BsrFI RCCGGY 1 cut(s) 499
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 499
BssMI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
BssNI GRCGYC 2 cut(s) 213, 285
Bst2UI CCWGG 1 cut(s) 1432
Bst4CI ACNGT 4 cut(s) 434, 1036, 1355, 1475
Bst6I CTCTTC 3 cut(s) 382, 569, 617
BstACI GRCGYC 2 cut(s) 213, 285
BstAUI TGTACA 1 cut(s) 51
BstBI TTCGAA 1 cut(s) 1437
BstF5I GGATG 7 cut(s) 12, 234, 531, 934, 1111, 1174, 1236
BstFNI CGCG 2 cut(s) 458, 554
BstHHI GCGC 2 cut(s) 458, 556
BstKTI GATC 7 cut(s) 69, 255, 322, 347, 367, 488, 1141
BstMAI GTCTC 4 cut(s) 100, 288, 735, 1304
BstMBI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
BstMWI GCNNNNNNNGC 2 cut(s) 221, 1622
BstNI CCWGG 1 cut(s) 1432
BstNSI RCATGY 1 cut(s) 1289
BstSCI CCNGG 4 cut(s) 20, 725, 955, 1430
BstUI CGCG 2 cut(s) 458, 554
BstV1I GCAGC 2 cut(s) 684, 1480
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 2 cut(s) 344, 1138
BstYI RGATCY 2 cut(s) 344, 1138
BsuRI GGCC 1 cut(s) 28
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 7 cut(s) 12, 234, 531, 934, 1111, 1174, 1236
BtsIMutI CAGTG 2 cut(s) 439, 1480
CfoI GCGC 2 cut(s) 458, 556
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 3 cut(s) 221, 293, 560
Csp6I GTAC 2 cut(s) 52, 1404
CviAII CATG 2 cut(s) 368, 1286
CviQI GTAC 2 cut(s) 52, 1404
DpnI GATC 7 cut(s) 68, 254, 321, 346, 366, 487, 1140
DpnII GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
DraI TTTAAA 2 cut(s) 1333, 1630
Eam1104I CTCTTC 3 cut(s) 382, 569, 617
EarI CTCTTC 3 cut(s) 382, 569, 617
EciI GGCGGA 3 cut(s) 61, 1475, 1527
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 1534
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRI GAATTC 2 cut(s) 988, 1216
EcoRII CCWGG 1 cut(s) 1430
EcoRV GATATC 1 cut(s) 1534
EcoT38I GRGCYC 1 cut(s) 264
Esp3I CGTCTC 2 cut(s) 100, 288
FaeI CATG 2 cut(s) 371, 1289
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 367, 1285
FauI CCCGC 4 cut(s) 119, 368, 532, 1245
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 26, 222, 587, 698, 844, 1494
FokI GGATG 6 cut(s) 221, 538, 941, 1118, 1181, 1243
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 6 cut(s) 26, 222, 587, 698, 844, 1494
FspBI CTAG 2 cut(s) 975, 1592
GlaI GCGC 2 cut(s) 457, 555
GluI GCNGC 6 cut(s) 26, 222, 587, 698, 844, 1494
GsuI CTGGAG 1 cut(s) 1206
HaeIII GGCC 1 cut(s) 28
HapII CCGG 5 cut(s) 22, 500, 726, 957, 1243
HgaI GACGC 3 cut(s) 221, 293, 560
HhaI GCGC 2 cut(s) 458, 556
Hin1I GRCGYC 2 cut(s) 213, 285
Hin1II CATG 2 cut(s) 371, 1289
Hin6I GCGC 2 cut(s) 456, 554
HinP1I GCGC 2 cut(s) 456, 554
HincII GTYRAC 2 cut(s) 280, 732
HindII GTYRAC 2 cut(s) 280, 732
HinfI GANTC 4 cut(s) 354, 952, 1052, 1526
HpaI GTTAAC 1 cut(s) 732
HpaII CCGG 5 cut(s) 22, 500, 726, 957, 1243
HphI GGTGA 5 cut(s) 220, 526, 574, 830, 992
Hpy166II GTNNAC 6 cut(s) 52, 280, 732, 944, 1268, 1406
Hpy188III TCNNGA 3 cut(s) 351, 1385, 1523
Hpy8I GTNNAC 6 cut(s) 52, 280, 732, 944, 1268, 1406
HpyAV CCTTC 5 cut(s) 165, 559, 775, 796, 826
HpyCH4III ACNGT 4 cut(s) 434, 1036, 1355, 1475
HpyCH4IV ACGT 2 cut(s) 180, 495
HpyCH4V TGCA 3 cut(s) 685, 700, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 1622
HpySE526I ACGT 2 cut(s) 180, 495
Hsp92I GRCGYC 2 cut(s) 213, 285
Hsp92II CATG 2 cut(s) 371, 1289
HspAI GCGC 2 cut(s) 456, 554
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 684, 1480
LweI GCATC 4 cut(s) 21, 480, 700, 1159
MaeI CTAG 2 cut(s) 975, 1592
MaeII ACGT 2 cut(s) 180, 495
MaeIII GTNAC 4 cut(s) 41, 226, 1355, 1392
MalI GATC 7 cut(s) 68, 254, 321, 346, 366, 487, 1140
MboI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
MfeI CAATTG 2 cut(s) 1314, 1567
MflI RGATCY 2 cut(s) 344, 1138
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 7 cut(s) 302, 661, 988, 1071, 1216, 1314, 1567
MmeI TCCRAC 3 cut(s) 216, 231, 267
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 5 cut(s) 731, 777, 1074, 1332, 1629
MslI CAYNNNNRTG 3 cut(s) 1284, 1290, 1473
MspA1I CMGCKG 2 cut(s) 589, 1252
MspI CCGG 5 cut(s) 22, 500, 726, 957, 1243
MspR9I CCNGG 4 cut(s) 22, 727, 957, 1432
MunI CAATTG 2 cut(s) 1314, 1567
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 1 cut(s) 1432
MvnI CGCG 2 cut(s) 458, 554
MwoI GCNNNNNNNGC 2 cut(s) 221, 1622
NciI CCSGG 3 cut(s) 22, 727, 957
NdeII GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
NlaIII CATG 2 cut(s) 371, 1289
NlaIV GGNNCC 5 cut(s) 29, 346, 786, 1140, 1247
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 1289
NspV TTCGAA 1 cut(s) 1437
PcsI WCGNNNNNNNCGW 6 cut(s) 252, 261, 270, 279, 306, 315
PctI GAATGC 1 cut(s) 976
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 4 cut(s) 354, 952, 1052, 1526
PkrI GCNGC 6 cut(s) 27, 223, 588, 699, 845, 1495
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 1430
PspGI CCWGG 1 cut(s) 1430
PspN4I GGNNCC 5 cut(s) 29, 346, 786, 1140, 1247
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 2 cut(s) 344, 1138
RsaI GTAC 2 cut(s) 53, 1405
RsaNI GTAC 2 cut(s) 52, 1404
RseI CAYNNNNRTG 3 cut(s) 1284, 1290, 1473
SacI GAGCTC 1 cut(s) 264
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 5 cut(s) 731, 777, 1074, 1332, 1629
SatI GCNGC 6 cut(s) 26, 222, 587, 698, 844, 1494
Sau3AI GATC 7 cut(s) 66, 252, 319, 344, 364, 485, 1138
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 4 cut(s) 22, 727, 957, 1432
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 4 cut(s) 21, 480, 700, 1159
SfuI TTCGAA 1 cut(s) 1437
SgrDI CGTCGACG 1 cut(s) 278
SmiMI CAYNNNNRTG 3 cut(s) 1284, 1290, 1473
SmlI CTYRAG 1 cut(s) 1259
SmoI CTYRAG 1 cut(s) 1259
Sse9I AATT 7 cut(s) 302, 661, 988, 1071, 1216, 1314, 1567
SspMI CTAG 2 cut(s) 975, 1592
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 4 cut(s) 20, 725, 955, 1430
TaaI ACNGT 4 cut(s) 434, 1036, 1355, 1475
TaiI ACGT 2 cut(s) 183, 498
TaqI TCGA 7 cut(s) 255, 264, 279, 363, 995, 1282, 1437
TasI AATT 7 cut(s) 302, 661, 988, 1071, 1216, 1314, 1567
TatI WGTACW 1 cut(s) 51
TauI GCSGC 4 cut(s) 28, 224, 589, 846
TfiI GAWTC 4 cut(s) 354, 952, 1052, 1526
Tru1I TTAA 5 cut(s) 731, 777, 1074, 1332, 1629
Tru9I TTAA 5 cut(s) 731, 777, 1074, 1332, 1629
TscAI CASTG 2 cut(s) 439, 1480
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 697, 1493
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 981
TspGWI ACGGA 3 cut(s) 5, 82, 1172
TspRI CASTG 2 cut(s) 439, 1480
XapI RAATTY 2 cut(s) 988, 1216
XceI RCATGY 1 cut(s) 1289
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 2 cut(s) 975, 1592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.