Rh5AG394100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018611
Physical Location & Seq
Forward (+)
68267834 .. 68269451
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG394100.1

Sequence Viewer

Length: 1320 bp
ATGTTCGATTCACAATTTGCAGCATGGCTGAACAGATATCATGAGGTTAATGAGCAGGTACAGGTTGAGATAAGTTTAACGGTGAGAACTGCATATGGTTTATCAATTAGTGCTCATGGAAATATTCCAAATTGGTTCAACAATAGAAGCAGCAACTTTTCTATAACAATCCCGTTTTCAAAGGACAGAACTAATAAGAGTAGGCTATGGATGGGGTGTGCTTTCTATGTTGTTTTGGAAGTTGATGAGAATTTTGATACGGGTTCTGATTTCAAAGTAAGGCACAAGTTTGACTGTTGTTTTGATACCATGGAAGGTCCTGGTTACAAACAACCTCTATACCACGAGGTAAATGCTAAAGGTGTCCATTTGACTGGGTCAATTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGTTGAATGCCTTCAATCAGTGCGGTAGGATCGAGGCTAACACTACAATTGATAGCCCATGTGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTCTACGAAAAAAAAGATTTTAGAGGGTTTGTCCAAGCGATAAAAAGGGTGATTCGGGTTCCAAGAATTAGGCAGAATAACAGTTCCTCTGTAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGCATTTCGTTGAAAGAACTTCTTAGCTTGCTTCCTTCAGATTTAGAATGCTATGAGGGGCAATGCGAGGGGGGATTCGAGTACCATATTGGAGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGATATATTCTGGTCAGCACGCCGTGTATTATGAAGAAACTTTTCCTCAATATGGAATTCCAGAATGGTTCTGCAGTGTGAATGGTTCGTCGGCAGCCATCCCGCTAAATCCTATGAATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAGTTTGGATCCTGAAAAGACTCGCATACGCTGTAGTTTGGAGACTGGCTTTCGGAGTATCACAATATCTGGACGCATCACTAAATCTCTTACTAGTTTGAAGGTTCGTGGTTACGTGTGTATGTTTTATGTATCGCGTGGGTCACTTTCACGTGTTATGTCAAATCATTACACTTCCAACATCAAAGCCAGGTTTTATAGTACCAGAGAGGACATAACGGTGCAGAGTTGTGGGTATCAAATTGTGACCCATGAAAATATGGAAGAATTTATGGAAACAATGCTGCAATGTTCAATCACATCTCAGACTTCTCTGTTGTCAAAAGATACAAGTAGAGAAAAGATGTTCTCTAAATGCTTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

439

Amino Acids

49.66

Weight (kDa)

7.5

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 514
AccII CGCG 1 cut(s) 1090
AciI CCGC 3 cut(s) 404, 447, 893
AclWI GGATC 3 cut(s) 461, 956, 969
AcsI RAATTY 3 cut(s) 250, 846, 1220
AcuI CTGAAG 2 cut(s) 669, 773
AcvI CACGTG 1 cut(s) 1106
AdeI CACNNNGTG 1 cut(s) 814
AfaI GTAC 3 cut(s) 60, 731, 1156
AfiI CCNNNNNNNGG 3 cut(s) 487, 842, 908
AflIII ACRYGT 2 cut(s) 1068, 1105
AgsI TTSAA 8 cut(s) 139, 180, 274, 430, 439, 661, 1054, 1248
AhlI ACTAGT 1 cut(s) 1046
AjnI CCWGG 2 cut(s) 319, 1142
AluBI AGCT 3 cut(s) 633, 675, 926
AluI AGCT 3 cut(s) 633, 675, 926
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 774, 989
AlwI GGATC 3 cut(s) 461, 956, 969
Ama87I CYCGRG 1 cut(s) 646
ApeKI GCWGC 6 cut(s) 20, 150, 633, 884, 926, 1237
ApoI RAATTY 3 cut(s) 250, 846, 1220
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 3 cut(s) 434, 786, 831
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 94, 577
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 961
BanI GGYRCC 1 cut(s) 506
BauI CACGAG 1 cut(s) 344
BbrPI CACGTG 1 cut(s) 1106
Bbv12I GWGCWC 1 cut(s) 115
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 6 cut(s) 32, 162, 620, 896, 913, 1224
BccI CCATC 3 cut(s) 205, 620, 896
BceAI ACGGC 2 cut(s) 797, 920
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 2 cut(s) 321, 1144
BcoDI GTCTC 2 cut(s) 774, 989
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 1 cut(s) 1047
BfmI CTRYAG 2 cut(s) 862, 985
BfuAI ACCTGC 1 cut(s) 46
BisI GCNGC 6 cut(s) 21, 151, 634, 885, 927, 1238
BlsI GCNGC 6 cut(s) 22, 152, 635, 886, 928, 1239
Bme1390I CCNGG 2 cut(s) 321, 1144
Bme18I GGWCC 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 3 cut(s) 508, 576, 963
BmrFI CCNGG 2 cut(s) 321, 1144
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 415, 1038
BmuI ACTGGG 1 cut(s) 384
Bpu10I CCTNAGC 1 cut(s) 621
BsaAI YACGTR 2 cut(s) 1069, 1106
BsaJI CCNNGG 1 cut(s) 309
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 842, 908
Bse1I ACTGG 2 cut(s) 379, 1003
Bse3DI GCAATG 2 cut(s) 716, 1247
BseBI CCWGG 2 cut(s) 321, 1144
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 4 cut(s) 216, 631, 754, 888
BseLI CCNNNNNNNGG 3 cut(s) 487, 842, 908
BseMI GCAATG 2 cut(s) 716, 1247
BseMII CTCAG 2 cut(s) 612, 1271
BseNI ACTGG 2 cut(s) 379, 1003
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 6 cut(s) 32, 162, 620, 896, 913, 1224
BsgI GTGCAG 1 cut(s) 1196
Bsh1236I CGCG 1 cut(s) 1090
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 646
BslI CCNNNNNNNGG 3 cut(s) 487, 842, 908
BsmAI GTCTC 2 cut(s) 774, 989
BsmBI CGTCTC 1 cut(s) 774
BsmI GAATGC 2 cut(s) 436, 701
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 115, 511
Bsp143I GATC 2 cut(s) 453, 961
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 3 cut(s) 404, 447, 893
BspCNI CTCAG 2 cut(s) 613, 1270
BspFNI CGCG 1 cut(s) 1090
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 3 cut(s) 508, 576, 963
BspMAI CTGCAG 1 cut(s) 866
BspMI ACCTGC 1 cut(s) 46
BspPI GGATC 3 cut(s) 461, 956, 969
BspT107I GGYRCC 1 cut(s) 506
BsrDI GCAATG 2 cut(s) 716, 1247
BsrI ACTGG 2 cut(s) 379, 1003
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 2 cut(s) 453, 961
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 321, 1144
Bst4CI ACNGT 6 cut(s) 82, 296, 599, 946, 956, 1174
BstBAI YACGTR 2 cut(s) 1069, 1106
BstC8I GCNNGC 2 cut(s) 677, 810
BstDEI CTNAG 3 cut(s) 621, 671, 1257
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 4 cut(s) 216, 631, 754, 888
BstFNI CGCG 1 cut(s) 1090
BstKTI GATC 2 cut(s) 456, 964
BstMAI GTCTC 2 cut(s) 774, 989
BstMBI GATC 2 cut(s) 453, 961
BstMWI GCNNNNNNNGC 1 cut(s) 932
BstNI CCWGG 2 cut(s) 321, 1144
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 2 cut(s) 319, 1142
BstSFI CTRYAG 2 cut(s) 862, 985
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 1090
BstV1I GCAGC 6 cut(s) 32, 162, 620, 896, 913, 1224
BstX2I RGATCY 1 cut(s) 961
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 1 cut(s) 961
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 4 cut(s) 216, 631, 754, 888
BtsI GCAGTG 1 cut(s) 871
BtsIMutI CAGTG 2 cut(s) 449, 871
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 2 cut(s) 677, 810
CciI TCATGA 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 317
CseI GACGC 1 cut(s) 1035
Csp6I GTAC 3 cut(s) 59, 730, 1155
CviAII CATG 7 cut(s) 24, 41, 116, 310, 483, 930, 1205
CviQI GTAC 3 cut(s) 59, 730, 1155
DdeI CTNAG 3 cut(s) 621, 671, 1257
DpnI GATC 2 cut(s) 455, 963
DpnII GATC 2 cut(s) 453, 961
DraIII CACNNNGTG 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 309
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 317
Eco57I CTGAAG 2 cut(s) 669, 773
Eco72I CACGTG 1 cut(s) 1106
Eco88I CYCGRG 1 cut(s) 646
EcoO109I RGGNCCY 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 2 cut(s) 319, 1142
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 774
FaeI CATG 7 cut(s) 27, 44, 119, 313, 486, 933, 1208
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FatI CATG 7 cut(s) 23, 40, 115, 309, 482, 929, 1204
FauI CCCGC 1 cut(s) 900
FauNDI CATATG 1 cut(s) 94
FblI GTMKAC 1 cut(s) 514
Fnu4HI GCNGC 6 cut(s) 21, 151, 634, 885, 927, 1238
FokI GGATG 4 cut(s) 223, 638, 741, 875
Fsp4HI GCNGC 6 cut(s) 21, 151, 634, 885, 927, 1238
FspBI CTAG 1 cut(s) 1047
GluI GCNGC 6 cut(s) 21, 151, 634, 885, 927, 1238
HgaI GACGC 1 cut(s) 1035
Hin1II CATG 7 cut(s) 27, 44, 119, 313, 486, 933, 1208
HinfI GANTC 5 cut(s) 8, 568, 644, 723, 973
HphI GGTGA 2 cut(s) 94, 577
Hpy166II GTNNAC 1 cut(s) 515
Hpy188I TCNGA 5 cut(s) 268, 688, 792, 1008, 1260
Hpy188III TCNNGA 4 cut(s) 41, 851, 965, 1023
Hpy8I GTNNAC 1 cut(s) 515
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 5 cut(s) 308, 445, 693, 797, 1048
HpyCH4III ACNGT 6 cut(s) 82, 296, 599, 946, 956, 1174
HpyCH4IV ACGT 2 cut(s) 1068, 1105
HpyCH4V TGCA 7 cut(s) 20, 92, 636, 864, 929, 1177, 1240
HpyF10VI GCNNNNNNNGC 1 cut(s) 932
HpyF3I CTNAG 3 cut(s) 621, 671, 1257
HpySE526I ACGT 2 cut(s) 1068, 1105
Hsp92II CATG 7 cut(s) 27, 44, 119, 313, 486, 933, 1208
Kzo9I GATC 2 cut(s) 453, 961
Lsp1109I GCAGC 6 cut(s) 32, 162, 620, 896, 913, 1224
LweI GCATC 2 cut(s) 415, 1038
MaeI CTAG 1 cut(s) 1047
MaeII ACGT 2 cut(s) 1068, 1105
MaeIII GTNAC 4 cut(s) 323, 1064, 1095, 1198
MalI GATC 2 cut(s) 455, 963
MboI GATC 2 cut(s) 453, 961
MboII GAAGA 2 cut(s) 836, 1229
MfeI CAATTG 2 cut(s) 381, 471
MflI RGATCY 1 cut(s) 961
MhlI GDGCHC 2 cut(s) 115, 511
MlyI GAGTC 2 cut(s) 638, 967
MmeI TCCRAC 1 cut(s) 1155
MroXI GAANNNNTTC 3 cut(s) 434, 786, 831
MseI TTAA 3 cut(s) 48, 77, 495
MslI CAYNNNNRTG 1 cut(s) 1172
MspR9I CCNGG 2 cut(s) 321, 1144
MunI CAATTG 2 cut(s) 381, 471
Mva1269I GAATGC 2 cut(s) 436, 701
MvaI CCWGG 2 cut(s) 321, 1144
MvnI CGCG 1 cut(s) 1090
MwoI GCNNNNNNNGC 1 cut(s) 932
NcoI CCATGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 94
NdeII GATC 2 cut(s) 453, 961
NlaIII CATG 7 cut(s) 27, 44, 119, 313, 486, 933, 1208
NlaIV GGNNCC 3 cut(s) 508, 576, 963
NmuCI GTSAC 2 cut(s) 1095, 1198
NspI RCATGY 1 cut(s) 933
PaeR7I CTCGAG 1 cut(s) 646
PagI TCATGA 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 653
PctI GAATGC 2 cut(s) 436, 701
PdmI GAANNNNTTC 3 cut(s) 434, 786, 831
PfeI GAWTC 3 cut(s) 8, 568, 723
PflFI GACNNNGTC 2 cut(s) 376, 766
PkrI GCNGC 6 cut(s) 22, 152, 635, 886, 928, 1239
PleI GAGTC 2 cut(s) 638, 967
PmaCI CACGTG 1 cut(s) 1106
PmlI CACGTG 1 cut(s) 1106
PpsI GAGTC 2 cut(s) 638, 967
Ppu21I YACGTR 2 cut(s) 1069, 1106
PpuMI RGGWCCY 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 319, 1142
PspCI CACGTG 1 cut(s) 1106
PspGI CCWGG 2 cut(s) 319, 1142
PspN4I GGNNCC 3 cut(s) 508, 576, 963
PspPI GGNCC 1 cut(s) 317
PspPPI RGGWCCY 1 cut(s) 317
PspXI VCTCGAGB 1 cut(s) 646
PstI CTGCAG 1 cut(s) 866
PsuI RGATCY 1 cut(s) 961
PsyI GACNNNGTC 2 cut(s) 376, 766
RsaI GTAC 3 cut(s) 60, 731, 1156
RsaNI GTAC 3 cut(s) 59, 730, 1155
RseI CAYNNNNRTG 1 cut(s) 1172
SaqAI TTAA 3 cut(s) 48, 77, 495
SatI GCNGC 6 cut(s) 21, 151, 634, 885, 927, 1238
Sau3AI GATC 2 cut(s) 453, 961
Sau96I GGNCC 1 cut(s) 317
SchI GAGTC 2 cut(s) 638, 967
ScrFI CCNGG 2 cut(s) 321, 1144
SduI GDGCHC 2 cut(s) 115, 511
SfaNI GCATC 2 cut(s) 415, 1038
SfcI CTRYAG 2 cut(s) 862, 985
Sfr274I CTCGAG 1 cut(s) 646
SinI GGWCC 1 cut(s) 317
SlaI CTCGAG 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 1172
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SpeI ACTAGT 1 cut(s) 1046
SsiI CCGC 3 cut(s) 404, 447, 893
SspI AATATT 1 cut(s) 124
SspMI CTAG 1 cut(s) 1047
StyD4I CCNGG 2 cut(s) 319, 1142
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 6 cut(s) 82, 296, 599, 946, 956, 1174
TaiI ACGT 2 cut(s) 1071, 1108
TaqI TCGA 4 cut(s) 6, 456, 647, 726
TfiI GAWTC 3 cut(s) 8, 568, 723
Tru1I TTAA 3 cut(s) 48, 77, 495
Tru9I TTAA 3 cut(s) 48, 77, 495
TscAI CASTG 2 cut(s) 449, 871
TseFI GTSAC 2 cut(s) 1095, 1198
TseI GCWGC 6 cut(s) 20, 150, 633, 884, 926, 1237
Tsp45I GTSAC 2 cut(s) 1095, 1198
TspDTI ATGAA 4 cut(s) 743, 837, 920, 1221
TspRI CASTG 2 cut(s) 449, 871
Tth111I GACNNNGTC 2 cut(s) 376, 766
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 3 cut(s) 250, 846, 1220
XceI RCATGY 1 cut(s) 933
XhoI CTCGAG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 514
XmnI GAANNNNTTC 3 cut(s) 434, 786, 831
XspI CTAG 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.