Rh5AG394300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018611
Physical Location & Seq
Forward (+)
68303983 .. 68306058
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG394300.1

Sequence Viewer

Length: 1701 bp
ATGGAGTTGAGAGTAGAGGGAAACAATTTTACAAGCATACCTGAAAGTATTTGCCGACTTTCTAAGCTTGTAACACTTGAGTTGAATGGTTGTATCCTGTTGCAGTCATTGCCAGAGCTTCCATTGAGTATAAAATTGGTTTCTGCATATGGTTGTATGTCACTTAAAAGTGATTCTGATCAGTATGAAGTATATCGTTCAAAATATGATAACCATGTTCAGATTCATAAGCGCACAACATCAACCAGATTTTCCGTTCTCATAACCAATTCACAATTTGCAGCATGGCTGAACAGATATCATGAGGTTAATGAGGTACAGGTCGAGATAAGTTTACCGGTGAGAACTGCATATGGTTTATCACATAGAACTCATGGAAATATTCCATATTGGTTCAACAATAGAAGCGGCAACTTTTCTATAACAATCCCGCTTTCAGGGGACTTGACTAATAAGAGTAGGCTATGGATGGGATGTGCTTTCTATGTCGGTTTGGAAGTTAATGAGAATTTTGATAGCAGTCCATATTTCAAAGTCAGGCACAAGTTTGATTGTTGTTTTGATACCATGGAAGGTCCTGGTGACACACAAACTCTATACCACGAGGTAAATGCTAAAGGTGTCCATTTGACTGGGTCAATTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGCTGAATGCCTTCAATCAGTGCAGTAGCATTGAGGCTAACACTACAATTGATAGCCCATACGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTGTACAAAAGAACAGATTTTATAGAGTTTATCCAAGCAATAAAATCGGCGACTCAACTATCAAGAATTATGCAGAATAACAGTTCCTCTATAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGCATTTTGTTGAAAGAACATCTTAGCTCGCTGCCTTCAGATTTAGAATGCTATGAGGGGCACGCCTATGCGGGAAGAAATTACTACGTTATTGAAGGCAAAGTCGTTCATCCCCATACCTGGTCTCTGTTAGGAGAAATCCTTCAGAAATATTCTGGTCAGCACTCGGTGTTTTATAGAGTAACTTTTCCTCAATATGGAATTCCAGAATGGTTCTGCAGTGTGAATAGTTCCTCGGCAGCCTTCCCGCTAAATCCTATGAATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAATTTGGATGCTGAAAAGCCTGACATAGGCTTTAATTTGAAAATTGGCGGTCGGAATATCAGTGGACACATCACTGAATCCCTTACTAGTTTGAAGGTTGGTGGTTTCATGGGTATGTTTTATGCATCACGTGGGTCACTTTCACATCTTATGTCATCTGATTGCACTTCCACCACCAAAGTCAGTTTTTATAGTAACAGCAAGAACATAACGGTACAGAGTTGTGGGTATCAAATTGTATTCCATGAAAATATGGAAGAATTTATGGAAACAATACTGCAATGTTCAGTCGCATCGCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAAGGGAACCCTCTCTACCCAAACAGAAAAGGAGAAAAGATACCAGTAGAGAAGAAACACCGAAAGAAAATGAGAACTCTCTCTACCCGAATTTTGAAGTCTCCCTGTAGATCGATCTGTTGCTTCCTGTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

566

Amino Acids

63.8

Weight (kDa)

8.41

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccI GTMKAC 1 cut(s) 772
AciI CCGC 6 cut(s) 408, 431, 662, 977, 1154, 1263
AcsI RAATTY 4 cut(s) 508, 1107, 1475, 1653
AcuI CTGAAG 2 cut(s) 927, 1034
AcvI CACGTG 1 cut(s) 1346
AdeI CACNNNGTG 1 cut(s) 1075
AfaI GTAC 3 cut(s) 318, 779, 1431
AfiI CCNNNNNNNGG 6 cut(s) 437, 745, 1026, 1103, 1169, 1241
AgeI ACCGGT 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 1301
AjnI CCWGG 2 cut(s) 577, 1025
AluBI AGCT 5 cut(s) 67, 118, 891, 933, 1187
AluI AGCT 5 cut(s) 67, 118, 891, 933, 1187
Alw26I GTCTC 2 cut(s) 1035, 1668
Ama87I CYCGRG 1 cut(s) 904
ApeKI GCWGC 5 cut(s) 281, 891, 937, 1145, 1187
ApoI RAATTY 4 cut(s) 508, 1107, 1475, 1653
AsiGI ACCGGT 1 cut(s) 337
Asp700I GAANNNNTTC 2 cut(s) 692, 1047
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 352, 593
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 769, 969
BanI GGYRCC 1 cut(s) 764
BauI CACGAG 1 cut(s) 602
BbrPI CACGTG 1 cut(s) 1346
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 5 cut(s) 293, 878, 924, 1157, 1174
BccI CCATC 2 cut(s) 463, 878
BceAI ACGGC 1 cut(s) 1181
BcgI CGANNNNNNTGC 4 cut(s) 593, 627, 801, 835
BciT130I CCWGG 2 cut(s) 579, 1027
BciVI GTATCC 1 cut(s) 104
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 1035, 1668
BcuI ACTAGT 1 cut(s) 1301
BfaI CTAG 1 cut(s) 1302
BfmI CTRYAG 2 cut(s) 1123, 1669
BfuI GTATCC 1 cut(s) 104
BisI GCNGC 6 cut(s) 282, 409, 892, 938, 1146, 1188
BlsI GCNGC 6 cut(s) 283, 410, 893, 939, 1147, 1189
Bme1390I CCNGG 2 cut(s) 579, 1027
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 904
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 2 cut(s) 766, 1571
BmrFI CCNGG 2 cut(s) 579, 1027
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 4 cut(s) 673, 1213, 1349, 1517
BmuI ACTGGG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 98
Bsa29I ATCGAT 1 cut(s) 1676
BsaAI YACGTR 2 cut(s) 745, 1346
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 1035
BsaJI CCNNGG 2 cut(s) 567, 1140
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 6 cut(s) 437, 745, 1026, 1103, 1169, 1241
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 637, 1607
Bse3DI GCAATG 2 cut(s) 107, 1502
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 2 cut(s) 579, 1027
BseCI ATCGAT 1 cut(s) 1676
BseDI CCNNGG 2 cut(s) 567, 1140
BseGI GGATG 5 cut(s) 474, 479, 889, 1015, 1228
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 6 cut(s) 437, 745, 1026, 1103, 1169, 1241
BseMI GCAATG 2 cut(s) 107, 1502
BseMII CTCAG 2 cut(s) 870, 1535
BseNI ACTGG 2 cut(s) 637, 1607
BseSI GKGCMC 2 cut(s) 769, 969
BseXI GCAGC 5 cut(s) 293, 878, 924, 1157, 1174
BsgI GTGCAG 1 cut(s) 724
Bsh1285I CGRYCG 1 cut(s) 1267
BshNI GGYRCC 1 cut(s) 764
BshTI ACCGGT 1 cut(s) 337
BshVI ATCGAT 1 cut(s) 1676
BsiEI CGRYCG 1 cut(s) 1267
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 6 cut(s) 437, 745, 1026, 1103, 1169, 1241
BsmAI GTCTC 2 cut(s) 1035, 1668
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 2 cut(s) 694, 959
Bso31I GGTCTC 1 cut(s) 1035
BsoBI CYCGRG 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 769, 969
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 3 cut(s) 178, 1673, 1677
Bsp19I CCATGG 1 cut(s) 567
BspACI CCGC 6 cut(s) 408, 431, 662, 977, 1154, 1263
BspCNI CTCAG 2 cut(s) 871, 1534
BspDI ATCGAT 1 cut(s) 1676
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 766, 1571
BspMAI CTGCAG 1 cut(s) 1127
BspT107I GGYRCC 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 1035
BsrDI GCAATG 2 cut(s) 107, 1502
BsrFI RCCGGY 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 2 cut(s) 637, 1607
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 567, 1140
BssMI GATC 3 cut(s) 178, 1673, 1677
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 2 cut(s) 579, 1027
Bst4CI ACNGT 4 cut(s) 777, 857, 1207, 1429
BstAPI GCANNNNNTGC 1 cut(s) 109
BstAUI TGTACA 1 cut(s) 777
BstBAI YACGTR 2 cut(s) 745, 1346
BstC8I GCNNGC 2 cut(s) 935, 969
BstDEI CTNAG 4 cut(s) 63, 879, 929, 1521
BstDSI CCRYGG 1 cut(s) 567
BstENI CCTNNNNNAGG 1 cut(s) 1239
BstF5I GGATG 5 cut(s) 474, 479, 889, 1015, 1228
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 3 cut(s) 181, 1676, 1680
BstMAI GTCTC 2 cut(s) 1035, 1668
BstMBI GATC 3 cut(s) 178, 1673, 1677
BstMCI CGRYCG 1 cut(s) 1267
BstMWI GCNNNNNNNGC 2 cut(s) 109, 1193
BstNI CCWGG 2 cut(s) 579, 1027
BstNSI RCATGY 1 cut(s) 1194
BstSCI CCNGG 2 cut(s) 577, 1025
BstSFI CTRYAG 2 cut(s) 1123, 1669
BstSLI GKGCMC 2 cut(s) 769, 969
BstV1I GCAGC 5 cut(s) 293, 878, 924, 1157, 1174
BstXI CCANNNNNNTGG 1 cut(s) 632
Bsu15I ATCGAT 1 cut(s) 1676
BsuI GTATCC 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 1676
BtgI CCRYGG 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 1494
BtsCI GGATG 5 cut(s) 474, 479, 889, 1015, 1228
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 4 cut(s) 707, 1132, 1282, 1287
Cac8I GCNNGC 2 cut(s) 935, 969
CciI TCATGA 1 cut(s) 301
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 575
ClaI ATCGAT 1 cut(s) 1676
CsiI ACCWGGT 1 cut(s) 1025
Csp6I GTAC 3 cut(s) 317, 778, 1430
CspAI ACCGGT 1 cut(s) 337
CviAII CATG 8 cut(s) 215, 285, 302, 374, 568, 1191, 1324, 1460
CviQI GTAC 3 cut(s) 317, 778, 1430
DdeI CTNAG 4 cut(s) 63, 879, 929, 1521
DpnI GATC 3 cut(s) 180, 1675, 1679
DpnII GATC 3 cut(s) 178, 1673, 1677
DraIII CACNNNGTG 1 cut(s) 1075
Eco130I CCWWGG 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 1035
Eco32I GATATC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 927, 1034
Eco72I CACGTG 1 cut(s) 1346
Eco88I CYCGRG 1 cut(s) 904
EcoNI CCTNNNNNAGG 1 cut(s) 1239
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 1107
EcoRII CCWGG 2 cut(s) 577, 1025
EcoRV GATATC 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 1342
ErhI CCWWGG 1 cut(s) 567
FaeI CATG 8 cut(s) 218, 288, 305, 377, 571, 1194, 1327, 1463
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 1 cut(s) 455
FatI CATG 8 cut(s) 214, 284, 301, 373, 567, 1190, 1323, 1459
FauI CCCGC 3 cut(s) 438, 970, 1161
FauNDI CATATG 2 cut(s) 148, 352
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 1 cut(s) 772
Fnu4HI GCNGC 6 cut(s) 282, 409, 892, 938, 1146, 1188
FokI GGATG 5 cut(s) 481, 486, 896, 1002, 1235
Fsp4HI GCNGC 6 cut(s) 282, 409, 892, 938, 1146, 1188
FspBI CTAG 1 cut(s) 1302
GlaI GCGC 1 cut(s) 233
GluI GCNGC 6 cut(s) 282, 409, 892, 938, 1146, 1188
HapII CCGG 1 cut(s) 338
HhaI GCGC 1 cut(s) 234
Hin1II CATG 8 cut(s) 218, 288, 305, 377, 571, 1194, 1327, 1463
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 5 cut(s) 173, 223, 826, 902, 1292
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 352, 593
Hpy166II GTNNAC 3 cut(s) 335, 773, 1280
Hpy188I TCNGA 6 cut(s) 178, 222, 946, 1053, 1269, 1375
Hpy188III TCNNGA 4 cut(s) 302, 325, 837, 1112
Hpy8I GTNNAC 3 cut(s) 335, 773, 1280
HpyAV CCTTC 7 cut(s) 566, 703, 951, 995, 1058, 1159, 1303
HpyCH4III ACNGT 4 cut(s) 777, 857, 1207, 1429
HpyCH4IV ACGT 3 cut(s) 744, 993, 1345
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 1193
HpyF3I CTNAG 4 cut(s) 63, 879, 929, 1521
HpySE526I ACGT 3 cut(s) 744, 993, 1345
Hsp92II CATG 8 cut(s) 218, 288, 305, 377, 571, 1194, 1327, 1463
HspAI GCGC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 3 cut(s) 178, 1673, 1677
Lsp1109I GCAGC 5 cut(s) 293, 878, 924, 1157, 1174
LweI GCATC 4 cut(s) 673, 1213, 1349, 1517
MabI ACCWGGT 1 cut(s) 1025
MaeI CTAG 1 cut(s) 1302
MaeII ACGT 3 cut(s) 744, 993, 1345
MaeIII GTNAC 6 cut(s) 70, 159, 581, 1087, 1350, 1409
MalI GATC 3 cut(s) 180, 1675, 1679
MboI GATC 3 cut(s) 178, 1673, 1677
MboII GAAGA 4 cut(s) 993, 1484, 1559, 1627
MfeI CAATTG 2 cut(s) 639, 729
MhlI GDGCHC 2 cut(s) 769, 969
MlyI GAGTC 2 cut(s) 820, 896
MmeI TCCRAC 1 cut(s) 1247
Mph1103I ATGCAT 1 cut(s) 1342
MroXI GAANNNNTTC 2 cut(s) 692, 1047
MseI TTAA 5 cut(s) 165, 309, 501, 753, 1248
MslI CAYNNNNRTG 2 cut(s) 972, 1328
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 2 cut(s) 579, 1027
MunI CAATTG 2 cut(s) 639, 729
Mva1269I GAATGC 2 cut(s) 694, 959
MvaI CCWGG 2 cut(s) 579, 1027
MwoI GCNNNNNNNGC 2 cut(s) 109, 1193
NcoI CCATGG 1 cut(s) 567
NdeI CATATG 2 cut(s) 148, 352
NdeII GATC 3 cut(s) 178, 1673, 1677
NlaIII CATG 8 cut(s) 218, 288, 305, 377, 571, 1194, 1327, 1463
NlaIV GGNNCC 2 cut(s) 766, 1571
NmeAIII GCCGAG 1 cut(s) 1121
NmuCI GTSAC 3 cut(s) 159, 581, 1350
NsiI ATGCAT 1 cut(s) 1342
NspI RCATGY 1 cut(s) 1194
PaeR7I CTCGAG 1 cut(s) 904
PagI TCATGA 1 cut(s) 301
PctI GAATGC 2 cut(s) 694, 959
PdmI GAANNNNTTC 2 cut(s) 692, 1047
PfeI GAWTC 3 cut(s) 173, 223, 1292
PflFI GACNNNGTC 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 337
PkrI GCNGC 6 cut(s) 283, 410, 893, 939, 1147, 1189
PleI GAGTC 2 cut(s) 820, 896
PmaCI CACGTG 1 cut(s) 1346
PmlI CACGTG 1 cut(s) 1346
PpsI GAGTC 2 cut(s) 820, 896
Ppu21I YACGTR 2 cut(s) 745, 1346
PpuMI RGGWCCY 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 2 cut(s) 577, 1025
PspCI CACGTG 1 cut(s) 1346
PspGI CCWGG 2 cut(s) 577, 1025
PspN4I GGNNCC 2 cut(s) 766, 1571
PspPI GGNCC 1 cut(s) 575
PspPPI RGGWCCY 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 904
PsrI GAACNNNNNNTAC 4 cut(s) 1562, 1594, 1630, 1662
PstI CTGCAG 1 cut(s) 1127
PsyI GACNNNGTC 1 cut(s) 634
RsaI GTAC 3 cut(s) 318, 779, 1431
RsaNI GTAC 3 cut(s) 317, 778, 1430
RseI CAYNNNNRTG 2 cut(s) 972, 1328
SaqAI TTAA 5 cut(s) 165, 309, 501, 753, 1248
SatI GCNGC 6 cut(s) 282, 409, 892, 938, 1146, 1188
Sau3AI GATC 3 cut(s) 178, 1673, 1677
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 2 cut(s) 820, 896
ScrFI CCNGG 2 cut(s) 579, 1027
SduI GDGCHC 2 cut(s) 769, 969
SexAI ACCWGGT 1 cut(s) 1025
SfaNI GCATC 4 cut(s) 673, 1213, 1349, 1517
SfcI CTRYAG 2 cut(s) 1123, 1669
Sfr274I CTCGAG 1 cut(s) 904
SinI GGWCC 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 904
SmiMI CAYNNNNRTG 2 cut(s) 972, 1328
SmlI CTYRAG 2 cut(s) 77, 904
SmoI CTYRAG 2 cut(s) 77, 904
SpeI ACTAGT 1 cut(s) 1301
SsiI CCGC 6 cut(s) 408, 431, 662, 977, 1154, 1263
SspI AATATT 2 cut(s) 382, 1058
SspMI CTAG 1 cut(s) 1302
StyD4I CCNGG 2 cut(s) 577, 1025
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 4 cut(s) 777, 857, 1207, 1429
TaiI ACGT 3 cut(s) 747, 996, 1348
TaqI TCGA 3 cut(s) 324, 905, 1676
TatI WGTACW 1 cut(s) 777
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 3 cut(s) 173, 223, 1292
Tru1I TTAA 5 cut(s) 165, 309, 501, 753, 1248
Tru9I TTAA 5 cut(s) 165, 309, 501, 753, 1248
TscAI CASTG 4 cut(s) 707, 1132, 1282, 1294
TseFI GTSAC 3 cut(s) 159, 581, 1350
TseI GCWGC 5 cut(s) 281, 891, 937, 1145, 1187
Tsp45I GTSAC 3 cut(s) 159, 581, 1350
TspDTI ATGAA 6 cut(s) 201, 215, 1004, 1181, 1312, 1476
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 4 cut(s) 707, 1132, 1282, 1294
Tth111I GACNNNGTC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 575
XagI CCTNNNNNAGG 1 cut(s) 1239
XapI RAATTY 4 cut(s) 508, 1107, 1475, 1653
XceI RCATGY 1 cut(s) 1194
XhoI CTCGAG 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 772
XmnI GAANNNNTTC 2 cut(s) 692, 1047
XspI CTAG 1 cut(s) 1302
Zsp2I ATGCAT 1 cut(s) 1342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.