Rh5DG421400

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018611
Physical Location & Seq
Forward (+)
67237749 .. 67239443
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG421400.1

Sequence Viewer

Length: 1383 bp
ATGTCCAATTCACAATCTGCAGCATGGCTGAACAGATATCATGAGGTTAATGAGGTACAGGTTGAGATAAGTTTACCGGTGAGAACTGCATATGGTTTATCATCTAGATCTCCTGGAAATATTCCAAATTGGTTCAGCAATAGAAGCAGCAACTTTTCTATAACAATCCCGCTTTCAAGGGACTTAACTAATAAGAGTAGGCTATGGATGGGATGTGCTTTCTATGTTGGTTTGGAAGTTGATGAGAATTTTGATACCAGTTCTGATTTCAAAGTCAGGCACAAGTTTGACTGTTGTTTTGATACCATGGAAGGTCCTGGTTACAAACAAACTCTATACCACGAGGTAAATGTTAAGGGTGTCCATTTCACTGGGTCAGTTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGTTGAATGCCTTCAATCAGTGCGGTAGGATCGAGGCAAACACTACAATTGATAGCCCATGTGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTCTACAAAAGAACAGATTTTATAGAGTTTGTCCATGCGATAAAAAGGACGACTGGGCTTTCAAGAATTAGGCAGAATAACAGTTTCTCTGGAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGGATTTCGTTGAAAGAACATCTTAGCTCGCTGCCTTCTGATTTAGAATGCTATGAGGGGCAATCCGGGGTGGGATTCGAGTACCATATTAGAGGCAAAGTCTTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGAAATATTCTGGTCAGCACTGGGTGTTTTATAGAGGTACTTTTCCTCAATATGGAATTCCAGAATGGTTCTGCAGTGTGAATGGTTCCTCGGCAGCCATCCCGCTAAATCCTATGTATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAGTTTCGATCCTGAAAAGACTTGCATACGCTGTTTTTTGGAAATTGGCTATCAGTGTGCCACAATAAGTGCATGCGTCACTAAATCTCTTATTAGTTTGAAGGTTCGTGGTTTCGTGGGTATGTTTTATGCATCACGTGAGTCACTTTCACATCTTATGTCAAATGATTACACTTCCATGATCAAAGCCAAGTTTTATAGTAACAGCAAGAACATAACGGTGCAGAGTTGTGGGTATCAAATTGTATTCCATGAAAATATGGAAGAATTTATGGAAACAATGCTGCAATGCTCAATCACATCTCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGAGAACTACCTCTACCCAAATAGAAAAGCAGAAAAGGAAGTCTTCCTGCGGATCGATCTGTTGCTTCCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

460

Amino Acids

52.26

Weight (kDa)

7.51

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 503
AccI GTMKAC 1 cut(s) 511
AciI CCGC 5 cut(s) 170, 401, 444, 890, 1357
AclWI GGATC 3 cut(s) 458, 953, 1367
AcsI RAATTY 3 cut(s) 247, 843, 1217
AcuI CTGAAG 1 cut(s) 770
AcvI CACGTG 1 cut(s) 1088
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 3 cut(s) 57, 728, 826
AfiI CCNNNNNNNGG 3 cut(s) 484, 839, 905
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 7 cut(s) 177, 271, 427, 436, 576, 658, 1051
AjnI CCWGG 2 cut(s) 112, 316
AluBI AGCT 3 cut(s) 630, 672, 923
AluI AGCT 3 cut(s) 630, 672, 923
Alw26I GTCTC 1 cut(s) 771
AlwI GGATC 3 cut(s) 458, 953, 1367
Ama87I CYCGRG 1 cut(s) 643
ApeKI GCWGC 7 cut(s) 20, 147, 630, 676, 881, 923, 1234
ApoI RAATTY 3 cut(s) 247, 843, 1217
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AsiGI ACCGGT 1 cut(s) 76
Asp700I GAANNNNTTC 2 cut(s) 431, 783
AspS9I GGNCC 1 cut(s) 314
AsuC2I CCSGG 1 cut(s) 712
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 508
BanI GGYRCC 1 cut(s) 503
BauI CACGAG 1 cut(s) 341
BbrPI CACGTG 1 cut(s) 1088
BbsI GAAGAC 1 cut(s) 1342
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 7 cut(s) 32, 159, 617, 663, 893, 910, 1221
BccI CCATC 3 cut(s) 202, 617, 893
BceAI ACGGC 1 cut(s) 917
BciT130I CCWGG 2 cut(s) 114, 318
BclI TGATCA 1 cut(s) 1131
BcnI CCSGG 1 cut(s) 712
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 18, 859
BglII AGATCT 1 cut(s) 107
BisI GCNGC 7 cut(s) 21, 148, 631, 677, 882, 924, 1235
BlsI GCNGC 7 cut(s) 22, 149, 632, 678, 883, 925, 1236
Bme1390I CCNGG 3 cut(s) 114, 318, 712
Bme18I GGWCC 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 2 cut(s) 505, 874
BmrFI CCNGG 3 cut(s) 114, 318, 712
BmrI ACTGGG 3 cut(s) 381, 576, 817
BmsI GCATC 2 cut(s) 412, 1091
BmuI ACTGGG 3 cut(s) 381, 576, 817
BpiI GAAGAC 1 cut(s) 1342
Bpu10I CCTNAGC 1 cut(s) 618
BpuMI CCSGG 1 cut(s) 712
Bsa29I ATCGAT 1 cut(s) 1362
BsaAI YACGTR 1 cut(s) 1088
BsaJI CCNNGG 3 cut(s) 306, 711, 876
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 3 cut(s) 484, 839, 905
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 4 cut(s) 258, 376, 571, 812
Bse3DI GCAATG 1 cut(s) 1244
BseBI CCWGG 2 cut(s) 114, 318
BseCI ATCGAT 1 cut(s) 1362
BseDI CCNNGG 3 cut(s) 306, 711, 876
BseGI GGATG 5 cut(s) 213, 218, 628, 751, 885
BseLI CCNNNNNNNGG 3 cut(s) 484, 839, 905
BseMI GCAATG 1 cut(s) 1244
BseMII CTCAG 3 cut(s) 609, 1268, 1277
BseNI ACTGG 4 cut(s) 258, 376, 571, 812
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 7 cut(s) 32, 159, 617, 663, 893, 910, 1221
BsgI GTGCAG 1 cut(s) 1193
BshNI GGYRCC 1 cut(s) 503
BshTI ACCGGT 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 1362
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 2 cut(s) 77, 711
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 3 cut(s) 484, 839, 905
BsmAI GTCTC 1 cut(s) 771
BsmBI CGTCTC 1 cut(s) 771
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 2 cut(s) 433, 698
BsoBI CYCGRG 1 cut(s) 643
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 5 cut(s) 170, 401, 444, 890, 1357
BspCNI CTCAG 3 cut(s) 610, 1267, 1276
BspDI ATCGAT 1 cut(s) 1362
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 505, 874
BspMAI CTGCAG 2 cut(s) 22, 863
BspPI GGATC 3 cut(s) 458, 953, 1367
BspT107I GGYRCC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 1244
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 4 cut(s) 258, 376, 571, 812
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 306, 711, 876
BssMI GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 2 cut(s) 114, 318
Bst4CI ACNGT 5 cut(s) 293, 596, 943, 953, 1171
BstBAI YACGTR 1 cut(s) 1088
BstC8I GCNNGC 2 cut(s) 674, 1024
BstDEI CTNAG 4 cut(s) 618, 668, 1254, 1263
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 5 cut(s) 213, 218, 628, 751, 885
BstKTI GATC 6 cut(s) 110, 453, 961, 1134, 1362, 1366
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
BstMWI GCNNNNNNNGC 2 cut(s) 144, 929
BstNI CCWGG 2 cut(s) 114, 318
BstNSI RCATGY 2 cut(s) 930, 1026
BstSCI CCNGG 3 cut(s) 112, 316, 710
BstSFI CTRYAG 2 cut(s) 18, 859
BstSLI GKGCMC 1 cut(s) 508
BstV1I GCAGC 7 cut(s) 32, 159, 617, 663, 893, 910, 1221
BstV2I GAAGAC 1 cut(s) 1342
BstX2I RGATCY 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 1 cut(s) 107
Bsu15I ATCGAT 1 cut(s) 1362
BsuTUI ATCGAT 1 cut(s) 1362
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 5 cut(s) 213, 218, 628, 751, 885
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 5 cut(s) 369, 446, 805, 868, 1010
Cac8I GCNNGC 2 cut(s) 674, 1024
CciI TCATGA 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 314
ClaI ATCGAT 1 cut(s) 1362
CseI GACGC 1 cut(s) 1015
Csp6I GTAC 3 cut(s) 56, 727, 825
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 9 cut(s) 24, 41, 307, 480, 548, 927, 1023, 1129, 1202
CviQI GTAC 3 cut(s) 56, 727, 825
DdeI CTNAG 4 cut(s) 618, 668, 1254, 1263
DpnI GATC 6 cut(s) 109, 452, 960, 1133, 1361, 1365
DpnII GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
DraIII CACNNNGTG 1 cut(s) 811
Eco130I CCWWGG 1 cut(s) 306
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 770
Eco72I CACGTG 1 cut(s) 1088
Eco88I CYCGRG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 2 cut(s) 112, 316
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 1084
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 771
FaeI CATG 9 cut(s) 27, 44, 310, 483, 551, 930, 1026, 1132, 1205
FalI AAGNNNNNCTT 4 cut(s) 651, 683, 1334, 1366
FaqI GGGAC 1 cut(s) 194
FatI CATG 9 cut(s) 23, 40, 306, 479, 547, 926, 1022, 1128, 1201
FauI CCCGC 2 cut(s) 177, 897
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 1 cut(s) 1131
FblI GTMKAC 1 cut(s) 511
Fnu4HI GCNGC 7 cut(s) 21, 148, 631, 677, 882, 924, 1235
FokI GGATG 5 cut(s) 220, 225, 635, 738, 872
Fsp4HI GCNGC 7 cut(s) 21, 148, 631, 677, 882, 924, 1235
FspBI CTAG 1 cut(s) 105
GluI GCNGC 7 cut(s) 21, 148, 631, 677, 882, 924, 1235
HapII CCGG 2 cut(s) 77, 711
HgaI GACGC 1 cut(s) 1015
Hin1II CATG 9 cut(s) 27, 44, 310, 483, 551, 930, 1026, 1132, 1205
HinfI GANTC 3 cut(s) 641, 720, 1091
HpaII CCGG 2 cut(s) 77, 711
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 74, 512
Hpy188I TCNGA 4 cut(s) 265, 685, 789, 1257
Hpy188III TCNNGA 6 cut(s) 41, 105, 576, 603, 848, 962
Hpy8I GTNNAC 2 cut(s) 74, 512
HpyAV CCTTC 5 cut(s) 305, 442, 690, 794, 1045
HpyCH4III ACNGT 5 cut(s) 293, 596, 943, 953, 1171
HpyCH4IV ACGT 1 cut(s) 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 929
HpyF3I CTNAG 4 cut(s) 618, 668, 1254, 1263
HpySE526I ACGT 1 cut(s) 1087
Hsp92II CATG 9 cut(s) 27, 44, 310, 483, 551, 930, 1026, 1132, 1205
Ksp22I TGATCA 1 cut(s) 1131
Kzo9I GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
Lsp1109I GCAGC 7 cut(s) 32, 159, 617, 663, 893, 910, 1221
LweI GCATC 2 cut(s) 412, 1091
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 1087
MaeIII GTNAC 4 cut(s) 320, 1027, 1092, 1151
MalI GATC 6 cut(s) 109, 452, 960, 1133, 1361, 1365
MboI GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
MboII GAAGA 3 cut(s) 1226, 1301, 1342
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 1 cut(s) 508
MlyI GAGTC 2 cut(s) 635, 1100
MmeI TCCRAC 1 cut(s) 361
Mph1103I ATGCAT 1 cut(s) 1084
MroXI GAANNNNTTC 2 cut(s) 431, 783
MseI TTAA 4 cut(s) 48, 185, 354, 492
MslI CAYNNNNRTG 2 cut(s) 1127, 1169
MspI CCGG 2 cut(s) 77, 711
MspR9I CCNGG 3 cut(s) 114, 318, 712
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 2 cut(s) 433, 698
MvaI CCWGG 2 cut(s) 114, 318
MwoI GCNNNNNNNGC 2 cut(s) 144, 929
NciI CCSGG 1 cut(s) 712
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 1 cut(s) 91
NdeII GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
NlaIII CATG 9 cut(s) 27, 44, 310, 483, 551, 930, 1026, 1132, 1205
NlaIV GGNNCC 2 cut(s) 505, 874
NmeAIII GCCGAG 1 cut(s) 857
NmuCI GTSAC 2 cut(s) 1027, 1092
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 2 cut(s) 930, 1026
PaeI GCATGC 1 cut(s) 1026
PaeR7I CTCGAG 1 cut(s) 643
PagI TCATGA 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 650
PctI GAATGC 2 cut(s) 433, 698
PdmI GAANNNNTTC 2 cut(s) 431, 783
PfeI GAWTC 1 cut(s) 720
PflFI GACNNNGTC 1 cut(s) 763
PfoI TCCNGGA 1 cut(s) 112
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 7 cut(s) 22, 149, 632, 678, 883, 925, 1236
PleI GAGTC 2 cut(s) 635, 1099
PmaCI CACGTG 1 cut(s) 1088
PmlI CACGTG 1 cut(s) 1088
PpsI GAGTC 2 cut(s) 635, 1099
Ppu21I YACGTR 1 cut(s) 1088
PpuMI RGGWCCY 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 2 cut(s) 112, 316
PspCI CACGTG 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 112, 316
PspN4I GGNNCC 2 cut(s) 505, 874
PspPI GGNCC 1 cut(s) 314
PspPPI RGGWCCY 1 cut(s) 314
PspXI VCTCGAGB 1 cut(s) 643
PsrI GAACNNNNNNTAC 2 cut(s) 1304, 1336
PstI CTGCAG 2 cut(s) 22, 863
PsuI RGATCY 1 cut(s) 107
PsyI GACNNNGTC 1 cut(s) 763
RsaI GTAC 3 cut(s) 57, 728, 826
RsaNI GTAC 3 cut(s) 56, 727, 825
RseI CAYNNNNRTG 2 cut(s) 1127, 1169
SaqAI TTAA 4 cut(s) 48, 185, 354, 492
SatI GCNGC 7 cut(s) 21, 148, 631, 677, 882, 924, 1235
Sau3AI GATC 6 cut(s) 107, 450, 958, 1131, 1359, 1363
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 2 cut(s) 635, 1100
ScrFI CCNGG 3 cut(s) 114, 318, 712
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 2 cut(s) 412, 1091
SfcI CTRYAG 2 cut(s) 18, 859
Sfr274I CTCGAG 1 cut(s) 643
SinI GGWCC 1 cut(s) 314
SlaI CTCGAG 1 cut(s) 643
SmiMI CAYNNNNRTG 2 cut(s) 1127, 1169
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
SphI GCATGC 1 cut(s) 1026
SsiI CCGC 5 cut(s) 170, 401, 444, 890, 1357
SspI AATATT 2 cut(s) 121, 794
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 112, 316, 710
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 5 cut(s) 293, 596, 943, 953, 1171
TaiI ACGT 1 cut(s) 1090
TaqI TCGA 5 cut(s) 453, 644, 723, 957, 1362
TfiI GAWTC 1 cut(s) 720
Tru1I TTAA 4 cut(s) 48, 185, 354, 492
Tru9I TTAA 4 cut(s) 48, 185, 354, 492
TscAI CASTG 5 cut(s) 376, 446, 812, 868, 1010
TseFI GTSAC 2 cut(s) 1027, 1092
TseI GCWGC 7 cut(s) 20, 147, 630, 676, 881, 923, 1234
Tsp45I GTSAC 2 cut(s) 1027, 1092
TspDTI ATGAA 2 cut(s) 740, 1218
TspRI CASTG 5 cut(s) 376, 446, 812, 868, 1010
Tth111I GACNNNGTC 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 3 cut(s) 247, 843, 1217
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 2 cut(s) 930, 1026
XhoI CTCGAG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 511
XmnI GAANNNNTTC 2 cut(s) 431, 783
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.