Rh5DG421100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018611
Physical Location & Seq
Forward (+)
67068281 .. 67140288
72008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG421100.1

Sequence Viewer

Length: 708 bp
ATGGACATAACTAATAAGAGTAGGCTATGGATGGGGTGTGCTTTCTATGTTGTTTTGGAAGTTGATGAGAATTTTGATACGGGTTCTGATTTCAAAGTCAGGCACAAGTTTGACTGTTGTTTTGATACCATGGAAGGTCCTGGTTACAAACAAACTCTATACCACGAGGTAAATGCTAAAGGTGTCCATTTGACTGGGTCAATTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGTTGAATGCCTTCAATCAGTGCAGTAGCATCGAGGCTAACACTACAATTGATAGCCCATGTGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTCTACGAAAAAAAAGATTTTAGAGGGTTTGTCCAAGCGATAAAAAGGGTGATTCGGGTTCCAAGAATTAGGCAGAATAACAGTTTCTCTGGAAGTCTATTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGCATTTCGTTGAAAGAACTTCTTAGCTCGCTGCCTTCAGATTTAGAATGCTATGAGGGGCAATGCGAGGGGGGATTCGAGTACCATATTGGAGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGGTCTCTCTCTCTCTCTCACACGCACACGCAGGCACGCCCACGTATGGGGTCATGGTCATGGTCAGAAATCTAATTCCAATTTTTTTTTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

235

Amino Acids

26.37

Weight (kDa)

6.23

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 334
AciI CCGC 1 cut(s) 224
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 2 cut(s) 489, 593
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 3 cut(s) 307, 656, 657
AgsI TTSAA 4 cut(s) 94, 250, 259, 481
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 2 cut(s) 453, 495
AluI AGCT 2 cut(s) 453, 495
Alw26I GTCTC 2 cut(s) 594, 619
Ama87I CYCGRG 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 453, 499
ApoI RAATTY 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 2 cut(s) 254, 606
AspS9I GGNCC 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 466
AvaII GGWCC 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 326
BauI CACGAG 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 440, 486
BccI CCATC 2 cut(s) 25, 440
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 256, 290
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 594, 619
BisI GCNGC 2 cut(s) 454, 500
BlsI GCNGC 2 cut(s) 455, 501
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 2 cut(s) 328, 396
BmrFI CCNGG 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 2 cut(s) 235, 282
BmuI ACTGGG 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 441
BsaAI YACGTR 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 1 cut(s) 129
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 656, 657
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 36, 451, 574
BseLI CCNNNNNNNGG 3 cut(s) 307, 656, 657
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 2 cut(s) 440, 486
BsgI GTGCAG 1 cut(s) 286
BshNI GGYRCC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 466
BslI CCNNNNNNNGG 3 cut(s) 307, 656, 657
BsmAI GTCTC 2 cut(s) 594, 619
BsmBI CGTCTC 1 cut(s) 594
BsmI GAATGC 2 cut(s) 256, 521
Bso31I GGTCTC 1 cut(s) 619
BsoBI CYCGRG 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 331
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 328, 396
BspT107I GGYRCC 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 536
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 129
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 116, 419
BstBAI YACGTR 1 cut(s) 654
BstC8I GCNNGC 3 cut(s) 497, 643, 647
BstDEI CTNAG 2 cut(s) 441, 491
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 36, 451, 574
BstMAI GTCTC 2 cut(s) 594, 619
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 440, 486
BstXI CCANNNNNNTGG 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 36, 451, 574
BtsIMutI CAGTG 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 497, 643, 647
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 550
CviAII CATG 4 cut(s) 130, 303, 664, 670
CviJI RGCY 6 cut(s) 25, 281, 300, 440, 453, 495
CviKI_1 RGCY 6 cut(s) 25, 281, 300, 440, 453, 495
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 2 cut(s) 441, 491
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 489, 593
Eco88I CYCGRG 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 4 cut(s) 133, 306, 667, 673
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FatI CATG 4 cut(s) 129, 302, 663, 669
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 454, 500
FokI GGATG 3 cut(s) 43, 458, 561
Fsp4HI GCNGC 2 cut(s) 454, 500
GluI GCNGC 2 cut(s) 454, 500
Hin1II CATG 4 cut(s) 133, 306, 667, 673
HinfI GANTC 3 cut(s) 388, 464, 543
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 3 cut(s) 88, 508, 677
Hpy188III TCNNGA 1 cut(s) 426
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 4 cut(s) 128, 265, 513, 617
HpyCH4III ACNGT 2 cut(s) 116, 419
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 2 cut(s) 267, 456
HpyF3I CTNAG 2 cut(s) 441, 491
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 4 cut(s) 133, 306, 667, 673
LpnPI CCDG 8 cut(s) 85, 126, 153, 180, 411, 593, 596, 627
Lsp1109I GCAGC 2 cut(s) 440, 486
LweI GCATC 2 cut(s) 235, 282
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 1 cut(s) 143
MfeI CAATTG 2 cut(s) 201, 291
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 7 cut(s) 70, 201, 291, 402, 459, 684, 690
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 9 cut(s) 160, 271, 304, 353, 436, 517, 529, 554, 595
MroXI GAANNNNTTC 2 cut(s) 254, 606
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 141
MunI CAATTG 2 cut(s) 201, 291
Mva1269I GAATGC 2 cut(s) 256, 521
MvaI CCWGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 129
NlaIII CATG 4 cut(s) 133, 306, 667, 673
NlaIV GGNNCC 2 cut(s) 328, 396
PaeR7I CTCGAG 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PctI GAATGC 2 cut(s) 256, 521
PdmI GAANNNNTTC 2 cut(s) 254, 606
PfeI GAWTC 2 cut(s) 388, 543
PflFI GACNNNGTC 2 cut(s) 196, 586
PkrI GCNGC 2 cut(s) 455, 501
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Ppu21I YACGTR 1 cut(s) 654
PpuMI RGGWCCY 1 cut(s) 137
Psp5II RGGWCCY 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 328, 396
PspPI GGNCC 1 cut(s) 137
PspPPI RGGWCCY 1 cut(s) 137
PspXI VCTCGAGB 1 cut(s) 466
PsyI GACNNNGTC 2 cut(s) 196, 586
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 2 cut(s) 454, 500
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 2 cut(s) 235, 282
Sfr274I CTCGAG 1 cut(s) 466
SinI GGWCC 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 466
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 7 cut(s) 70, 201, 291, 402, 459, 684, 690
SsiI CCGC 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 116, 419
TaiI ACGT 1 cut(s) 656
TaqI TCGA 3 cut(s) 276, 467, 546
TasI AATT 7 cut(s) 70, 201, 291, 402, 459, 684, 690
TfiI GAWTC 2 cut(s) 388, 543
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 269
TseI GCWGC 2 cut(s) 453, 499
TspDTI ATGAA 1 cut(s) 563
TspRI CASTG 1 cut(s) 269
Tth111I GACNNNGTC 2 cut(s) 196, 586
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 334
XmnI GAANNNNTTC 2 cut(s) 254, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.