Rh5BG406500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018611
Physical Location & Seq
Forward (+)
65889510 .. 65890678
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG406500.1

Sequence Viewer

Length: 948 bp
ATGAGTGGTGCCCGTCTACTGTACAAAAGAGCAGATTTTATAGGGTTTATCCGAGCAATAAAATCGATGAATAAACTATCAAGAATTATGCAGAATAACAGTTCCTCTATAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGCATTTTGTTGAAAGAACATCTTAGCTCACTGCCTTCAGATTTAAAATGCTATGAGGGGCACGCCGGCTATGCGGGAAGAAATTACTACGTTATTAAAGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGAAATATTCTGGTCAGCACTCGGTGTTTTATACAGAAATTTTTCCTCAATATGGAATTCCAGAATGGTTCTTCAGTGTGAATGGTTCCTCCGCAGCCTTCCCGCTAAATCCTATGAATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAATTTGGATCCTGAAAAGCCTGACATAGGCTTTGAGTTGAAAATTGGCGGTCGGAATATCAGTGGACCCATCACTGAATCCCTTACTAGTTTGAAGGTTGGTGGTTTCATGGGTATGTTTTATGGATCACGTGGGTCACTTTCACATCTTATGTCATCTGATTGCACTTCCACCATCAAAGTCAGGTTTTATAGTAAGAGCAAGAACATAACGGTGCAGAGTTGTGGGTATCAAATTGTATTCCATGAAAATATGGAAGAATTTATGGAAACAATACTGCAATGTTCAGTCGCATCGCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAACCACCGAAAGAAAAGGGGAACTCTCTCTACCCAAATAGAAAAGGAGAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGAGAACTCTCTCTACCCGACTTTTGAAGTCTCCCTGTAGATCGATCTGTTGCTTCCTGTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

315

Amino Acids

35.16

Weight (kDa)

9.35

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 16
AciI CCGC 4 cut(s) 224, 390, 401, 510
AclWI GGATC 3 cut(s) 464, 477, 595
AcsI RAATTY 3 cut(s) 336, 354, 722
AcuI CTGAAG 3 cut(s) 171, 281, 355
AcvI CACGTG 1 cut(s) 593
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 3 cut(s) 350, 416, 488
AgsI TTSAA 4 cut(s) 163, 502, 556, 907
AhlI ACTAGT 1 cut(s) 548
AluBI AGCT 3 cut(s) 135, 177, 434
AluI AGCT 3 cut(s) 135, 177, 434
Alw26I GTCTC 2 cut(s) 282, 915
AlwI GGATC 3 cut(s) 464, 477, 595
Ama87I CYCGRG 1 cut(s) 148
ApeKI GCWGC 3 cut(s) 135, 392, 434
ApoI RAATTY 3 cut(s) 336, 354, 722
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp700I GAANNNNTTC 2 cut(s) 294, 339
AspS9I GGNCC 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 527
BaeGI GKGCMC 2 cut(s) 13, 213
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 8
BbrPI CACGTG 1 cut(s) 593
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 122, 404, 421
BccI CCATC 3 cut(s) 122, 539, 644
BceAI ACGGC 1 cut(s) 428
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BcoDI GTCTC 2 cut(s) 282, 915
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 916
BisI GCNGC 3 cut(s) 136, 393, 435
BlsI GCNGC 3 cut(s) 137, 394, 436
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 4 cut(s) 10, 385, 471, 529
BmsI GCATC 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 123
Bsa29I ATCGAT 2 cut(s) 65, 923
BsaAI YACGTR 1 cut(s) 593
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 416, 488
Bse118I RCCGGY 1 cut(s) 215
Bse3DI GCAATG 1 cut(s) 749
BseCI ATCGAT 2 cut(s) 65, 923
BseGI GGATG 2 cut(s) 133, 262
BseLI CCNNNNNNNGG 3 cut(s) 350, 416, 488
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 2 cut(s) 114, 782
BseSI GKGCMC 2 cut(s) 13, 213
BseXI GCAGC 3 cut(s) 122, 404, 421
BsgI GTGCAG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 514
BshNI GGYRCC 1 cut(s) 8
BshVI ATCGAT 2 cut(s) 65, 923
BsiEI CGRYCG 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 3 cut(s) 350, 416, 488
BsmAI GTCTC 2 cut(s) 282, 915
BsmBI CGTCTC 1 cut(s) 282
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 13, 213
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 4 cut(s) 469, 587, 920, 924
BspACI CCGC 4 cut(s) 224, 390, 401, 510
BspCNI CTCAG 2 cut(s) 115, 781
BspDI ATCGAT 2 cut(s) 65, 923
BspLI GGNNCC 4 cut(s) 10, 385, 471, 529
BspPI GGATC 3 cut(s) 464, 477, 595
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 21
BssAI RCCGGY 1 cut(s) 215
BssMI GATC 4 cut(s) 469, 587, 920, 924
Bst4CI ACNGT 4 cut(s) 21, 101, 454, 676
BstAUI TGTACA 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 213, 217
BstDEI CTNAG 3 cut(s) 123, 173, 768
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 133, 262
BstKTI GATC 4 cut(s) 472, 590, 923, 927
BstMAI GTCTC 2 cut(s) 282, 915
BstMBI GATC 4 cut(s) 469, 587, 920, 924
BstMCI CGRYCG 1 cut(s) 514
BstMWI GCNNNNNNNGC 2 cut(s) 221, 440
BstNSI RCATGY 1 cut(s) 441
BstSFI CTRYAG 1 cut(s) 916
BstSLI GKGCMC 2 cut(s) 13, 213
BstV1I GCAGC 3 cut(s) 122, 404, 421
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
Bsu15I ATCGAT 2 cut(s) 65, 923
BsuTUI ATCGAT 2 cut(s) 65, 923
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 2 cut(s) 133, 262
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 4 cut(s) 179, 379, 529, 534
Cac8I GCNNGC 2 cut(s) 213, 217
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 527
ClaI ATCGAT 2 cut(s) 65, 923
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 438, 571, 707
CviJI RGCY 8 cut(s) 122, 135, 177, 219, 395, 434, 481, 492
CviKI_1 RGCY 8 cut(s) 122, 135, 177, 219, 395, 434, 481, 492
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 3 cut(s) 123, 173, 768
DpnI GATC 4 cut(s) 471, 589, 922, 926
DpnII GATC 4 cut(s) 469, 587, 920, 924
DraI TTTAAA 1 cut(s) 195
DraIII CACNNNGTG 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 3 cut(s) 171, 281, 355
Eco72I CACGTG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 354
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 3 cut(s) 441, 574, 710
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 3 cut(s) 437, 570, 706
FauI CCCGC 2 cut(s) 217, 408
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 136, 393, 435
FokI GGATG 2 cut(s) 140, 249
Fsp4HI GCNGC 3 cut(s) 136, 393, 435
FspBI CTAG 1 cut(s) 549
GluI GCNGC 3 cut(s) 136, 393, 435
HapII CCGG 1 cut(s) 216
Hin1II CATG 3 cut(s) 441, 574, 710
HinfI GANTC 2 cut(s) 146, 539
HpaII CCGG 1 cut(s) 216
Hpy166II GTNNAC 2 cut(s) 17, 527
Hpy188I TCNGA 5 cut(s) 53, 190, 300, 516, 622
Hpy188III TCNNGA 3 cut(s) 81, 359, 473
Hpy8I GTNNAC 2 cut(s) 17, 527
HpyAV CCTTC 4 cut(s) 195, 305, 406, 550
HpyCH4III ACNGT 4 cut(s) 21, 101, 454, 676
HpyCH4IV ACGT 2 cut(s) 240, 592
HpyCH4V TGCA 6 cut(s) 91, 138, 437, 627, 679, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 440
HpyF3I CTNAG 3 cut(s) 123, 173, 768
HpySE526I ACGT 2 cut(s) 240, 592
Hsp92II CATG 3 cut(s) 441, 574, 710
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 4 cut(s) 469, 587, 920, 924
LpnPI CCDG 8 cut(s) 229, 281, 294, 372, 486, 495, 631, 928
Lsp1109I GCAGC 3 cut(s) 122, 404, 421
LweI GCATC 1 cut(s) 764
MaeI CTAG 1 cut(s) 549
MaeII ACGT 2 cut(s) 240, 592
MaeIII GTNAC 1 cut(s) 597
MalI GATC 4 cut(s) 471, 589, 922, 926
MboI GATC 4 cut(s) 469, 587, 920, 924
MboII GAAGA 5 cut(s) 240, 361, 731, 806, 874
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 13, 213
MluCI AATT 9 cut(s) 84, 141, 232, 336, 354, 463, 504, 696, 722
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 6 cut(s) 115, 118, 199, 283, 354, 397
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 2 cut(s) 294, 339
MseI TTAA 2 cut(s) 194, 246
MslI CAYNNNNRTG 2 cut(s) 575, 674
MspI CCGG 1 cut(s) 216
MwoI GCNNNNNNNGC 2 cut(s) 221, 440
NaeI GCCGGC 1 cut(s) 217
NdeII GATC 4 cut(s) 469, 587, 920, 924
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 3 cut(s) 441, 574, 710
NlaIV GGNNCC 4 cut(s) 10, 385, 471, 529
NmuCI GTSAC 1 cut(s) 597
NspI RCATGY 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 2 cut(s) 294, 339
PfeI GAWTC 1 cut(s) 539
PflFI GACNNNGTC 1 cut(s) 274
PkrI GCNGC 3 cut(s) 137, 394, 436
PleI GAGTC 1 cut(s) 140
PmaCI CACGTG 1 cut(s) 593
PmlI CACGTG 1 cut(s) 593
PpsI GAGTC 1 cut(s) 140
Ppu21I YACGTR 1 cut(s) 593
PspCI CACGTG 1 cut(s) 593
PspN4I GGNNCC 4 cut(s) 10, 385, 471, 529
PspPI GGNCC 1 cut(s) 527
PspXI VCTCGAGB 1 cut(s) 148
PsrI GAACNNNNNNTAC 4 cut(s) 809, 841, 877, 909
PsuI RGATCY 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 274
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 2 cut(s) 575, 674
SaqAI TTAA 2 cut(s) 194, 246
SatI GCNGC 3 cut(s) 136, 393, 435
Sau3AI GATC 4 cut(s) 469, 587, 920, 924
Sau96I GGNCC 1 cut(s) 527
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 2 cut(s) 13, 213
SetI ASST 8 cut(s) 137, 179, 243, 275, 436, 561, 595, 650
SfaNI GCATC 1 cut(s) 764
SfcI CTRYAG 1 cut(s) 916
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 575, 674
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 9 cut(s) 84, 141, 232, 336, 354, 463, 504, 696, 722
SsiI CCGC 4 cut(s) 224, 390, 401, 510
SspI AATATT 1 cut(s) 305
SspMI CTAG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 21, 101, 454, 676
TaiI ACGT 2 cut(s) 243, 595
TaqI TCGA 3 cut(s) 65, 149, 923
TasI AATT 9 cut(s) 84, 141, 232, 336, 354, 463, 504, 696, 722
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 2 cut(s) 194, 246
Tru9I TTAA 2 cut(s) 194, 246
TscAI CASTG 4 cut(s) 186, 379, 529, 541
TseFI GTSAC 1 cut(s) 597
TseI GCWGC 3 cut(s) 135, 392, 434
Tsp45I GTSAC 1 cut(s) 597
TspDTI ATGAA 5 cut(s) 83, 251, 428, 559, 723
TspRI CASTG 4 cut(s) 186, 379, 529, 541
Tth111I GACNNNGTC 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 527
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 3 cut(s) 336, 354, 722
XceI RCATGY 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 2 cut(s) 294, 339
XspI CTAG 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.