Rh5DG421600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018611
Physical Location & Seq
Forward (+)
67269058 .. 67270216
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG421600.1

Sequence Viewer

Length: 948 bp
ATGAGTGGTGCCCGTCTACTGTACAAAAGAACAGATTTTATAGAGTTTATCCAAGCAATACAATCGGCGACTCAACTATCAAGAATTATGCAGAATAACAGTTCCTGTATAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGCATTTTGTTGAAAGAACATCTTGGCTCGCTGCCTTCAGATTTAGAATGCTATGAGGGGCACGCCGCCTATGCGGGAAGAAATTACTACGTTATTAAAGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGAAATATTCTGGTCAGCACTCGGTGTTTTATAGAGAAACTTTTCCACAATATGGAATTCCAGAATGGTTCTGCAGTGTGAATAGTTCCTCGGCAGCCTTCCCGCTAAATCCTATGAATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAATTTGGATCCTGAAAAGCCTGACATAGGCTTTAATTTGAAAATTGGCTGTCGGAATATCAGTGGACACATCTCTGAATCCCGTACTAGTTTGAAGGTTGGTGGTTTCATGGGTATGCTTTATGCATCACGTGGGTCACTTTCACATCTTATGTCATCTGATTGCACTTCCACCATCAAAGTCAGGTTTTATAGTAACAGCAAGAACATAACGGTACAGAGTTGTGGGTATCAAATTGTATTCCATGAAAATATGGAAGAATTTATGGAAACAATACTGCAATGTTCAGTCGCATCTCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGGGAACTCTCTCTACCCAAATAGAAAAGGAGAAAAGATACCAGTAGAGAAGAAACACCCAAAGAAAATGAGAACTCTCTCTACCCGAGTTTTGAAGTCTCCCTGTAGATTGATCTGTTGCTTCCTGTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

315

Amino Acids

35.16

Weight (kDa)

8.88

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018611)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060391
rosa_roxburghii Rroxscaffold_1G00020430
rosa_samantha Rh5AG394100 Rh5AG394300 Rh5BG406500 Rh5CG398000 Rh5CG430300 Rh5DG421100 Rh5DG421400 Rh5DG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 350
AccI GTMKAC 1 cut(s) 16
AciI CCGC 3 cut(s) 216, 224, 401
AclWI GGATC 2 cut(s) 464, 477
AcsI RAATTY 2 cut(s) 354, 722
AcuI CTGAAG 2 cut(s) 171, 281
AcvI CACGTG 1 cut(s) 593
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 3 cut(s) 23, 547, 678
AfiI CCNNNNNNNGG 3 cut(s) 350, 416, 488
AgsI TTSAA 4 cut(s) 163, 502, 556, 907
AhlI ACTAGT 1 cut(s) 548
AluBI AGCT 2 cut(s) 135, 434
AluI AGCT 2 cut(s) 135, 434
Alw26I GTCTC 2 cut(s) 282, 915
AlwI GGATC 2 cut(s) 464, 477
AlwNI CAGNNNCTG 1 cut(s) 105
Ama87I CYCGRG 2 cut(s) 148, 897
ApeKI GCWGC 4 cut(s) 135, 181, 392, 434
ApoI RAATTY 2 cut(s) 354, 722
Asp700I GAANNNNTTC 2 cut(s) 294, 339
AvaI CYCGRG 2 cut(s) 148, 897
BaeGI GKGCMC 2 cut(s) 13, 213
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 8
BbrPI CACGTG 1 cut(s) 593
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 122, 168, 404, 421
BccI CCATC 2 cut(s) 122, 644
BceAI ACGGC 1 cut(s) 428
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BcoDI GTCTC 2 cut(s) 282, 915
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 2 cut(s) 370, 916
BisI GCNGC 5 cut(s) 136, 182, 216, 393, 435
BlsI GCNGC 5 cut(s) 137, 183, 217, 394, 436
BmeT110I CYCGRG 2 cut(s) 148, 897
BmiI GGNNCC 2 cut(s) 10, 471
BmsI GCATC 2 cut(s) 596, 764
Bpu10I CCTNAGC 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 593
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 416, 488
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 1 cut(s) 749
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 133, 262
BseLI CCNNNNNNNGG 3 cut(s) 350, 416, 488
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 3 cut(s) 114, 773, 782
BseNI ACTGG 1 cut(s) 854
BseSI GKGCMC 2 cut(s) 13, 213
BseXI GCAGC 4 cut(s) 122, 168, 404, 421
BshNI GGYRCC 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 148, 897
BslI CCNNNNNNNGG 3 cut(s) 350, 416, 488
BsmAI GTCTC 2 cut(s) 282, 915
BsmBI CGTCTC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 203
BsoBI CYCGRG 2 cut(s) 148, 897
Bsp1286I GDGCHC 2 cut(s) 13, 213
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 2 cut(s) 469, 924
BspACI CCGC 3 cut(s) 216, 224, 401
BspCNI CTCAG 3 cut(s) 115, 772, 781
BspLI GGNNCC 2 cut(s) 10, 471
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 2 cut(s) 464, 477
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 749
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 1 cut(s) 854
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 469, 924
Bst4CI ACNGT 4 cut(s) 21, 101, 454, 676
BstAUI TGTACA 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 179, 213
BstDEI CTNAG 3 cut(s) 123, 759, 768
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 133, 262
BstKTI GATC 2 cut(s) 472, 927
BstMAI GTCTC 2 cut(s) 282, 915
BstMBI GATC 2 cut(s) 469, 924
BstMWI GCNNNNNNNGC 2 cut(s) 221, 440
BstNSI RCATGY 1 cut(s) 441
BstSFI CTRYAG 2 cut(s) 370, 916
BstSLI GKGCMC 2 cut(s) 13, 213
BstV1I GCAGC 4 cut(s) 122, 168, 404, 421
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BtsCI GGATG 2 cut(s) 133, 262
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 379, 529
Cac8I GCNNGC 2 cut(s) 179, 213
CaiI CAGNNNCTG 1 cut(s) 105
Csp6I GTAC 3 cut(s) 22, 546, 677
CviAII CATG 3 cut(s) 438, 571, 707
CviJI RGCY 8 cut(s) 122, 135, 177, 395, 434, 481, 492, 510
CviKI_1 RGCY 8 cut(s) 122, 135, 177, 395, 434, 481, 492, 510
CviQI GTAC 3 cut(s) 22, 546, 677
DdeI CTNAG 3 cut(s) 123, 759, 768
DpnI GATC 2 cut(s) 471, 926
DpnII GATC 2 cut(s) 469, 924
DraIII CACNNNGTG 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 171, 281
Eco72I CACGTG 1 cut(s) 593
Eco88I CYCGRG 2 cut(s) 148, 897
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 589
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 3 cut(s) 441, 574, 710
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 3 cut(s) 437, 570, 706
FauI CCCGC 2 cut(s) 217, 408
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 5 cut(s) 136, 182, 216, 393, 435
FokI GGATG 2 cut(s) 140, 249
Fsp4HI GCNGC 5 cut(s) 136, 182, 216, 393, 435
FspBI CTAG 1 cut(s) 549
GluI GCNGC 5 cut(s) 136, 182, 216, 393, 435
Hin1II CATG 3 cut(s) 441, 574, 710
HinfI GANTC 3 cut(s) 70, 146, 539
Hpy166II GTNNAC 2 cut(s) 17, 527
Hpy188I TCNGA 6 cut(s) 190, 300, 516, 538, 622, 762
Hpy188III TCNNGA 3 cut(s) 81, 359, 473
Hpy8I GTNNAC 2 cut(s) 17, 527
HpyAV CCTTC 4 cut(s) 195, 305, 406, 550
HpyCH4III ACNGT 4 cut(s) 21, 101, 454, 676
HpyCH4IV ACGT 2 cut(s) 240, 592
HpyCH4V TGCA 7 cut(s) 91, 138, 372, 437, 587, 627, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 440
HpyF3I CTNAG 3 cut(s) 123, 759, 768
HpySE526I ACGT 2 cut(s) 240, 592
Hsp92II CATG 3 cut(s) 441, 574, 710
Kzo9I GATC 2 cut(s) 469, 924
LpnPI CCDG 9 cut(s) 118, 281, 294, 372, 486, 495, 631, 867, 928
Lsp1109I GCAGC 4 cut(s) 122, 168, 404, 421
LweI GCATC 2 cut(s) 596, 764
MaeI CTAG 1 cut(s) 549
MaeII ACGT 2 cut(s) 240, 592
MaeIII GTNAC 2 cut(s) 597, 656
MalI GATC 2 cut(s) 471, 926
MboI GATC 2 cut(s) 469, 924
MboII GAAGA 4 cut(s) 240, 731, 806, 874
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 13, 213
MluCI AATT 9 cut(s) 84, 141, 232, 354, 463, 496, 504, 696, 722
MlyI GAGTC 2 cut(s) 64, 140
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 4 cut(s) 118, 199, 283, 397
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 294, 339
MseI TTAA 2 cut(s) 246, 495
MslI CAYNNNNRTG 1 cut(s) 575
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 221, 440
NdeII GATC 2 cut(s) 469, 924
NlaIII CATG 3 cut(s) 441, 574, 710
NlaIV GGNNCC 2 cut(s) 10, 471
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 597
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 148
PctI GAATGC 1 cut(s) 203
PdmI GAANNNNTTC 2 cut(s) 294, 339
PfeI GAWTC 1 cut(s) 539
PflFI GACNNNGTC 1 cut(s) 274
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 5 cut(s) 137, 183, 217, 394, 436
PleI GAGTC 2 cut(s) 64, 140
PmaCI CACGTG 1 cut(s) 593
PmlI CACGTG 1 cut(s) 593
PpsI GAGTC 2 cut(s) 64, 140
Ppu21I YACGTR 1 cut(s) 593
PspCI CACGTG 1 cut(s) 593
PspN4I GGNNCC 2 cut(s) 10, 471
PspXI VCTCGAGB 1 cut(s) 148
PsrI GAACNNNNNNTAC 4 cut(s) 809, 841, 877, 909
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 274
RsaI GTAC 3 cut(s) 23, 547, 678
RsaNI GTAC 3 cut(s) 22, 546, 677
RseI CAYNNNNRTG 1 cut(s) 575
SaqAI TTAA 2 cut(s) 246, 495
SatI GCNGC 5 cut(s) 136, 182, 216, 393, 435
Sau3AI GATC 2 cut(s) 469, 924
SchI GAGTC 2 cut(s) 64, 140
SduI GDGCHC 2 cut(s) 13, 213
SetI ASST 7 cut(s) 137, 243, 275, 436, 561, 595, 650
SfaNI GCATC 2 cut(s) 596, 764
SfcI CTRYAG 2 cut(s) 370, 916
Sfr274I CTCGAG 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 9 cut(s) 84, 141, 232, 354, 463, 496, 504, 696, 722
SsiI CCGC 3 cut(s) 216, 224, 401
SspI AATATT 1 cut(s) 305
SspMI CTAG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 21, 101, 454, 676
TaiI ACGT 2 cut(s) 243, 595
TaqI TCGA 1 cut(s) 149
TasI AATT 9 cut(s) 84, 141, 232, 354, 463, 496, 504, 696, 722
TatI WGTACW 1 cut(s) 21
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 2 cut(s) 246, 495
Tru9I TTAA 2 cut(s) 246, 495
TscAI CASTG 2 cut(s) 379, 529
TseFI GTSAC 1 cut(s) 597
TseI GCWGC 4 cut(s) 135, 181, 392, 434
Tsp45I GTSAC 1 cut(s) 597
TspDTI ATGAA 4 cut(s) 251, 428, 559, 723
TspRI CASTG 2 cut(s) 379, 529
Tth111I GACNNNGTC 1 cut(s) 274
Van91I CCANNNNNTGG 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 2 cut(s) 354, 722
XceI RCATGY 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 2 cut(s) 294, 339
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.