Rh5BG418700

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018508
Physical Location & Seq
Reverse (-)
67810277 .. 67811131
855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG418700.1

Sequence Viewer

Length: 516 bp
ATGGGTGTAGCTCTTTTTTCATTTGCTAGGCTCTTTGTTCTCTCTTTCATCACATTGGGTCTCATTCCTCAGCTAGCTCCTGCAAGCATAACAAGGCACTACAAATTTGATGTACAACTGCAAAATGTGACAAGGCTGTGCCATACCAAAAGCATTGTAACCATAAACGGTCAATTTCCTGGGCCTCGAATTGTTGCTAGGGAGGGTGATCGCCTCCTCATTAAAGTTGTTAACCATGTCCGAAGCAACATTTCCATCCATTGGCATGGAATTCGACAGCTTCGAACTGGGTGGGCTGATGGTCCAGCATACATTACCCAATGCCCAATACAAACCGGCAACAGCTATGTGTACAACTTCACCATTGTAGGCCAGAGGGGCACTCTTTGGTGGCATGCTCACATTTCATGGCTAAGATCAACCATCTATGGTCCCCTTATCATTCTTCCGGAGCGTGGTGTTCCTTATCCATTCACCAAACCCTATAAGGAAGTTCCCATCATCTTTGGTAAGTAA

Protein Analysis

171

Amino Acids

19.46

Weight (kDa)

10.33

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 151 1.2e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018508)

Species Orthologous Gene IDs
malus_domestica MD12G1156900.v1.1
pyrus_communis pycom11g10070
rosa_chinensis RchiOBHm_Chr6g0263741
rosa_multiflora Rmu_sc0024604.1_g000001
rosa_samantha Rh3BG132800 Rh5BG418700 Rh5BG419000 Rh5BG523200 Rh5DG535500 Rh6AG129000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 448
AcsI RAATTY 2 cut(s) 104, 270
AfaI GTAC 2 cut(s) 114, 353
AfiI CCNNNNNNNGG 2 cut(s) 261, 455
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 5 cut(s) 11, 73, 77, 280, 345
AluI AGCT 5 cut(s) 11, 73, 77, 280, 345
Alw26I GTCTC 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 182, 370
ApoI RAATTY 2 cut(s) 104, 270
AspS9I GGNCC 3 cut(s) 182, 302, 431
AsuHPI GGTGA 3 cut(s) 218, 352, 466
AsuII TTCGAA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 73
AvaII GGWCC 2 cut(s) 302, 431
BaeGI GKGCMC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 69
BccI CCATC 4 cut(s) 263, 293, 431, 506
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 3 cut(s) 27, 74, 198
BglI GCCNNNNNGGC 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 2 cut(s) 302, 431
BmgT120I GGNCC 3 cut(s) 182, 302, 431
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 180
BmrI ACTGGG 1 cut(s) 297
BmtI GCTAGC 1 cut(s) 77
BmuI ACTGGG 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 367, 399
Bpu10I CCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 455
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 1 cut(s) 292
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 2 cut(s) 261, 455
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 383
BshFI GGCC 2 cut(s) 184, 372
BsiSI CCGG 2 cut(s) 336, 449
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 261, 455
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 2 cut(s) 184, 372
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 283
Bsp1286I GDGCHC 1 cut(s) 383
Bsp13I TCCGGA 1 cut(s) 448
Bsp1407I TGTACA 2 cut(s) 112, 351
Bsp143I GATC 2 cut(s) 208, 416
BspANI GGCC 2 cut(s) 184, 372
BspCNI CTCAG 1 cut(s) 82
BspEI TCCGGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 433
BspOI GCTAGC 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 283
BspTNI GGTCTC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 335
BsrGI TGTACA 2 cut(s) 112, 351
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 208, 416
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 170
BstAUI TGTACA 2 cut(s) 112, 351
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 3 cut(s) 75, 85, 396
BstDEI CTNAG 2 cut(s) 69, 413
BstF5I GGATG 1 cut(s) 255
BstKTI GATC 2 cut(s) 211, 419
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 2 cut(s) 208, 416
BstMWI GCNNNNNNNGC 1 cut(s) 378
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 383
BstXI CCANNNNNNTGG 1 cut(s) 266
BsuRI GGCC 2 cut(s) 184, 372
BtsCI GGATG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 75, 85, 396
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 3 cut(s) 182, 302, 431
Csp6I GTAC 2 cut(s) 113, 352
CviAII CATG 4 cut(s) 236, 266, 395, 408
CviQI GTAC 2 cut(s) 113, 352
DdeI CTNAG 2 cut(s) 69, 413
DpnI GATC 2 cut(s) 210, 418
DpnII GATC 2 cut(s) 208, 416
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 302, 431
EcoRI GAATTC 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 178
FaeI CATG 4 cut(s) 239, 269, 398, 411
FaqI GGGAC 1 cut(s) 417
FatI CATG 4 cut(s) 235, 265, 394, 407
FokI GGATG 1 cut(s) 242
FspBI CTAG 3 cut(s) 27, 74, 198
HaeIII GGCC 2 cut(s) 184, 372
HapII CCGG 2 cut(s) 336, 449
Hin1II CATG 4 cut(s) 239, 269, 398, 411
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 2 cut(s) 336, 449
HphI GGTGA 3 cut(s) 218, 352, 466
Hpy166II GTNNAC 2 cut(s) 232, 352
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 1 cut(s) 449
Hpy8I GTNNAC 2 cut(s) 232, 352
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 2 cut(s) 83, 121
HpyF10VI GCNNNNNNNGC 1 cut(s) 378
HpyF3I CTNAG 2 cut(s) 69, 413
Hsp92II CATG 4 cut(s) 239, 269, 398, 411
Kpn2I TCCGGA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 208, 416
LmnI GCTCC 2 cut(s) 82, 451
LpnPI CCDG 8 cut(s) 93, 165, 192, 273, 318, 349, 386, 462
MaeI CTAG 3 cut(s) 27, 74, 198
MaeIII GTNAC 2 cut(s) 127, 157
MalI GATC 2 cut(s) 210, 418
MboI GATC 2 cut(s) 208, 416
MboII GAAGA 1 cut(s) 437
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 4 cut(s) 104, 173, 189, 270
MnlI CCTC 6 cut(s) 78, 195, 196, 224, 227, 369
MroI TCCGGA 1 cut(s) 448
MseI TTAA 2 cut(s) 222, 231
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 2 cut(s) 336, 449
MspR9I CCNGG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 1 cut(s) 378
NdeII GATC 2 cut(s) 208, 416
NheI GCTAGC 1 cut(s) 73
NlaIII CATG 4 cut(s) 239, 269, 398, 411
NlaIV GGNNCC 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 1 cut(s) 283
PaeI GCATGC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 3 cut(s) 182, 302, 431
RsaI GTAC 2 cut(s) 114, 353
RsaNI GTAC 2 cut(s) 113, 352
RseI CAYNNNNRTG 1 cut(s) 264
SaqAI TTAA 2 cut(s) 222, 231
Sau3AI GATC 2 cut(s) 208, 416
Sau96I GGNCC 3 cut(s) 182, 302, 431
ScrFI CCNGG 1 cut(s) 180
SduI GDGCHC 1 cut(s) 383
SetI ASST 5 cut(s) 13, 75, 79, 282, 347
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 2 cut(s) 302, 431
SmiMI CAYNNNNRTG 1 cut(s) 264
SphI GCATGC 1 cut(s) 398
Sse9I AATT 4 cut(s) 104, 173, 189, 270
SspMI CTAG 3 cut(s) 27, 74, 198
StyD4I CCNGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 3 cut(s) 187, 274, 283
TasI AATT 4 cut(s) 104, 173, 189, 270
TatI WGTACW 2 cut(s) 112, 351
Tru1I TTAA 2 cut(s) 222, 231
Tru9I TTAA 2 cut(s) 222, 231
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 9, 37, 396
Van91I CCANNNNNTGG 1 cut(s) 261
VpaK11BI GGWCC 2 cut(s) 302, 431
XapI RAATTY 2 cut(s) 104, 270
XceI RCATGY 1 cut(s) 398
XspI CTAG 3 cut(s) 27, 74, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.