Rh5DG129500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018248
Physical Location & Seq
Forward (+)
12466998 .. 12467555
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG129500.1

Sequence Viewer

Length: 558 bp
ATGGGTGAGATTGAAGGCGAGGACGTTGGGCATGTCATCGCTGAACTCCGTCTCCACGCCGCAGTAGCGAGACTCGCTTTTGTCACCTGCTCTTTCCCCAAGCGGCATTTTTGGTACTACTACTCTACGTCCCTGCTCGAACTTGCTCTCGGAGAAGCCCAAGGCGGCCTGCTGGAGGACGATGGCGGTGGTGGCGTTGTCGACGATGGAGACCTCGTCGACGTGGTCGGCGTTGATGAGGGTGCGGGAGTGGATGATGCCGGCTTGGAGGTGGTTGAAGTAGAAGTGATTGGGCTGGGAGAGGTACTTGAGCTGCCAGCGGCGCTGGGCCTGGAGGACGGAGTCAGGGCAGGACCCGAAGCTGCCGTTGTTGATTCGGGCGACGGTGGGGTCGTGGTGGGAGAATTCGGCGGCGATTTCGGAGGGGGTGATGAAGGAGAGTTTGGGCTTTTTGGAGGCGTGGGTGTTGGAATCGCCGTTCAGGGTGTGGTTGTGGTCGGGGGAGGAGGCCATTGCTGGGGGAGGAAGATTTTAGGGTTAGGGTTTGAAAGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

18.58

Weight (kDa)

4.05

Isoelectric Point (pI)

24.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
AasI GACNNNNNNGTC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 95
AccI GTMKAC 2 cut(s) 201, 219
AciI CCGC 7 cut(s) 60, 103, 165, 186, 245, 320, 411
AcsI RAATTY 1 cut(s) 404
AfaI GTAC 2 cut(s) 116, 306
AfiI CCNNNNNNNGG 2 cut(s) 175, 517
AgsI TTSAA 3 cut(s) 14, 278, 548
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 330
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AloI GAACNNNNNNTCC 4 cut(s) 36, 68, 462, 494
AluBI AGCT 2 cut(s) 313, 362
AluI AGCT 2 cut(s) 313, 362
Alw26I GTCTC 3 cut(s) 56, 64, 204
AoxI GGCC 3 cut(s) 166, 328, 508
ApeKI GCWGC 2 cut(s) 313, 362
ApoI RAATTY 1 cut(s) 404
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 328, 353
AsuHPI GGTGA 3 cut(s) 17, 76, 440
AvaII GGWCC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 300, 349
BccI CCATC 2 cut(s) 176, 200
BceAI ACGGC 2 cut(s) 350, 461
BciT130I CCWGG 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 56, 64, 204
BfoI RGCGCY 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 7 cut(s) 60, 104, 166, 314, 321, 363, 412
BlsI GCNGC 7 cut(s) 61, 105, 167, 315, 322, 364, 413
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 328, 353
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 1 cut(s) 247
BpmI CTGGAG 2 cut(s) 194, 353
BpuEI CTTGAG 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 201, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 517
Bse118I RCCGGY 1 cut(s) 260
Bse3DI GCAATG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 175, 517
BseMI GCAATG 1 cut(s) 511
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 2 cut(s) 300, 349
BseYI CCCAGC 3 cut(s) 295, 325, 516
BshFI GGCC 3 cut(s) 168, 330, 510
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 175, 517
BsmAI GTCTC 3 cut(s) 56, 64, 204
BsmBI CGTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 3 cut(s) 168, 330, 510
Bso31I GGTCTC 1 cut(s) 204
BspACI CCGC 7 cut(s) 60, 103, 165, 186, 245, 320, 411
BspANI GGCC 3 cut(s) 168, 330, 510
BspLI GGNNCC 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 170, 262, 318
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstMAI GTCTC 3 cut(s) 56, 64, 204
BstMWI GCNNNNNNNGC 4 cut(s) 65, 74, 192, 322
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 300, 349
BsuRI GGCC 3 cut(s) 168, 330, 510
BtgZI GCGATG 1 cut(s) 22
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 1 cut(s) 259
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 170, 262, 318
CfoI GCGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 2 cut(s) 328, 353
Csp6I GTAC 2 cut(s) 115, 305
CviAII CATG 1 cut(s) 32
CviJI RGCY 9 cut(s) 158, 168, 264, 295, 313, 330, 362, 448, 510
CviKI_1 RGCY 9 cut(s) 158, 168, 264, 295, 313, 330, 362, 448, 510
CviQI GTAC 2 cut(s) 115, 305
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eco130I CCWWGG 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 353
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 1 cut(s) 35
FaiI YATR 1 cut(s) 33
FaqI GGGAC 1 cut(s) 115
FatI CATG 1 cut(s) 31
FauI CCCGC 1 cut(s) 238
FblI GTMKAC 2 cut(s) 201, 219
Fnu4HI GCNGC 7 cut(s) 60, 104, 166, 314, 321, 363, 412
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 7 cut(s) 60, 104, 166, 314, 321, 363, 412
GlaI GCGC 1 cut(s) 324
GluI GCNGC 7 cut(s) 60, 104, 166, 314, 321, 363, 412
GsaI CCCAGC 3 cut(s) 299, 329, 520
GsuI CTGGAG 2 cut(s) 194, 353
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 3 cut(s) 168, 330, 510
HapII CCGG 1 cut(s) 261
HhaI GCGC 1 cut(s) 325
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 2 cut(s) 202, 220
HindII GTYRAC 2 cut(s) 202, 220
HinfI GANTC 4 cut(s) 72, 342, 374, 471
HpaII CCGG 1 cut(s) 261
HphI GGTGA 3 cut(s) 17, 76, 440
Hpy166II GTNNAC 2 cut(s) 202, 220
Hpy188I TCNGA 2 cut(s) 152, 422
Hpy8I GTNNAC 2 cut(s) 202, 220
Hpy99I CGWCG 4 cut(s) 206, 221, 224, 386
HpyAV CCTTC 2 cut(s) 8, 428
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 3 cut(s) 24, 128, 222
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 74, 192, 322
HpySE526I ACGT 3 cut(s) 24, 128, 222
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Lsp1109I GCAGC 2 cut(s) 300, 349
LweI GCATC 1 cut(s) 247
MaeII ACGT 3 cut(s) 24, 128, 222
MaeIII GTNAC 1 cut(s) 82
MboII GAAGA 1 cut(s) 538
MluCI AATT 1 cut(s) 404
MlyI GAGTC 2 cut(s) 66, 351
MmeI TCCRAC 1 cut(s) 448
MroNI GCCGGC 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 65, 74, 192, 322
NaeI GCCGGC 1 cut(s) 262
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 82
NspI RCATGY 1 cut(s) 35
PaqCI CACCTGC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 390
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 2 cut(s) 374, 471
PflFI GACNNNGTC 3 cut(s) 215, 224, 341
PkrI GCNGC 7 cut(s) 61, 105, 167, 315, 322, 364, 413
PleI GAGTC 2 cut(s) 66, 350
PpsI GAGTC 2 cut(s) 66, 350
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 3 cut(s) 295, 325, 516
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 328, 353
PspPPI RGGWCCY 1 cut(s) 353
PsyI GACNNNGTC 3 cut(s) 215, 224, 341
RsaI GTAC 2 cut(s) 116, 306
RsaNI GTAC 2 cut(s) 115, 305
SalI GTCGAC 2 cut(s) 200, 218
SatI GCNGC 7 cut(s) 60, 104, 166, 314, 321, 363, 412
Sau96I GGNCC 2 cut(s) 328, 353
SchI GAGTC 2 cut(s) 66, 351
ScrFI CCNGG 1 cut(s) 332
SetI ASST 9 cut(s) 27, 89, 131, 216, 225, 273, 306, 315, 364
SfaNI GCATC 1 cut(s) 247
SgrDI CGTCGACG 1 cut(s) 218
SinI GGWCC 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 404
SsiI CCGC 7 cut(s) 60, 103, 165, 186, 245, 320, 411
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 3 cut(s) 27, 131, 225
TaqI TCGA 3 cut(s) 138, 201, 219
TasI AATT 1 cut(s) 404
TauI GCSGC 5 cut(s) 62, 106, 168, 323, 414
TfiI GAWTC 2 cut(s) 374, 471
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 2 cut(s) 313, 362
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 2 cut(s) 38, 354
Tth111I GACNNNGTC 3 cut(s) 215, 224, 341
VpaK11BI GGWCC 1 cut(s) 353
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 201, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.