Rh7DG330400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0018248
Physical Location & Seq
Reverse (-)
40214480 .. 40214764
285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG330400.1

Sequence Viewer

Length: 285 bp
ATGAGCCTGCTGGAGGACGATGGCGGCGGCGGTGGTGGCGTTGTCGACGATGGAGACCTCGTCGACGTGGTCGGCGTTGATGAGGTCCTTGATGAGGGTGCGGGAGTGGATGATGCCGGCTTGGAGGTGGTTGAAGTAGAAGTGATCGGGCTGGGAGAGGTACTTGAGCTGCCAGCGGCGCTGGGCCTGGAGGACGGAGTCGGGGCAGGACCCGAAGCTGCCGTTGTTGATTCGGGCGACGGTGGGGTCGTGGTGGGAGAATTCGGCGGCGATTTCGGAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

8.9

Weight (kDa)

4.05

Isoelectric Point (pI)

22.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AccI GTMKAC 2 cut(s) 45, 63
AciI CCGC 6 cut(s) 24, 27, 30, 101, 176, 267
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 13, 94
AgsI TTSAA 1 cut(s) 134
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 2 cut(s) 169, 218
AluI AGCT 2 cut(s) 169, 218
Alw26I GTCTC 1 cut(s) 48
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 169, 218
ApoI RAATTY 1 cut(s) 260
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 85, 184, 209
AvaII GGWCC 2 cut(s) 85, 209
BbvI GCAGC 2 cut(s) 156, 205
BccI CCATC 2 cut(s) 14, 44
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 6 cut(s) 25, 28, 170, 177, 219, 268
BlsI GCNGC 6 cut(s) 26, 29, 171, 178, 220, 269
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 2 cut(s) 85, 209
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 3 cut(s) 85, 184, 209
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 1 cut(s) 103
BpmI CTGGAG 2 cut(s) 32, 209
BpuEI CTTGAG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 94
Bse118I RCCGGY 1 cut(s) 116
BseBI CCWGG 1 cut(s) 188
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 13, 94
BseXI GCAGC 2 cut(s) 156, 205
BseYI CCCAGC 2 cut(s) 151, 181
BshFI GGCC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 13, 94
BsmAI GTCTC 1 cut(s) 48
BsnI GGCC 1 cut(s) 186
Bso31I GGTCTC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 144
BspACI CCGC 6 cut(s) 24, 27, 30, 101, 176, 267
BspANI GGCC 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BssMI GATC 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 8, 118, 174
BstENI CCTNNNNNAGG 2 cut(s) 11, 92
BstF5I GGATG 1 cut(s) 115
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 36, 178
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 156, 205
BsuRI GGCC 1 cut(s) 186
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 3 cut(s) 8, 118, 174
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 85, 184, 209
Csp6I GTAC 1 cut(s) 161
CviJI RGCY 6 cut(s) 6, 120, 151, 169, 186, 218
CviKI_1 RGCY 6 cut(s) 6, 120, 151, 169, 186, 218
CviQI GTAC 1 cut(s) 161
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 85, 209
EcoNI CCTNNNNNAGG 2 cut(s) 11, 92
EcoO109I RGGNCCY 2 cut(s) 85, 209
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 186
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 2 cut(s) 45, 63
Fnu4HI GCNGC 6 cut(s) 25, 28, 170, 177, 219, 268
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 6 cut(s) 25, 28, 170, 177, 219, 268
GlaI GCGC 1 cut(s) 180
GluI GCNGC 6 cut(s) 25, 28, 170, 177, 219, 268
GsaI CCCAGC 2 cut(s) 155, 185
GsuI CTGGAG 2 cut(s) 32, 209
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 1 cut(s) 186
HapII CCGG 1 cut(s) 117
HhaI GCGC 1 cut(s) 181
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 2 cut(s) 46, 64
HindII GTYRAC 2 cut(s) 46, 64
HinfI GANTC 2 cut(s) 198, 230
HpaII CCGG 1 cut(s) 117
Hpy166II GTNNAC 2 cut(s) 46, 64
Hpy188I TCNGA 1 cut(s) 278
Hpy8I GTNNAC 2 cut(s) 46, 64
Hpy99I CGWCG 4 cut(s) 50, 65, 68, 242
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 178
HpySE526I ACGT 1 cut(s) 66
HspAI GCGC 1 cut(s) 179
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 144
LpnPI CCDG 8 cut(s) 20, 130, 137, 167, 173, 186, 192, 200
Lsp1109I GCAGC 2 cut(s) 156, 205
LweI GCATC 1 cut(s) 103
MaeII ACGT 1 cut(s) 66
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MluCI AATT 1 cut(s) 260
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 8 cut(s) 7, 68, 76, 88, 118, 151, 184, 272
MroNI GCCGGC 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 36, 178
NaeI GCCGGC 1 cut(s) 118
NdeII GATC 1 cut(s) 144
NgoMIV GCCGGC 1 cut(s) 116
NlaIV GGNNCC 1 cut(s) 211
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 246
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 3 cut(s) 59, 68, 197
PkrI GCNGC 6 cut(s) 26, 29, 171, 178, 220, 269
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PpuMI RGGWCCY 2 cut(s) 85, 209
Psp5II RGGWCCY 2 cut(s) 85, 209
Psp6I CCWGG 1 cut(s) 186
PspFI CCCAGC 2 cut(s) 151, 181
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 3 cut(s) 85, 184, 209
PspPPI RGGWCCY 2 cut(s) 85, 209
PsyI GACNNNGTC 3 cut(s) 59, 68, 197
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SalI GTCGAC 2 cut(s) 44, 62
SatI GCNGC 6 cut(s) 25, 28, 170, 177, 219, 268
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 3 cut(s) 85, 184, 209
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 188
SetI ASST 7 cut(s) 60, 69, 87, 129, 162, 171, 220
SfaNI GCATC 1 cut(s) 103
SgrDI CGTCGACG 1 cut(s) 62
SinI GGWCC 2 cut(s) 85, 209
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 1 cut(s) 260
SsiI CCGC 6 cut(s) 24, 27, 30, 101, 176, 267
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 69
TaqI TCGA 2 cut(s) 45, 63
TasI AATT 1 cut(s) 260
TauI GCSGC 4 cut(s) 27, 30, 179, 270
TfiI GAWTC 1 cut(s) 230
TseI GCWGC 2 cut(s) 169, 218
TspGWI ACGGA 1 cut(s) 210
Tth111I GACNNNGTC 3 cut(s) 59, 68, 197
VpaK11BI GGWCC 2 cut(s) 85, 209
XagI CCTNNNNNAGG 2 cut(s) 11, 92
XapI RAATTY 1 cut(s) 260
XmiI GTMKAC 2 cut(s) 45, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.