Rh6AG017500

OTU-like cysteine protease family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018587
Physical Location & Seq
Forward (+)
1774047 .. 1782734
8688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG017500.1

Sequence Viewer

Length: 885 bp
ATGCGACTGAATTGGGGTTTGGCTGATGGAGGCTTGCAACCATTTGAAGGAGGGGAAATGCACCATAAAACAGGGAGGGGTGAGATATTTGGAAAGGGAGACAACAGATTTTGGAAGAGTACTTGGCCGCATTTTCACACCCTCAGTGATATTATTGGAGATTTGGGTAAGCCTTTCACCCTCGTTTTGGTCCTCCAAGCCGCGGCGACATCAGCTCGGAGCAGCTCAATATGTCAAGATCAGATCCCAGTCATGAGTAGAGGACATTTCAGGATGAACAAAGAGGGGGATTCGGGGACCGACCAAGATAGTCAGCTCCAAAAAGAAGAAACGAAAAAACGAATGGAGCATATAAAAAAACAAAAGGAAACATTTCGTCAGATGACTCATATACCTCTAATTCTTCGAACGAATGCGGGAATAGAAATTCCCACAGAGAATGAAGTGAAATCAGGACATAAGCGTCTTTCAGACTATTTGCAATTGTATGGTTTGGCGGAGCGCATTGTCATGGGAGATGGCAACTGTCAGTATCGTGCCTTGTCAGATCAGATATATAGGACCGAAGACAATTATGATCATGTAAAAAAGAAAGTTATTGAACAGCTCAAGAAAATGCCACAGAACTACAGTGGGTTTATTCTTACTGATGAGTATCCTACTTTTGATGCTTATATAGAAAAAATAAGCAGTATTGGGGAATGGGGAGACAATTGCACATTGCAGGCGGCTGCTGATGCGTTTGGTGCTACGATATACGTATTCTCGATTGCTTACCCTGACGGTGTAATTGCTATTCCTCCCTCATCAAGGTCCACTAGAGTTTGTTACATAAGCCATTGGGCTGAGGTACATTACAATTCAGTATATCACAAGGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

294

Amino Acids

33.4

Weight (kDa)

6.64

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 172 - 261 5.7e-11 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018587)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0486081
rosa_laevigata RLG00000015407 RLG00000015416
rosa_multiflora Rmu_sc0002522.1_g000045
rosa_samantha Rh6AG017500 Rh6AG018300 Rh6AG018600 Rh6AG019200
rosa_wichuraiana Rw6G000100 Rw6G002130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 462
AccII CGCG 1 cut(s) 203
AciI CCGC 6 cut(s) 128, 201, 203, 416, 497, 728
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 426
AfaI GTAC 2 cut(s) 121, 852
AfiI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 810
AgsI TTSAA 2 cut(s) 47, 602
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 4 cut(s) 215, 225, 316, 607
AluI AGCT 4 cut(s) 215, 225, 316, 607
Alw26I GTCTC 2 cut(s) 93, 702
AlwI GGATC 1 cut(s) 238
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 222, 731
ApoI RAATTY 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 372
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 4 cut(s) 190, 297, 561, 813
AsuHPI GGTGA 2 cut(s) 92, 169
AsuII TTCGAA 1 cut(s) 406
AvaII GGWCC 4 cut(s) 190, 297, 561, 813
BbsI GAAGAC 1 cut(s) 573
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 2 cut(s) 234, 718
BccI CCATC 2 cut(s) 20, 512
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 577
BcoDI GTCTC 2 cut(s) 93, 702
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 628
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 6 cut(s) 128, 201, 204, 223, 729, 732
BlsI GCNGC 6 cut(s) 129, 202, 205, 224, 730, 733
BmcAI AGTACT 1 cut(s) 121
Bme18I GGWCC 4 cut(s) 190, 297, 561, 813
BmgT120I GGNCC 4 cut(s) 190, 297, 561, 813
BmiI GGNNCC 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 658, 727
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 593
BsaAI YACGTR 1 cut(s) 760
BsaBI GATNNNNATC 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 187, 202, 810
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 719
Bse8I GATNNNNATC 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 810
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 2 cut(s) 157, 837
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 234, 718
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 127
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 810
BsmAI GTCTC 2 cut(s) 93, 702
BsmFI GGGAC 1 cut(s) 310
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 406
Bsp143I GATC 4 cut(s) 238, 243, 547, 577
BspACI CCGC 6 cut(s) 128, 201, 203, 416, 497, 728
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 2 cut(s) 156, 838
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 1 cut(s) 238
BspT104I TTCGAA 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 719
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 4 cut(s) 238, 243, 547, 577
Bst4CI ACNGT 3 cut(s) 527, 632, 785
Bst6I CTCTTC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 760
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 2 cut(s) 35, 726
BstDEI CTNAG 2 cut(s) 143, 846
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 808
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 4 cut(s) 241, 246, 550, 580
BstMAI GTCTC 2 cut(s) 93, 702
BstMBI GATC 4 cut(s) 238, 243, 547, 577
BstMWI GCNNNNNNNGC 3 cut(s) 212, 737, 746
BstSFI CTRYAG 1 cut(s) 628
BstSNI TACGTA 1 cut(s) 760
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 234, 718
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 151, 637
Cac8I GCNNGC 2 cut(s) 35, 726
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 4 cut(s) 190, 297, 561, 813
Cfr42I CCGCGG 1 cut(s) 204
CseI GACGC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 120, 851
CviAII CATG 3 cut(s) 253, 511, 581
CviQI GTAC 2 cut(s) 120, 851
DdeI CTNAG 2 cut(s) 143, 846
DpnI GATC 4 cut(s) 240, 245, 549, 579
DpnII GATC 4 cut(s) 238, 243, 547, 577
DrdI GACNNNNNNGTC 1 cut(s) 462
DseDI GACNNNNNNGTC 1 cut(s) 462
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 512
Eco105I TACGTA 1 cut(s) 760
Eco47I GGWCC 4 cut(s) 190, 297, 561, 813
EcoNI CCTNNNNNAGG 1 cut(s) 808
FaeI CATG 3 cut(s) 256, 514, 584
FaqI GGGAC 1 cut(s) 310
FatI CATG 3 cut(s) 252, 510, 580
FauI CCCGC 1 cut(s) 409
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 6 cut(s) 128, 201, 204, 223, 729, 732
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 6 cut(s) 128, 201, 204, 223, 729, 732
FspBI CTAG 1 cut(s) 819
GlaI GCGC 1 cut(s) 503
GluI GCNGC 6 cut(s) 128, 201, 204, 223, 729, 732
HaeIII GGCC 1 cut(s) 127
HgaI GACGC 1 cut(s) 452
HhaI GCGC 1 cut(s) 504
Hin1II CATG 3 cut(s) 256, 514, 584
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HinfI GANTC 2 cut(s) 290, 385
HphI GGTGA 2 cut(s) 92, 169
Hpy166II GTNNAC 1 cut(s) 816
Hpy188I TCNGA 6 cut(s) 219, 243, 381, 472, 547, 552
Hpy188III TCNNGA 6 cut(s) 236, 253, 271, 453, 610, 766
Hpy8I GTNNAC 1 cut(s) 816
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 527, 632, 785
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 5 cut(s) 37, 61, 481, 717, 724
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 737, 746
HpyF3I CTNAG 2 cut(s) 143, 846
HpySE526I ACGT 1 cut(s) 759
Hsp92II CATG 3 cut(s) 256, 514, 584
HspAI GCGC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 577
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 4 cut(s) 238, 243, 547, 577
LmnI GCTCC 4 cut(s) 219, 321, 346, 499
LpnPI CCDG 6 cut(s) 57, 256, 261, 438, 710, 792
Lsp1109I GCAGC 2 cut(s) 234, 718
LweI GCATC 2 cut(s) 658, 727
MaeI CTAG 1 cut(s) 819
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 1 cut(s) 827
MalI GATC 4 cut(s) 240, 245, 549, 579
MboI GATC 4 cut(s) 238, 243, 547, 577
MboII GAAGA 4 cut(s) 127, 338, 395, 578
MfeI CAATTG 2 cut(s) 482, 712
MflI RGATCY 1 cut(s) 243
MluCI AATT 8 cut(s) 10, 399, 426, 482, 571, 712, 789, 859
MlyI GAGTC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 203
MunI CAATTG 2 cut(s) 482, 712
Mva1269I GAATGC 1 cut(s) 418
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 212, 737, 746
NdeII GATC 4 cut(s) 238, 243, 547, 577
NlaIII CATG 3 cut(s) 256, 514, 584
NlaIV GGNNCC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 406
PagI TCATGA 1 cut(s) 252
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 6 cut(s) 129, 202, 205, 224, 730, 733
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Ppu21I YACGTR 1 cut(s) 760
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 4 cut(s) 190, 297, 561, 813
PsuI RGATCY 1 cut(s) 243
RsaI GTAC 2 cut(s) 121, 852
RsaNI GTAC 2 cut(s) 120, 851
RseI CAYNNNNRTG 1 cut(s) 509
SacII CCGCGG 1 cut(s) 204
SatI GCNGC 6 cut(s) 128, 201, 204, 223, 729, 732
Sau3AI GATC 4 cut(s) 238, 243, 547, 577
Sau96I GGNCC 4 cut(s) 190, 297, 561, 813
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 1 cut(s) 379
SetI ASST 9 cut(s) 217, 227, 318, 397, 609, 762, 815, 852, 879
SfaNI GCATC 2 cut(s) 658, 727
SfcI CTRYAG 1 cut(s) 628
Sfr303I CCGCGG 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 406
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 4 cut(s) 190, 297, 561, 813
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SnaBI TACGTA 1 cut(s) 760
Sse9I AATT 8 cut(s) 10, 399, 426, 482, 571, 712, 789, 859
SsiI CCGC 6 cut(s) 128, 201, 203, 416, 497, 728
SspMI CTAG 1 cut(s) 819
TaaI ACNGT 3 cut(s) 527, 632, 785
TaiI ACGT 1 cut(s) 762
TaqI TCGA 2 cut(s) 406, 767
TaqII GACCGA 2 cut(s) 314, 578
TasI AATT 8 cut(s) 10, 399, 426, 482, 571, 712, 789, 859
TatI WGTACW 1 cut(s) 119
TauI GCSGC 4 cut(s) 130, 203, 206, 731
TfiI GAWTC 1 cut(s) 290
TscAI CASTG 2 cut(s) 151, 637
TseI GCWGC 2 cut(s) 222, 731
TspDTI ATGAA 2 cut(s) 290, 456
TspRI CASTG 2 cut(s) 151, 637
VpaK11BI GGWCC 4 cut(s) 190, 297, 561, 813
XagI CCTNNNNNAGG 1 cut(s) 808
XapI RAATTY 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 819
ZrmI AGTACT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.