Rh6AG018300

OTU-like cysteine protease family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018587
Physical Location & Seq
Forward (+)
1897915 .. 1905416
7502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG018300.1

Sequence Viewer

Length: 900 bp
ATGCGACTGAATTGGGGTTTGGCTGATGGAGGCTTGCAACCATTTGAAGGAGGGGAAATGCACCATAAAACAGGGAGGGGTGAGATATTTGGAAAGGGAGACAACAGATTTTGGAAGAGTACTTGGCCGCATTTTCACACCCTCAGTGATATTATTGGAGATTTGGGTAAGCCTTTCACCCTCGTTTTGGTCCTCCAAGCCGCGGCGACATCAGCTCGGAGCAGCTCAATATGTCAAGATCAGATCCCAGTCATGAACAAGCGAAAAAACTGTAGAGGACATTTCAGGATGAACAAAGAGGGGGATTCGGGGACCGACCAAGATAGTCAGCTCCAAAAAGAAGAAACGAAAAAACGAATGGAGCATATAAAAAAACAAAAGGAAACATTTCGTCAGATGACTCATATACCTCTAATTCTTCGAACGAATGCGGGAATAGAAATTCCCACAGAGAATGAAGTGAAATCAGGACATAAGCGTCTTTCAGACTATTTGCAATTGTATGGTTTGGCGGAGCGCATTGTCATGGGAGATGGCAACTGTCAGTATCGTGCCTTGTCAGATCAGATATATAGGACCGAAGACAATTATGATCATGTAAAAAAGAAAGTTATTGAACAGCTCAAGAAAATGCCACAGAACTACAGTGGGTTTATTCTTACTGATGAGTATCCTACTTTTGATGCTTATATAGAAAAAATAAGCAGTATTGGGGAATGGGGAGACAATTGCACATTGCAGGCGGCTGCTGATGCGTTTGGTGCTACGATATACGTATTCTCGATTGCTTACCCTGACGGTGTAATTGCTATTCCTCCCTCATCAAGGTCCACTAGAGTTTGTTACATAAGCCATTGGGCTGAGGTACATTACAATTCAGTATATCACAAGGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

299

Amino Acids

34.06

Weight (kDa)

7.7

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 177 - 266 5.9e-11 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018587)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0486081
rosa_laevigata RLG00000015407 RLG00000015416
rosa_multiflora Rmu_sc0002522.1_g000045
rosa_samantha Rh6AG017500 Rh6AG018300 Rh6AG018600 Rh6AG019200
rosa_wichuraiana Rw6G000100 Rw6G002130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 477
AccII CGCG 1 cut(s) 203
AciI CCGC 6 cut(s) 128, 201, 203, 431, 512, 743
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 441
AfaI GTAC 2 cut(s) 121, 867
AfiI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 825
AgsI TTSAA 2 cut(s) 47, 617
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 4 cut(s) 215, 225, 331, 622
AluI AGCT 4 cut(s) 215, 225, 331, 622
Alw26I GTCTC 2 cut(s) 93, 717
AlwI GGATC 1 cut(s) 238
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 222, 746
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 519
AspS9I GGNCC 4 cut(s) 190, 312, 576, 828
AsuHPI GGTGA 2 cut(s) 92, 169
AsuII TTCGAA 1 cut(s) 421
AvaII GGWCC 4 cut(s) 190, 312, 576, 828
BbsI GAAGAC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 2 cut(s) 234, 733
BccI CCATC 2 cut(s) 20, 527
BciVI GTATCC 1 cut(s) 681
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 93, 717
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 2 cut(s) 271, 643
BfuI GTATCC 1 cut(s) 681
BisI GCNGC 6 cut(s) 128, 201, 204, 223, 744, 747
BlsI GCNGC 6 cut(s) 129, 202, 205, 224, 745, 748
BmcAI AGTACT 1 cut(s) 121
Bme18I GGWCC 4 cut(s) 190, 312, 576, 828
BmgT120I GGNCC 4 cut(s) 190, 312, 576, 828
BmiI GGNNCC 1 cut(s) 313
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 673, 742
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 608
BsaAI YACGTR 1 cut(s) 775
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 187, 202, 825
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 734
Bse8I GATNNNNATC 1 cut(s) 669
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 294
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 825
BseMI GCAATG 1 cut(s) 734
BseMII CTCAG 2 cut(s) 157, 852
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 234, 733
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 127
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 4 cut(s) 47, 187, 202, 825
BsmAI GTCTC 2 cut(s) 93, 717
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 421
Bsp143I GATC 4 cut(s) 238, 243, 562, 592
BspACI CCGC 6 cut(s) 128, 201, 203, 431, 512, 743
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 2 cut(s) 156, 853
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 313
BspPI GGATC 1 cut(s) 238
BspT104I TTCGAA 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 734
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 4 cut(s) 238, 243, 562, 592
Bst4CI ACNGT 4 cut(s) 272, 542, 647, 800
Bst6I CTCTTC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 775
BstBI TTCGAA 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 35, 741
BstDEI CTNAG 2 cut(s) 143, 861
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 823
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 4 cut(s) 241, 246, 565, 595
BstMAI GTCTC 2 cut(s) 93, 717
BstMBI GATC 4 cut(s) 238, 243, 562, 592
BstMWI GCNNNNNNNGC 3 cut(s) 212, 752, 761
BstSFI CTRYAG 2 cut(s) 271, 643
BstSNI TACGTA 1 cut(s) 775
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 234, 733
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BsuI GTATCC 1 cut(s) 681
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 151, 652
Cac8I GCNNGC 2 cut(s) 35, 741
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 519
Cfr13I GGNCC 4 cut(s) 190, 312, 576, 828
Cfr42I CCGCGG 1 cut(s) 204
CseI GACGC 1 cut(s) 467
Csp6I GTAC 2 cut(s) 120, 866
CviAII CATG 3 cut(s) 253, 526, 596
CviQI GTAC 2 cut(s) 120, 866
DdeI CTNAG 2 cut(s) 143, 861
DpnI GATC 4 cut(s) 240, 245, 564, 594
DpnII GATC 4 cut(s) 238, 243, 562, 592
DrdI GACNNNNNNGTC 1 cut(s) 477
DseDI GACNNNNNNGTC 1 cut(s) 477
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 527
Eco105I TACGTA 1 cut(s) 775
Eco47I GGWCC 4 cut(s) 190, 312, 576, 828
EcoNI CCTNNNNNAGG 1 cut(s) 823
FaeI CATG 3 cut(s) 256, 529, 599
FaqI GGGAC 1 cut(s) 325
FatI CATG 3 cut(s) 252, 525, 595
FauI CCCGC 1 cut(s) 424
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 128, 201, 204, 223, 744, 747
FokI GGATG 1 cut(s) 301
Fsp4HI GCNGC 6 cut(s) 128, 201, 204, 223, 744, 747
FspBI CTAG 1 cut(s) 834
GlaI GCGC 1 cut(s) 518
GluI GCNGC 6 cut(s) 128, 201, 204, 223, 744, 747
HaeIII GGCC 1 cut(s) 127
HgaI GACGC 1 cut(s) 467
HhaI GCGC 1 cut(s) 519
Hin1II CATG 3 cut(s) 256, 529, 599
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HinfI GANTC 2 cut(s) 305, 400
HphI GGTGA 2 cut(s) 92, 169
Hpy166II GTNNAC 1 cut(s) 831
Hpy188I TCNGA 6 cut(s) 219, 243, 396, 487, 562, 567
Hpy188III TCNNGA 6 cut(s) 236, 253, 286, 468, 625, 781
Hpy8I GTNNAC 1 cut(s) 831
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 4 cut(s) 272, 542, 647, 800
HpyCH4IV ACGT 1 cut(s) 774
HpyCH4V TGCA 5 cut(s) 37, 61, 496, 732, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 752, 761
HpyF3I CTNAG 2 cut(s) 143, 861
HpySE526I ACGT 1 cut(s) 774
Hsp92II CATG 3 cut(s) 256, 529, 599
HspAI GCGC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 592
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 4 cut(s) 238, 243, 562, 592
LmnI GCTCC 4 cut(s) 219, 336, 361, 514
LpnPI CCDG 6 cut(s) 57, 261, 271, 453, 725, 807
Lsp1109I GCAGC 2 cut(s) 234, 733
LweI GCATC 2 cut(s) 673, 742
MaeI CTAG 1 cut(s) 834
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 1 cut(s) 842
MalI GATC 4 cut(s) 240, 245, 564, 594
MboI GATC 4 cut(s) 238, 243, 562, 592
MboII GAAGA 4 cut(s) 127, 353, 410, 593
MfeI CAATTG 2 cut(s) 497, 727
MflI RGATCY 1 cut(s) 243
MluCI AATT 8 cut(s) 10, 414, 441, 497, 586, 727, 804, 874
MlyI GAGTC 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 203
MunI CAATTG 2 cut(s) 497, 727
Mva1269I GAATGC 1 cut(s) 433
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 212, 752, 761
NdeII GATC 4 cut(s) 238, 243, 562, 592
NlaIII CATG 3 cut(s) 256, 529, 599
NlaIV GGNNCC 1 cut(s) 313
NspV TTCGAA 1 cut(s) 421
PagI TCATGA 1 cut(s) 252
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 6 cut(s) 129, 202, 205, 224, 745, 748
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 775
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 4 cut(s) 190, 312, 576, 828
PsuI RGATCY 1 cut(s) 243
RsaI GTAC 2 cut(s) 121, 867
RsaNI GTAC 2 cut(s) 120, 866
RseI CAYNNNNRTG 1 cut(s) 524
SacII CCGCGG 1 cut(s) 204
SatI GCNGC 6 cut(s) 128, 201, 204, 223, 744, 747
Sau3AI GATC 4 cut(s) 238, 243, 562, 592
Sau96I GGNCC 4 cut(s) 190, 312, 576, 828
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 1 cut(s) 394
SetI ASST 9 cut(s) 217, 227, 333, 412, 624, 777, 830, 867, 894
SfaNI GCATC 2 cut(s) 673, 742
SfcI CTRYAG 2 cut(s) 271, 643
Sfr303I CCGCGG 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 421
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 4 cut(s) 190, 312, 576, 828
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
SnaBI TACGTA 1 cut(s) 775
Sse9I AATT 8 cut(s) 10, 414, 441, 497, 586, 727, 804, 874
SsiI CCGC 6 cut(s) 128, 201, 203, 431, 512, 743
SspMI CTAG 1 cut(s) 834
TaaI ACNGT 4 cut(s) 272, 542, 647, 800
TaiI ACGT 1 cut(s) 777
TaqI TCGA 2 cut(s) 421, 782
TaqII GACCGA 2 cut(s) 329, 593
TasI AATT 8 cut(s) 10, 414, 441, 497, 586, 727, 804, 874
TatI WGTACW 1 cut(s) 119
TauI GCSGC 4 cut(s) 130, 203, 206, 746
TfiI GAWTC 1 cut(s) 305
TscAI CASTG 2 cut(s) 151, 652
TseI GCWGC 2 cut(s) 222, 746
TspDTI ATGAA 3 cut(s) 269, 305, 471
TspRI CASTG 2 cut(s) 151, 652
VpaK11BI GGWCC 4 cut(s) 190, 312, 576, 828
XagI CCTNNNNNAGG 1 cut(s) 823
XapI RAATTY 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 1 cut(s) 834
ZrmI AGTACT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.