Rw6G002130

OTU-like cysteine protease family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018587
Physical Location & Seq
Reverse (-)
3390289 .. 3395854
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002130.1

Sequence Viewer

Length: 1047 bp
ATGCTGTTTCAAGATATTAACCCTGTCATTTGCTTTCAGATTGTAAAAGTTTCTCTGGATCTACTCAAACTGTTCCTGAGATTTTGGAAGAGTACTTGGCCGCATTTTCAGACCCTCAGTGATATTATTGGAGATTTGGGTAAGCCTTTCACCCTCGTTTTGGTCCTCCAAGCCGCGGCGACATCAGCTCGTGTAGCATTAGTCATTCATGAAATTACGTTAGCCTTGGAAATCTATTGCTTCAGGAGAGAGCAGCTCAATATGTCAAGATCAGATCCCAGTCATGAGTATGCCCCCTTGGGTCTTCATCATCAATTGAATTTGTGCACTCTCACGTCCCATTGTCCTGATCGTCGCCCCAACCATTCAGACAAGCGAAAAAACTGTAGAGGACATTTCTGGATGAGCAAAGAGGGGGATTCGGGGACCGACCAAGATAGTCAGCTCCAAAAAGAAGAAACGAAAAAACAAATGGAGCATATAAAAAAACAAAAGGAAACATTTCGTCGGATGACTCATATACCTCTAATTCTTCGAACGAATGCGGGAATAGAAATTCCCACAGAGAATGAAGTGAAATCAGGACATAAGCGTCTTTCAGACTATTTGCAGTTGTATGGTTTGGCGGAGCGCATTGTCATGGGAGATGGCAACTGTCAGTATCGTGCCTTGTCAGATCAGATATATAGGACCGAAGACAATTATGATCATGTAAAAAAGAAAGTTATTGAACAGCTCAAGAAAATGCCTCAGAACTACAGTGGGTTTATTCTTACTGATGAGTATCCTACTTTTGATGCTTATATAGAAAAAATAAGCAGTATTGGGGAATGGGGAGACAATTGTACATTGCAGGCGGCTGCTGATGCGTTTGGTGCTACGATATACGTATTCTCAATTGCTTACCCTGATGGTGTAATTGCTATTCCTCCCTCATCAAGGTCCACTAGAGTGTGTTACATAAGCCATTGGGCTGAGGTACATTACAATTCAGTATATCACAAGAAAAAAAAAACAAAAGAAGAAAAAAGAAAACATTTGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

348

Amino Acids

40.19

Weight (kDa)

8.74

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 215 - 304 7.4e-11 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018587)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0486081
rosa_laevigata RLG00000015407 RLG00000015416
rosa_multiflora Rmu_sc0002522.1_g000045
rosa_samantha Rh6AG017500 Rh6AG018300 Rh6AG018600 Rh6AG019200
rosa_wichuraiana Rw6G000100 Rw6G002130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 591
AccII CGCG 1 cut(s) 176
AciI CCGC 6 cut(s) 101, 174, 176, 545, 626, 857
AclWI GGATC 2 cut(s) 66, 269
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 2 cut(s) 319, 555
AcuI CTGAAG 1 cut(s) 226
AfaI GTAC 3 cut(s) 94, 847, 981
AfiI CCNNNNNNNGG 3 cut(s) 160, 175, 939
AgsI TTSAA 3 cut(s) 11, 319, 731
AjiI CACGTC 1 cut(s) 336
AleI CACNNNNGTG 1 cut(s) 950
AluBI AGCT 4 cut(s) 188, 256, 445, 736
AluI AGCT 4 cut(s) 188, 256, 445, 736
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 831
Alw44I GTGCAC 1 cut(s) 325
AlwI GGATC 2 cut(s) 66, 269
AoxI GGCC 1 cut(s) 98
ApaLI GTGCAC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 253, 860
ApoI RAATTY 2 cut(s) 319, 555
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 633
AspS9I GGNCC 4 cut(s) 163, 426, 690, 942
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 535
AvaII GGWCC 4 cut(s) 163, 426, 690, 942
BaeGI GKGCMC 1 cut(s) 329
BauI CACGAG 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 296, 702
Bbv12I GWGCWC 1 cut(s) 329
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 2 cut(s) 265, 847
BccI CCATC 2 cut(s) 641, 905
BciVI GTATCC 1 cut(s) 795
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 831
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 2 cut(s) 385, 757
BfuI GTATCC 1 cut(s) 795
BisI GCNGC 6 cut(s) 101, 174, 177, 254, 858, 861
BlsI GCNGC 6 cut(s) 102, 175, 178, 255, 859, 862
BmcAI AGTACT 1 cut(s) 94
Bme18I GGWCC 4 cut(s) 163, 426, 690, 942
BmgBI CACGTC 1 cut(s) 336
BmgT120I GGNCC 4 cut(s) 163, 426, 690, 942
BmiI GGNNCC 1 cut(s) 427
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 2 cut(s) 787, 856
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 2 cut(s) 296, 702
BplI GAGNNNNNCTC 2 cut(s) 240, 272
Bpu10I CCTNAGC 1 cut(s) 975
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 722
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 3 cut(s) 174, 225, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 175, 939
Bse1I ACTGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 1 cut(s) 783
BseDI CCNNGG 3 cut(s) 174, 225, 297
BseGI GGATG 2 cut(s) 408, 516
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 160, 175, 939
BseMI GCAATG 1 cut(s) 848
BseMII CTCAG 4 cut(s) 68, 130, 764, 966
BseNI ACTGG 1 cut(s) 279
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 2 cut(s) 265, 847
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 329
BslFI GGGAC 2 cut(s) 322, 439
BslI CCNNNNNNNGG 3 cut(s) 160, 175, 939
BsmAI GTCTC 1 cut(s) 831
BsmFI GGGAC 2 cut(s) 322, 439
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 329
Bsp1407I TGTACA 1 cut(s) 845
Bsp143I GATC 6 cut(s) 58, 269, 274, 349, 676, 706
BspACI CCGC 6 cut(s) 101, 174, 176, 545, 626, 857
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 4 cut(s) 69, 129, 763, 967
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 2 cut(s) 208, 283
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 2 cut(s) 66, 269
BspT104I TTCGAA 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 848
BsrGI TGTACA 1 cut(s) 845
BsrI ACTGG 1 cut(s) 279
BssECI CCNNGG 3 cut(s) 174, 225, 297
BssMI GATC 6 cut(s) 58, 269, 274, 349, 676, 706
BssSI CACGAG 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 225, 297
Bst2BI CACGAG 1 cut(s) 189
Bst4CI ACNGT 4 cut(s) 72, 386, 656, 761
Bst6I CTCTTC 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 889
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 855
BstDEI CTNAG 4 cut(s) 77, 116, 750, 975
BstDSI CCRYGG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 937
BstF5I GGATG 2 cut(s) 408, 516
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 6 cut(s) 61, 272, 277, 352, 679, 709
BstMAI GTCTC 1 cut(s) 831
BstMBI GATC 6 cut(s) 58, 269, 274, 349, 676, 706
BstMWI GCNNNNNNNGC 4 cut(s) 185, 194, 866, 875
BstSFI CTRYAG 2 cut(s) 385, 757
BstSLI GKGCMC 1 cut(s) 329
BstSNI TACGTA 1 cut(s) 889
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 265, 847
BstV2I GAAGAC 2 cut(s) 296, 702
BstX2I RGATCY 2 cut(s) 58, 274
BstYI RGATCY 2 cut(s) 58, 274
BsuI GTATCC 1 cut(s) 795
BsuRI GGCC 1 cut(s) 100
BtgI CCRYGG 1 cut(s) 174
BtrI CACGTC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 408, 516
BtsIMutI CAGTG 2 cut(s) 124, 766
Cac8I GCNNGC 1 cut(s) 855
CciI TCATGA 2 cut(s) 208, 283
CfoI GCGC 1 cut(s) 633
Cfr13I GGNCC 4 cut(s) 163, 426, 690, 942
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 1 cut(s) 581
Csp6I GTAC 3 cut(s) 93, 846, 980
CviAII CATG 4 cut(s) 209, 284, 640, 710
CviQI GTAC 3 cut(s) 93, 846, 980
DdeI CTNAG 4 cut(s) 77, 116, 750, 975
DpnI GATC 6 cut(s) 60, 271, 276, 351, 678, 708
DpnII GATC 6 cut(s) 58, 269, 274, 349, 676, 706
DrdI GACNNNNNNGTC 1 cut(s) 591
DseDI GACNNNNNNGTC 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 641
Eco105I TACGTA 1 cut(s) 889
Eco130I CCWWGG 2 cut(s) 225, 297
Eco47I GGWCC 4 cut(s) 163, 426, 690, 942
Eco57I CTGAAG 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 937
EcoT14I CCWWGG 2 cut(s) 225, 297
ErhI CCWWGG 2 cut(s) 225, 297
FaeI CATG 4 cut(s) 212, 287, 643, 713
FaqI GGGAC 2 cut(s) 322, 439
FatI CATG 4 cut(s) 208, 283, 639, 709
FauI CCCGC 1 cut(s) 538
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 6 cut(s) 101, 174, 177, 254, 858, 861
FokI GGATG 2 cut(s) 415, 523
Fsp4HI GCNGC 6 cut(s) 101, 174, 177, 254, 858, 861
FspBI CTAG 1 cut(s) 948
GlaI GCGC 1 cut(s) 632
GluI GCNGC 6 cut(s) 101, 174, 177, 254, 858, 861
HaeIII GGCC 1 cut(s) 100
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 633
Hin1II CATG 4 cut(s) 212, 287, 643, 713
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HinfI GANTC 2 cut(s) 419, 514
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 327, 945
Hpy188I TCNGA 9 cut(s) 39, 111, 274, 370, 510, 601, 676, 681, 753
Hpy8I GTNNAC 2 cut(s) 327, 945
Hpy99I CGWCG 2 cut(s) 357, 510
HpyCH4III ACNGT 4 cut(s) 72, 386, 656, 761
HpyCH4IV ACGT 3 cut(s) 218, 335, 888
HpyCH4V TGCA 3 cut(s) 327, 610, 853
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 194, 866, 875
HpyF3I CTNAG 4 cut(s) 77, 116, 750, 975
HpySE526I ACGT 3 cut(s) 218, 335, 888
Hsp92II CATG 4 cut(s) 212, 287, 643, 713
HspAI GCGC 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 706
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 6 cut(s) 58, 269, 274, 349, 676, 706
LmnI GCTCC 3 cut(s) 450, 475, 628
Lsp1109I GCAGC 2 cut(s) 265, 847
LweI GCATC 2 cut(s) 787, 856
MaeI CTAG 1 cut(s) 948
MaeII ACGT 3 cut(s) 218, 335, 888
MaeIII GTNAC 1 cut(s) 956
MalI GATC 6 cut(s) 60, 271, 276, 351, 678, 708
MboI GATC 6 cut(s) 58, 269, 274, 349, 676, 706
MboII GAAGA 6 cut(s) 100, 296, 467, 524, 707, 1034
MfeI CAATTG 3 cut(s) 314, 841, 897
MflI RGATCY 2 cut(s) 58, 274
MhlI GDGCHC 1 cut(s) 329
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 2 cut(s) 18, 1045
MslI CAYNNNNRTG 3 cut(s) 288, 638, 950
MspA1I CMGCKG 1 cut(s) 176
MunI CAATTG 3 cut(s) 314, 841, 897
Mva1269I GAATGC 1 cut(s) 547
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 4 cut(s) 185, 194, 866, 875
NdeII GATC 6 cut(s) 58, 269, 274, 349, 676, 706
NlaIII CATG 4 cut(s) 212, 287, 643, 713
NlaIV GGNNCC 1 cut(s) 427
NspV TTCGAA 1 cut(s) 535
OliI CACNNNNGTG 1 cut(s) 950
PagI TCATGA 2 cut(s) 208, 283
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 6 cut(s) 102, 175, 178, 255, 859, 862
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 889
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 4 cut(s) 163, 426, 690, 942
PsuI RGATCY 2 cut(s) 58, 274
RsaI GTAC 3 cut(s) 94, 847, 981
RsaNI GTAC 3 cut(s) 93, 846, 980
RseI CAYNNNNRTG 3 cut(s) 288, 638, 950
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 2 cut(s) 18, 1045
SatI GCNGC 6 cut(s) 101, 174, 177, 254, 858, 861
Sau3AI GATC 6 cut(s) 58, 269, 274, 349, 676, 706
Sau96I GGNCC 4 cut(s) 163, 426, 690, 942
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 1 cut(s) 508
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 2 cut(s) 787, 856
SfcI CTRYAG 2 cut(s) 385, 757
Sfr303I CCGCGG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 535
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 4 cut(s) 163, 426, 690, 942
SmiMI CAYNNNNRTG 3 cut(s) 288, 638, 950
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
SnaBI TACGTA 1 cut(s) 889
SsiI CCGC 6 cut(s) 101, 174, 176, 545, 626, 857
SspMI CTAG 1 cut(s) 948
StyI CCWWGG 2 cut(s) 225, 297
TaaI ACNGT 4 cut(s) 72, 386, 656, 761
TaiI ACGT 3 cut(s) 221, 338, 891
TaqI TCGA 1 cut(s) 535
TaqII GACCGA 2 cut(s) 443, 707
TatI WGTACW 2 cut(s) 92, 845
TauI GCSGC 4 cut(s) 103, 176, 179, 860
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 2 cut(s) 18, 1045
Tru9I TTAA 2 cut(s) 18, 1045
TscAI CASTG 2 cut(s) 124, 766
TseI GCWGC 2 cut(s) 253, 860
TspDTI ATGAA 4 cut(s) 197, 225, 296, 585
TspRI CASTG 2 cut(s) 124, 766
VneI GTGCAC 1 cut(s) 325
VpaK11BI GGWCC 4 cut(s) 163, 426, 690, 942
XagI CCTNNNNNAGG 1 cut(s) 937
XapI RAATTY 2 cut(s) 319, 555
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 1 cut(s) 948
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.