Rh6AG366000

lysM domain-containing GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013375
Physical Location & Seq
Reverse (-)
56455611 .. 56458847
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG366000.1

Sequence Viewer

Length: 972 bp
ATGCAGCACCCAAAACCCTCTTACAAAACCCAGTCCTTCATTTTTTTCCTCTGGGTTTTAAGCAATGTACTTGTTCTCGCGACCTCAAAGTCCACAATCGAGCCCTGCTCGAACTCCGACTCCTGCAACGCTATGCTCGGCTACAAGGTCTACACCGAGCTCAAGGTCTCCGAAGTAGCTTCCCTCTTCCAGGTCGACCCCATCTCTATCCTCACCGCCAATGCCATCGACATTTCGTACCCCGACGTCGAAAACCACATTCTGCCCTCCGACCTCTTCCTCAAAATCCCAATCAACTGTTCATGCGTCGACGGAATCCGAAAATCGGTGTCGACCCACTACAAGACCCGGCCCTCCGACACCTTGGCCTCCATTGCCGACTCGGTCTACTCCGGTTTGGTGTCGGCGGACCAGATTCGGGAGGCTAATTCGATTTCGGACCCGTCGGTGCTGGATGTGGGACAGACGCTTGTGGTGCCTCTGCCGTGTACTTGCTTCAATGGGACGGATAATTCGTTGCCGGCGATTTACTTGTCGTATGTGGTGACCCCCGAGGACTCGCTGGCGGGGATTGCGGCCAGGTATTCGACTACGCTGACGGATTTGATGAATGTTAATGCCATGGGGAGCTCGGCTGTTAGGGCTTCTGATATTATTTCAATTCCATTGCCTGCCTGTGCATCAAACTTTCCTAGCTATGCTGCAGATCACGGCTTGATTGTGCCCAACGGGAGCTATGCCATTACAGCAAGTCACTGCCTTCAATGCAGTTGTGGTCCTGGGAGTCTAAATTTGTACTGTGAGCCTGCTTCATTAGCAGTTTCGTGTTCAAGCATGCAATGTAAAAACAGTAACCTCATGGTGGGCAACATTACAGCGCAGCAAACTAGTGCTGGTTGCAATGTTAGTTCTTGCAACTATGGTGGTTTCGTGAATGGCTCCATAATCACGACGTATGGTTTTCCTTTCTGA

Protein Analysis

323

Amino Acids

34.22

Weight (kDa)

4.84

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM2_CERK1_LYK3_4_5 PF23472 112 - 161 3.8e-08 LYK3/4/5 LysM2 domain
LysM PF01476 114 - 160 1.5e-09 LysM domain
LysM PF01476 180 - 222 2.1e-07 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21880 AT1G21880
fragaria_vesca FvH4_2g26750
malus_domestica MD08G1165500.v1.1 MD15G1351200.v1.1
prunus_persica Prupe.1G500000_v2.0.a1
pyrus_communis pycom12654g00100
rosa_chinensis RchiOBHm_Chr6g0295631
rosa_laevigata RLG00000011781
rosa_multiflora Rmu_sc0005000.1_g000002 Rmu_sc0006802.1_g000011
rosa_roxburghii Rroxscaffold_7G00172300
rosa_rugosa Rorug06G0253000
rosa_samantha Rh6AG366000 Rh6BG374100 Rh6CG380400 Rh6DG367200
rosa_wichuraiana Rw6G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AatII GACGTC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 475
AccI GTMKAC 5 cut(s) 150, 195, 309, 332, 387
AccII CGCG 1 cut(s) 80
AciI CCGC 4 cut(s) 216, 407, 566, 575
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 790
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 4 cut(s) 69, 239, 490, 797
AfiI CCNNNNNNNGG 4 cut(s) 190, 325, 418, 862
AgsI TTSAA 4 cut(s) 499, 660, 764, 831
AhlI ACTAGT 1 cut(s) 887
AjnI CCWGG 3 cut(s) 189, 578, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 5 cut(s) 160, 179, 630, 696, 735
AluI AGCT 5 cut(s) 160, 179, 630, 696, 735
Alw21I GWGCWC 2 cut(s) 162, 632
Alw26I GTCTC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 3 cut(s) 350, 366, 576
ApeKI GCWGC 3 cut(s) 4, 701, 880
ApoI RAATTY 1 cut(s) 790
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 4 cut(s) 351, 409, 439, 776
AsuC2I CCSGG 1 cut(s) 349
AsuHPI GGTGA 2 cut(s) 205, 556
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 3 cut(s) 409, 439, 776
BaeGI GKGCMC 1 cut(s) 726
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 3 cut(s) 105, 162, 632
Bbv12I GWGCWC 2 cut(s) 162, 632
BbvI GCAGC 3 cut(s) 16, 688, 892
BccI CCATC 2 cut(s) 209, 233
BceAI ACGGC 2 cut(s) 469, 727
BciT130I CCWGG 3 cut(s) 191, 580, 780
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 172
BcuI ACTAGT 1 cut(s) 887
BfaI CTAG 2 cut(s) 693, 888
BfmI CTRYAG 1 cut(s) 702
BisI GCNGC 4 cut(s) 5, 576, 702, 881
BlsI GCNGC 4 cut(s) 6, 577, 703, 882
Bme1390I CCNGG 4 cut(s) 191, 349, 580, 780
Bme18I GGWCC 3 cut(s) 409, 439, 776
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 351, 409, 439, 776
BmiI GGNNCC 3 cut(s) 441, 477, 940
BmrFI CCNGG 4 cut(s) 191, 349, 580, 780
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 92, 124
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 4 cut(s) 363, 552, 621, 779
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 325, 418, 862
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 31
Bse3DI GCAATG 5 cut(s) 70, 372, 665, 845, 907
BseBI CCWGG 3 cut(s) 191, 580, 780
BseDI CCNNGG 4 cut(s) 363, 552, 621, 779
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 4 cut(s) 190, 325, 418, 862
BseMI GCAATG 5 cut(s) 70, 372, 665, 845, 907
BseNI ACTGG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 726
BseXI GCAGC 3 cut(s) 16, 688, 892
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 3 cut(s) 352, 368, 578
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 2 cut(s) 162, 632
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 349, 393, 521
BslFI GGGAC 2 cut(s) 474, 517
BslI CCNNNNNNNGG 4 cut(s) 190, 325, 418, 862
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 474, 517
BsnI GGCC 3 cut(s) 352, 368, 578
Bso31I GGTCTC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 105, 162, 632, 726
Bsp143I GATC 1 cut(s) 706
Bsp19I CCATGG 1 cut(s) 621
Bsp68I TCGCGA 1 cut(s) 80
BspACI CCGC 4 cut(s) 216, 407, 566, 575
BspANI GGCC 3 cut(s) 352, 368, 578
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 3 cut(s) 441, 477, 940
BspMAI CTGCAG 1 cut(s) 706
BspT107I GGYRCC 1 cut(s) 475
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 5 cut(s) 70, 372, 665, 845, 907
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 4 cut(s) 363, 552, 621, 779
BssMI GATC 1 cut(s) 706
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 363, 621
Bst2UI CCWGG 3 cut(s) 191, 580, 780
Bst4CI ACNGT 3 cut(s) 299, 800, 851
Bst6I CTCTTC 2 cut(s) 191, 281
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 5 cut(s) 522, 564, 672, 807, 836
BstDSI CCRYGG 1 cut(s) 621
BstEII GGTNACC 1 cut(s) 544
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 1 cut(s) 709
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 1 cut(s) 706
BstMWI GCNNNNNNNGC 7 cut(s) 374, 475, 572, 641, 746, 765, 815
BstNI CCWGG 3 cut(s) 191, 580, 780
BstNSI RCATGY 1 cut(s) 838
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 4 cut(s) 189, 347, 578, 778
BstSFI CTRYAG 1 cut(s) 702
BstSLI GKGCMC 1 cut(s) 726
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 16, 688, 892
BsuRI GGCC 3 cut(s) 352, 368, 578
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 1 cut(s) 754
BtuMI TCGCGA 1 cut(s) 80
Cac8I GCNNGC 5 cut(s) 522, 564, 672, 807, 836
CfoI GCGC 1 cut(s) 880
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 4 cut(s) 351, 409, 439, 776
CseI GACGC 2 cut(s) 295, 475
Csp6I GTAC 4 cut(s) 68, 238, 489, 796
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 4 cut(s) 303, 622, 835, 859
CviQI GTAC 4 cut(s) 68, 238, 489, 796
DpnI GATC 1 cut(s) 708
DpnII GATC 1 cut(s) 706
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 191, 281
EarI CTCTTC 2 cut(s) 191, 281
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 2 cut(s) 160, 630
Eco130I CCWWGG 2 cut(s) 363, 621
Eco24I GRGCYC 3 cut(s) 105, 162, 632
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 3 cut(s) 409, 439, 776
Eco53kI GAGCTC 2 cut(s) 160, 630
Eco88I CYCGRG 1 cut(s) 551
Eco91I GGTNACC 1 cut(s) 544
EcoICRI GAGCTC 2 cut(s) 160, 630
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 544
EcoRII CCWGG 3 cut(s) 189, 578, 778
EcoT14I CCWWGG 2 cut(s) 363, 621
EcoT38I GRGCYC 3 cut(s) 105, 162, 632
ErhI CCWWGG 2 cut(s) 363, 621
FaeI CATG 4 cut(s) 306, 625, 838, 862
FaqI GGGAC 2 cut(s) 474, 517
FatI CATG 4 cut(s) 302, 621, 834, 858
FauI CCCGC 1 cut(s) 559
FblI GTMKAC 5 cut(s) 150, 195, 309, 332, 387
Fnu4HI GCNGC 4 cut(s) 5, 576, 702, 881
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 3 cut(s) 105, 162, 632
Fsp4HI GCNGC 4 cut(s) 5, 576, 702, 881
FspBI CTAG 2 cut(s) 693, 888
GlaI GCGC 1 cut(s) 879
GluI GCNGC 4 cut(s) 5, 576, 702, 881
HaeIII GGCC 3 cut(s) 352, 368, 578
HapII CCGG 3 cut(s) 349, 393, 521
HgaI GACGC 2 cut(s) 295, 475
HhaI GCGC 1 cut(s) 880
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 4 cut(s) 306, 625, 838, 862
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 3 cut(s) 196, 310, 333
HindII GTYRAC 3 cut(s) 196, 310, 333
HinfI GANTC 6 cut(s) 119, 315, 380, 415, 557, 784
HpaII CCGG 3 cut(s) 349, 393, 521
HphI GGTGA 2 cut(s) 205, 556
Hpy166II GTNNAC 7 cut(s) 93, 151, 196, 310, 333, 388, 489
Hpy188I TCNGA 8 cut(s) 118, 172, 271, 320, 358, 439, 649, 971
Hpy188III TCNNGA 4 cut(s) 79, 419, 931, 949
Hpy8I GTNNAC 7 cut(s) 93, 151, 196, 310, 333, 388, 489
Hpy99I CGWCG 6 cut(s) 248, 251, 311, 314, 448, 955
HpyAV CCTTC 2 cut(s) 46, 770
HpyCH4III ACNGT 3 cut(s) 299, 800, 851
HpyCH4IV ACGT 2 cut(s) 246, 953
HpyCH4V TGCA 8 cut(s) 4, 126, 680, 704, 768, 838, 900, 915
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 475, 572, 641, 746, 765, 815
HpySE526I ACGT 2 cut(s) 246, 953
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 4 cut(s) 306, 625, 838, 862
HspAI GCGC 1 cut(s) 878
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
Kzo9I GATC 1 cut(s) 706
LmnI GCTCC 3 cut(s) 627, 732, 944
Lsp1109I GCAGC 3 cut(s) 16, 688, 892
LweI GCATC 1 cut(s) 689
MaeI CTAG 2 cut(s) 693, 888
MaeII ACGT 2 cut(s) 246, 953
MaeIII GTNAC 3 cut(s) 544, 752, 851
MalI GATC 1 cut(s) 708
MboI GATC 1 cut(s) 706
MboII GAAGA 2 cut(s) 178, 268
MhlI GDGCHC 4 cut(s) 105, 162, 632, 726
MluCI AATT 4 cut(s) 427, 511, 660, 790
MlyI GAGTC 4 cut(s) 113, 374, 551, 793
MmeI TCCRAC 3 cut(s) 141, 294, 381
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 2 cut(s) 59, 615
MspI CCGG 3 cut(s) 349, 393, 521
MspR9I CCNGG 4 cut(s) 191, 349, 580, 780
MvaI CCWGG 3 cut(s) 191, 580, 780
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 7 cut(s) 374, 475, 572, 641, 746, 765, 815
NaeI GCCGGC 1 cut(s) 522
NciI CCSGG 1 cut(s) 349
NcoI CCATGG 1 cut(s) 621
NdeII GATC 1 cut(s) 706
NgoMIV GCCGGC 1 cut(s) 520
NlaIII CATG 4 cut(s) 306, 625, 838, 862
NlaIV GGNNCC 3 cut(s) 441, 477, 940
NmeAIII GCCGAG 2 cut(s) 117, 611
NmuCI GTSAC 2 cut(s) 544, 752
NruI TCGCGA 1 cut(s) 80
NspI RCATGY 1 cut(s) 838
PaeI GCATGC 1 cut(s) 838
PcsI WCGNNNNNNNCGW 2 cut(s) 512, 521
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 315, 415
PflFI GACNNNGTC 1 cut(s) 383
PkrI GCNGC 4 cut(s) 6, 577, 703, 882
PleI GAGTC 4 cut(s) 113, 374, 551, 792
PpsI GAGTC 4 cut(s) 113, 374, 551, 792
Psp124BI GAGCTC 2 cut(s) 162, 632
Psp6I CCWGG 3 cut(s) 189, 578, 778
PspEI GGTNACC 1 cut(s) 544
PspGI CCWGG 3 cut(s) 189, 578, 778
PspN4I GGNNCC 3 cut(s) 441, 477, 940
PspPI GGNCC 4 cut(s) 351, 409, 439, 776
PstI CTGCAG 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 383
RruI TCGCGA 1 cut(s) 80
RsaI GTAC 4 cut(s) 69, 239, 490, 797
RsaNI GTAC 4 cut(s) 68, 238, 489, 796
SacI GAGCTC 2 cut(s) 162, 632
SalI GTCGAC 3 cut(s) 194, 308, 331
SaqAI TTAA 2 cut(s) 59, 615
SatI GCNGC 4 cut(s) 5, 576, 702, 881
Sau3AI GATC 1 cut(s) 706
Sau96I GGNCC 4 cut(s) 351, 409, 439, 776
SchI GAGTC 4 cut(s) 113, 374, 551, 793
ScrFI CCNGG 4 cut(s) 191, 349, 580, 780
SduI GDGCHC 4 cut(s) 105, 162, 632, 726
SfaNI GCATC 1 cut(s) 689
SfcI CTRYAG 1 cut(s) 702
SgrDI CGTCGACG 1 cut(s) 308
SinI GGWCC 3 cut(s) 409, 439, 776
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SpeI ACTAGT 1 cut(s) 887
SphI GCATGC 1 cut(s) 838
Sse9I AATT 4 cut(s) 427, 511, 660, 790
SsiI CCGC 4 cut(s) 216, 407, 566, 575
SspMI CTAG 2 cut(s) 693, 888
SstI GAGCTC 2 cut(s) 162, 632
StyD4I CCNGG 4 cut(s) 189, 347, 578, 778
StyI CCWWGG 2 cut(s) 363, 621
TaaI ACNGT 3 cut(s) 299, 800, 851
TaiI ACGT 2 cut(s) 249, 956
TaqI TCGA 9 cut(s) 99, 110, 195, 228, 249, 309, 332, 431, 587
TaqII GACCGA 1 cut(s) 373
TasI AATT 4 cut(s) 427, 511, 660, 790
TatI WGTACW 3 cut(s) 67, 488, 795
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 2 cut(s) 315, 415
Tru1I TTAA 2 cut(s) 59, 615
Tru9I TTAA 2 cut(s) 59, 615
TscAI CASTG 1 cut(s) 761
TseFI GTSAC 2 cut(s) 544, 752
TseI GCWGC 3 cut(s) 4, 701, 880
Tsp45I GTSAC 2 cut(s) 544, 752
TspDTI ATGAA 4 cut(s) 28, 291, 623, 801
TspGWI ACGGA 3 cut(s) 327, 521, 614
TspRI CASTG 1 cut(s) 761
Tth111I GACNNNGTC 1 cut(s) 383
VpaK11BI GGWCC 3 cut(s) 409, 439, 776
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 1 cut(s) 790
XceI RCATGY 1 cut(s) 838
XmiI GTMKAC 5 cut(s) 150, 195, 309, 332, 387
XspI CTAG 2 cut(s) 693, 888
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.