Rh6BG236100

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018589
Physical Location & Seq
Reverse (-)
44260599 .. 44261948
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG236100.1

Sequence Viewer

Length: 879 bp
ATGCAAGAAGACGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCTGACAGCAAGAGAATCAGCAAAACTTCCTTGAAAAATTGGGTGGTCAATTGCTTTAGAGAACGGAGAGCACTTGCTTTGACTCTAACTGACACCAAAACAGCGGTGAACAGACTTCGACGCGTGGTGGACGTATTAGTTAGCATTATTATTATAATTATATGGCTGCTTGTGCTGGATATTGTCACCAGAGCCAATATTGTTTATGCAACTTCTCAGCTTGTGATTGTGGCCTTTGTTTTTGGTAACTCATGCAAGACAGTCTTTGAAGCAGTCATATTTCTATTCGTGATGCACCCCTTTGACGTGGGAGATCGATGTGAAATTGAAGGAGTTCAGATGGTTGTAGAGGAGATGAATATCTTAACTACAGTTTTTCTAAGGTATGACAACGTAAAAATTGTGTACCCAAACAGTACCCTTGCAACTAAGCCCATCAATAACTTCTATCGTAGTCCGGACTCAGGAGATGCTGTTGAGTTCTGTGTCCATATAGCCACTCCAGCAGATAAGATTGCTGTTATGAAGCAAAGAATGATAAGTTACATTGAAAATAAGAAGGAGCACTGGTATCCTGCACCAATGTTCATATTAAAGGAAGTGGAAGAATTAAATAGGATGAGGTTTGCCATATGGCTGTCACATAGAATGAACTTCCAAGACATGGGAGAAAAGTGGGCAAGGAGAGCCCTTTTACTTGAAGAGATAGTACGGATCCTCCAGGAGCTTGACATTGAGTATCGTTTGCGGCCCATTGACATAAATGTCCGTGGCATGCCTCCCACGGCCACCACTCTGCCTCCCTCTAATTTCACAGCAACCACAAGCTGA

Protein Analysis

292

Amino Acids

33.66

Weight (kDa)

7.66

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 109 - 165 2.2e-11 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018589)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0390441
rosa_multiflora Rmu_co8155526.1_g000001 Rmu_sc0003071.1_g000008 Rmu_sc0009053.1_g000002
rosa_samantha Rh4CG035500 Rh5AG363200 Rh6BG236100
rosa_wichuraiana Rw5G034190 Rw5G034250 Rw6G020250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AasI GACNNNNNNGTC 1 cut(s) 812
AccB7I CCANNNNNTGG 1 cut(s) 712
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 2 cut(s) 152, 796
AclWI GGATC 2 cut(s) 757, 770
AcoI YGGCCR 1 cut(s) 834
AfaI GTAC 3 cut(s) 455, 466, 759
AfiI CCNNNNNNNGG 2 cut(s) 512, 712
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 6 cut(s) 41, 82, 317, 377, 599, 749
AhdI GACNNNNNGTC 1 cut(s) 30
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 3 cut(s) 268, 775, 876
AluI AGCT 3 cut(s) 268, 775, 876
Alw21I GWGCWC 2 cut(s) 121, 615
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 2 cut(s) 757, 770
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 4 cut(s) 45, 279, 797, 834
ApeKI GCWGC 1 cut(s) 214
Asp700I GAANNNNTTC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 45, 798
AsuHPI GGTGA 2 cut(s) 166, 226
BamHI GGATCC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 739
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 121, 615
BbvI GCAGC 1 cut(s) 201
BccI CCATC 2 cut(s) 382, 491
BceAI ACGGC 1 cut(s) 849
BciT130I CCWGG 1 cut(s) 770
BciVI GTATCC 1 cut(s) 630
BcoDI GTCTC 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 417
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 2 cut(s) 215, 797
BlsI GCNGC 2 cut(s) 216, 798
Bme1390I CCNGG 1 cut(s) 770
BmeRI GACNNNNNGTC 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 45, 798
BmiI GGNNCC 2 cut(s) 46, 764
BmrFI CCNGG 1 cut(s) 770
BmsI GCATC 2 cut(s) 330, 508
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 2 cut(s) 534, 752
Bsa29I ATCGAT 1 cut(s) 364
BsaBI GATNNNNATC 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 817, 831
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 8 cut(s) 507, 537, 602, 632, 750, 780, 832, 862
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 712
Bse1I ACTGG 1 cut(s) 620
Bse8I GATNNNNATC 1 cut(s) 407
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 1 cut(s) 770
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 817, 831
BseGI GGATG 1 cut(s) 672
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 2 cut(s) 512, 712
BseMII CTCAG 2 cut(s) 278, 525
BseNI ACTGG 1 cut(s) 620
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 47, 281, 799, 836
BshVI ATCGAT 1 cut(s) 364
BsiHKAI GWGCWC 2 cut(s) 121, 615
BsiSI CCGG 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 512, 712
BsmAI GTCTC 1 cut(s) 38
BsnI GGCC 4 cut(s) 47, 281, 799, 836
Bsp1286I GDGCHC 3 cut(s) 121, 615, 739
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 2 cut(s) 361, 762
BspACI CCGC 2 cut(s) 152, 796
BspANI GGCC 4 cut(s) 47, 281, 799, 836
BspCNI CTCAG 2 cut(s) 277, 524
BspDI ATCGAT 1 cut(s) 364
BspEI TCCGGA 1 cut(s) 505
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 46, 764
BspPI GGATC 2 cut(s) 757, 770
BsrI ACTGG 1 cut(s) 620
BssECI CCNNGG 2 cut(s) 817, 831
BssMI GATC 2 cut(s) 361, 762
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 3 cut(s) 310, 421, 464
Bst6I CTCTTC 1 cut(s) 744
BstC8I GCNNGC 1 cut(s) 824
BstDEI CTNAG 4 cut(s) 264, 428, 477, 511
BstDSI CCRYGG 2 cut(s) 817, 831
BstF5I GGATG 1 cut(s) 672
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 2 cut(s) 364, 765
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 2 cut(s) 361, 762
BstMWI GCNNNNNNNGC 3 cut(s) 220, 551, 734
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 762
BstYI RGATCY 1 cut(s) 762
Bsu15I ATCGAT 1 cut(s) 364
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 4 cut(s) 47, 281, 799, 836
BsuTUI ATCGAT 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 817, 831
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 613
Cac8I GCNNGC 1 cut(s) 824
Cfr13I GGNCC 2 cut(s) 45, 798
ClaI ATCGAT 1 cut(s) 364
CseI GACGC 1 cut(s) 177
Csp6I GTAC 3 cut(s) 454, 465, 758
CviAII CATG 4 cut(s) 28, 300, 712, 823
CviQI GTAC 3 cut(s) 454, 465, 758
DdeI CTNAG 4 cut(s) 264, 428, 477, 511
DpnI GATC 2 cut(s) 363, 764
DpnII GATC 2 cut(s) 361, 762
DraI TTTAAA 1 cut(s) 21
DrdI GACNNNNNNGTC 1 cut(s) 812
DriI GACNNNNNGTC 1 cut(s) 30
DseDI GACNNNNNNGTC 1 cut(s) 812
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 744
Eam1105I GACNNNNNGTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 744
Eco24I GRGCYC 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 768
EcoT38I GRGCYC 1 cut(s) 739
FaeI CATG 4 cut(s) 31, 303, 715, 826
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 4 cut(s) 27, 299, 711, 822
FauNDI CATATG 1 cut(s) 680
Fnu4HI GCNGC 2 cut(s) 215, 797
FokI GGATG 1 cut(s) 679
FriOI GRGCYC 1 cut(s) 739
Fsp4HI GCNGC 2 cut(s) 215, 797
GluI GCNGC 2 cut(s) 215, 797
GsuI CTGGAG 2 cut(s) 534, 752
HaeIII GGCC 4 cut(s) 47, 281, 799, 836
HapII CCGG 1 cut(s) 506
HgaI GACGC 1 cut(s) 177
Hin1II CATG 4 cut(s) 31, 303, 715, 826
HinfI GANTC 4 cut(s) 31, 63, 130, 509
HpaII CCGG 1 cut(s) 506
HphI GGTGA 2 cut(s) 166, 226
Hpy166II GTNNAC 3 cut(s) 157, 178, 454
Hpy188I TCNGA 2 cut(s) 52, 387
Hpy188III TCNNGA 3 cut(s) 337, 506, 513
Hpy8I GTNNAC 3 cut(s) 157, 178, 454
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 3 cut(s) 35, 371, 601
HpyCH4III ACNGT 3 cut(s) 310, 421, 464
HpyCH4IV ACGT 3 cut(s) 180, 354, 441
HpyCH4V TGCA 6 cut(s) 4, 257, 303, 343, 473, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 551, 734
HpyF3I CTNAG 4 cut(s) 264, 428, 477, 511
HpySE526I ACGT 3 cut(s) 180, 354, 441
Hsp92II CATG 4 cut(s) 31, 303, 715, 826
Kpn2I TCCGGA 1 cut(s) 505
Kzo9I GATC 2 cut(s) 361, 762
LmnI GCTCC 2 cut(s) 610, 772
LpnPI CCDG 9 cut(s) 209, 250, 498, 519, 564, 601, 636, 755, 782
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 2 cut(s) 330, 508
MaeII ACGT 3 cut(s) 180, 354, 441
MaeIII GTNAC 4 cut(s) 232, 293, 590, 687
MalI GATC 2 cut(s) 363, 764
MboI GATC 2 cut(s) 361, 762
MboII GAAGA 3 cut(s) 20, 665, 761
MfeI CAATTG 1 cut(s) 97
MflI RGATCY 1 cut(s) 762
MhlI GDGCHC 3 cut(s) 121, 615, 739
MluCI AATT 7 cut(s) 85, 97, 204, 372, 447, 656, 856
MluI ACGCGT 1 cut(s) 169
MlyI GAGTC 3 cut(s) 40, 124, 503
MnlI CCTC 8 cut(s) 7, 58, 391, 663, 776, 837, 858, 862
MroI TCCGGA 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 4 cut(s) 20, 413, 641, 659
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 1 cut(s) 770
MunI CAATTG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 770
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 220, 551, 734
NdeI CATATG 1 cut(s) 680
NdeII GATC 2 cut(s) 361, 762
NlaIII CATG 4 cut(s) 31, 303, 715, 826
NlaIV GGNNCC 2 cut(s) 46, 764
NmuCI GTSAC 2 cut(s) 232, 687
NspI RCATGY 1 cut(s) 826
PaeI GCATGC 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 712
PfoI TCCNGGA 1 cut(s) 768
PkrI GCNGC 2 cut(s) 216, 798
PleI GAGTC 3 cut(s) 39, 124, 503
PpsI GAGTC 3 cut(s) 39, 124, 503
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 768
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 2 cut(s) 46, 764
PspPI GGNCC 2 cut(s) 45, 798
PsuI RGATCY 1 cut(s) 762
RsaI GTAC 3 cut(s) 455, 466, 759
RsaNI GTAC 3 cut(s) 454, 465, 758
SaqAI TTAA 4 cut(s) 20, 413, 641, 659
SatI GCNGC 2 cut(s) 215, 797
Sau3AI GATC 2 cut(s) 361, 762
Sau96I GGNCC 2 cut(s) 45, 798
SchI GAGTC 3 cut(s) 40, 124, 503
ScrFI CCNGG 1 cut(s) 770
SduI GDGCHC 3 cut(s) 121, 615, 739
SetI ASST 8 cut(s) 183, 270, 357, 434, 444, 674, 777, 878
SfaNI GCATC 2 cut(s) 330, 508
SfcI CTRYAG 1 cut(s) 417
SphI GCATGC 1 cut(s) 826
Sse9I AATT 7 cut(s) 85, 97, 204, 372, 447, 656, 856
SsiI CCGC 2 cut(s) 152, 796
SspI AATATT 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 768
TaaI ACNGT 3 cut(s) 310, 421, 464
TaiI ACGT 3 cut(s) 183, 357, 444
TaqI TCGA 2 cut(s) 166, 364
TasI AATT 7 cut(s) 85, 97, 204, 372, 447, 656, 856
TauI GCSGC 1 cut(s) 799
TfiI GAWTC 1 cut(s) 63
Tru1I TTAA 4 cut(s) 20, 413, 641, 659
Tru9I TTAA 4 cut(s) 20, 413, 641, 659
TscAI CASTG 1 cut(s) 620
TseFI GTSAC 2 cut(s) 232, 687
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 2 cut(s) 232, 687
TspDTI ATGAA 4 cut(s) 419, 587, 625, 713
TspGWI ACGGA 3 cut(s) 127, 775, 806
TspRI CASTG 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 712
XceI RCATGY 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.