Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0016680

Physical Location & Seq

Genomic Coordinates Forward (+)

67495137 .. 67497395

Gene Structure 2259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG466000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGACCCGGCAAGTTCTCAATCTCTCTGACCATTTGCTTCCCAAACTCCTCCACTTGTTAAACCCCTCATCTTCGTTATCACAGGCCCAACAAGCCCATGCCCAGATTCTGAAAACAGGTCTCTCCAATTACACCACCAAACTCCTTTCTCTCTACGCCAACACCCTCTGCTTTGCAGAAGCCAAACTCGTCCTCGATTCGATCCCACACCCCAACTTGTTCTCTTTCTCCACCCTCATTCATGCCTTCTCAAAGCTCAATAGCTTCGGCAATGCACTTGCCTTGTTCTCCCAAATGTTATCTCGGGGCCTCGTCCCGGATAGCTTCCTCTTCCCCAGTGTCGTCAAGGCCTGTGCCGGATTGCAATCTTCGCACTCCGCCAAACAGGTCCATGCGATTTCCTTTGCTTCCGGGTTTGGTTTCGATTCGTTTGTGCAATCCTCTTTGGTGCACATGTATATCAAATGTGACCGAATGAGGGATGCACAGAAGGTGTTTGATAGAGTGCCTGAGAGAGATGTGATTATTTACAGTGCTTTGATTTCTGGGTATTCTCGCCGCGGGTGTGTTGATGAGGCCCTGAAGCTTTTAGATGAGATGAGGGGTTTGGGGTTTGAGCCAAATGTGGTGTTGTGGAATGGGATGATTGCCGGGTTTAGTCGGAGCAAGTTGTATGCTAGTACAGTCGCCGTGCTTCAGAAAATGCATTCGGAAGGATTTGAACCCGATGGCTCTAGTATTTCTAGTGTTCTTCCTGCTGTAGGGGACTTGGGGGGTTTAGACGTTGGGATTCAGATTCATGGCTATGTCATCAAGCGGGGACTAAAGTCGGACAAGTGTGTTGTTAGTGCACTTGTCGATATGTATGGGAAGTGTGCCTGCACGTCGGAGATGTCTCGAGTTGTTGGTGAAATGGAGGAAATAGATGTTGGTGCTTGCAATGCTTTAGTTACAGGGCTTGCCCGAAATGGTCTTGTTGACAATGCACTGGAGGTGTTCAGGCAATTTAAGGGCAAAGGAGTGGAACTCAATATTGTGTCTTGGACCTCCATAATTGCTAGTTGTTCCCAAAATGGGAAGGATATGGAGGCTTTGGAGCTTTTTAGAGAGATGCAGATCGAGGGTGTGGAGCCAAACTCTATGACTATTTCTTGCTTGCTTCCGGCTTGTGGCAATATTGCAGCCTTGATGTATGGGAAGGCAGCCCATTGTTTCGCTCTCAGGAGGGGGATGCTTAGTGATGTGTATGTGGGCAGTGCATTGATTGATATGTATGCAAACTGCGGCAAAATCCTATTATCTCGACTTTGTTTTGATAAAATGCCCACTAGGAATTTGGTTTGCTGGAATGCAGTTATGAGCGGATATGCAATGCATGGAAAAGCTAAGGAAACTATGGAGATCTTTCGTATGATGCAGAGGAGTGGACAGAAGCCCGATATCATCAGCTTCACTTGTTTATTGTCTGCATGCAGCCAGAACGGTCTAACAGAAGATGGATGGTATTATTTTAACAGCATGTCAAAAGAACACAGTATAGAAGCCCGGATAGAACATTACGCTTGCATGGTCACACTACTCGGTCGTGCTGGAAAACTTGATGAGGCTTATTCCATGATCAAGAAAATGCCATTTGAACCTGATGCTTGTGTTTGGGGAGCTTTGCTAAGTTCTTGTAGAGTTCACAACAATGTGACTTTAGGGGAGTCTACTGCAAAGAAGCTCTTCAATTTAGAACCGGAAAATCCTGGGAACTACATTCTGTTGTCCAATATTTATGCTTCAAAGGGCATGTGGACTGAAGTAGATAGAGTGAGAGATACGATGAAGAGTCTGGGATTGAGGAAGAACCCTGGCTGCAGTTGGATCGAATTCAAGAACAAAGTTCACATGCTTCTTGTAGGAGACAAAGCACATCCTCAAATGAACCAAATTACGGAAAAGTTGAACAGATTGAGCTCAGAGATGAAGAGATCAGGCTACCTCCCCAATACCCACTTTGTATTGCAAGATGTGGAAGAACAAGAGAAGGAGCAGATTTTGTGTGGACACAGTGAGAAATTGGCAGTAGTGTTGGGGCTTCTGAACACTCCTCCAGGGTCTTCCCTTAGGGTAATTAAGAACCTCAGGATCTGTGGTGATTGTCATTCTGTTATCAAATTCATATCCAGCTTGGAAGGGAGAGAGATTTCAGTCAGAGACACCAATCGCTTTCATCATTTTAAAGATGGAGTTTGTTCTTGTGGGGATTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

752

Amino Acids

83.15

Weight (kDa)

8.16

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	71 - 119	1.9e-06	PPR repeat family
PPR_2	PF13041	172 - 210	5.8e-09	PPR repeat family
PPR_1	PF12854	172 - 201	4.2e-08	PPR repeat
PPR	PF01535	176 - 205	1.6e-08	PPR repeat
PPR_3	PF13812	332 - 392	7.1e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	344 - 391	2.4e-10	PPR repeat family
PPR	PF01535	346 - 376	1.1e-06	PPR repeat
PPR_2	PF13041	445 - 492	4.5e-11	PPR repeat family
PPR	PF01535	447 - 476	4e-06	PPR repeat
E_motif	PF20431	562 - 624	4.2e-25	E motif
Eplus_motif	PF20430	629 - 656	2.3e-06	E+ motif
DYW_deaminase	PF14432	660 - 752	2e-44	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20230
fragaria_vesca	FvH4_2g38000
malus_domestica	MD15G1007100.v1.1
prunus_persica	Prupe.1G360000_v2.0.a1
pyrus_communis	pycom15g00570
rosa_chinensis	RchiOBHm_Chr6g0303611
rosa_laevigata	RLG00000011068
rosa_multiflora	Rmu_ssc0000114.1_g000022
rosa_roxburghii	Rroxscaffold_7G00164580
rosa_rugosa	Rorug06G0322800
rosa_samantha	Rh6AG437300 Rh6BG466000 Rh6DG436000
rosa_wichuraiana	Rw6G037850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1362
AccI	GTMKAC	1 cut(s)	1709
AccII	CGCG	1 cut(s)	561
AciI	CCGC	6 cut(s)	378, 559, 561, 817, 1284, 1362
AclWI	GGATC	3 cut(s)	196, 1874, 2138
AcsI	RAATTY	3 cut(s)	1333, 1871, 2159
AcuI	CTGAAG	3 cut(s)	602, 680, 1821
AfaI	GTAC	1 cut(s)	682
AfiI	CCNNNNNNNGG	6 cut(s)	316, 478, 761, 1074, 1169, 1936
AflIII	ACRYGT	1 cut(s)	453
AgsI	TTSAA	6 cut(s)	722, 1637, 1729, 1785, 1876, 1948
AjiI	CACGTC	1 cut(s)	885
AjnI	CCWGG	3 cut(s)	1747, 1852, 2095
AjuI	GAANNNNNNNTTGG	2 cut(s)	912, 944
Alw21I	GWGCWC	3 cut(s)	453, 853, 1961
Alw26I	GTCTC	4 cut(s)	125, 900, 1899, 2193
Alw44I	GTGCAC	2 cut(s)	449, 849
AlwI	GGATC	3 cut(s)	196, 1874, 2138
AlwNI	CAGNNNCTG	2 cut(s)	109, 2133
Ama87I	CYCGRG	2 cut(s)	303, 897
AoxI	GGCC	4 cut(s)	84, 307, 348, 576
ApaLI	GTGCAC	2 cut(s)	449, 849
ApeKI	GCWGC	4 cut(s)	1181, 1202, 1473, 1857
ApoI	RAATTY	3 cut(s)	1333, 1871, 2159
Asp700I	GAANNNNTTC	1 cut(s)	1724
AspS9I	GGNCC	5 cut(s)	85, 307, 388, 577, 1044
AsuC2I	CCSGG	5 cut(s)	7, 317, 412, 652, 1546
AsuHPI	GGTGA	2 cut(s)	920, 2150
AvaI	CYCGRG	2 cut(s)	303, 897
AvaII	GGWCC	2 cut(s)	388, 1044
AxyI	CCTNAGG	2 cut(s)	2108, 2126
BaeGI	GKGCMC	2 cut(s)	453, 853
BanII	GRGCYC	1 cut(s)	1961
BbsI	GAAGAC	1 cut(s)	2094
Bbv12I	GWGCWC	3 cut(s)	453, 853, 1961
BbvI	GCAGC	4 cut(s)	1193, 1214, 1485, 1844
BccI	CCATC	4 cut(s)	722, 1490, 1494, 2222
BceAI	ACGGC	1 cut(s)	674
BcgI	CGANNNNNNTGC	2 cut(s)	1849, 1883
BciT130I	CCWGG	3 cut(s)	1749, 1854, 2097
BclI	TGATCA	1 cut(s)	1617
BcnI	CCSGG	5 cut(s)	7, 317, 412, 652, 1546
BcoDI	GTCTC	4 cut(s)	125, 900, 1899, 2193
BfaI	CTAG	5 cut(s)	678, 735, 744, 1059, 1329
BfmI	CTRYAG	2 cut(s)	759, 1858
BglII	AGATCT	1 cut(s)	1401
BisI	GCNGC	6 cut(s)	559, 1182, 1203, 1285, 1474, 1858
BlsI	GCNGC	6 cut(s)	560, 1183, 1204, 1286, 1475, 1859
Bme1390I	CCNGG	8 cut(s)	7, 317, 412, 652, 1546, 1749, 1854, 2097
Bme18I	GGWCC	2 cut(s)	388, 1044
BmeT110I	CYCGRG	2 cut(s)	303, 897
BmgBI	CACGTC	1 cut(s)	885
BmgT120I	GGNCC	5 cut(s)	85, 307, 388, 577, 1044
BmiI	GGNNCC	2 cut(s)	308, 1131
BmrFI	CCNGG	8 cut(s)	7, 317, 412, 652, 1546, 1749, 1854, 2097
BmrI	ACTGGG	1 cut(s)	330
BmsI	GCATC	5 cut(s)	472, 1101, 1221, 1404, 1633
BmuI	ACTGGG	1 cut(s)	330
BoxI	GACNNNNGTC	1 cut(s)	826
BpiI	GAAGAC	1 cut(s)	2094
BplI	GAGNNNNNCTC	4 cut(s)	1011, 1043, 1121, 1153
BpmI	CTGGAG	2 cut(s)	1010, 2079
Bpu10I	CCTNAGC	1 cut(s)	1386
BpuMI	CCSGG	5 cut(s)	7, 317, 412, 652, 1546
BsaBI	GATNNNNATC	2 cut(s)	364, 1115
BsaI	GGTCTC	1 cut(s)	125
BsaJI	CCNNGG	4 cut(s)	559, 1748, 1852, 2096
BsaWI	WCCGGW	1 cut(s)	1738
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 478, 761, 1074, 1169, 1936
Bse1I	ACTGG	3 cut(s)	336, 993, 2258
Bse21I	CCTNAGG	2 cut(s)	2108, 2126
Bse3DI	GCAATG	3 cut(s)	277, 946, 1377
Bse8I	GATNNNNATC	2 cut(s)	364, 1115
BseBI	CCWGG	3 cut(s)	1749, 1854, 2097
BseDI	CCNNGG	4 cut(s)	559, 1748, 1852, 2096
BseGI	GGATG	5 cut(s)	487, 648, 1236, 1505, 1915
BseJI	GATNNNNATC	2 cut(s)	364, 1115
BseLI	CCNNNNNNNGG	6 cut(s)	316, 478, 761, 1074, 1169, 1936
BseMI	GCAATG	3 cut(s)	277, 946, 1377
BseMII	CTCAG	4 cut(s)	501, 1234, 1974, 2140
BseNI	ACTGG	3 cut(s)	336, 993, 2258
BseRI	GAGGAG	3 cut(s)	38, 1435, 2082
BseSI	GKGCMC	2 cut(s)	453, 853
BseXI	GCAGC	4 cut(s)	1193, 1214, 1485, 1844
BsgI	GTGCAG	1 cut(s)	865
Bsh1236I	CGCG	1 cut(s)	561
Bsh1285I	CGRYCG	1 cut(s)	1585
BshFI	GGCC	4 cut(s)	86, 309, 350, 578
BsiEI	CGRYCG	1 cut(s)	1585
BsiHKAI	GWGCWC	3 cut(s)	453, 853, 1961
BsiHKCI	CYCGRG	2 cut(s)	303, 897
BsiSI	CCGG	8 cut(s)	7, 317, 357, 411, 651, 1163, 1546, 1739
BslFI	GGGAC	3 cut(s)	299, 779, 834
BslI	CCNNNNNNNGG	6 cut(s)	316, 478, 761, 1074, 1169, 1936
BsmAI	GTCTC	4 cut(s)	125, 900, 1899, 2193
BsmFI	GGGAC	3 cut(s)	299, 779, 834
BsmI	GAATGC	2 cut(s)	706, 1354
BsnI	GGCC	4 cut(s)	86, 309, 350, 578
Bso31I	GGTCTC	1 cut(s)	125
BsoBI	CYCGRG	2 cut(s)	303, 897
Bsp1286I	GDGCHC	3 cut(s)	453, 853, 1961
Bsp143I	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
BspACI	CCGC	6 cut(s)	378, 559, 561, 817, 1284, 1362
BspANI	GGCC	4 cut(s)	86, 309, 350, 578
BspCNI	CTCAG	4 cut(s)	502, 1233, 1973, 2139
BspFNI	CGCG	1 cut(s)	561
BspLI	GGNNCC	2 cut(s)	308, 1131
BspMAI	CTGCAG	1 cut(s)	1862
BspPI	GGATC	3 cut(s)	196, 1874, 2138
BspQI	GCTCTTC	1 cut(s)	1730
BspTNI	GGTCTC	1 cut(s)	125
BsrBI	CCGCTC	1 cut(s)	1362
BsrDI	GCAATG	3 cut(s)	277, 946, 1377
BsrI	ACTGG	3 cut(s)	336, 993, 2258
BssECI	CCNNGG	4 cut(s)	559, 1748, 1852, 2096
BssMI	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
Bst2UI	CCWGG	3 cut(s)	1749, 1854, 2097
Bst4CI	ACNGT	5 cut(s)	533, 685, 1484, 1535, 2054
Bst6I	CTCTTC	4 cut(s)	335, 1730, 1823, 1964
BstC8I	GCNNGC	6 cut(s)	880, 937, 960, 1157, 1471, 1564
BstDEI	CTNAG	8 cut(s)	510, 1220, 1235, 1386, 1667, 1960, 2108, 2126
BstDSI	CCRYGG	1 cut(s)	559
BstENI	CCTNNNNNAGG	1 cut(s)	759
BstF5I	GGATG	5 cut(s)	487, 648, 1236, 1505, 1915
BstFNI	CGCG	1 cut(s)	561
BstKTI	GATC	7 cut(s)	204, 1119, 1404, 1620, 1869, 1976, 2133
BstMAI	GTCTC	4 cut(s)	125, 900, 1899, 2193
BstMBI	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
BstMCI	CGRYCG	1 cut(s)	1585
BstMWI	GCNNNNNNNGC	3 cut(s)	92, 370, 941
BstNI	CCWGG	3 cut(s)	1749, 1854, 2097
BstNSI	RCATGY	5 cut(s)	457, 1473, 1522, 1795, 1894
BstPAI	GACNNNNGTC	1 cut(s)	826
BstSCI	CCNGG	8 cut(s)	5, 315, 410, 650, 1544, 1747, 1852, 2095
BstSFI	CTRYAG	2 cut(s)	759, 1858
BstSLI	GKGCMC	2 cut(s)	453, 853
BstUI	CGCG	1 cut(s)	561
BstV1I	GCAGC	4 cut(s)	1193, 1214, 1485, 1844
BstV2I	GAAGAC	1 cut(s)	2094
BstX2I	RGATCY	2 cut(s)	1401, 2130
BstYI	RGATCY	2 cut(s)	1401, 2130
Bsu36I	CCTNAGG	2 cut(s)	2108, 2126
BsuRI	GGCC	4 cut(s)	86, 309, 350, 578
BtgI	CCRYGG	1 cut(s)	559
BtrI	CACGTC	1 cut(s)	885
BtsCI	GGATG	5 cut(s)	487, 648, 1236, 1505, 1915
BtsI	GCAGTG	1 cut(s)	1261
BtsIMutI	CAGTG	5 cut(s)	343, 538, 986, 1261, 2059
Cac8I	GCNNGC	6 cut(s)	880, 937, 960, 1157, 1471, 1564
CaiI	CAGNNNCTG	2 cut(s)	109, 2133
Cfr13I	GGNCC	5 cut(s)	85, 307, 388, 577, 1044
Cfr42I	CCGCGG	1 cut(s)	562
Csp6I	GTAC	1 cut(s)	681
CviQI	GTAC	1 cut(s)	681
DdeI	CTNAG	8 cut(s)	510, 1220, 1235, 1386, 1667, 1960, 2108, 2126
DpnI	GATC	7 cut(s)	203, 1118, 1403, 1619, 1868, 1975, 2132
DpnII	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
DraI	TTTAAA	1 cut(s)	2224
Eam1104I	CTCTTC	4 cut(s)	335, 1730, 1823, 1964
EarI	CTCTTC	4 cut(s)	335, 1730, 1823, 1964
EciI	GGCGGA	1 cut(s)	367
Ecl136II	GAGCTC	1 cut(s)	1959
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	1961
Eco31I	GGTCTC	1 cut(s)	125
Eco32I	GATATC	1 cut(s)	1441
Eco47I	GGWCC	2 cut(s)	388, 1044
Eco53kI	GAGCTC	1 cut(s)	1959
Eco57I	CTGAAG	3 cut(s)	602, 680, 1821
Eco81I	CCTNAGG	2 cut(s)	2108, 2126
Eco88I	CYCGRG	2 cut(s)	303, 897
EcoICRI	GAGCTC	1 cut(s)	1959
EcoNI	CCTNNNNNAGG	1 cut(s)	759
EcoO109I	RGGNCCY	2 cut(s)	307, 577
EcoRI	GAATTC	1 cut(s)	1871
EcoRII	CCWGG	3 cut(s)	1747, 1852, 2095
EcoRV	GATATC	1 cut(s)	1441
EcoT22I	ATGCAT	2 cut(s)	708, 1377
EcoT38I	GRGCYC	1 cut(s)	1961
FalI	AAGNNNNNCTT	2 cut(s)	1709, 1741
FaqI	GGGAC	3 cut(s)	299, 779, 834
FauI	CCCGC	2 cut(s)	554, 810
FbaI	TGATCA	1 cut(s)	1617
FblI	GTMKAC	1 cut(s)	1709
Fnu4HI	GCNGC	6 cut(s)	559, 1182, 1203, 1285, 1474, 1858
FokI	GGATG	5 cut(s)	494, 655, 1243, 1512, 1902
FriOI	GRGCYC	1 cut(s)	1961
Fsp4HI	GCNGC	6 cut(s)	559, 1182, 1203, 1285, 1474, 1858
FspBI	CTAG	5 cut(s)	678, 735, 744, 1059, 1329
GluI	GCNGC	6 cut(s)	559, 1182, 1203, 1285, 1474, 1858
GsuI	CTGGAG	2 cut(s)	1010, 2079
HaeIII	GGCC	4 cut(s)	86, 309, 350, 578
HapII	CCGG	8 cut(s)	7, 317, 357, 411, 651, 1163, 1546, 1739
HincII	GTYRAC	1 cut(s)	979
HindII	GTYRAC	1 cut(s)	979
HindIII	AAGCTT	1 cut(s)	584
HinfI	GANTC	7 cut(s)	106, 197, 425, 790, 796, 1706, 1831
HpaII	CCGG	8 cut(s)	7, 317, 357, 411, 651, 1163, 1546, 1739
HphI	GGTGA	2 cut(s)	920, 2150
Hpy166II	GTNNAC	9 cut(s)	451, 851, 979, 1427, 1684, 1710, 1797, 1888, 2048
Hpy188III	TCNNGA	6 cut(s)	897, 1222, 1302, 1621, 1876, 2128
Hpy8I	GTNNAC	9 cut(s)	451, 851, 979, 1427, 1684, 1710, 1797, 1888, 2048
Hpy99I	CGWCG	1 cut(s)	889
HpyAV	CCTTC	7 cut(s)	256, 484, 707, 1072, 1192, 2023, 2171
HpyCH4III	ACNGT	5 cut(s)	533, 685, 1484, 1535, 2054
HpyCH4IV	ACGT	2 cut(s)	783, 884
HpyF10VI	GCNNNNNNNGC	3 cut(s)	92, 370, 941
HpyF3I	CTNAG	8 cut(s)	510, 1220, 1235, 1386, 1667, 1960, 2108, 2126
HpySE526I	ACGT	2 cut(s)	783, 884
Ksp22I	TGATCA	1 cut(s)	1617
KspI	CCGCGG	1 cut(s)	562
Kzo9I	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
LguI	GCTCTTC	1 cut(s)	1730
LmnI	GCTCC	5 cut(s)	663, 1096, 1129, 1658, 2032
Lsp1109I	GCAGC	4 cut(s)	1193, 1214, 1485, 1844
LweI	GCATC	5 cut(s)	472, 1101, 1221, 1404, 1633
MaeI	CTAG	5 cut(s)	678, 735, 744, 1059, 1329
MaeII	ACGT	2 cut(s)	783, 884
MaeIII	GTNAC	4 cut(s)	467, 949, 1570, 1693
MalI	GATC	7 cut(s)	203, 1118, 1403, 1619, 1868, 1975, 2132
MbiI	CCGCTC	1 cut(s)	1362
MboI	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
MflI	RGATCY	2 cut(s)	1401, 2130
MhlI	GDGCHC	3 cut(s)	453, 853, 1961
MlyI	GAGTC	2 cut(s)	1715, 1840
MmeI	TCCRAC	4 cut(s)	641, 810, 867, 1844
Mph1103I	ATGCAT	2 cut(s)	708, 1377
MroXI	GAANNNNTTC	1 cut(s)	1724
MseI	TTAA	5 cut(s)	59, 1008, 1512, 2118, 2223
MslI	CAYNNNNRTG	2 cut(s)	804, 1689
MspA1I	CMGCKG	1 cut(s)	561
MspI	CCGG	8 cut(s)	7, 317, 357, 411, 651, 1163, 1546, 1739
MspR9I	CCNGG	8 cut(s)	7, 317, 412, 652, 1546, 1749, 1854, 2097
Mva1269I	GAATGC	2 cut(s)	706, 1354
MvaI	CCWGG	3 cut(s)	1749, 1854, 2097
MvnI	CGCG	1 cut(s)	561
MwoI	GCNNNNNNNGC	3 cut(s)	92, 370, 941
NciI	CCSGG	5 cut(s)	7, 317, 412, 652, 1546
NdeII	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
NlaIV	GGNNCC	2 cut(s)	308, 1131
NmuCI	GTSAC	3 cut(s)	467, 1570, 1693
NsiI	ATGCAT	2 cut(s)	708, 1377
NspI	RCATGY	5 cut(s)	457, 1473, 1522, 1795, 1894
PaeI	GCATGC	1 cut(s)	1473
PaeR7I	CTCGAG	1 cut(s)	897
PceI	AGGCCT	1 cut(s)	350
PciI	ACATGT	1 cut(s)	453
PciSI	GCTCTTC	1 cut(s)	1730
PctI	GAATGC	2 cut(s)	706, 1354
PdmI	GAANNNNTTC	1 cut(s)	1724
PfeI	GAWTC	5 cut(s)	106, 197, 425, 790, 796
PfoI	TCCNGGA	1 cut(s)	315
PkrI	GCNGC	6 cut(s)	560, 1183, 1204, 1286, 1475, 1859
PleI	GAGTC	2 cut(s)	1714, 1839
PpsI	GAGTC	2 cut(s)	1714, 1839
PscI	ACATGT	1 cut(s)	453
PshAI	GACNNNNGTC	1 cut(s)	826
Psp124BI	GAGCTC	1 cut(s)	1961
Psp6I	CCWGG	3 cut(s)	1747, 1852, 2095
PspGI	CCWGG	3 cut(s)	1747, 1852, 2095
PspN4I	GGNNCC	2 cut(s)	308, 1131
PspPI	GGNCC	5 cut(s)	85, 307, 388, 577, 1044
PstI	CTGCAG	1 cut(s)	1862
PstNI	CAGNNNCTG	2 cut(s)	109, 2133
PsuI	RGATCY	2 cut(s)	1401, 2130
RsaI	GTAC	1 cut(s)	682
RsaNI	GTAC	1 cut(s)	681
RseI	CAYNNNNRTG	2 cut(s)	804, 1689
SacI	GAGCTC	1 cut(s)	1961
SacII	CCGCGG	1 cut(s)	562
SapI	GCTCTTC	1 cut(s)	1730
SaqAI	TTAA	5 cut(s)	59, 1008, 1512, 2118, 2223
SatI	GCNGC	6 cut(s)	559, 1182, 1203, 1285, 1474, 1858
Sau3AI	GATC	7 cut(s)	201, 1116, 1401, 1617, 1866, 1973, 2130
Sau96I	GGNCC	5 cut(s)	85, 307, 388, 577, 1044
SchI	GAGTC	2 cut(s)	1715, 1840
ScrFI	CCNGG	8 cut(s)	7, 317, 412, 652, 1546, 1749, 1854, 2097
SduI	GDGCHC	3 cut(s)	453, 853, 1961
SfaNI	GCATC	5 cut(s)	472, 1101, 1221, 1404, 1633
SfcI	CTRYAG	2 cut(s)	759, 1858
Sfr274I	CTCGAG	1 cut(s)	897
Sfr303I	CCGCGG	1 cut(s)	562
SgrBI	CCGCGG	1 cut(s)	562
SinI	GGWCC	2 cut(s)	388, 1044
SlaI	CTCGAG	1 cut(s)	897
SmiMI	CAYNNNNRTG	2 cut(s)	804, 1689
SmlI	CTYRAG	1 cut(s)	897
SmoI	CTYRAG	1 cut(s)	897
SphI	GCATGC	1 cut(s)	1473
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	6 cut(s)	378, 559, 561, 817, 1284, 1362
SspI	AATATT	3 cut(s)	1033, 1177, 1774
SspMI	CTAG	5 cut(s)	678, 735, 744, 1059, 1329
SstI	GAGCTC	1 cut(s)	1961
StuI	AGGCCT	1 cut(s)	350
StyD4I	CCNGG	8 cut(s)	5, 315, 410, 650, 1544, 1747, 1852, 2095
TaaI	ACNGT	5 cut(s)	533, 685, 1484, 1535, 2054
TaiI	ACGT	2 cut(s)	786, 887
TaqI	TCGA	8 cut(s)	195, 200, 423, 858, 898, 1119, 1303, 1869
TaqII	GACCGA	2 cut(s)	486, 1571
TatI	WGTACW	1 cut(s)	680
TauI	GCSGC	2 cut(s)	561, 1287
TfiI	GAWTC	5 cut(s)	106, 197, 425, 790, 796
Tru1I	TTAA	5 cut(s)	59, 1008, 1512, 2118, 2223
Tru9I	TTAA	5 cut(s)	59, 1008, 1512, 2118, 2223
TscAI	CASTG	5 cut(s)	343, 538, 993, 1261, 2059
TseFI	GTSAC	3 cut(s)	467, 1570, 1693
TseI	GCWGC	4 cut(s)	1181, 1202, 1473, 1857
Tsp45I	GTSAC	3 cut(s)	467, 1570, 1693
TspDTI	ATGAA	7 cut(s)	230, 788, 1841, 1940, 1982, 2152, 2204
TspGWI	ACGGA	1 cut(s)	1952
TspRI	CASTG	5 cut(s)	343, 538, 993, 1261, 2059
VneI	GTGCAC	2 cut(s)	449, 849
VpaK11BI	GGWCC	2 cut(s)	388, 1044
XagI	CCTNNNNNAGG	1 cut(s)	759
XapI	RAATTY	3 cut(s)	1333, 1871, 2159
XceI	RCATGY	5 cut(s)	457, 1473, 1522, 1795, 1894
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1333
XhoI	CTCGAG	1 cut(s)	897
XmiI	GTMKAC	1 cut(s)	1709
XmnI	GAANNNNTTC	1 cut(s)	1724
XspI	CTAG	5 cut(s)	678, 735, 744, 1059, 1329
Zsp2I	ATGCAT	2 cut(s)	708, 1377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.