Rorug06G0322800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008188
Physical Location & Seq
Forward (+)
47437131 .. 47440728
3598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0322800.1

Sequence Viewer

Length: 1032 bp
ATGGGCAGCAGCAGCAGCGTCGAAACGGCGTCGTCGTCAATGAGGGAGTTTCTGCGGCAGGCCGGCGGCGTCGCCGTCGTCGACGGTGGTCTGGCGACCGAGCTCGAGCGGCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTCACTTCCTCTCACCTCATTCGCGCCGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACAGCTTCTTATCAGGCAACTATTCAAGGTTTTGAGGCCAAAGGGTTTTCGACTGAGGAGAGTGAATCATTGCTTAGGAAGAGTGTGGAGATTGCGCGTGGGGCGCGAGATATTTATTACGAAAGGTGTAGTGAGGGGTCTTGTAATGATAGTGATGGTAGAGTTCTCAAACGTCGGCCCATCATAGTTGCAGCATCTGTGGGGAGCTATGGGGCTTATTTGGCTGATGGGTCTGAGTACAGCGGTGATTATGGTGAGGCTATGACGCTAGGAGCATTAAAAGAGTTTCATCGTAGAAGAGTCCAAGTCCTAACAGAAGCAGGTCCTGACCTACTTGCATTTGAAACTATTCCAAATAAGCTGGAAGCCCAGGCTTTTGCTGAGCTTTTGGAAGAAGAAGACATAAAGCTTCCTGCATGGTTTTGTTTCAACTCTAAAGATGGAATCAATGTTGTTAGTGGTGATTCTTTCCTTGAATGTGCCTCGGTTGCTGAATCCTGTAAGAAAGTTGTTGCTGTGGGGATTAACTGTACTCCTCCTAGATTTATTCATGGACTGATCACAACAATTAAAAAGGTGGCTACAAAACCAATAGTTATATACCCAAATAGTGGCGAAAGTTATGATGCTGATCGAAAGGAGTGGCAAAATACTGGGGTTTCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTGCCTTGTGGGAGGTTGTTGCAGAACAACTCCAAATACTATCAGGGCCATCTACAAAACCCTTCCCAATCGGTCAACATCTCCACCTGAACAGTAA

Protein Analysis

343

Amino Acids

37.21

Weight (kDa)

5.05

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 26 - 332 1.3e-80 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 836
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 180, 322, 331
AciI CCGC 4 cut(s) 55, 66, 109, 468
AclWI GGATC 1 cut(s) 125
AcyI GRCGYC 2 cut(s) 29, 69
AfaI GTAC 2 cut(s) 464, 757
AfiI CCNNNNNNNGG 1 cut(s) 836
AgsI TTSAA 5 cut(s) 203, 251, 569, 655, 701
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 7 cut(s) 103, 206, 230, 432, 586, 610, 634
AluI AGCT 7 cut(s) 103, 206, 230, 432, 586, 610, 634
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 2 cut(s) 422, 551
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 4 cut(s) 60, 261, 401, 981
ApeKI GCWGC 5 cut(s) 6, 9, 12, 15, 416
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 5 cut(s) 119, 146, 182, 322, 331
AspS9I GGNCC 3 cut(s) 402, 548, 981
AsuHPI GGTGA 4 cut(s) 161, 482, 491, 698
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 5 cut(s) 18, 21, 24, 27, 428
BccI CCATC 5 cut(s) 374, 413, 446, 659, 992
BceAI ACGGC 4 cut(s) 42, 59, 130, 167
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 596
BclI TGATCA 1 cut(s) 783
BfaI CTAG 2 cut(s) 494, 765
BfoI RGCGCY 2 cut(s) 120, 147
BfuAI ACCTGC 1 cut(s) 536
BisI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 8 cut(s) 8, 11, 14, 17, 57, 68, 111, 418
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 402, 548, 981
BmrFI CCNGG 1 cut(s) 596
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 2 cut(s) 428, 841
BmuI ACTGGG 1 cut(s) 888
BoxI GACNNNNGTC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 855
BsaHI GRCGYC 2 cut(s) 29, 69
BsaJI CCNNGG 2 cut(s) 594, 708
Bsc4I CCNNNNNNNGG 1 cut(s) 836
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 293
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 596
BseDI CCNNGG 2 cut(s) 594, 708
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 1 cut(s) 836
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 3 cut(s) 270, 450, 597
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 3 cut(s) 146, 296, 750
BseXI GCAGC 5 cut(s) 18, 21, 24, 27, 428
Bsh1236I CGCG 3 cut(s) 180, 322, 331
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 4 cut(s) 62, 263, 403, 983
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 836
BsnI GGCC 4 cut(s) 62, 263, 403, 983
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 3 cut(s) 130, 783, 856
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 4 cut(s) 55, 66, 109, 468
BspANI GGCC 4 cut(s) 62, 263, 403, 983
BspCNI CTCAG 3 cut(s) 271, 451, 598
BspFNI CGCG 3 cut(s) 180, 322, 331
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 883
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 594, 708
BssMI GATC 3 cut(s) 130, 783, 856
BssNI GRCGYC 2 cut(s) 29, 69
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 86, 755, 1029
Bst6I CTCTTC 2 cut(s) 299, 517
BstACI GRCGYC 2 cut(s) 29, 69
BstC8I GCNNGC 3 cut(s) 60, 64, 937
BstDEI CTNAG 4 cut(s) 279, 299, 459, 606
BstFNI CGCG 3 cut(s) 180, 322, 331
BstH2I RGCGCY 2 cut(s) 120, 147
BstHHI GCGC 5 cut(s) 119, 146, 182, 322, 331
BstKTI GATC 3 cut(s) 133, 786, 859
BstMBI GATC 3 cut(s) 130, 783, 856
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
BstNI CCWGG 1 cut(s) 596
BstPAI GACNNNNGTC 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 594
BstUI CGCG 3 cut(s) 180, 322, 331
BstV1I GCAGC 5 cut(s) 18, 21, 24, 27, 428
BstV2I GAAGAC 1 cut(s) 630
BsuRI GGCC 4 cut(s) 62, 263, 403, 983
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 3 cut(s) 60, 64, 937
CaiI CAGNNNCTG 2 cut(s) 422, 551
CfoI GCGC 5 cut(s) 119, 146, 182, 322, 331
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 402, 548, 981
CseI GACGC 4 cut(s) 7, 18, 58, 499
Csp6I GTAC 2 cut(s) 463, 756
CviAII CATG 2 cut(s) 642, 776
CviQI GTAC 2 cut(s) 463, 756
DdeI CTNAG 4 cut(s) 279, 299, 459, 606
DpnI GATC 3 cut(s) 132, 785, 858
DpnII GATC 3 cut(s) 130, 783, 856
Eam1104I CTCTTC 2 cut(s) 299, 517
EarI CTCTTC 2 cut(s) 299, 517
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 548
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 594
EcoRV GATATC 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 2 cut(s) 645, 779
FatI CATG 2 cut(s) 641, 775
FauNDI CATATG 1 cut(s) 907
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
FspBI CTAG 2 cut(s) 494, 765
GlaI GCGC 5 cut(s) 118, 145, 181, 321, 330
GluI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
GsuI CTGGAG 1 cut(s) 160
HaeII RGCGCY 2 cut(s) 120, 147
HaeIII GGCC 4 cut(s) 62, 263, 403, 983
HapII CCGG 1 cut(s) 63
HgaI GACGC 4 cut(s) 7, 18, 58, 499
HhaI GCGC 5 cut(s) 119, 146, 182, 322, 331
Hin1I GRCGYC 2 cut(s) 29, 69
Hin1II CATG 2 cut(s) 645, 779
Hin6I GCGC 5 cut(s) 117, 144, 180, 320, 329
HinP1I GCGC 5 cut(s) 117, 144, 180, 320, 329
HincII GTYRAC 2 cut(s) 82, 1011
HindII GTYRAC 2 cut(s) 82, 1011
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 290, 525, 669, 689, 719
HpaII CCGG 1 cut(s) 63
HphI GGTGA 4 cut(s) 161, 482, 491, 698
Hpy166II GTNNAC 3 cut(s) 82, 913, 1011
Hpy188I TCNGA 2 cut(s) 460, 889
Hpy188III TCNNGA 4 cut(s) 139, 191, 200, 551
Hpy8I GTNNAC 3 cut(s) 82, 913, 1011
Hpy99I CGWCG 8 cut(s) 23, 34, 37, 74, 80, 83, 86, 402
HpyAV CCTTC 1 cut(s) 1007
HpyCH4III ACNGT 3 cut(s) 86, 755, 1029
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 4 cut(s) 416, 563, 641, 957
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
HpyF3I CTNAG 4 cut(s) 279, 299, 459, 606
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 2 cut(s) 29, 69
Hsp92II CATG 2 cut(s) 645, 779
HspAI GCGC 5 cut(s) 117, 144, 180, 320, 329
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 3 cut(s) 130, 783, 856
LmnI GCTCC 3 cut(s) 141, 429, 497
Lsp1109I GCAGC 5 cut(s) 18, 21, 24, 27, 428
LweI GCATC 2 cut(s) 428, 841
MaeI CTAG 2 cut(s) 494, 765
MaeII ACGT 1 cut(s) 397
MalI GATC 3 cut(s) 132, 785, 858
MbiI CCGCTC 1 cut(s) 109
MboI GATC 3 cut(s) 130, 783, 856
MboII GAAGA 6 cut(s) 316, 534, 629, 632, 635, 905
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 1 cut(s) 792
MlyI GAGTC 1 cut(s) 534
MroNI GCCGGC 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 3 cut(s) 503, 750, 795
MspA1I CMGCKG 1 cut(s) 468
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 596
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 3 cut(s) 180, 322, 331
MwoI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
NaeI GCCGGC 1 cut(s) 64
NdeI CATATG 1 cut(s) 907
NdeII GATC 3 cut(s) 130, 783, 856
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 2 cut(s) 645, 779
PaeR7I CTCGAG 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 78
PdiI GCCGGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 4 cut(s) 290, 669, 689, 719
PflMI CCANNNNNTGG 1 cut(s) 836
PkrI GCNGC 8 cut(s) 8, 11, 14, 17, 57, 68, 111, 418
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
PpuMI RGGWCCY 1 cut(s) 548
PshAI GACNNNNGTC 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 594
PspGI CCWGG 1 cut(s) 594
PspPI GGNCC 3 cut(s) 402, 548, 981
PspPPI RGGWCCY 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 104
PstNI CAGNNNCTG 2 cut(s) 422, 551
RsaI GTAC 2 cut(s) 464, 757
RsaNI GTAC 2 cut(s) 463, 756
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 503, 750, 795
SatI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
Sau3AI GATC 3 cut(s) 130, 783, 856
Sau96I GGNCC 3 cut(s) 402, 548, 981
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 2 cut(s) 428, 841
Sfr274I CTCGAG 1 cut(s) 104
SgrDI CGTCGACG 1 cut(s) 80
SinI GGWCC 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 1 cut(s) 792
SsiI CCGC 4 cut(s) 55, 66, 109, 468
SspMI CTAG 2 cut(s) 494, 765
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 86, 755, 1029
TaiI ACGT 1 cut(s) 400
TaqI TCGA 5 cut(s) 21, 81, 105, 275, 859
TaqII GACCGA 2 cut(s) 113, 996
TasI AATT 1 cut(s) 792
TatI WGTACW 2 cut(s) 462, 755
TauI GCSGC 3 cut(s) 58, 69, 112
TfiI GAWTC 4 cut(s) 290, 669, 689, 719
Tru1I TTAA 3 cut(s) 503, 750, 795
Tru9I TTAA 3 cut(s) 503, 750, 795
TseI GCWGC 5 cut(s) 6, 9, 12, 15, 416
TspDTI ATGAA 5 cut(s) 176, 503, 764, 894, 906
Van91I CCANNNNNTGG 1 cut(s) 836
VpaK11BI GGWCC 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 2 cut(s) 494, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.