Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0008188

Physical Location & Seq

Genomic Coordinates Forward (+)

61420011 .. 61423460

Gene Structure 3450 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG435900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGGACTCTGTTTCACAAAATCCCACACCCATGATATCCCAATCTCTTCATCATCAGAATCACCACCTCACCACTTCCAACAAACACAACCCAACAAAAAACCCGACCCGTTTCACCAGCCAGCTCCACCCAAACCCAGCCCCTACTCCGGAGGCTCTCTCGGCAAGAGCCCCTCGTCCTCCCAAATCGGACCAATTCTGGGCAAGCCTTACGCCGACGTAAATTCCGTTTATACCCTCCAGAAGGAGCTGGGCCGCGGCCAGTTCGGCGTCACGCGTCTCTGCACGGAGAAATCCACTGGCCGAAAATACGCTTGCAAGTCCATATCGTGCAAAAAACTCGTGACGAATAAGGACATTGAGGACTTGCGGAGAGAGGTTCTGATTCTGGAGCACTTAACAGGCCAGCCCAATATAGTTGAATTCAAAGGTGCTTTTGAGGACCGCCAGAATCTGCATTTGATTATGGAGCTCTGCACCGGCGGCGAGCTTTTTGATCGGATTATTGCCAAGGGAAGCTACACCGAGCGTGAAGCGGCCAAGATTTTCCGGCAGATTGTGAATGTGGTGCATGTGTGTCACTTTATGGGAGTGATGCATAGGGATTTGAAGCCTGAGAATTTCTTGCTTGTAAGTAAGGATGAGGATGCACCCATAAAGGCCATTGATTTTGGTCTCTCTGTCTTCATCGAGCAAGAGACCGAGAAAGGGATATTTGACTCAATTATAGAAGGAAAACTTGACTTGCATAGTGCGCCATGGCCTTCTATACCTGATGCGGCAAAGGATCTCATCAGGAAAATGTTAACAATGGACCCTAAGAAAAGGATTACAGCTGCTGAAGTTCTTGAGCATCAATGGATGAAGAAAGATGGTGAAGCATCTGACAAACCTATTGATAGTGCTGTGCTGATTAGGATGAAACAGTTCAGAGCAATGAACAAGTTGAAGAAACTTGCTCTAAAGGTCATAGCAGAAACGCTTTCTGAAGAAGAGATTCATGGCCTGAAACAAATGTTCAACAACATTGACACAGATGGTAGTGGTAATATCACAGTTGAAGAACTAAGAACTGGATTGTCAAGGCTGGGATCTAAGCTTACAGAAGCAGAAATAAAGCAGCTGATGGATGCTGCTGACATAGACAATAATGGGACTATTGATTACACTGAATTCATTACTGCAACCATGCATCGACACAAACTGGAGAAGGAAGAAAACTTGCACAAGGCTTTCCTTTACTTTGACAAGGATCAGAGCGGGTTTATCACAAGAGATGAGCTAAGACAAGCAATGACTGAATATGGGATGGGGGATGAGGCTACGATAGATGAAGTCCTTGAAGATGTGGATACTGATAAAGATGGGAGAATCAATTATGAGGAGTTCGTAGCCATGATGACACGGGGAGTTGCAGATTACGATGATAAACCAGATAGGGTGATTGTTCTACCTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0098542 GO:0140096 GO:1901000 GO:1901002 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 Pkinase

Protein Analysis

486

Amino Acids

54.78

Weight (kDa)

5.61

Isoelectric Point (pI)

44.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	78 - 235	5.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	79 - 234	5.6e-18	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	241 - 288	5.5e-08	Protein kinase domain
EF-hand_7	PF13499	336 - 396	1.6e-14	EF-hand domain pair
EF-hand_1	PF00036	336 - 362	4.7e-06	EF hand domain
EF-hand_6	PF13405	336 - 364	8.8e-06	EF-hand domain
EF-hand_5	PF13202	336 - 358	7.1e-06	EF hand
EF-hand_8	PF13833	348 - 395	8.8e-08	EF-hand domain pair
EF-hand_7	PF13499	405 - 468	3e-16	EF-hand domain pair
EF-hand_1	PF00036	408 - 434	1e-06	EF hand domain
EF-hand_6	PF13405	408 - 436	2.9e-07	EF-hand domain
EF-hand_8	PF13833	420 - 469	4.1e-06	EF-hand domain pair
EF-hand_1	PF00036	443 - 469	1.9e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0008188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76040 AT1G76040 AT1G76040 AT1G76040
fragaria_vesca	FvH4_2g38010 FvH4_2g38010
malus_domestica	MD15G1007200.v1.1
prunus_persica	Prupe.1G360100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0303601
rosa_laevigata	RLG00000011069 RLG00000011734
rosa_multiflora	Rmu_ssc0000114.1_g000023
rosa_roxburghii	Rroxscaffold_7G00164590
rosa_rugosa	Rorug06G0257300 Rorug06G0322800
rosa_samantha	Rh6AG437100 Rh6BG377600 Rh6BG377700 Rh6BG466100 Rh6CG449200 Rh6DG370300 Rh6DG435900
rosa_wichuraiana	Rw6G037840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1260
AccII	CGCG	2 cut(s)	258, 277
AccIII	TCCGGA	1 cut(s)	149
AciI	CCGC	8 cut(s)	256, 258, 370, 445, 483, 536, 779, 1260
AclWI	GGATC	3 cut(s)	795, 1099, 1260
AcoI	YGGCCR	3 cut(s)	259, 301, 537
AcsI	RAATTY	4 cut(s)	223, 422, 619, 1172
AcuI	CTGAAG	2 cut(s)	861, 1008
AcyI	GRCGYC	1 cut(s)	270
AfiI	CCNNNNNNNGG	4 cut(s)	149, 200, 244, 708
AflIII	ACRYGT	1 cut(s)	275
AgsI	TTSAA	7 cut(s)	422, 427, 610, 949, 1021, 1061, 1343
AluBI	AGCT	9 cut(s)	125, 250, 472, 490, 519, 836, 1099, 1123, 1282
AluI	AGCT	9 cut(s)	125, 250, 472, 490, 519, 836, 1099, 1123, 1282
Alw21I	GWGCWC	2 cut(s)	396, 474
Alw26I	GTCTC	3 cut(s)	284, 680, 692
AlwI	GGATC	3 cut(s)	795, 1099, 1260
AlwNI	CAGNNNCTG	2 cut(s)	454, 839
Aor13HI	TCCGGA	1 cut(s)	149
AoxI	GGCC	8 cut(s)	253, 259, 301, 403, 537, 660, 761, 1003
ApeKI	GCWGC	3 cut(s)	836, 1120, 1133
ApoI	RAATTY	4 cut(s)	223, 422, 619, 1172
Asp700I	GAANNNNTTC	2 cut(s)	926, 996
AspLEI	GCGC	1 cut(s)	757
AspS9I	GGNCC	4 cut(s)	191, 253, 442, 814
AsuHPI	GGTGA	5 cut(s)	54, 62, 107, 887, 1453
AvaII	GGWCC	3 cut(s)	191, 442, 814
BanII	GRGCYC	2 cut(s)	173, 474
BauI	CACGAG	1 cut(s)	341
BbsI	GAAGAC	1 cut(s)	676
Bbv12I	GWGCWC	2 cut(s)	396, 474
BbvI	GCAGC	3 cut(s)	823, 1120, 1132
BccI	CCATC	5 cut(s)	866, 1031, 1120, 1303, 1358
BcgI	CGANNNNNNTGC	2 cut(s)	322, 356
BciVI	GTATCC	1 cut(s)	1345
BcoDI	GTCTC	3 cut(s)	284, 680, 692
BfuI	GTATCC	1 cut(s)	1345
BglI	GCCNNNNNGGC	1 cut(s)	267
BisI	GCNGC	8 cut(s)	256, 259, 484, 537, 780, 837, 1121, 1134
BlsI	GCNGC	8 cut(s)	257, 260, 485, 538, 781, 838, 1122, 1135
Bme18I	GGWCC	3 cut(s)	191, 442, 814
BmgT120I	GGNCC	4 cut(s)	191, 253, 442, 814
BmiI	GGNNCC	1 cut(s)	816
BmsI	GCATC	7 cut(s)	585, 637, 766, 862, 890, 1120, 1201
BpiI	GAAGAC	1 cut(s)	676
BplI	GAGNNNNNCTC	2 cut(s)	144, 176
BpmI	CTGGAG	3 cut(s)	224, 410, 1226
BpuEI	CTTGAG	1 cut(s)	869
BsaHI	GRCGYC	1 cut(s)	270
BsaI	GGTCTC	2 cut(s)	680, 692
BsaJI	CCNNGG	3 cut(s)	256, 510, 758
BsaWI	WCCGGW	1 cut(s)	149
Bsc4I	CCNNNNNNNGG	4 cut(s)	149, 200, 244, 708
Bse118I	RCCGGY	1 cut(s)	479
Bse1I	ACTGG	4 cut(s)	262, 304, 1078, 1209
Bse3DI	GCAATG	2 cut(s)	942, 1299
BseAI	TCCGGA	1 cut(s)	149
BseDI	CCNNGG	3 cut(s)	256, 510, 758
BseGI	GGATG	7 cut(s)	646, 652, 867, 924, 1135, 1314, 1321
BseLI	CCNNNNNNNGG	4 cut(s)	149, 200, 244, 708
BseMI	GCAATG	2 cut(s)	942, 1299
BseMII	CTCAG	1 cut(s)	606
BseNI	ACTGG	4 cut(s)	262, 304, 1078, 1209
BseRI	GAGGAG	1 cut(s)	1397
BseXI	GCAGC	3 cut(s)	823, 1120, 1132
BseYI	CCCAGC	3 cut(s)	137, 250, 1087
BsgI	GTGCAG	2 cut(s)	268, 460
Bsh1236I	CGCG	2 cut(s)	258, 277
BshFI	GGCC	8 cut(s)	255, 261, 303, 405, 539, 662, 763, 1005
BsiHKAI	GWGCWC	2 cut(s)	396, 474
BsiSI	CCGG	3 cut(s)	150, 480, 550
BslFI	GGGAC	2 cut(s)	18, 1168
BslI	CCNNNNNNNGG	4 cut(s)	149, 200, 244, 708
BsmAI	GTCTC	3 cut(s)	284, 680, 692
BsmBI	CGTCTC	1 cut(s)	284
BsmFI	GGGAC	2 cut(s)	18, 1168
BsnI	GGCC	8 cut(s)	255, 261, 303, 405, 539, 662, 763, 1005
Bso31I	GGTCTC	2 cut(s)	680, 692
Bsp1286I	GDGCHC	3 cut(s)	173, 396, 474
Bsp13I	TCCGGA	1 cut(s)	149
Bsp143I	GATC	4 cut(s)	496, 787, 1091, 1252
Bsp19I	CCATGG	1 cut(s)	758
BspACI	CCGC	8 cut(s)	256, 258, 370, 445, 483, 536, 779, 1260
BspANI	GGCC	8 cut(s)	255, 261, 303, 405, 539, 662, 763, 1005
BspCNI	CTCAG	1 cut(s)	607
BspEI	TCCGGA	1 cut(s)	149
BspFNI	CGCG	2 cut(s)	258, 277
BspLI	GGNNCC	1 cut(s)	816
BspPI	GGATC	3 cut(s)	795, 1099, 1260
BspTNI	GGTCTC	2 cut(s)	680, 692
BsrBI	CCGCTC	1 cut(s)	1260
BsrDI	GCAATG	2 cut(s)	942, 1299
BsrFI	RCCGGY	1 cut(s)	479
BsrI	ACTGG	4 cut(s)	262, 304, 1078, 1209
BssAI	RCCGGY	1 cut(s)	479
BssECI	CCNNGG	3 cut(s)	256, 510, 758
BssMI	GATC	4 cut(s)	496, 787, 1091, 1252
BssNI	GRCGYC	1 cut(s)	270
BssSI	CACGAG	1 cut(s)	341
BssT1I	CCWWGG	2 cut(s)	510, 758
Bst2BI	CACGAG	1 cut(s)	341
Bst4CI	ACNGT	2 cut(s)	927, 1057
Bst6I	CTCTTC	2 cut(s)	52, 987
BstACI	GRCGYC	1 cut(s)	270
BstC8I	GCNNGC	5 cut(s)	123, 206, 316, 407, 488
BstDEI	CTNAG	5 cut(s)	615, 819, 1067, 1095, 1283
BstDSI	CCRYGG	2 cut(s)	256, 758
BstENI	CCTNNNNNAGG	1 cut(s)	242
BstF5I	GGATG	7 cut(s)	646, 652, 867, 924, 1135, 1314, 1321
BstFNI	CGCG	2 cut(s)	258, 277
BstHHI	GCGC	1 cut(s)	757
BstKTI	GATC	4 cut(s)	499, 790, 1094, 1255
BstMAI	GTCTC	3 cut(s)	284, 680, 692
BstMBI	GATC	4 cut(s)	496, 787, 1091, 1252
BstMWI	GCNNNNNNNGC	4 cut(s)	162, 267, 483, 754
BstNSI	RCATGY	1 cut(s)	575
BstUI	CGCG	2 cut(s)	258, 277
BstV1I	GCAGC	3 cut(s)	823, 1120, 1132
BstV2I	GAAGAC	1 cut(s)	676
BstX2I	RGATCY	2 cut(s)	787, 1091
BstYI	RGATCY	2 cut(s)	787, 1091
BsuI	GTATCC	1 cut(s)	1345
BsuRI	GGCC	8 cut(s)	255, 261, 303, 405, 539, 662, 763, 1005
BtgI	CCRYGG	2 cut(s)	256, 758
BtsCI	GGATG	7 cut(s)	646, 652, 867, 924, 1135, 1314, 1321
BtsIMutI	CAGTG	2 cut(s)	297, 1167
Cac8I	GCNNGC	5 cut(s)	123, 206, 316, 407, 488
CaiI	CAGNNNCTG	2 cut(s)	454, 839
CfoI	GCGC	1 cut(s)	757
Cfr10I	RCCGGY	1 cut(s)	479
Cfr13I	GGNCC	4 cut(s)	191, 253, 442, 814
Cfr42I	CCGCGG	1 cut(s)	259
CseI	GACGC	2 cut(s)	259, 266
CviAII	CATG	6 cut(s)	32, 572, 759, 1001, 1189, 1396
DdeI	CTNAG	5 cut(s)	615, 819, 1067, 1095, 1283
DpnI	GATC	4 cut(s)	498, 789, 1093, 1254
DpnII	GATC	4 cut(s)	496, 787, 1091, 1252
EaeI	YGGCCR	3 cut(s)	259, 301, 537
Eam1104I	CTCTTC	2 cut(s)	52, 987
EarI	CTCTTC	2 cut(s)	52, 987
Ecl136II	GAGCTC	1 cut(s)	472
Eco130I	CCWWGG	2 cut(s)	510, 758
Eco24I	GRGCYC	2 cut(s)	173, 474
Eco31I	GGTCTC	2 cut(s)	680, 692
Eco32I	GATATC	1 cut(s)	37
Eco47I	GGWCC	3 cut(s)	191, 442, 814
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	2 cut(s)	861, 1008
EcoICRI	GAGCTC	1 cut(s)	472
EcoNI	CCTNNNNNAGG	1 cut(s)	242
EcoRI	GAATTC	2 cut(s)	422, 1172
EcoRV	GATATC	1 cut(s)	37
EcoT14I	CCWWGG	2 cut(s)	510, 758
EcoT22I	ATGCAT	2 cut(s)	600, 1194
EcoT38I	GRGCYC	2 cut(s)	173, 474
ErhI	CCWWGG	2 cut(s)	510, 758
Esp3I	CGTCTC	1 cut(s)	284
FaeI	CATG	6 cut(s)	35, 575, 762, 1004, 1192, 1399
FalI	AAGNNNNNCTT	2 cut(s)	723, 755
FaqI	GGGAC	2 cut(s)	18, 1168
FatI	CATG	6 cut(s)	31, 571, 758, 1000, 1188, 1395
FauI	CCCGC	1 cut(s)	1253
Fnu4HI	GCNGC	8 cut(s)	256, 259, 484, 537, 780, 837, 1121, 1134
FokI	GGATG	7 cut(s)	653, 659, 874, 931, 1142, 1321, 1328
FriOI	GRGCYC	2 cut(s)	173, 474
Fsp4HI	GCNGC	8 cut(s)	256, 259, 484, 537, 780, 837, 1121, 1134
GlaI	GCGC	1 cut(s)	756
GluI	GCNGC	8 cut(s)	256, 259, 484, 537, 780, 837, 1121, 1134
GsaI	CCCAGC	3 cut(s)	141, 254, 1091
GsuI	CTGGAG	3 cut(s)	224, 410, 1226
HaeIII	GGCC	8 cut(s)	255, 261, 303, 405, 539, 662, 763, 1005
HapII	CCGG	3 cut(s)	150, 480, 550
HgaI	GACGC	2 cut(s)	259, 266
HhaI	GCGC	1 cut(s)	757
Hin1I	GRCGYC	1 cut(s)	270
Hin1II	CATG	6 cut(s)	35, 575, 762, 1004, 1192, 1399
Hin6I	GCGC	1 cut(s)	755
HinP1I	GCGC	1 cut(s)	755
HincII	GTYRAC	1 cut(s)	807
HindII	GTYRAC	1 cut(s)	807
HindIII	AAGCTT	1 cut(s)	1097
HinfI	GANTC	7 cut(s)	6, 59, 385, 451, 719, 997, 1371
HpaI	GTTAAC	1 cut(s)	807
HpaII	CCGG	3 cut(s)	150, 480, 550
HphI	GGTGA	5 cut(s)	54, 62, 107, 887, 1453
Hpy166II	GTNNAC	1 cut(s)	807
Hpy188I	TCNGA	8 cut(s)	58, 191, 384, 501, 886, 932, 988, 1257
Hpy188III	TCNNGA	6 cut(s)	150, 241, 343, 389, 796, 848
Hpy8I	GTNNAC	1 cut(s)	807
Hpy99I	CGWCG	1 cut(s)	221
HpyAV	CCTTC	4 cut(s)	238, 725, 774, 1204
HpyCH4III	ACNGT	2 cut(s)	927, 1057
HpyCH4IV	ACGT	1 cut(s)	219
HpyF10VI	GCNNNNNNNGC	4 cut(s)	162, 267, 483, 754
HpyF3I	CTNAG	5 cut(s)	615, 819, 1067, 1095, 1283
HpySE526I	ACGT	1 cut(s)	219
Hsp92I	GRCGYC	1 cut(s)	270
Hsp92II	CATG	6 cut(s)	35, 575, 762, 1004, 1192, 1399
HspAI	GCGC	1 cut(s)	755
Kpn2I	TCCGGA	1 cut(s)	149
KspAI	GTTAAC	1 cut(s)	807
KspI	CCGCGG	1 cut(s)	259
Kzo9I	GATC	4 cut(s)	496, 787, 1091, 1252
LmnI	GCTCC	4 cut(s)	130, 247, 391, 469
Lsp1109I	GCAGC	3 cut(s)	823, 1120, 1132
LweI	GCATC	7 cut(s)	585, 637, 766, 862, 890, 1120, 1201
MaeII	ACGT	1 cut(s)	219
MaeIII	GTNAC	3 cut(s)	271, 343, 578
MalI	GATC	4 cut(s)	498, 789, 1093, 1254
MbiI	CCGCTC	1 cut(s)	1260
MboI	GATC	4 cut(s)	496, 787, 1091, 1252
MboII	GAAGA	9 cut(s)	39, 676, 877, 961, 1001, 1004, 1073, 1226, 1355
MflI	RGATCY	2 cut(s)	787, 1091
MhlI	GDGCHC	3 cut(s)	173, 396, 474
MluCI	AATT	7 cut(s)	195, 223, 422, 619, 723, 1172, 1375
MluI	ACGCGT	1 cut(s)	275
MlyI	GAGTC	1 cut(s)	713
MmeI	TCCRAC	1 cut(s)	103
Mph1103I	ATGCAT	2 cut(s)	600, 1194
MroI	TCCGGA	1 cut(s)	149
MroXI	GAANNNNTTC	2 cut(s)	926, 996
MseI	TTAA	2 cut(s)	398, 806
MslI	CAYNNNNRTG	1 cut(s)	30
MspA1I	CMGCKG	3 cut(s)	258, 836, 1123
MspI	CCGG	3 cut(s)	150, 480, 550
MvnI	CGCG	2 cut(s)	258, 277
MwoI	GCNNNNNNNGC	4 cut(s)	162, 267, 483, 754
NcoI	CCATGG	1 cut(s)	758
NdeII	GATC	4 cut(s)	496, 787, 1091, 1252
NlaIII	CATG	6 cut(s)	35, 575, 762, 1004, 1192, 1399
NlaIV	GGNNCC	1 cut(s)	816
NmeAIII	GCCGAG	1 cut(s)	141
NmuCI	GTSAC	3 cut(s)	271, 343, 578
NsiI	ATGCAT	2 cut(s)	600, 1194
NspI	RCATGY	1 cut(s)	575
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PdmI	GAANNNNTTC	2 cut(s)	926, 996
PfeI	GAWTC	5 cut(s)	59, 385, 451, 997, 1371
PkrI	GCNGC	8 cut(s)	257, 260, 485, 538, 781, 838, 1122, 1135
PleI	GAGTC	1 cut(s)	713
PpsI	GAGTC	1 cut(s)	713
Psp124BI	GAGCTC	1 cut(s)	474
PspFI	CCCAGC	3 cut(s)	137, 250, 1087
PspN4I	GGNNCC	1 cut(s)	816
PspPI	GGNCC	4 cut(s)	191, 253, 442, 814
PstNI	CAGNNNCTG	2 cut(s)	454, 839
PsuI	RGATCY	2 cut(s)	787, 1091
PvuII	CAGCTG	2 cut(s)	836, 1123
RseI	CAYNNNNRTG	1 cut(s)	30
SacI	GAGCTC	1 cut(s)	474
SacII	CCGCGG	1 cut(s)	259
SaqAI	TTAA	2 cut(s)	398, 806
SatI	GCNGC	8 cut(s)	256, 259, 484, 537, 780, 837, 1121, 1134
Sau3AI	GATC	4 cut(s)	496, 787, 1091, 1252
Sau96I	GGNCC	4 cut(s)	191, 253, 442, 814
SchI	GAGTC	1 cut(s)	713
SduI	GDGCHC	3 cut(s)	173, 396, 474
SfaNI	GCATC	7 cut(s)	585, 637, 766, 862, 890, 1120, 1201
Sfr303I	CCGCGG	1 cut(s)	259
SgrAI	CRCCGGYG	1 cut(s)	479
SgrBI	CCGCGG	1 cut(s)	259
SinI	GGWCC	3 cut(s)	191, 442, 814
SmiMI	CAYNNNNRTG	1 cut(s)	30
SmlI	CTYRAG	1 cut(s)	848
SmoI	CTYRAG	1 cut(s)	848
Sse9I	AATT	7 cut(s)	195, 223, 422, 619, 723, 1172, 1375
SsiI	CCGC	8 cut(s)	256, 258, 370, 445, 483, 536, 779, 1260
SstI	GAGCTC	1 cut(s)	474
StyI	CCWWGG	2 cut(s)	510, 758
TaaI	ACNGT	2 cut(s)	927, 1057
TaiI	ACGT	1 cut(s)	222
TaqI	TCGA	2 cut(s)	690, 1195
TaqII	GACCGA	1 cut(s)	716
TasI	AATT	7 cut(s)	195, 223, 422, 619, 723, 1172, 1375
TauI	GCSGC	5 cut(s)	258, 261, 486, 539, 782
TfiI	GAWTC	5 cut(s)	59, 385, 451, 997, 1371
Tru1I	TTAA	2 cut(s)	398, 806
Tru9I	TTAA	2 cut(s)	398, 806
TscAI	CASTG	2 cut(s)	304, 1174
TseFI	GTSAC	3 cut(s)	271, 343, 578
TseI	GCWGC	3 cut(s)	836, 1120, 1133
Tsp45I	GTSAC	3 cut(s)	271, 343, 578
TspDTI	ATGAA	8 cut(s)	39, 676, 878, 935, 953, 989, 1165, 1347
TspGWI	ACGGA	2 cut(s)	217, 302
TspRI	CASTG	2 cut(s)	304, 1174
VpaK11BI	GGWCC	3 cut(s)	191, 442, 814
XagI	CCTNNNNNAGG	1 cut(s)	242
XapI	RAATTY	4 cut(s)	223, 422, 619, 1172
XceI	RCATGY	1 cut(s)	575
XmnI	GAANNNNTTC	2 cut(s)	926, 996
Zsp2I	ATGCAT	2 cut(s)	600, 1194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.