Rh7BG113200

ATP synthase regulation protein NCA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011465
Physical Location & Seq
Reverse (-)
8494150 .. 8497358
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG113200.1

Sequence Viewer

Length: 1791 bp
ATGGAGGTCAGTCCAGCTTCACCGACGGAGGTAAACGAAACCAAAGACCTCAAAACTCTAATCTCCTTCTACTCCCACTACCTCTGGAACCGACTCCTCGATTTCCTCCCCTCTTCCGATTACAATTTTCTCCGCCGAATCCGAGTCCGAGCCGCCGCATCACGGCGGCGACACCTTGCATGTCTTCCGCTCCCTTTACCGGCTAATATACTCGACTCTTGCCAGCCGGCGACGACGGAGGCGTCTAGAGTTCTCGATGTGCTACATGAGATTCTGAAGCACACCTTCTCCAATTTGCACCATGTCCAGAAGAACCTGCAGTTTTGGGAGTCTAGGGCTGAGGGATCAAAAGCTCAAAAAGTTAAGTTTATGGTGCTTGAGAGAGGTCCGCAGGCTTTTGTTGATGGTACAATCCAACTGATACGTGGATATGATGGTGAGGGTTCTTCCATACAGAATATGTGCCTCTCTGCATCGGCTTACATGTCTGAGAGGATAGCGGTATTAAGTAGCTTAAGATCTTCTCTTGCCACATTTTTGGCCCAGTTTTACATGGAAATTGAAAGTTTCGGAGAAGAGTTGGTGAAAGATCCAGAGAGTTCGTTGCCCTCCTTATTAGAGAGAATTAATAACTTGTTCTTCAAATTAGAGGCATCTATCGGCCATCTCCATGCAATGCGCCCGAGTGATTCTTCTGTTGAAGGAAGCTATTCATTTCCGCTAGTATTTGAGAAATTGCCTGAGATAAATCAAGACAGATCCCAGTGGACAGACTGTGAAATTAGGGATTCAATCAATTTGATTTCTCAAAATCTGGAGAAACTGGAATCTCACTTAACTTTTATAGTTGACAAACATCGGAAACCAAGGAGAGTAACTCGACATTGGATCCGCTATACCTGTGGTGCGGTTGGCCTCTCAGTTTGTTCTGTGTGGCTCATAAGACATAGTCGTCTGTCAGGAAGTCATGACATAGATAATTGGGTTCGAGAAGCTAAGGATTCAACCGTCAGCTTCTTGCATGATCATGTTGAGCAACCACTGCTGTCTATTAGAGATGAACTTTTTGCGACATTCAGGCAAAGGCACAAAGGAATGATGGAGCTTGAAGAAGTGCAATTGACCTCCAATTCTCTGCACAGAATGTTGTTGGCTTTCAGTGAGCAGACAAAAGGGCAAAAGTTCCCAGAGAAGGCATCTGATCAGGAAATGCTTGAAATAGTTATGGCTAGGTATGAAAAGGAACTTGAACATCCTATTCATAACCTTGTAAATGGCGAGCTGGCTCGTGCCTTGCTGATCCAGGTTCAGAAGCTAAAACTCGATACTGAAACCGCCATGCTTGAATTGAACCAGATTCTGAGGGCAAATGAGATAAACTTTGCAATCCTAGCTGCTTTACCAGCATTCTTTCTCTCCCTCATTCTGCTAATGCTAGTGCGTGCATGGTTCAAACGGGATAAAAGAGCAGAAGGAAGGGGACGAGTTGCTCGGCTCCAGAGGAGGTTACTTGTTGTGGAAGTTGAGAAGAGGATTATGCAGTATCAAAGTTTTATCGATCAAGGGATGGAGAAAGAAGCGCTGGACATGTTTGGATTGTTGTTGTATAGTCTGGATCGCCTATACCATGCTGTCAAGGGGCATGCAAAAGCAACTGGGGAATGGCAGTGGTTGTGTCAGGATATAGTTGAGCTGGCAAAGCCCAGCCTTCAAACTACATATAAACTCAGAGTGACCTCGCGGATGGAACGGGTTTATGACTGTTTACTTCCTGCATTGCAGCGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NCA2

Protein Analysis

596

Amino Acids

68.84

Weight (kDa)

8.05

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NCA2 PF08637 314 - 586 2.9e-74 ATP synthase regulation protein NCA2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12290 AT5G12290 AT5G12290 AT5G12290
fragaria_vesca FvH4_5g07960
malus_domestica MD03G1163400.v1.1
prunus_persica Prupe.4G269200_v2.0.a1
pyrus_communis pycom5153684g00200
rosa_chinensis RchiOBHm_Chr7g0190441
rosa_laevigata RLG00000004532
rosa_multiflora Rmu_sc0002614.1_g000003 Rmu_sc0029194.1_g000001
rosa_roxburghii Rroxscaffold_3G00264620
rosa_rugosa Rorug06G0504700
rosa_samantha Rh7AG110600 Rh7BG113200 Rh7CG115400 Rh7DG113700
rosa_wichuraiana Rw0G019860 Rw7G009600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 241, 951
Acc36I ACCTGC 1 cut(s) 324
AccBSI CCGCTC 1 cut(s) 190
AccII CGCG 1 cut(s) 1741
AclWI GGATC 7 cut(s) 352, 584, 753, 883, 896, 1294, 1623
AcoI YGGCCR 1 cut(s) 661
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 1 cut(s) 409
AfeI AGCGCT 1 cut(s) 1581
AfiI CCNNNNNNNGG 3 cut(s) 162, 199, 1192
AflII CTTAAG 1 cut(s) 514
AflIII ACRYGT 2 cut(s) 483, 1587
AjnI CCWGG 1 cut(s) 1302
AlwI GGATC 7 cut(s) 352, 584, 753, 883, 896, 1294, 1623
AlwNI CAGNNNCTG 1 cut(s) 1360
Ama87I CYCGRG 1 cut(s) 682
Aor51HI AGCGCT 1 cut(s) 1581
AoxI GGCC 3 cut(s) 540, 661, 913
ApeKI GCWGC 2 cut(s) 1394, 1780
AseI ATTAAT 1 cut(s) 627
Asp700I GAANNNNTTC 1 cut(s) 709
AspLEI GCGC 3 cut(s) 681, 1582, 1785
AspS9I GGNCC 2 cut(s) 386, 541
AsuHPI GGTGA 3 cut(s) 12, 449, 595
AvaI CYCGRG 1 cut(s) 682
AvaII GGWCC 1 cut(s) 386
BamHI GGATCC 1 cut(s) 888
BauI CACGAG 1 cut(s) 1287
BbsI GAAGAC 1 cut(s) 176
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 1 cut(s) 1381
BccI CCATC 6 cut(s) 398, 428, 672, 1093, 1561, 1738
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 1304
BclI TGATCA 2 cut(s) 1024, 1201
BfaI CTAG 6 cut(s) 246, 333, 722, 1230, 1391, 1436
BfmI CTRYAG 1 cut(s) 317
BfoI RGCGCY 2 cut(s) 1583, 1786
BfrI CTTAAG 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 324
BglII AGATCT 1 cut(s) 518
BisI GCNGC 5 cut(s) 153, 156, 167, 1395, 1781
BlsI GCNGC 5 cut(s) 154, 157, 168, 1396, 1782
Bme1390I CCNGG 1 cut(s) 1304
Bme18I GGWCC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 682
BmgT120I GGNCC 2 cut(s) 386, 541
BmiI GGNNCC 3 cut(s) 89, 890, 1496
BmrFI CCNGG 1 cut(s) 1304
BmrI ACTGGG 3 cut(s) 538, 757, 1665
BmsI GCATC 4 cut(s) 167, 482, 662, 1205
BmuI ACTGGG 3 cut(s) 538, 757, 1665
BpiI GAAGAC 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 862, 894
BpmI CTGGAG 2 cut(s) 836, 1481
Bpu10I CCTNAGC 2 cut(s) 339, 996
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 1557
BsaAI YACGTR 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 866
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 199, 1192
Bse118I RCCGGY 2 cut(s) 199, 226
Bse1I ACTGG 5 cut(s) 544, 763, 828, 1660, 1786
Bse3DI GCAATG 2 cut(s) 681, 1775
BseBI CCWGG 1 cut(s) 1304
BseCI ATCGAT 1 cut(s) 1557
BseDI CCNNGG 1 cut(s) 866
BseGI GGATG 3 cut(s) 1252, 1572, 1749
BseLI CCNNNNNNNGG 3 cut(s) 162, 199, 1192
BseMI GCAATG 2 cut(s) 681, 1775
BseMII CTCAG 6 cut(s) 330, 480, 732, 933, 1352, 1741
BseNI ACTGG 5 cut(s) 544, 763, 828, 1660, 1786
BseRI GAGGAG 2 cut(s) 86, 1516
BseXI GCAGC 1 cut(s) 1381
BseYI CCCAGC 1 cut(s) 1703
BsgI GTGCAG 1 cut(s) 1121
Bsh1236I CGCG 1 cut(s) 1741
BshFI GGCC 3 cut(s) 542, 663, 915
BshVI ATCGAT 1 cut(s) 1557
BsiHKCI CYCGRG 1 cut(s) 682
BsiSI CCGG 2 cut(s) 200, 227
BslFI GGGAC 1 cut(s) 1494
BslI CCNNNNNNNGG 3 cut(s) 162, 199, 1192
BsmFI GGGAC 1 cut(s) 1494
BsmI GAATGC 1 cut(s) 1406
BsnI GGCC 3 cut(s) 542, 663, 915
BsoBI CYCGRG 1 cut(s) 682
BspANI GGCC 3 cut(s) 542, 663, 915
BspCNI CTCAG 6 cut(s) 331, 481, 733, 932, 1353, 1740
BspDI ATCGAT 1 cut(s) 1557
BspFNI CGCG 1 cut(s) 1741
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 3 cut(s) 89, 890, 1496
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 7 cut(s) 352, 584, 753, 883, 896, 1294, 1623
BspTI CTTAAG 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 681, 1775
BsrFI RCCGGY 2 cut(s) 199, 226
BsrI ACTGG 5 cut(s) 544, 763, 828, 1660, 1786
BssAI RCCGGY 2 cut(s) 199, 226
BssECI CCNNGG 1 cut(s) 866
BssNI GRCGYC 1 cut(s) 242
BssSI CACGAG 1 cut(s) 1287
BssT1I CCWWGG 1 cut(s) 866
Bst2BI CACGAG 1 cut(s) 1287
Bst2UI CCWGG 1 cut(s) 1304
Bst4CI ACNGT 3 cut(s) 776, 1009, 1763
Bst6I CTCTTC 3 cut(s) 118, 570, 1523
BstACI GRCGYC 1 cut(s) 242
BstAFI CTTAAG 1 cut(s) 514
BstAPI GCANNNNNTGC 1 cut(s) 1042
BstBAI YACGTR 1 cut(s) 425
BstC8I GCNNGC 8 cut(s) 224, 228, 393, 1280, 1284, 1443, 1644, 1695
BstDEI CTNAG 7 cut(s) 339, 489, 741, 919, 996, 1361, 1727
BstF5I GGATG 3 cut(s) 1252, 1572, 1749
BstFNI CGCG 1 cut(s) 1741
BstH2I RGCGCY 2 cut(s) 1583, 1786
BstHHI GCGC 3 cut(s) 681, 1582, 1785
BstMWI GCNNNNNNNGC 4 cut(s) 1042, 1391, 1403, 1699
BstNI CCWGG 1 cut(s) 1304
BstNSI RCATGY 4 cut(s) 183, 487, 1591, 1646
BstSCI CCNGG 1 cut(s) 1302
BstSFI CTRYAG 1 cut(s) 317
BstUI CGCG 1 cut(s) 1741
BstV1I GCAGC 1 cut(s) 1381
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 4 cut(s) 518, 589, 758, 888
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 4 cut(s) 518, 589, 758, 888
Bsu15I ATCGAT 1 cut(s) 1557
BsuRI GGCC 3 cut(s) 542, 663, 915
BsuTUI ATCGAT 1 cut(s) 1557
BtsCI GGATG 3 cut(s) 1252, 1572, 1749
BtsI GCAGTG 2 cut(s) 1040, 1673
BtsIMutI CAGTG 4 cut(s) 770, 1040, 1165, 1673
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 8 cut(s) 224, 228, 393, 1280, 1284, 1443, 1644, 1695
CaiI CAGNNNCTG 1 cut(s) 1360
CciI TCATGA 1 cut(s) 967
CfoI GCGC 3 cut(s) 681, 1582, 1785
Cfr10I RCCGGY 2 cut(s) 199, 226
Cfr13I GGNCC 2 cut(s) 386, 541
ClaI ATCGAT 1 cut(s) 1557
CseI GACGC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 408
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 7 cut(s) 339, 489, 741, 919, 996, 1361, 1727
DrdI GACNNNNNNGTC 2 cut(s) 241, 951
DseDI GACNNNNNNGTC 2 cut(s) 241, 951
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 3 cut(s) 118, 570, 1523
EarI CTCTTC 3 cut(s) 118, 570, 1523
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 866
Eco47I GGWCC 1 cut(s) 386
Eco47III AGCGCT 1 cut(s) 1581
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 1302
EcoT14I CCWWGG 1 cut(s) 866
ErhI CCWWGG 1 cut(s) 866
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 1494
FbaI TGATCA 2 cut(s) 1024, 1201
Fnu4HI GCNGC 5 cut(s) 153, 156, 167, 1395, 1781
FokI GGATG 3 cut(s) 1239, 1579, 1756
Fsp4HI GCNGC 5 cut(s) 153, 156, 167, 1395, 1781
FspBI CTAG 6 cut(s) 246, 333, 722, 1230, 1391, 1436
GlaI GCGC 3 cut(s) 680, 1581, 1784
GluI GCNGC 5 cut(s) 153, 156, 167, 1395, 1781
GsaI CCCAGC 1 cut(s) 1707
GsuI CTGGAG 2 cut(s) 836, 1481
HaeII RGCGCY 2 cut(s) 1583, 1786
HaeIII GGCC 3 cut(s) 542, 663, 915
HapII CCGG 2 cut(s) 200, 227
HgaI GACGC 1 cut(s) 231
HhaI GCGC 3 cut(s) 681, 1582, 1785
Hin1I GRCGYC 1 cut(s) 242
Hin6I GCGC 3 cut(s) 679, 1580, 1783
HinP1I GCGC 3 cut(s) 679, 1580, 1783
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HpaII CCGG 2 cut(s) 200, 227
HphI GGTGA 3 cut(s) 12, 449, 595
Hpy166II GTNNAC 4 cut(s) 34, 768, 850, 1766
Hpy8I GTNNAC 4 cut(s) 34, 768, 850, 1766
Hpy99I CGWCG 3 cut(s) 28, 235, 238
HpyAV CCTTC 7 cut(s) 76, 295, 695, 1186, 1466, 1470, 1718
HpyCH4III ACNGT 3 cut(s) 776, 1009, 1763
HpyCH4IV ACGT 1 cut(s) 424
HpyF10VI GCNNNNNNNGC 4 cut(s) 1042, 1391, 1403, 1699
HpyF3I CTNAG 7 cut(s) 339, 489, 741, 919, 996, 1361, 1727
HpySE526I ACGT 1 cut(s) 424
Hsp92I GRCGYC 1 cut(s) 242
HspAI GCGC 3 cut(s) 679, 1580, 1783
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Ksp22I TGATCA 2 cut(s) 1024, 1201
LmnI GCTCC 3 cut(s) 195, 1102, 1500
Lsp1109I GCAGC 1 cut(s) 1381
LweI GCATC 4 cut(s) 167, 482, 662, 1205
MaeI CTAG 6 cut(s) 246, 333, 722, 1230, 1391, 1436
MaeII ACGT 1 cut(s) 424
MaeIII GTNAC 3 cut(s) 874, 1506, 1732
MbiI CCGCTC 1 cut(s) 190
MfeI CAATTG 1 cut(s) 1118
MflI RGATCY 4 cut(s) 518, 589, 758, 888
MlyI GAGTC 4 cut(s) 87, 153, 209, 338
MmeI TCCRAC 1 cut(s) 439
MroNI GCCGGC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 709
MseI TTAA 5 cut(s) 363, 506, 515, 627, 836
MslI CAYNNNNRTG 2 cut(s) 669, 1026
MspCI CTTAAG 1 cut(s) 514
MspI CCGG 2 cut(s) 200, 227
MspR9I CCNGG 1 cut(s) 1304
MunI CAATTG 1 cut(s) 1118
Mva1269I GAATGC 1 cut(s) 1406
MvaI CCWGG 1 cut(s) 1304
MvnI CGCG 1 cut(s) 1741
MwoI GCNNNNNNNGC 4 cut(s) 1042, 1391, 1403, 1699
NaeI GCCGGC 1 cut(s) 228
NgoMIV GCCGGC 1 cut(s) 226
NlaIV GGNNCC 3 cut(s) 89, 890, 1496
NmeAIII GCCGAG 1 cut(s) 1471
NmuCI GTSAC 1 cut(s) 1732
NspI RCATGY 4 cut(s) 183, 487, 1591, 1646
PaeI GCATGC 1 cut(s) 1646
PagI TCATGA 1 cut(s) 967
PciI ACATGT 2 cut(s) 483, 1587
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 1406
PdiI GCCGGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 709
PfeI GAWTC 7 cut(s) 138, 271, 689, 788, 827, 1001, 1357
PflFI GACNNNGTC 1 cut(s) 948
PkrI GCNGC 5 cut(s) 154, 157, 168, 1396, 1782
PleI GAGTC 4 cut(s) 87, 152, 209, 337
PpsI GAGTC 4 cut(s) 87, 152, 209, 337
Ppu21I YACGTR 1 cut(s) 425
PscI ACATGT 2 cut(s) 483, 1587
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 1302
PspFI CCCAGC 1 cut(s) 1703
PspGI CCWGG 1 cut(s) 1302
PspN4I GGNNCC 3 cut(s) 89, 890, 1496
PspPI GGNCC 2 cut(s) 386, 541
PstI CTGCAG 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 1360
PsuI RGATCY 4 cut(s) 518, 589, 758, 888
PsyI GACNNNGTC 1 cut(s) 948
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
RseI CAYNNNNRTG 2 cut(s) 669, 1026
SaqAI TTAA 5 cut(s) 363, 506, 515, 627, 836
SatI GCNGC 5 cut(s) 153, 156, 167, 1395, 1781
Sau96I GGNCC 2 cut(s) 386, 541
SchI GAGTC 4 cut(s) 87, 153, 209, 338
ScrFI CCNGG 1 cut(s) 1304
SfaNI GCATC 4 cut(s) 167, 482, 662, 1205
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 669, 1026
SmlI CTYRAG 2 cut(s) 377, 514
SmoI CTYRAG 2 cut(s) 377, 514
SphI GCATGC 1 cut(s) 1646
SspMI CTAG 6 cut(s) 246, 333, 722, 1230, 1391, 1436
StyD4I CCNGG 1 cut(s) 1302
StyI CCWWGG 1 cut(s) 866
TaaI ACNGT 3 cut(s) 776, 1009, 1763
TaiI ACGT 1 cut(s) 427
TaqI TCGA 7 cut(s) 99, 213, 255, 880, 988, 1323, 1557
TauI GCSGC 3 cut(s) 155, 158, 169
TfiI GAWTC 7 cut(s) 138, 271, 689, 788, 827, 1001, 1357
Tru1I TTAA 5 cut(s) 363, 506, 515, 627, 836
Tru9I TTAA 5 cut(s) 363, 506, 515, 627, 836
TscAI CASTG 4 cut(s) 770, 1047, 1165, 1673
TseFI GTSAC 1 cut(s) 1732
TseI GCWGC 2 cut(s) 1394, 1780
Tsp45I GTSAC 1 cut(s) 1732
TspDTI ATGAA 4 cut(s) 702, 1074, 1250, 1251
TspGWI ACGGA 2 cut(s) 41, 251
TspRI CASTG 4 cut(s) 770, 1047, 1165, 1673
Tth111I GACNNNGTC 1 cut(s) 948
Vha464I CTTAAG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 386
VspI ATTAAT 1 cut(s) 627
XbaI TCTAGA 1 cut(s) 245
XceI RCATGY 4 cut(s) 183, 487, 1591, 1646
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 709
XspI CTAG 6 cut(s) 246, 333, 722, 1230, 1391, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.