Rw0G019860

ATP synthase regulation protein NCA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00893
OG0011465
Physical Location & Seq
Reverse (-)
24982 .. 27305
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019860.1

Sequence Viewer

Length: 1761 bp
ATGGAGGTCAGTCCAGCTTCACCGACGGAGGTAAACGAAACCAAAGACCTCAAAACCCTAATCTCCTTCTACTCCCACTACCTCTGGAACCGACTCCTCGATCTCCTCCCCTCTTCCGATTACAATTTTCTCCGCAGAATCCGAGTCCGAGCCGCCGCATCACGGCGGAGACACCGCGCATGTCTTCCGCTCCCCTTACCGGCTAATATACTCGACTCTTTTCTCGATGTTCTACATGAGATTCTGAAGCACACCTTCTCCAATTTGCACCATGTCCAGAAGAACCTGCAGTTTTGGGAGTCTAGGGCTGAGGGATCAAAAGCTCAAAAAGTTAAGTTTATGGTGCTTCAGAGAGGTCCGCAGGCTTTTGTTGATGGTACAATCCAACTGATATGTGGATATGATGGTGAGGGTTCTTCCATACAGAATATGTGCCGCTCTGCATCGGCTTACATGTCTGAGAGGATAGCAGTATTAAGTAGCTTAAGATCTTCTCTTGCCACATTTTTGGCCCAGTTTTACATGGAAATTGAAAGATTCGGAGAAGAGTTGGTGAAAGATCCAGAGAGTTCATTGCCCTCCTTATTAGAGAGAATTAATGACTTGTTCTTCAAATTAGAGGCGTCTATCGGCCATCTCCATGCAATGCGCCCGAGTGATTCTTCTGTTGAAGGAAGCTATTCATTTCCCCTAGTATTTGAGAAATTGCCCGAGATAAATCAAGACAGATCCCAGTGGACAGACTGTGAAATTAGGGATTCAATCAATTTGATTTCTCAAAATTTGGAGAAACTGGAATCTCACTTAACTTTTATAGTTGACAAACATCGGAAACCAAGGAGAGTAACTCGATATTGGATCCCCTATACTTGTGGTGCGGTTGGCCTCTCAGTTTGTTCTGTGTGGCTCATAAGACATAGTCGTCTGTCAGGAAGTCATGACATAGATAATTGGGTTCGAGAAGCTAAGGATTCAACCGTCAGCTTCTTGCGTGATCATGTTGAGCAACCGCTGCTGTCTATTAGAGATGAACTTTTTAAGACATTCAGGCAAAGGCACAAAGGAATGATGGAGCTTGAAGAAGTGCAATTAACCTCCAATTCTCTGCACAGAATGTTGTTGGCTTTCAGTGAGCAGACAAAAGGGCAAAATTTCCCAGAGAATGCATCTGATCAGGAAATGCTTGAAATAGTTATGGCTAGGTATGAAAAGGAACTTGAACATCCTATTCATAACCTTGTAAATGGCGAGCTGGCTCGTGCCTTGCTGATCCAGGTTCAGAAGCTAAAACTCGATACTGAAACCGCCATGCTTGAATTGAACCAGATTCTGAGGGCAAATGAGATAAACTTTGCAATCCTAGCTGCTTTACCAGCATTCTTTCTCTCCCTCATTCTGCTAATGCTAGTGCGTGCATGGTTCAAACGGGATAAAAGAGCAGAAGGAAGGGGACGAGTTGCTCGGCTCCAGAGGAGGTTACTTGTTGTGGAAGTTGAGAAGAGGATTATGCAGTATCAAAGTTTTATCGATCAAGGGATGGAGAAAGAAGCGCTAGACATGTTCGGATTGTTGTTGTATAGTCTGGATCGCCTATACCATGCTGTCAAGGGGCATGCAAAAGCAACTGGGGAATGGCAGTGGTTGTGTCAGGATATAGTTGACCTGGCAAAGCCCAGCCTTCAAACTGCATTTAAACTCAGTGTGACCTCGCGGTTGGAACGGGTTTATGACTGTTTACTTCCTGCATTGCAGCGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NCA2

Protein Analysis

586

Amino Acids

67.77

Weight (kDa)

8.04

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NCA2 PF08637 304 - 576 3.8e-73 ATP synthase regulation protein NCA2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12290 AT5G12290 AT5G12290 AT5G12290
fragaria_vesca FvH4_5g07960
malus_domestica MD03G1163400.v1.1
prunus_persica Prupe.4G269200_v2.0.a1
pyrus_communis pycom5153684g00200
rosa_chinensis RchiOBHm_Chr7g0190441
rosa_laevigata RLG00000004532
rosa_multiflora Rmu_sc0002614.1_g000003 Rmu_sc0029194.1_g000001
rosa_roxburghii Rroxscaffold_3G00264620
rosa_rugosa Rorug06G0504700
rosa_samantha Rh7AG110600 Rh7BG113200 Rh7CG115400 Rh7DG113700
rosa_wichuraiana Rw0G019860 Rw7G009600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 921
Acc36I ACCTGC 1 cut(s) 294
AccBSI CCGCTC 2 cut(s) 190, 438
AccII CGCG 2 cut(s) 177, 1711
AclWI GGATC 7 cut(s) 322, 554, 723, 853, 866, 1264, 1593
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 2 cut(s) 781, 1150
AcuI CTGAAG 2 cut(s) 266, 332
AcyI GRCGYC 1 cut(s) 623
AfaI GTAC 1 cut(s) 379
AfeI AGCGCT 1 cut(s) 1551
AfiI CCNNNNNNNGG 2 cut(s) 162, 199
AflII CTTAAG 1 cut(s) 484
AflIII ACRYGT 2 cut(s) 453, 1557
AjnI CCWGG 2 cut(s) 1272, 1662
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 7 cut(s) 322, 554, 723, 853, 866, 1264, 1593
AlwNI CAGNNNCTG 1 cut(s) 1330
Ama87I CYCGRG 2 cut(s) 652, 710
Aor51HI AGCGCT 1 cut(s) 1551
AoxI GGCC 3 cut(s) 510, 631, 883
ApeKI GCWGC 3 cut(s) 1012, 1364, 1750
ApoI RAATTY 2 cut(s) 781, 1150
AseI ATTAAT 1 cut(s) 597
Asp700I GAANNNNTTC 1 cut(s) 679
AspLEI GCGC 4 cut(s) 179, 651, 1552, 1755
AspS9I GGNCC 2 cut(s) 356, 511
AsuHPI GGTGA 3 cut(s) 12, 419, 565
AvaI CYCGRG 2 cut(s) 652, 710
AvaII GGWCC 1 cut(s) 356
BamHI GGATCC 1 cut(s) 858
BauI CACGAG 1 cut(s) 1257
BbsI GAAGAC 1 cut(s) 176
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 999, 1351
BccI CCATC 5 cut(s) 368, 398, 642, 1063, 1531
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 1274, 1664
BclI TGATCA 2 cut(s) 994, 1171
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 6 cut(s) 303, 692, 1200, 1361, 1406, 1553
BfmI CTRYAG 1 cut(s) 287
BfoI RGCGCY 2 cut(s) 1553, 1756
BfrI CTTAAG 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 294
BglII AGATCT 1 cut(s) 488
BisI GCNGC 6 cut(s) 153, 156, 436, 1013, 1365, 1751
BlsI GCNGC 6 cut(s) 154, 157, 437, 1014, 1366, 1752
Bme1390I CCNGG 2 cut(s) 1274, 1664
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 2 cut(s) 652, 710
BmgT120I GGNCC 2 cut(s) 356, 511
BmiI GGNNCC 3 cut(s) 89, 860, 1466
BmrFI CCNGG 2 cut(s) 1274, 1664
BmrI ACTGGG 3 cut(s) 508, 727, 1635
BmsI GCATC 3 cut(s) 167, 452, 1175
BmuI ACTGGG 3 cut(s) 508, 727, 1635
BpiI GAAGAC 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 832, 864
BpmI CTGGAG 1 cut(s) 1451
Bpu10I CCTNAGC 2 cut(s) 309, 966
Bsa29I ATCGAT 1 cut(s) 1527
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 836
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 199
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 5 cut(s) 514, 733, 798, 1630, 1756
Bse3DI GCAATG 3 cut(s) 572, 651, 1745
BseBI CCWGG 2 cut(s) 1274, 1664
BseCI ATCGAT 1 cut(s) 1527
BseDI CCNNGG 1 cut(s) 836
BseGI GGATG 2 cut(s) 1222, 1542
BseLI CCNNNNNNNGG 2 cut(s) 162, 199
BseMI GCAATG 3 cut(s) 572, 651, 1745
BseMII CTCAG 5 cut(s) 300, 450, 903, 1322, 1711
BseNI ACTGG 5 cut(s) 514, 733, 798, 1630, 1756
BseRI GAGGAG 3 cut(s) 86, 95, 1486
BseXI GCAGC 2 cut(s) 999, 1351
BseYI CCCAGC 1 cut(s) 1673
BsgI GTGCAG 1 cut(s) 1091
Bsh1236I CGCG 2 cut(s) 177, 1711
BshFI GGCC 3 cut(s) 512, 633, 885
BshVI ATCGAT 1 cut(s) 1527
BsiHKCI CYCGRG 2 cut(s) 652, 710
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 1464
BslI CCNNNNNNNGG 2 cut(s) 162, 199
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 1464
BsmI GAATGC 2 cut(s) 1168, 1376
BsnI GGCC 3 cut(s) 512, 633, 885
BsoBI CYCGRG 2 cut(s) 652, 710
BspANI GGCC 3 cut(s) 512, 633, 885
BspCNI CTCAG 5 cut(s) 301, 451, 902, 1323, 1710
BspDI ATCGAT 1 cut(s) 1527
BspFNI CGCG 2 cut(s) 177, 1711
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 3 cut(s) 89, 860, 1466
BspMAI CTGCAG 1 cut(s) 291
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 7 cut(s) 322, 554, 723, 853, 866, 1264, 1593
BspTI CTTAAG 1 cut(s) 484
BsrBI CCGCTC 2 cut(s) 190, 438
BsrDI GCAATG 3 cut(s) 572, 651, 1745
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 5 cut(s) 514, 733, 798, 1630, 1756
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 836
BssNI GRCGYC 1 cut(s) 623
BssSI CACGAG 1 cut(s) 1257
BssT1I CCWWGG 1 cut(s) 836
Bst2BI CACGAG 1 cut(s) 1257
Bst2UI CCWGG 2 cut(s) 1274, 1664
Bst4CI ACNGT 3 cut(s) 746, 979, 1733
Bst6I CTCTTC 3 cut(s) 118, 540, 1493
BstACI GRCGYC 1 cut(s) 623
BstAFI CTTAAG 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstC8I GCNNGC 5 cut(s) 363, 1250, 1254, 1413, 1614
BstDEI CTNAG 6 cut(s) 309, 459, 889, 966, 1331, 1697
BstF5I GGATG 2 cut(s) 1222, 1542
BstFNI CGCG 2 cut(s) 177, 1711
BstH2I RGCGCY 2 cut(s) 1553, 1756
BstHHI GCGC 4 cut(s) 179, 651, 1552, 1755
BstMAI GTCTC 1 cut(s) 163
BstMWI GCNNNNNNNGC 3 cut(s) 1012, 1361, 1373
BstNI CCWGG 2 cut(s) 1274, 1664
BstNSI RCATGY 4 cut(s) 183, 457, 1561, 1616
BstSCI CCNGG 2 cut(s) 1272, 1662
BstSFI CTRYAG 1 cut(s) 287
BstUI CGCG 2 cut(s) 177, 1711
BstV1I GCAGC 2 cut(s) 999, 1351
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 4 cut(s) 488, 559, 728, 858
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 4 cut(s) 488, 559, 728, 858
Bsu15I ATCGAT 1 cut(s) 1527
BsuRI GGCC 3 cut(s) 512, 633, 885
BsuTUI ATCGAT 1 cut(s) 1527
BtsCI GGATG 2 cut(s) 1222, 1542
BtsI GCAGTG 1 cut(s) 1643
BtsIMutI CAGTG 4 cut(s) 740, 1135, 1643, 1705
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 5 cut(s) 363, 1250, 1254, 1413, 1614
CaiI CAGNNNCTG 1 cut(s) 1330
CciI TCATGA 1 cut(s) 937
CfoI GCGC 4 cut(s) 179, 651, 1552, 1755
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 356, 511
ClaI ATCGAT 1 cut(s) 1527
CseI GACGC 1 cut(s) 612
Csp6I GTAC 1 cut(s) 378
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 6 cut(s) 309, 459, 889, 966, 1331, 1697
DraI TTTAAA 1 cut(s) 1693
DrdI GACNNNNNNGTC 1 cut(s) 921
DseDI GACNNNNNNGTC 1 cut(s) 921
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 3 cut(s) 118, 540, 1493
EarI CTCTTC 3 cut(s) 118, 540, 1493
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 1551
Eco57I CTGAAG 2 cut(s) 266, 332
Eco88I CYCGRG 2 cut(s) 652, 710
EcoRII CCWGG 2 cut(s) 1272, 1662
EcoT14I CCWWGG 1 cut(s) 836
EcoT22I ATGCAT 1 cut(s) 1168
ErhI CCWWGG 1 cut(s) 836
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 1464
FbaI TGATCA 2 cut(s) 994, 1171
Fnu4HI GCNGC 6 cut(s) 153, 156, 436, 1013, 1365, 1751
FokI GGATG 2 cut(s) 1209, 1549
Fsp4HI GCNGC 6 cut(s) 153, 156, 436, 1013, 1365, 1751
FspBI CTAG 6 cut(s) 303, 692, 1200, 1361, 1406, 1553
GlaI GCGC 4 cut(s) 178, 650, 1551, 1754
GluI GCNGC 6 cut(s) 153, 156, 436, 1013, 1365, 1751
GsaI CCCAGC 1 cut(s) 1677
GsuI CTGGAG 1 cut(s) 1451
HaeII RGCGCY 2 cut(s) 1553, 1756
HaeIII GGCC 3 cut(s) 512, 633, 885
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 612
HhaI GCGC 4 cut(s) 179, 651, 1552, 1755
Hin1I GRCGYC 1 cut(s) 623
Hin6I GCGC 4 cut(s) 177, 649, 1550, 1753
HinP1I GCGC 4 cut(s) 177, 649, 1550, 1753
HincII GTYRAC 2 cut(s) 820, 1660
HindII GTYRAC 2 cut(s) 820, 1660
HpaII CCGG 1 cut(s) 200
HphI GGTGA 3 cut(s) 12, 419, 565
Hpy166II GTNNAC 5 cut(s) 34, 738, 820, 1660, 1736
Hpy8I GTNNAC 5 cut(s) 34, 738, 820, 1660, 1736
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 6 cut(s) 76, 265, 665, 1436, 1440, 1688
HpyCH4III ACNGT 3 cut(s) 746, 979, 1733
HpyF10VI GCNNNNNNNGC 3 cut(s) 1012, 1361, 1373
HpyF3I CTNAG 6 cut(s) 309, 459, 889, 966, 1331, 1697
Hsp92I GRCGYC 1 cut(s) 623
HspAI GCGC 4 cut(s) 177, 649, 1550, 1753
Ksp22I TGATCA 2 cut(s) 994, 1171
LmnI GCTCC 3 cut(s) 195, 1072, 1470
Lsp1109I GCAGC 2 cut(s) 999, 1351
LweI GCATC 3 cut(s) 167, 452, 1175
MaeI CTAG 6 cut(s) 303, 692, 1200, 1361, 1406, 1553
MaeIII GTNAC 3 cut(s) 844, 1476, 1702
MbiI CCGCTC 2 cut(s) 190, 438
MflI RGATCY 4 cut(s) 488, 559, 728, 858
MlyI GAGTC 4 cut(s) 87, 153, 209, 308
MmeI TCCRAC 2 cut(s) 409, 1695
Mph1103I ATGCAT 1 cut(s) 1168
MroXI GAANNNNTTC 1 cut(s) 679
MseI TTAA 8 cut(s) 333, 476, 485, 597, 806, 1038, 1091, 1692
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 1012
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 1274, 1664
Mva1269I GAATGC 2 cut(s) 1168, 1376
MvaI CCWGG 2 cut(s) 1274, 1664
MvnI CGCG 2 cut(s) 177, 1711
MwoI GCNNNNNNNGC 3 cut(s) 1012, 1361, 1373
NlaIV GGNNCC 3 cut(s) 89, 860, 1466
NmeAIII GCCGAG 1 cut(s) 1441
NmuCI GTSAC 1 cut(s) 1702
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 4 cut(s) 183, 457, 1561, 1616
PaeI GCATGC 1 cut(s) 1616
PagI TCATGA 1 cut(s) 937
PciI ACATGT 2 cut(s) 453, 1557
PctI GAATGC 2 cut(s) 1168, 1376
PdmI GAANNNNTTC 1 cut(s) 679
PfeI GAWTC 8 cut(s) 138, 241, 537, 659, 758, 797, 971, 1327
PflFI GACNNNGTC 1 cut(s) 918
PkrI GCNGC 6 cut(s) 154, 157, 437, 1014, 1366, 1752
PleI GAGTC 4 cut(s) 87, 152, 209, 307
PpsI GAGTC 4 cut(s) 87, 152, 209, 307
PscI ACATGT 2 cut(s) 453, 1557
PshBI ATTAAT 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 1272, 1662
PspFI CCCAGC 1 cut(s) 1673
PspGI CCWGG 2 cut(s) 1272, 1662
PspN4I GGNNCC 3 cut(s) 89, 860, 1466
PspPI GGNCC 2 cut(s) 356, 511
PstI CTGCAG 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 1330
PsuI RGATCY 4 cut(s) 488, 559, 728, 858
PsyI GACNNNGTC 1 cut(s) 918
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 639
SaqAI TTAA 8 cut(s) 333, 476, 485, 597, 806, 1038, 1091, 1692
SatI GCNGC 6 cut(s) 153, 156, 436, 1013, 1365, 1751
Sau96I GGNCC 2 cut(s) 356, 511
SchI GAGTC 4 cut(s) 87, 153, 209, 308
ScrFI CCNGG 2 cut(s) 1274, 1664
SfaNI GCATC 3 cut(s) 167, 452, 1175
SfcI CTRYAG 1 cut(s) 287
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
SphI GCATGC 1 cut(s) 1616
SspMI CTAG 6 cut(s) 303, 692, 1200, 1361, 1406, 1553
StyD4I CCNGG 2 cut(s) 1272, 1662
StyI CCWWGG 1 cut(s) 836
TaaI ACNGT 3 cut(s) 746, 979, 1733
TaqI TCGA 7 cut(s) 99, 213, 225, 850, 958, 1293, 1527
TauI GCSGC 3 cut(s) 155, 158, 438
TfiI GAWTC 8 cut(s) 138, 241, 537, 659, 758, 797, 971, 1327
Tru1I TTAA 8 cut(s) 333, 476, 485, 597, 806, 1038, 1091, 1692
Tru9I TTAA 8 cut(s) 333, 476, 485, 597, 806, 1038, 1091, 1692
TscAI CASTG 4 cut(s) 740, 1135, 1643, 1705
TseFI GTSAC 1 cut(s) 1702
TseI GCWGC 3 cut(s) 1012, 1364, 1750
Tsp45I GTSAC 1 cut(s) 1702
TspDTI ATGAA 5 cut(s) 561, 672, 1044, 1220, 1221
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 4 cut(s) 740, 1135, 1643, 1705
Tth111I GACNNNGTC 1 cut(s) 918
Vha464I CTTAAG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 356
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 2 cut(s) 781, 1150
XceI RCATGY 4 cut(s) 183, 457, 1561, 1616
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 679
XspI CTAG 6 cut(s) 303, 692, 1200, 1361, 1406, 1553
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.