Rh7DG212000

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0018995
Physical Location & Seq
Forward (+)
20433961 .. 20434314
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG212000.1

Sequence Viewer

Length: 288 bp
ATGGACTGTCCTCGTCGCTCCTCCGTCATCTTAATCAGTGTTGGAGTCTCCGGTGAGAGCCAAAACCTTTCTTCGATCACTTCAATCAGCTTCGTTAAGTCGTTTGATCAAGAGGCGAAGCTTTTGATCGAGAACGGCATAGAGAACGTAGTGATTCTCGAAGCTTCGGACCGCATCTGCAGTAGGATCCGAAAACAAGAATTCGGAGGAGTGTCGGTGGAGCTCAGCGCGGGTTGGATCGTCGGAGTTAGTGGAGAACAGCTTAATCTGGTTTGGGGAGCTCGCTGA

Protein Analysis

95

Amino Acids

10.27

Weight (kDa)

5.02

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amino_oxidase PF01593 39 - 84 4.5e-06 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018995)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0457801 RchiOBHm_Chr4g0413091
rosa_multiflora Rmu_sc0007827.1_g000001
rosa_roxburghii Rroxscaffold_2G00138640
rosa_samantha Rh2AG469900 Rh2CG456000 Rh4AG177800 Rh7CG218000 Rh7DG212000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 230
AciI CCGC 2 cut(s) 172, 230
AclWI GGATC 3 cut(s) 181, 194, 245
AcsI RAATTY 1 cut(s) 200
AgsI TTSAA 1 cut(s) 84
AluBI AGCT 6 cut(s) 90, 121, 164, 223, 262, 281
AluI AGCT 6 cut(s) 90, 121, 164, 223, 262, 281
Alw21I GWGCWC 2 cut(s) 225, 283
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 3 cut(s) 181, 194, 245
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 169
BamHI GGATCC 1 cut(s) 186
BanII GRGCYC 2 cut(s) 225, 283
Bbv12I GWGCWC 2 cut(s) 225, 283
BceAI ACGGC 1 cut(s) 151
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 52
BfmI CTRYAG 1 cut(s) 178
BlpI GCTNAGC 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 188
BmsI GCATC 1 cut(s) 183
Bpu1102I GCTNAGC 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
BseMII CTCAG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 10, 222
Bsh1236I CGCG 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 225, 283
BsiSI CCGG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 225, 283
Bsp143I GATC 5 cut(s) 75, 106, 126, 186, 237
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 2 cut(s) 172, 230
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 1 cut(s) 182
BspPI GGATC 3 cut(s) 181, 194, 245
BssMI GATC 5 cut(s) 75, 106, 126, 186, 237
Bst4CI ACNGT 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 1 cut(s) 224
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 5 cut(s) 78, 109, 129, 189, 240
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 5 cut(s) 75, 106, 126, 186, 237
BstSFI CTRYAG 1 cut(s) 178
BstUI CGCG 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 169
CpoI CGGWCCG 1 cut(s) 169
CspI CGGWCCG 1 cut(s) 169
CviJI RGCY 7 cut(s) 60, 90, 121, 164, 223, 262, 281
CviKI_1 RGCY 7 cut(s) 60, 90, 121, 164, 223, 262, 281
DdeI CTNAG 1 cut(s) 224
DpnI GATC 5 cut(s) 77, 108, 128, 188, 239
DpnII GATC 5 cut(s) 75, 106, 126, 186, 237
Ecl136II GAGCTC 2 cut(s) 223, 281
Eco24I GRGCYC 2 cut(s) 225, 283
Eco47I GGWCC 1 cut(s) 169
Eco53kI GAGCTC 2 cut(s) 223, 281
EcoICRI GAGCTC 2 cut(s) 223, 281
EcoRI GAATTC 1 cut(s) 200
EcoT38I GRGCYC 2 cut(s) 225, 283
FaiI YATR 1 cut(s) 140
FauI CCCGC 1 cut(s) 223
FbaI TGATCA 1 cut(s) 106
FriOI GRGCYC 2 cut(s) 225, 283
GlaI GCGC 1 cut(s) 229
HapII CCGG 1 cut(s) 51
HhaI GCGC 1 cut(s) 230
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HindIII AAGCTT 2 cut(s) 119, 162
HinfI GANTC 2 cut(s) 45, 154
HpaII CCGG 1 cut(s) 51
HphI GGTGA 1 cut(s) 65
Hpy188I TCNGA 4 cut(s) 169, 191, 206, 245
Hpy188III TCNNGA 3 cut(s) 110, 130, 158
Hpy99I CGWCG 2 cut(s) 18, 245
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 180
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 147
HspAI GCGC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 5 cut(s) 75, 106, 126, 186, 237
LmnI GCTCC 3 cut(s) 23, 220, 278
LpnPI CCDG 2 cut(s) 64, 254
LweI GCATC 1 cut(s) 183
MaeII ACGT 1 cut(s) 147
MalI GATC 5 cut(s) 77, 108, 128, 188, 239
MboI GATC 5 cut(s) 75, 106, 126, 186, 237
MboII GAAGA 1 cut(s) 63
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 2 cut(s) 225, 283
MluCI AATT 1 cut(s) 200
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 3 cut(s) 22, 215, 223
MnlI CCTC 4 cut(s) 21, 31, 106, 200
MseI TTAA 3 cut(s) 32, 96, 264
MspI CCGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 230
NdeII GATC 5 cut(s) 75, 106, 126, 186, 237
NlaIV GGNNCC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 154
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp124BI GAGCTC 2 cut(s) 225, 283
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 169
PstI CTGCAG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 186
Rsr2I CGGWCCG 1 cut(s) 169
RsrII CGGWCCG 1 cut(s) 169
SacI GAGCTC 2 cut(s) 225, 283
SaqAI TTAA 3 cut(s) 32, 96, 264
Sau3AI GATC 5 cut(s) 75, 106, 126, 186, 237
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 1 cut(s) 54
SduI GDGCHC 2 cut(s) 225, 283
SetI ASST 8 cut(s) 69, 92, 123, 150, 166, 225, 264, 283
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 1 cut(s) 178
SgeI CNNG 9 cut(s) 24, 63, 122, 142, 170, 209, 241, 243, 281
SinI GGWCC 1 cut(s) 169
Sse9I AATT 1 cut(s) 200
SsiI CCGC 2 cut(s) 172, 230
SstI GAGCTC 2 cut(s) 225, 283
TaaI ACNGT 1 cut(s) 8
TaiI ACGT 1 cut(s) 150
TaqI TCGA 3 cut(s) 74, 129, 159
TasI AATT 1 cut(s) 200
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 3 cut(s) 32, 96, 264
Tru9I TTAA 3 cut(s) 32, 96, 264
TscAI CASTG 1 cut(s) 43
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.