Rw1G032360

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015459
Physical Location & Seq
Forward (+)
60655390 .. 60656391
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032360.1

Sequence Viewer

Length: 1002 bp
ATGTTCCTCCTTCGTAAAATCCCCCACAAACCCCAAAATTCCAAATCAATCCTCACCGTCGCCGCCCGTTTCTGCACAACCCAAATCCCTACCGTCAATTCTTTTCCCGATGATCCCACCGCCGCCTACTACGACCTCCTGGTCAACGCCGCCGGCGAATCCAGAGACTTCGCCAACCTCCAGTACCTCCTCAACAAGCGCGTGAAGGACCACTGCTTCAACACCTCCAACACCTTCAAGTTCATCACCAACACCGGGGCTGCCCTCTCCGTACTCCCCGATCTCTCTCGGACCCTCGCGCGTCTCGACAAAGGGTTCACGCGCAAGAGCGCGTACGACTCGCTCATCTCCCGCCTCTGCAAGCTGGAGCTAGTCGAGGAGGCGCTGCGCGTGGTGGACCTGATGGCGCGTGAGAATTTCGGCCTGAACGCGTGTACCTTCCACCCGATTCTCAACGTGCTTACAAAGAAGGGGAAGATGGAAGAGGCGTGGCGCGTGGTGGAGCTGATGAGGAAATCCGGAGCTCCGCCGGATCTCACGTCGTATAATTATCTTCTCATGGCGTATTGCTTCTCCGGAGATTTGGATCCGGCGGCCGGAGTTTTGTGTACTATGGAGGAGGAGGGGATGAAGGCGGACACGCGTACGTACGACGCGCTGGTGCTTGGCGCGTGCAAGGTGGGGCAGGTGGAGGGGGCTATGGTGCTGCTGAGGAGGATGGTGGATGATGGAGTTCCGGTGCTGCTATCGACCCACGTGTACGTTATTGATTCGCTCTTGAGTCGGGGGTTTTACGCGGAAGCGGTAAAGTTTGTGAGGGGTTTTAGCGGGAAGGACACTTGGTTGGACAAGGAGAGTTTTGGGAGTCTGGCTAAGAGGCTCATTAAGCTCGAGAGGCTTGGTGAGGCGAAGGAGGTCTTGGATGAAATGCGTGAAAGGGGTTTGGTTATTGGAGATAAATTGAAGGATTATTATGAATCAAAGATTACAAATAAGAAATAA

Protein Analysis

333

Amino Acids

37.34

Weight (kDa)

8.68

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 108 - 200 1.8e-09 Fungal tetratrico peptide repeats
PPR_long PF17177 125 - 243 7.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 130 - 189 2.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 143 - 190 1.4e-09 PPR repeat family
TPR_24 PF23276 164 - 270 7.3e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 177 - 226 1.9e-08 PPR repeat family
PPR_1 PF12854 209 - 241 6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40240 AT3G56030
fragaria_vesca FvH4_7g24790
pyrus_communis pycom01g17280
rosa_chinensis RchiOBHm_Chr1g0370651
rosa_laevigata RLG00000027021
rosa_multiflora Rmu_sc0002021.1_g000005 Rmu_sc0003210.1_g000001 Rmu_sc0004550.1_g000001
rosa_roxburghii Rroxscaffold_4G00286370
rosa_rugosa Rorug01G0358100
rosa_samantha Rh1AG367800 Rh1BG331300 Rh1CG345400 Rh1DG362700
rosa_wichuraiana Rw1G032360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 676
AasI GACNNNNNNGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 676
AccIII TCCGGA 2 cut(s) 518, 575
AclWI GGATC 4 cut(s) 107, 540, 581, 594
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 2 cut(s) 37, 415
AcvI CACGTG 1 cut(s) 757
AfaI GTAC 8 cut(s) 185, 273, 335, 436, 610, 646, 650, 761
AfiI CCNNNNNNNGG 2 cut(s) 255, 596
AflIII ACRYGT 3 cut(s) 429, 641, 756
AgsI TTSAA 3 cut(s) 220, 238, 964
AjiI CACGTC 1 cut(s) 540
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AluBI AGCT 5 cut(s) 364, 370, 505, 524, 889
AluI AGCT 5 cut(s) 364, 370, 505, 524, 889
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 2 cut(s) 159, 308
AlwI GGATC 4 cut(s) 107, 540, 581, 594
Ama87I CYCGRG 1 cut(s) 890
Aor13HI TCCGGA 2 cut(s) 518, 575
AoxI GGCC 2 cut(s) 421, 594
ApeKI GCWGC 4 cut(s) 260, 385, 706, 742
ApoI RAATTY 2 cut(s) 37, 415
AspS9I GGNCC 3 cut(s) 208, 291, 397
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 3 cut(s) 46, 238, 914
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 3 cut(s) 208, 291, 397
BaeI ACNNNNGTAYC 2 cut(s) 418, 451
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 526
BbrPI CACGTG 1 cut(s) 757
Bbv12I GWGCWC 1 cut(s) 526
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 4 cut(s) 247, 372, 693, 729
BccI CCATC 4 cut(s) 397, 472, 712, 722
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 159, 308
BfaI CTAG 1 cut(s) 371
BfoI RGCGCY 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 676
BisI GCNGC 8 cut(s) 63, 123, 150, 261, 386, 594, 707, 743
BlsI GCNGC 8 cut(s) 64, 124, 151, 262, 387, 595, 708, 744
Bme1390I CCNGG 2 cut(s) 140, 256
Bme18I GGWCC 3 cut(s) 208, 291, 397
BmeT110I CYCGRG 1 cut(s) 890
BmgBI CACGTC 1 cut(s) 540
BmgT120I GGNCC 3 cut(s) 208, 291, 397
BmiI GGNNCC 2 cut(s) 293, 588
BmrFI CCNGG 2 cut(s) 140, 256
BpmI CTGGAG 2 cut(s) 164, 386
Bpu10I CCTNAGC 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 256
BsaAI YACGTR 2 cut(s) 648, 757
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 1 cut(s) 255
BsaWI WCCGGW 3 cut(s) 518, 575, 736
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 596
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 2 cut(s) 518, 575
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 4 cut(s) 633, 723, 730, 928
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 255, 596
BseMII CTCAG 1 cut(s) 701
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 5 cut(s) 179, 392, 632, 635, 727
BseX3I CGGCCG 1 cut(s) 594
BseXI GCAGC 4 cut(s) 247, 372, 693, 729
BsgI GTGCAG 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 2 cut(s) 423, 596
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 526
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 8 cut(s) 153, 255, 519, 530, 576, 590, 597, 737
BsiWI CGTACG 3 cut(s) 333, 644, 648
BslI CCNNNNNNNGG 2 cut(s) 255, 596
BsmAI GTCTC 2 cut(s) 159, 308
BsmBI CGTCTC 1 cut(s) 308
BsnI GGCC 2 cut(s) 423, 596
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 526
Bsp13I TCCGGA 2 cut(s) 518, 575
Bsp143I GATC 4 cut(s) 112, 280, 532, 586
BspANI GGCC 2 cut(s) 423, 596
BspCNI CTCAG 1 cut(s) 702
BspEI TCCGGA 2 cut(s) 518, 575
BspLI GGNNCC 2 cut(s) 293, 588
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 4 cut(s) 107, 540, 581, 594
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 4 cut(s) 112, 280, 532, 586
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 58, 94
Bst6I CTCTTC 1 cut(s) 477
BstBAI YACGTR 2 cut(s) 648, 757
BstC8I GCNNGC 3 cut(s) 154, 362, 673
BstDEI CTNAG 2 cut(s) 710, 873
BstF5I GGATG 4 cut(s) 633, 723, 730, 928
BstH2I RGCGCY 1 cut(s) 386
BstKTI GATC 4 cut(s) 115, 283, 535, 589
BstMAI GTCTC 2 cut(s) 159, 308
BstMBI GATC 4 cut(s) 112, 280, 532, 586
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 2 cut(s) 886, 895
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 138, 254
BstSNI TACGTA 1 cut(s) 648
BstV1I GCAGC 4 cut(s) 247, 372, 693, 729
BstX2I RGATCY 2 cut(s) 532, 586
BstYI RGATCY 2 cut(s) 532, 586
BstZI CGGCCG 1 cut(s) 594
BsuRI GGCC 2 cut(s) 423, 596
BtrI CACGTC 1 cut(s) 540
BtsCI GGATG 4 cut(s) 633, 723, 730, 928
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 211
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 3 cut(s) 154, 362, 673
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 208, 291, 397
CseI GACGC 2 cut(s) 290, 662
Csp6I GTAC 8 cut(s) 184, 272, 334, 435, 609, 645, 649, 760
CviAII CATG 1 cut(s) 559
CviQI GTAC 8 cut(s) 184, 272, 334, 435, 609, 645, 649, 760
DdeI CTNAG 2 cut(s) 710, 873
DpnI GATC 4 cut(s) 114, 282, 534, 588
DpnII GATC 4 cut(s) 112, 280, 532, 586
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 594
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
EciI GGCGGA 2 cut(s) 516, 650
Ecl136II GAGCTC 1 cut(s) 524
EclXI CGGCCG 1 cut(s) 594
Eco105I TACGTA 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 3 cut(s) 208, 291, 397
Eco52I CGGCCG 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 524
Eco72I CACGTG 1 cut(s) 757
Eco88I CYCGRG 1 cut(s) 890
EcoICRI GAGCTC 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 526
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 1 cut(s) 562
FaiI YATR 5 cut(s) 546, 560, 614, 701, 975
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FatI CATG 1 cut(s) 558
FauI CCCGC 2 cut(s) 359, 821
Fnu4HI GCNGC 8 cut(s) 63, 123, 150, 261, 386, 594, 707, 743
FokI GGATG 4 cut(s) 640, 730, 737, 935
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 8 cut(s) 63, 123, 150, 261, 386, 594, 707, 743
FspBI CTAG 1 cut(s) 371
GluI GCNGC 8 cut(s) 63, 123, 150, 261, 386, 594, 707, 743
GsuI CTGGAG 2 cut(s) 164, 386
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 2 cut(s) 423, 596
HapII CCGG 8 cut(s) 153, 255, 519, 530, 576, 590, 597, 737
HgaI GACGC 2 cut(s) 290, 662
Hin1II CATG 1 cut(s) 562
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 7 cut(s) 158, 338, 448, 770, 781, 865, 977
HpaII CCGG 8 cut(s) 153, 255, 519, 530, 576, 590, 597, 737
HphI GGTGA 3 cut(s) 46, 238, 914
Hpy166II GTNNAC 6 cut(s) 145, 318, 397, 435, 609, 760
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 7 cut(s) 107, 162, 305, 519, 576, 778, 892
Hpy8I GTNNAC 6 cut(s) 145, 318, 397, 435, 609, 760
Hpy99I CGWCG 3 cut(s) 62, 544, 656
HpyAV CCTTC 9 cut(s) 20, 199, 244, 448, 463, 625, 826, 904, 958
HpyCH4III ACNGT 2 cut(s) 58, 94
HpyCH4IV ACGT 5 cut(s) 456, 539, 647, 756, 762
HpyCH4V TGCA 3 cut(s) 75, 360, 675
HpyF10VI GCNNNNNNNGC 2 cut(s) 886, 895
HpyF3I CTNAG 2 cut(s) 710, 873
HpySE526I ACGT 5 cut(s) 456, 539, 647, 756, 762
Hsp92II CATG 1 cut(s) 562
Kpn2I TCCGGA 2 cut(s) 518, 575
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Kzo9I GATC 4 cut(s) 112, 280, 532, 586
LmnI GCTCC 4 cut(s) 367, 502, 521, 529
Lsp1109I GCAGC 4 cut(s) 247, 372, 693, 729
MaeI CTAG 1 cut(s) 371
MaeII ACGT 5 cut(s) 456, 539, 647, 756, 762
MalI GATC 4 cut(s) 114, 282, 534, 588
MboI GATC 4 cut(s) 112, 280, 532, 586
MboII GAAGA 3 cut(s) 487, 494, 545
MflI RGATCY 2 cut(s) 532, 586
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 5 cut(s) 37, 97, 415, 547, 959
MluI ACGCGT 2 cut(s) 429, 641
MlyI GAGTC 3 cut(s) 332, 790, 874
MmeI TCCRAC 2 cut(s) 252, 825
MreI CGCCGGCG 1 cut(s) 152
MroI TCCGGA 2 cut(s) 518, 575
MroNI GCCGGC 1 cut(s) 152
MseI TTAA 1 cut(s) 885
MspI CCGG 8 cut(s) 153, 255, 519, 530, 576, 590, 597, 737
MspR9I CCNGG 2 cut(s) 140, 256
MteI GCGCNGCGC 1 cut(s) 386
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 2 cut(s) 886, 895
NaeI GCCGGC 1 cut(s) 154
NciI CCSGG 1 cut(s) 256
NdeII GATC 4 cut(s) 112, 280, 532, 586
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 1 cut(s) 562
NlaIV GGNNCC 2 cut(s) 293, 588
PaeR7I CTCGAG 1 cut(s) 890
PaqCI CACCTGC 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 303
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 4 cut(s) 158, 448, 770, 977
Pfl23II CGTACG 3 cut(s) 333, 644, 648
PkrI GCNGC 8 cut(s) 64, 124, 151, 262, 387, 595, 708, 744
PleI GAGTC 3 cut(s) 332, 789, 873
PmaCI CACGTG 1 cut(s) 757
PmlI CACGTG 1 cut(s) 757
PpsI GAGTC 3 cut(s) 332, 789, 873
Ppu21I YACGTR 2 cut(s) 648, 757
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 138
PspCI CACGTG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 138
PspLI CGTACG 3 cut(s) 333, 644, 648
PspN4I GGNNCC 2 cut(s) 293, 588
PspPI GGNCC 3 cut(s) 208, 291, 397
PsuI RGATCY 2 cut(s) 532, 586
RsaI GTAC 8 cut(s) 185, 273, 335, 436, 610, 646, 650, 761
RsaNI GTAC 8 cut(s) 184, 272, 334, 435, 609, 645, 649, 760
SacI GAGCTC 1 cut(s) 526
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 8 cut(s) 63, 123, 150, 261, 386, 594, 707, 743
Sau3AI GATC 4 cut(s) 112, 280, 532, 586
Sau96I GGNCC 3 cut(s) 208, 291, 397
SchI GAGTC 3 cut(s) 332, 790, 874
ScrFI CCNGG 2 cut(s) 140, 256
SduI GDGCHC 1 cut(s) 526
Sfr274I CTCGAG 1 cut(s) 890
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 3 cut(s) 208, 291, 397
SlaI CTCGAG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 778, 890
SmoI CTYRAG 2 cut(s) 778, 890
SnaBI TACGTA 1 cut(s) 648
Sse9I AATT 5 cut(s) 37, 97, 415, 547, 959
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 138, 254
TaaI ACNGT 2 cut(s) 58, 94
TaiI ACGT 5 cut(s) 459, 542, 650, 759, 765
TaqI TCGA 4 cut(s) 306, 375, 749, 891
TasI AATT 5 cut(s) 37, 97, 415, 547, 959
TatI WGTACW 1 cut(s) 608
TauI GCSGC 4 cut(s) 65, 125, 152, 596
TfiI GAWTC 4 cut(s) 158, 448, 770, 977
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 1 cut(s) 218
TseI GCWGC 4 cut(s) 260, 385, 706, 742
TspDTI ATGAA 4 cut(s) 232, 644, 939, 990
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 1 cut(s) 218
VpaK11BI GGWCC 3 cut(s) 208, 291, 397
XapI RAATTY 2 cut(s) 37, 415
XhoI CTCGAG 1 cut(s) 890
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.