Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0010967

Physical Location & Seq

Genomic Coordinates Forward (+)

49268427 .. 49271308

Gene Structure 2882 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G030130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGAAGAAGAAGATGATTTATATATACCTGTGGCAAAGCGGAGAGCGATGGCAGCGCAGAGGATTCTTCACCGGAAGGGGAATGCGTCCGGGCTTGAAGAGGAATCGGAGAAAGCCAAGGCTGATGAAGTGAAGCCTAGTCTTCTTGTGAAAGCTTCACAGTTGAAACGTGATGCTCCTGAGATTACTCAAGCCGAGCAGCTGGTGCAGCAAGAGAAAGAGATGATTGAGCATCTGTCTGATCGCAAGACCCTCATGTCGGTTCGTGAGTTGGCTAAGGGAATCACTTATACGGACCCTTTGCCCACAGGTTGGAAGCCCCCATTGCAGATTAGAAGGATGCCGACAAAGCACTGTGATTTGATTCGAAAGCAGTGGCACATCATAGTTAATGGCGAAGATATTCCACCGCCTATTAAGAATTTCAAGGATATGAGATTTCCTGAACCGATTTTGAAGATGTTGAAAACCAAGGGGATTGTTCAACCGACGCCCATTCAGGTGCAAGGTCTTCCGGTGATTTTGTCAGGGAGGGATATGATTGGTATTGCATTTACGGGTTCAGGAAAGACGTTGGTTTTTGTGTTGCCATTGATTATGATGGCAATGCAGGAGGAGATTATGATGCCAATTGTTCCAGGAGAAGGGCCTTTTGGATTGATCATTTGCCCATCCAGGGAGCTCGCAAGGCAGACTTATGAAGTAGTGGAACAATTTCTGGCTCCTATGAGAGAGGCTGGGTATCCAGAGATAAGGCCTTTGTTATGCATTGGTGGAGTGGAAATGAAGTCGCAGTTGGATATTGTGCGCAAAGGTGTTCATATAGTTGTTGCTACTCCCGGGAGGTTAAAGGATATGCTGGCAAAGAAAAAAATGAACCTTGATAATTGCAGATATCTAACTTTAGATGAGGCTGATAGATTGGTAGATTTGGGATTTGAAGATGACATAAGAGAAGTTTTTGACCATTTCAAAGCTCAACGACAGACACTCTTATTTTCAGCCACCATGCCTGCAAAAATTCAGAACTTTGCTAGAAGTGCTTTGGTTAAGCCTGTCACTGTCAATGTTGGACGAGCTGGAGCAGCAAATCTTGATGTGATTCAGGAGGTTGAGTATGTGAAGCAAGAAGCCAAGATTGTTTACCTTCTCGAATGTCTACAAAAGACCCCACCTCCAGTTCTGATATTCTGTGAAAATAAAGCTGATGTCGATGATATTCATGAATATCTTCTGTTAAAAGGAGTGGAAGCAGTGGCCATTCATGGAGGGAAGGATCAGGAAGAGAGAGAGTATGCCATTTCTTCCTTCAAGGCAGGGAAGAAAGATGTCTTAGTGGCAACTGATGTTGCCTCAAAAGGTTTGGATTTTCCTGATATTCAACATGTCATTAACTATGACATGCCAGCAGAAATCGAAAACTATGTTCACAGGATTGGACGAACTGGAAGATGTGGCAAGACTGGAATAGCAACAACATTTATAAACAAGAACCAGAGTGAGACTACACTTCTAGATTTGAAGCACCTATTGCAAGAGGCAAAACAGAGGATTCCACCTGTTTTGGCTGAGCTTAATGATCCAATGGAAGATGTTGAAGATATTACTGATGCAAGTGGAGTTAAAGGTTGTGCCTATTGTGGTGGGCTTGGTCATCGTATCCGTGATTGCCCAAAACTAGAGCAGCAGAAAAGCATGGCAATTTCCTCCTCTAGAAGAGATTATTACGGTTCTGGTGGTTACAGAGGAGAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C zf-CCHC

Protein Analysis

585

Amino Acids

65.54

Weight (kDa)

6.37

Isoelectric Point (pI)

45.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	164 - 343	1.4e-41	DEAD/DEAH box helicase
ResIII	PF04851	184 - 337	9.6e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	379 - 486	1.1e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33370 AT5G51280
fragaria_vesca	FvH4_3g15960 FvH4_6g29290
malus_domestica	MD09G1222100.v1.1 MD17G1213800.v1.1 MD17G1216300.v1.1
prunus_persica	Prupe.3G092400_v2.0.a1
pyrus_communis	pycom17g22090 pycom808g00170
rosa_chinensis	RchiOBHm_Chr2g0134471
rosa_laevigata	RLG00000019394
rosa_multiflora	Rmu_co8377539.1_g000001
rosa_roxburghii	Rroxscaffold_2G00110190
rosa_rugosa	Rorug02G0317300
rosa_samantha	Rh2AG375700 Rh2BG375700 Rh2CG353900 Rh2DG393000
rosa_wichuraiana	Rw2G030130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1484
AasI	GACNNNNNNGTC	1 cut(s)	256
Acc16I	TGCGCA	1 cut(s)	809
AccB7I	CCANNNNNTGG	1 cut(s)	312
AccI	GTMKAC	1 cut(s)	1159
AciI	CCGC	2 cut(s)	40, 410
AclWI	GGATC	2 cut(s)	1284, 1574
AcoI	YGGCCR	1 cut(s)	1257
AcsI	RAATTY	2 cut(s)	421, 1020
AcyI	GRCGYC	1 cut(s)	491
AfiI	CCNNNNNNNGG	4 cut(s)	259, 312, 644, 676
AflIII	ACRYGT	1 cut(s)	1384
AjnI	CCWGG	2 cut(s)	637, 674
AjuI	GAANNNNNNNTTGG	4 cut(s)	636, 668, 779, 811
AluBI	AGCT	7 cut(s)	155, 202, 682, 977, 1079, 1205, 1573
AluI	AGCT	7 cut(s)	155, 202, 682, 977, 1079, 1205, 1573
Alw21I	GWGCWC	1 cut(s)	684
Alw26I	GTCTC	1 cut(s)	1496
AlwI	GGATC	2 cut(s)	1284, 1574
Ama87I	CYCGRG	1 cut(s)	839
AoxI	GGCC	3 cut(s)	647, 755, 1257
ApeKI	GCWGC	5 cut(s)	53, 199, 208, 1085, 1684
ApoI	RAATTY	2 cut(s)	421, 1020
Asp700I	GAANNNNTTC	3 cut(s)	402, 714, 1230
AspLEI	GCGC	2 cut(s)	58, 810
AspS9I	GGNCC	2 cut(s)	295, 647
AsuC2I	CCSGG	3 cut(s)	91, 840, 841
AsuHPI	GGTGA	2 cut(s)	62, 529
AsuII	TTCGAA	1 cut(s)	367
AvaI	CYCGRG	1 cut(s)	839
AvaII	GGWCC	1 cut(s)	295
BalI	TGGCCA	1 cut(s)	1259
BanII	GRGCYC	1 cut(s)	684
BbsI	GAAGAC	2 cut(s)	134, 503
Bbv12I	GWGCWC	1 cut(s)	684
BbvI	GCAGC	5 cut(s)	65, 211, 220, 1097, 1696
BccI	CCATC	3 cut(s)	43, 595, 679
BciT130I	CCWGG	2 cut(s)	639, 676
BciVI	GTATCC	2 cut(s)	753, 1670
BclI	TGATCA	1 cut(s)	660
BcnI	CCSGG	3 cut(s)	91, 840, 841
BcoDI	GTCTC	1 cut(s)	1496
BfaI	CTAG	5 cut(s)	138, 1035, 1514, 1679, 1713
BfuI	GTATCC	2 cut(s)	753, 1670
BisI	GCNGC	5 cut(s)	54, 200, 209, 1086, 1685
BlpI	GCTNAGC	1 cut(s)	1569
BlsI	GCNGC	5 cut(s)	55, 201, 210, 1087, 1686
Bme1390I	CCNGG	5 cut(s)	91, 639, 676, 840, 841
Bme18I	GGWCC	1 cut(s)	295
BmeT110I	CYCGRG	1 cut(s)	839
BmgT120I	GGNCC	2 cut(s)	295, 647
BmiI	GGNNCC	2 cut(s)	297, 723
BmrFI	CCNGG	5 cut(s)	91, 639, 676, 840, 841
BmsI	GCATC	5 cut(s)	163, 241, 330, 615, 1600
BpiI	GAAGAC	2 cut(s)	134, 503
BpmI	CTGGAG	2 cut(s)	1101, 1161
Bpu10I	CCTNAGC	1 cut(s)	276
Bpu1102I	GCTNAGC	1 cut(s)	1569
Bpu14I	TTCGAA	1 cut(s)	367
BpuEI	CTTGAG	1 cut(s)	174
BpuMI	CCSGG	3 cut(s)	91, 840, 841
BsaHI	GRCGYC	1 cut(s)	491
BsaJI	CCNNGG	4 cut(s)	117, 471, 675, 839
BsaWI	WCCGGW	2 cut(s)	72, 514
BsaXI	ACNNNNNCTCC	4 cut(s)	1100, 1130, 1159, 1189
Bsc4I	CCNNNNNNNGG	4 cut(s)	259, 312, 644, 676
Bse1I	ACTGG	3 cut(s)	1178, 1450, 1468
Bse3DI	GCAATG	2 cut(s)	323, 612
BseBI	CCWGG	2 cut(s)	639, 676
BseDI	CCNNGG	4 cut(s)	117, 471, 675, 839
BseGI	GGATG	2 cut(s)	345, 671
BseLI	CCNNNNNNNGG	4 cut(s)	259, 312, 644, 676
BseMI	GCAATG	2 cut(s)	323, 612
BseMII	CTCAG	2 cut(s)	171, 1560
BseNI	ACTGG	3 cut(s)	1178, 1450, 1468
BseRI	GAGGAG	2 cut(s)	629, 1699
BseXI	GCAGC	5 cut(s)	65, 211, 220, 1097, 1696
BseYI	CCCAGC	1 cut(s)	737
BsgI	GTGCAG	1 cut(s)	227
BshFI	GGCC	3 cut(s)	649, 757, 1259
BsiHKAI	GWGCWC	1 cut(s)	684
BsiHKCI	CYCGRG	1 cut(s)	839
BsiSI	CCGG	4 cut(s)	73, 90, 515, 840
BslI	CCNNNNNNNGG	4 cut(s)	259, 312, 644, 676
BsmAI	GTCTC	1 cut(s)	1496
BsmI	GAATGC	1 cut(s)	88
BsnI	GGCC	3 cut(s)	649, 757, 1259
BsoBI	CYCGRG	1 cut(s)	839
Bsp119I	TTCGAA	1 cut(s)	367
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp143I	GATC	4 cut(s)	241, 660, 1276, 1579
Bsp1720I	GCTNAGC	1 cut(s)	1569
BspACI	CCGC	2 cut(s)	40, 410
BspANI	GGCC	3 cut(s)	649, 757, 1259
BspCNI	CTCAG	2 cut(s)	172, 1561
BspHI	TCATGA	1 cut(s)	1222
BspLI	GGNNCC	2 cut(s)	297, 723
BspPI	GGATC	2 cut(s)	1284, 1574
BspT104I	TTCGAA	1 cut(s)	367
BsrDI	GCAATG	2 cut(s)	323, 612
BsrI	ACTGG	3 cut(s)	1178, 1450, 1468
BssECI	CCNNGG	4 cut(s)	117, 471, 675, 839
BssMI	GATC	4 cut(s)	241, 660, 1276, 1579
BssNI	GRCGYC	1 cut(s)	491
BssT1I	CCWWGG	2 cut(s)	117, 471
Bst2UI	CCWGG	2 cut(s)	639, 676
Bst4CI	ACNGT	4 cut(s)	162, 356, 1063, 1730
Bst6I	CTCTTC	3 cut(s)	93, 1278, 1711
BstACI	GRCGYC	1 cut(s)	491
BstAPI	GCANNNNNTGC	2 cut(s)	205, 1531
BstBI	TTCGAA	1 cut(s)	367
BstC8I	GCNNGC	4 cut(s)	684, 861, 1014, 1407
BstDEI	CTNAG	4 cut(s)	180, 276, 1333, 1569
BstF5I	GGATG	2 cut(s)	345, 671
BstHHI	GCGC	2 cut(s)	58, 810
BstKTI	GATC	4 cut(s)	244, 663, 1279, 1582
BstMAI	GTCTC	1 cut(s)	1496
BstMBI	GATC	4 cut(s)	241, 660, 1276, 1579
BstMWI	GCNNNNNNNGC	9 cut(s)	53, 205, 208, 325, 349, 688, 1040, 1085, 1531
BstNI	CCWGG	2 cut(s)	639, 676
BstNSI	RCATGY	2 cut(s)	1388, 1405
BstSCI	CCNGG	5 cut(s)	89, 637, 674, 838, 839
BstV1I	GCAGC	5 cut(s)	65, 211, 220, 1097, 1696
BstV2I	GAAGAC	2 cut(s)	134, 503
BsuI	GTATCC	2 cut(s)	753, 1670
BsuRI	GGCC	3 cut(s)	649, 757, 1259
BtgZI	GCGATG	1 cut(s)	62
BtsCI	GGATG	2 cut(s)	345, 671
BtsI	GCAGTG	2 cut(s)	380, 1260
BtsIMutI	CAGTG	4 cut(s)	352, 380, 1059, 1260
Cac8I	GCNNGC	4 cut(s)	684, 861, 1014, 1407
CciI	TCATGA	1 cut(s)	1222
CfoI	GCGC	2 cut(s)	58, 810
Cfr13I	GGNCC	2 cut(s)	295, 647
Cfr9I	CCCGGG	1 cut(s)	839
CseI	GACGC	2 cut(s)	75, 499
CspCI	CAANNNNNGTGG	2 cut(s)	1545, 1580
CviAII	CATG	7 cut(s)	256, 1009, 1223, 1265, 1385, 1402, 1696
DdeI	CTNAG	4 cut(s)	180, 276, 1333, 1569
DpnI	GATC	4 cut(s)	243, 662, 1278, 1581
DpnII	GATC	4 cut(s)	241, 660, 1276, 1579
DrdI	GACNNNNNNGTC	1 cut(s)	256
DseDI	GACNNNNNNGTC	1 cut(s)	256
EaeI	YGGCCR	1 cut(s)	1257
Eam1104I	CTCTTC	3 cut(s)	93, 1278, 1711
EarI	CTCTTC	3 cut(s)	93, 1278, 1711
Ecl136II	GAGCTC	1 cut(s)	682
Eco130I	CCWWGG	2 cut(s)	117, 471
Eco147I	AGGCCT	1 cut(s)	757
Eco24I	GRGCYC	1 cut(s)	684
Eco32I	GATATC	1 cut(s)	896
Eco47I	GGWCC	1 cut(s)	295
Eco53kI	GAGCTC	1 cut(s)	682
Eco88I	CYCGRG	1 cut(s)	839
EcoICRI	GAGCTC	1 cut(s)	682
EcoO109I	RGGNCCY	1 cut(s)	647
EcoRII	CCWGG	2 cut(s)	637, 674
EcoRV	GATATC	1 cut(s)	896
EcoT14I	CCWWGG	2 cut(s)	117, 471
EcoT22I	ATGCAT	1 cut(s)	770
EcoT38I	GRGCYC	1 cut(s)	684
ErhI	CCWWGG	2 cut(s)	117, 471
FaeI	CATG	7 cut(s)	259, 1012, 1226, 1268, 1388, 1405, 1699
FalI	AAGNNNNNCTT	2 cut(s)	679, 711
FatI	CATG	7 cut(s)	255, 1008, 1222, 1264, 1384, 1401, 1695
FbaI	TGATCA	1 cut(s)	660
FblI	GTMKAC	1 cut(s)	1159
Fnu4HI	GCNGC	5 cut(s)	54, 200, 209, 1086, 1685
FokI	GGATG	2 cut(s)	352, 658
FriOI	GRGCYC	1 cut(s)	684
Fsp4HI	GCNGC	5 cut(s)	54, 200, 209, 1086, 1685
FspBI	CTAG	5 cut(s)	138, 1035, 1514, 1679, 1713
FspI	TGCGCA	1 cut(s)	809
GlaI	GCGC	2 cut(s)	57, 809
GluI	GCNGC	5 cut(s)	54, 200, 209, 1086, 1685
GsaI	CCCAGC	1 cut(s)	741
GsuI	CTGGAG	2 cut(s)	1101, 1161
HaeIII	GGCC	3 cut(s)	649, 757, 1259
HapII	CCGG	4 cut(s)	73, 90, 515, 840
HgaI	GACGC	2 cut(s)	75, 499
HhaI	GCGC	2 cut(s)	58, 810
Hin1I	GRCGYC	1 cut(s)	491
Hin1II	CATG	7 cut(s)	259, 1012, 1226, 1268, 1388, 1405, 1699
Hin6I	GCGC	2 cut(s)	56, 808
HinP1I	GCGC	2 cut(s)	56, 808
HindIII	AAGCTT	1 cut(s)	153
HinfI	GANTC	6 cut(s)	64, 104, 282, 364, 1102, 1552
HpaII	CCGG	4 cut(s)	73, 90, 515, 840
HphI	GGTGA	2 cut(s)	62, 529
Hpy166II	GTNNAC	3 cut(s)	1144, 1160, 1429
Hpy188I	TCNGA	4 cut(s)	109, 241, 1026, 1185
Hpy8I	GTNNAC	3 cut(s)	1144, 1160, 1429
Hpy99I	CGWCG	1 cut(s)	493
HpyAV	CCTTC	6 cut(s)	70, 330, 638, 1157, 1267, 1318
HpyCH4III	ACNGT	4 cut(s)	162, 356, 1063, 1730
HpyCH4IV	ACGT	2 cut(s)	169, 572
HpyF10VI	GCNNNNNNNGC	9 cut(s)	53, 205, 208, 325, 349, 688, 1040, 1085, 1531
HpyF3I	CTNAG	4 cut(s)	180, 276, 1333, 1569
HpySE526I	ACGT	2 cut(s)	169, 572
Hsp92I	GRCGYC	1 cut(s)	491
Hsp92II	CATG	7 cut(s)	259, 1012, 1226, 1268, 1388, 1405, 1699
HspAI	GCGC	2 cut(s)	56, 808
Ksp22I	TGATCA	1 cut(s)	660
Kzo9I	GATC	4 cut(s)	241, 660, 1276, 1579
LmnI	GCTCC	4 cut(s)	181, 679, 727, 1082
Lsp1109I	GCAGC	5 cut(s)	65, 211, 220, 1097, 1696
LweI	GCATC	5 cut(s)	163, 241, 330, 615, 1600
MaeI	CTAG	5 cut(s)	138, 1035, 1514, 1679, 1713
MaeII	ACGT	2 cut(s)	169, 572
MaeIII	GTNAC	2 cut(s)	1057, 1739
MalI	GATC	4 cut(s)	243, 662, 1278, 1581
MboI	GATC	4 cut(s)	241, 660, 1276, 1579
MfeI	CAATTG	1 cut(s)	630
MhlI	GDGCHC	1 cut(s)	684
MlsI	TGGCCA	1 cut(s)	1259
MluCI	AATT	6 cut(s)	421, 630, 713, 886, 1020, 1701
MluNI	TGGCCA	1 cut(s)	1259
MmeI	TCCRAC	3 cut(s)	293, 777, 1051
Mox20I	TGGCCA	1 cut(s)	1259
Mph1103I	ATGCAT	1 cut(s)	770
MroXI	GAANNNNTTC	3 cut(s)	402, 714, 1230
MscI	TGGCCA	1 cut(s)	1259
MseI	TTAA	8 cut(s)	390, 417, 848, 1050, 1238, 1392, 1575, 1623
MslI	CAYNNNNRTG	1 cut(s)	500
Msp20I	TGGCCA	1 cut(s)	1259
MspA1I	CMGCKG	1 cut(s)	202
MspI	CCGG	4 cut(s)	73, 90, 515, 840
MspR9I	CCNGG	5 cut(s)	91, 639, 676, 840, 841
MunI	CAATTG	1 cut(s)	630
Mva1269I	GAATGC	1 cut(s)	88
MvaI	CCWGG	2 cut(s)	639, 676
MwoI	GCNNNNNNNGC	9 cut(s)	53, 205, 208, 325, 349, 688, 1040, 1085, 1531
NciI	CCSGG	3 cut(s)	91, 840, 841
NdeII	GATC	4 cut(s)	241, 660, 1276, 1579
NlaIII	CATG	7 cut(s)	259, 1012, 1226, 1268, 1388, 1405, 1699
NlaIV	GGNNCC	2 cut(s)	297, 723
NmeAIII	GCCGAG	1 cut(s)	220
NmuCI	GTSAC	1 cut(s)	1057
NsbI	TGCGCA	1 cut(s)	809
NsiI	ATGCAT	1 cut(s)	770
NspI	RCATGY	2 cut(s)	1388, 1405
NspV	TTCGAA	1 cut(s)	367
PagI	TCATGA	1 cut(s)	1222
PceI	AGGCCT	1 cut(s)	757
PciI	ACATGT	1 cut(s)	1384
PctI	GAATGC	1 cut(s)	88
PdmI	GAANNNNTTC	3 cut(s)	402, 714, 1230
PfeI	GAWTC	6 cut(s)	64, 104, 282, 364, 1102, 1552
PflMI	CCANNNNNTGG	1 cut(s)	312
PfoI	TCCNGGA	1 cut(s)	637
PkrI	GCNGC	5 cut(s)	55, 201, 210, 1087, 1686
PscI	ACATGT	1 cut(s)	1384
PsiI	TTATAA	1 cut(s)	1484
Psp124BI	GAGCTC	1 cut(s)	684
Psp6I	CCWGG	2 cut(s)	637, 674
PspFI	CCCAGC	1 cut(s)	737
PspGI	CCWGG	2 cut(s)	637, 674
PspN4I	GGNNCC	2 cut(s)	297, 723
PspPI	GGNCC	2 cut(s)	295, 647
PvuII	CAGCTG	1 cut(s)	202
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	1 cut(s)	684
SaqAI	TTAA	8 cut(s)	390, 417, 848, 1050, 1238, 1392, 1575, 1623
SatI	GCNGC	5 cut(s)	54, 200, 209, 1086, 1685
Sau3AI	GATC	4 cut(s)	241, 660, 1276, 1579
Sau96I	GGNCC	2 cut(s)	295, 647
ScrFI	CCNGG	5 cut(s)	91, 639, 676, 840, 841
SduI	GDGCHC	1 cut(s)	684
SfaNI	GCATC	5 cut(s)	163, 241, 330, 615, 1600
SfuI	TTCGAA	1 cut(s)	367
SinI	GGWCC	1 cut(s)	295
SmaI	CCCGGG	1 cut(s)	841
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	1 cut(s)	189
SmoI	CTYRAG	1 cut(s)	189
Sse9I	AATT	6 cut(s)	421, 630, 713, 886, 1020, 1701
SseBI	AGGCCT	1 cut(s)	757
SsiI	CCGC	2 cut(s)	40, 410
SspMI	CTAG	5 cut(s)	138, 1035, 1514, 1679, 1713
SstI	GAGCTC	1 cut(s)	684
StuI	AGGCCT	1 cut(s)	757
StyD4I	CCNGG	5 cut(s)	89, 637, 674, 838, 839
StyI	CCWWGG	2 cut(s)	117, 471
TaaI	ACNGT	4 cut(s)	162, 356, 1063, 1730
TaiI	ACGT	2 cut(s)	172, 575
TaqI	TCGA	4 cut(s)	367, 1152, 1212, 1416
TasI	AATT	6 cut(s)	421, 630, 713, 886, 1020, 1701
TfiI	GAWTC	6 cut(s)	64, 104, 282, 364, 1102, 1552
Tru1I	TTAA	8 cut(s)	390, 417, 848, 1050, 1238, 1392, 1575, 1623
Tru9I	TTAA	8 cut(s)	390, 417, 848, 1050, 1238, 1392, 1575, 1623
TscAI	CASTG	4 cut(s)	359, 380, 1066, 1260
TseFI	GTSAC	1 cut(s)	1057
TseI	GCWGC	5 cut(s)	53, 199, 208, 1085, 1684
Tsp45I	GTSAC	1 cut(s)	1057
TspDTI	ATGAA	8 cut(s)	141, 714, 800, 809, 890, 1211, 1239, 1253
TspGWI	ACGGA	2 cut(s)	308, 1652
TspMI	CCCGGG	1 cut(s)	839
TspRI	CASTG	4 cut(s)	359, 380, 1066, 1260
Van91I	CCANNNNNTGG	1 cut(s)	312
VpaK11BI	GGWCC	1 cut(s)	295
XapI	RAATTY	2 cut(s)	421, 1020
XbaI	TCTAGA	2 cut(s)	1513, 1712
XceI	RCATGY	2 cut(s)	1388, 1405
XmaI	CCCGGG	1 cut(s)	839
XmiI	GTMKAC	1 cut(s)	1159
XmnI	GAANNNNTTC	3 cut(s)	402, 714, 1230
XspI	CTAG	5 cut(s)	138, 1035, 1514, 1679, 1713
Zsp2I	ATGCAT	1 cut(s)	770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.