Rw2G032520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018666
Physical Location & Seq
Forward (+)
53488883 .. 53492352
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032520.1

Sequence Viewer

Length: 825 bp
ATGTTGGTGCGAGCCGGTCTGAGTCTGCTGATCAAGCGATGGGGTCGAATGGTTTCAGATCCGGTGACGGCAAAGGGGGAGAGGGCTGTTTTGGGGGTTGTTAATCCCGATCCGGTTGTTGGAGCTCGATCGGAGGAGGAGAAGAAGAGGGGCAAGGAGTACAAAGCAGAAGATCGAGGAGGAGGGCCAGGAGCTCGAAGTACAAACTACGTACAAAGGATGAGCTCAACTGGTGGGGAGGAAGCAAATGATGATGGGAATGGGATGCAGTTAGGCAGCGAAGCTGACGACCTAGCAGGCGAGTATCTTCACCACAAGACAATCAACGACCGTATCTTGTATCTTATCAACATGTTCGGCGTCTGTGTCGTGCTCAAGAACGGAGGAGGTTTCGGAAGTAGTCCGTCTCTCTTTGTCACAGACTTCCTTACCACGCGTGCCCTTCTTCTGGCTTTGTCCCTCTACACCGTCTCACTGGCTACCGCTGACGCAAAAACTCACTCCAATGTCGAGTCCGATCCGGATGAAGCTGAATTCACGAATAACATTACCGTCGTCTGTGGCACTCTAGTCTTGACTTTACAGTTGTTTCTTCTTGTTCCAGCTTTTGGGTGGTTAGCCCTCGTCTCTTGGACCATTAATTTGGTGAGGGTTTTAACAAAATCGTGGTGCTACTTAAAGAGATTGTATCAAACTAAAGTTGCAGCAGTAGTTGACGAATTCAGGACTTTGAAGGAGCTCATCGTGAGGTGTCTTGCCGGATTGAAGACTTTGAGGGATGGCGACACCACCCACGAAACAGAGGAGGAGAATGGACGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

29.95

Weight (kDa)

5.89

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018666)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019738
rosa_multiflora Rmu_co8439821.1_g000001 Rmu_sc0002475.1_g000003 Rmu_sc0006186.1_g000001
rosa_roxburghii Rroxscaffold_2G00106320
rosa_samantha Rh2AG399600 Rh2BG408600 Rh2CG386000 Rh2DG419400
rosa_wichuraiana Rw2G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 820
AccB7I CCANNNNNTGG 1 cut(s) 608
AccII CGCG 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 520
AciI CCGC 1 cut(s) 483
AclWI GGATC 3 cut(s) 53, 104, 512
AcsI RAATTY 2 cut(s) 533, 719
AcyI GRCGYC 2 cut(s) 360, 817
AfaI GTAC 3 cut(s) 161, 202, 213
AfiI CCNNNNNNNGG 3 cut(s) 119, 448, 608
AflIII ACRYGT 2 cut(s) 351, 434
AgsI TTSAA 2 cut(s) 733, 766
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 7 cut(s) 125, 194, 225, 284, 530, 605, 739
AluI AGCT 7 cut(s) 125, 194, 225, 284, 530, 605, 739
Alw21I GWGCWC 5 cut(s) 127, 196, 227, 375, 741
Alw26I GTCTC 3 cut(s) 411, 475, 631
AlwI GGATC 3 cut(s) 53, 104, 512
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 276, 704
ApoI RAATTY 2 cut(s) 533, 719
ArsI GACNNNNNNTTYG 2 cut(s) 625, 657
AseI ATTAAT 1 cut(s) 639
Asp700I GAANNNNTTC 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 185, 633
AsuHPI GGTGA 3 cut(s) 76, 302, 658
AvaII GGWCC 1 cut(s) 633
BaeGI GKGCMC 1 cut(s) 442
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BanII GRGCYC 4 cut(s) 127, 196, 227, 741
BbsI GAAGAC 1 cut(s) 773
Bbv12I GWGCWC 5 cut(s) 127, 196, 227, 375, 741
BbvI GCAGC 2 cut(s) 288, 716
BccI CCATC 3 cut(s) 33, 248, 773
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 1 cut(s) 189
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 3 cut(s) 411, 475, 631
BfaI CTAG 2 cut(s) 293, 569
BisI GCNGC 2 cut(s) 277, 705
BlsI GCNGC 2 cut(s) 278, 706
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 633
BmgT120I GGNCC 2 cut(s) 185, 633
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 211
BsaHI GRCGYC 2 cut(s) 360, 817
BsaWI WCCGGW 3 cut(s) 61, 112, 520
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 448, 608
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 235, 480
BseAI TCCGGA 1 cut(s) 520
BseBI CCWGG 1 cut(s) 189
BseGI GGATG 4 cut(s) 225, 270, 529, 784
BseLI CCNNNNNNNGG 3 cut(s) 119, 448, 608
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 2 cut(s) 235, 480
BseRI GAGGAG 7 cut(s) 149, 152, 192, 195, 399, 818, 821
BseSI GKGCMC 1 cut(s) 442
BseXI GCAGC 2 cut(s) 288, 716
Bsh1236I CGCG 1 cut(s) 436
Bsh1285I CGRYCG 2 cut(s) 131, 331
BshFI GGCC 1 cut(s) 187
BsiEI CGRYCG 2 cut(s) 131, 331
BsiHKAI GWGCWC 5 cut(s) 127, 196, 227, 375, 741
BsiSI CCGG 5 cut(s) 15, 62, 113, 521, 759
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 3 cut(s) 119, 448, 608
BsmAI GTCTC 3 cut(s) 411, 475, 631
BsmBI CGTCTC 3 cut(s) 411, 475, 631
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 187
Bsp1286I GDGCHC 6 cut(s) 127, 196, 227, 375, 442, 741
Bsp13I TCCGGA 1 cut(s) 520
Bsp143I GATC 6 cut(s) 30, 58, 109, 128, 172, 517
BspACI CCGC 1 cut(s) 483
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 12
BspEI TCCGGA 1 cut(s) 520
BspFNI CGCG 1 cut(s) 436
BspPI GGATC 3 cut(s) 53, 104, 512
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 2 cut(s) 235, 480
BssAI RCCGGY 1 cut(s) 14
BssMI GATC 6 cut(s) 30, 58, 109, 128, 172, 517
BssNI GRCGYC 2 cut(s) 360, 817
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 4 cut(s) 332, 469, 553, 585
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 2 cut(s) 360, 817
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 3 cut(s) 12, 298, 438
BstDEI CTNAG 1 cut(s) 20
BstF5I GGATG 4 cut(s) 225, 270, 529, 784
BstFNI CGCG 1 cut(s) 436
BstKTI GATC 6 cut(s) 33, 61, 112, 131, 175, 520
BstMAI GTCTC 3 cut(s) 411, 475, 631
BstMBI GATC 6 cut(s) 30, 58, 109, 128, 172, 517
BstMCI CGRYCG 2 cut(s) 131, 331
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 442
BstSNI TACGTA 1 cut(s) 211
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 288, 716
BstV2I GAAGAC 1 cut(s) 773
BstX2I RGATCY 1 cut(s) 58
BstXI CCANNNNNNTGG 1 cut(s) 643
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 187
BtgZI GCGATG 1 cut(s) 52
BtsCI GGATG 4 cut(s) 225, 270, 529, 784
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 3 cut(s) 12, 298, 438
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 185, 633
CseI GACGC 2 cut(s) 349, 497
Csp6I GTAC 3 cut(s) 160, 201, 212
CviAII CATG 1 cut(s) 352
CviQI GTAC 3 cut(s) 160, 201, 212
DdeI CTNAG 1 cut(s) 20
DpnI GATC 6 cut(s) 32, 60, 111, 130, 174, 519
DpnII GATC 6 cut(s) 30, 58, 109, 128, 172, 517
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 4 cut(s) 125, 194, 225, 739
Eco105I TACGTA 1 cut(s) 211
Eco24I GRGCYC 4 cut(s) 127, 196, 227, 741
Eco47I GGWCC 1 cut(s) 633
Eco53kI GAGCTC 4 cut(s) 125, 194, 225, 739
EcoICRI GAGCTC 4 cut(s) 125, 194, 225, 739
EcoRI GAATTC 2 cut(s) 533, 719
EcoRII CCWGG 1 cut(s) 187
EcoT38I GRGCYC 4 cut(s) 127, 196, 227, 741
Esp3I CGTCTC 3 cut(s) 411, 475, 631
FaeI CATG 1 cut(s) 355
FaiI YATR 1 cut(s) 353
FaqI GGGAC 1 cut(s) 442
FatI CATG 1 cut(s) 351
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 2 cut(s) 277, 705
FokI GGATG 4 cut(s) 232, 277, 536, 791
FriOI GRGCYC 4 cut(s) 127, 196, 227, 741
Fsp4HI GCNGC 2 cut(s) 277, 705
FspBI CTAG 2 cut(s) 293, 569
GluI GCNGC 2 cut(s) 277, 705
HaeIII GGCC 1 cut(s) 187
HapII CCGG 5 cut(s) 15, 62, 113, 521, 759
HgaI GACGC 2 cut(s) 349, 497
Hin1I GRCGYC 2 cut(s) 360, 817
Hin1II CATG 1 cut(s) 355
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HinfI GANTC 2 cut(s) 22, 512
HpaII CCGG 5 cut(s) 15, 62, 113, 521, 759
HphI GGTGA 3 cut(s) 76, 302, 658
Hpy166II GTNNAC 1 cut(s) 715
Hpy188I TCNGA 5 cut(s) 21, 58, 133, 395, 517
Hpy188III TCNNGA 7 cut(s) 107, 376, 521, 538, 574, 724, 745
Hpy8I GTNNAC 1 cut(s) 715
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 2 cut(s) 452, 727
HpyCH4III ACNGT 4 cut(s) 332, 469, 553, 585
HpyCH4IV ACGT 2 cut(s) 210, 817
HpyCH4V TGCA 2 cut(s) 268, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 2 cut(s) 210, 817
Hsp92I GRCGYC 2 cut(s) 360, 817
Hsp92II CATG 1 cut(s) 355
Kpn2I TCCGGA 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 6 cut(s) 30, 58, 109, 128, 172, 517
LmnI GCTCC 3 cut(s) 122, 191, 736
Lsp1109I GCAGC 2 cut(s) 288, 716
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 293, 569
MaeII ACGT 2 cut(s) 210, 817
MaeIII GTNAC 2 cut(s) 64, 415
MalI GATC 6 cut(s) 32, 60, 111, 130, 174, 519
MboI GATC 6 cut(s) 30, 58, 109, 128, 172, 517
MboII GAAGA 7 cut(s) 154, 157, 182, 299, 437, 584, 778
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 6 cut(s) 127, 196, 227, 375, 442, 741
MluCI AATT 3 cut(s) 533, 640, 719
MluI ACGCGT 1 cut(s) 434
MlyI GAGTC 2 cut(s) 31, 521
MmeI TCCRAC 1 cut(s) 100
MroI TCCGGA 1 cut(s) 520
MroXI GAANNNNTTC 1 cut(s) 52
MseI TTAA 4 cut(s) 102, 639, 656, 677
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 5 cut(s) 15, 62, 113, 521, 759
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 1 cut(s) 34
NdeII GATC 6 cut(s) 30, 58, 109, 128, 172, 517
NlaIII CATG 1 cut(s) 355
NmuCI GTSAC 2 cut(s) 64, 415
NspI RCATGY 1 cut(s) 355
PciI ACATGT 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 2 cut(s) 278, 706
Ple19I CGATCG 1 cut(s) 131
PleI GAGTC 2 cut(s) 30, 520
PpsI GAGTC 2 cut(s) 30, 520
Ppu21I YACGTR 1 cut(s) 211
PscI ACATGT 1 cut(s) 351
PshBI ATTAAT 1 cut(s) 639
Psp124BI GAGCTC 4 cut(s) 127, 196, 227, 741
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 2 cut(s) 185, 633
PsuI RGATCY 1 cut(s) 58
PvuI CGATCG 1 cut(s) 131
RsaI GTAC 3 cut(s) 161, 202, 213
RsaNI GTAC 3 cut(s) 160, 201, 212
RseI CAYNNNNRTG 1 cut(s) 504
SacI GAGCTC 4 cut(s) 127, 196, 227, 741
SaqAI TTAA 4 cut(s) 102, 639, 656, 677
SatI GCNGC 2 cut(s) 277, 705
Sau3AI GATC 6 cut(s) 30, 58, 109, 128, 172, 517
Sau96I GGNCC 2 cut(s) 185, 633
SchI GAGTC 2 cut(s) 31, 521
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 6 cut(s) 127, 196, 227, 375, 442, 741
SfaNI GCATC 1 cut(s) 255
SinI GGWCC 1 cut(s) 633
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SnaBI TACGTA 1 cut(s) 211
Sse9I AATT 3 cut(s) 533, 640, 719
SsiI CCGC 1 cut(s) 483
SspMI CTAG 2 cut(s) 293, 569
SstI GAGCTC 4 cut(s) 127, 196, 227, 741
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 4 cut(s) 332, 469, 553, 585
TaiI ACGT 2 cut(s) 213, 820
TaqI TCGA 5 cut(s) 46, 127, 175, 196, 510
TasI AATT 3 cut(s) 533, 640, 719
TatI WGTACW 2 cut(s) 159, 200
Tru1I TTAA 4 cut(s) 102, 639, 656, 677
Tru9I TTAA 4 cut(s) 102, 639, 656, 677
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 2 cut(s) 64, 415
TseI GCWGC 2 cut(s) 276, 704
Tsp45I GTSAC 2 cut(s) 64, 415
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 2 cut(s) 393, 396
TspRI CASTG 1 cut(s) 480
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 633
VspI ATTAAT 1 cut(s) 639
XapI RAATTY 2 cut(s) 533, 719
XceI RCATGY 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 52
XspI CTAG 2 cut(s) 293, 569
ZraI GACGTC 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.