Rw2G052710

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017786
Physical Location & Seq
Forward (+)
80629932 .. 80633480
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052710.1

Sequence Viewer

Length: 354 bp
ATGGCCCCAGAGTATCTTGTTCAAGGGCAACTTATAGAGAAAGCAGACATTTATAGCTTCAGAGTGCTAGTTCTCGAGATTGTGTCTGGTATGAAGAATAGCACCTTCAAACAGGCGGACACTGACTTTGATTCACTACTACACAAAGTTTGGAAACACTACAGATCAAACGAGTTGGCCGACGTGGTTGATCCTTGCTTGAGAGATCATGAGTTGCCAGCAAAAGAGGTATCAAATGTTCTTCAAATTGGACTTCTGTGCACACAGGCCTCAGTTGCCTTGAGACCATCTATGGCTGAAGAAAGCATGCTTGGAGGTCAGGTCAGTATGCAGGAAGCAGAAATGCAGAATTAG

Protein Analysis

117

Amino Acids

13.13

Weight (kDa)

4.8

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 99 1e-05 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 116
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 177
AcuI CTGAAG 2 cut(s) 43, 318
AgsI TTSAA 3 cut(s) 23, 109, 245
AjiI CACGTC 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 277
Alw44I GTGCAC 1 cut(s) 259
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 3 cut(s) 3, 177, 267
ApaLI GTGCAC 1 cut(s) 259
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspS9I GGNCC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 263
BccI CCATC 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 74
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 220, 301
BsaI GGTCTC 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
BseMII CTCAG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 263
BshFI GGCC 3 cut(s) 5, 179, 269
BsiHKAI GWGCWC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 277
BsnI GGCC 3 cut(s) 5, 179, 269
Bso31I GGTCTC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 3 cut(s) 164, 190, 205
BspACI CCGC 1 cut(s) 116
BspANI GGCC 3 cut(s) 5, 179, 269
BspCNI CTCAG 1 cut(s) 284
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 277
BssMI GATC 3 cut(s) 164, 190, 205
BstC8I GCNNGC 2 cut(s) 219, 308
BstDEI CTNAG 1 cut(s) 271
BstKTI GATC 3 cut(s) 167, 193, 208
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 3 cut(s) 164, 190, 205
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 263
BsuRI GGCC 3 cut(s) 5, 179, 269
BtrI CACGTC 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 219, 308
CciI TCATGA 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 2 cut(s) 209, 307
CviJI RGCY 5 cut(s) 5, 57, 179, 269, 296
CviKI_1 RGCY 5 cut(s) 5, 57, 179, 269, 296
DdeI CTNAG 1 cut(s) 271
DpnI GATC 3 cut(s) 166, 192, 207
DpnII GATC 3 cut(s) 164, 190, 205
EaeI YGGCCR 1 cut(s) 177
EciI GGCGGA 1 cut(s) 131
Eco147I AGGCCT 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 277
Eco57I CTGAAG 2 cut(s) 43, 318
Eco88I CYCGRG 1 cut(s) 74
FaeI CATG 2 cut(s) 212, 310
FaiI YATR 7 cut(s) 35, 54, 92, 210, 293, 308, 329
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FatI CATG 2 cut(s) 208, 306
FspBI CTAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 5, 179, 269
Hin1II CATG 2 cut(s) 212, 310
HinfI GANTC 1 cut(s) 131
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 3 cut(s) 74, 76, 209
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 115
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 3 cut(s) 261, 331, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 2 cut(s) 212, 310
Kzo9I GATC 3 cut(s) 164, 190, 205
LpnPI CCDG 7 cut(s) 21, 72, 98, 231, 251, 305, 317
MaeI CTAG 1 cut(s) 68
MaeII ACGT 1 cut(s) 183
MalI GATC 3 cut(s) 166, 192, 207
MboI GATC 3 cut(s) 164, 190, 205
MboII GAAGA 3 cut(s) 106, 233, 311
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 2 cut(s) 246, 349
MnlI CCTC 3 cut(s) 220, 280, 308
MwoI GCNNNNNNNGC 1 cut(s) 275
NdeII GATC 3 cut(s) 164, 190, 205
NlaIII CATG 2 cut(s) 212, 310
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 310
PaeI GCATGC 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 4
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
Sau3AI GATC 3 cut(s) 164, 190, 205
Sau96I GGNCC 1 cut(s) 4
SduI GDGCHC 1 cut(s) 263
SetI ASST 6 cut(s) 59, 107, 186, 231, 319, 324
SfcI CTRYAG 1 cut(s) 160
Sfr274I CTCGAG 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 74
SmlI CTYRAG 3 cut(s) 74, 199, 280
SmoI CTYRAG 3 cut(s) 74, 199, 280
SphI GCATGC 1 cut(s) 310
Sse9I AATT 2 cut(s) 246, 349
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 1 cut(s) 116
SspMI CTAG 1 cut(s) 68
StuI AGGCCT 1 cut(s) 269
TaiI ACGT 1 cut(s) 186
TaqI TCGA 1 cut(s) 75
TasI AATT 2 cut(s) 246, 349
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 1 cut(s) 127
VneI GTGCAC 1 cut(s) 259
XceI RCATGY 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 74
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.